• Journal of Environmental Health Sciences
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• v.37 no.2
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• pp.165-169
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• 2011

Between January 2010 and March 2011, there were three outbreaks of foot and mouth disease (FMD) in South Korea. Over 3.45 million animals (5,660 farms) were slaughtered, which was 33.3% of the existing pigs, 8.4% of dairy cows and 3.4% of cattle. FMD disaster costs were estimated at around three billion Korean won. Nine civil servants were killed, over 150 people were wounded and 4,788 landfills were confronted with a pollution problem. Vaccination and slaughter are the two basic alternatives for eradication of FMD. Altho ugh slaughter is more violent, risky and expensive than vaccination, the Korean government had chosen only slaughter eradication by the end of 2010. Even though over three million animals were killed, FMD spread out over most of the country. Finally, the government chose to begin vaccination. Following vaccination, outbreaks decreased dramatically. The purpose of this report is a cultural analysis of the related decision-making process, laws and systems. For the culture analysis, we utilize interviews, symposiums, laws, FMD manual, government reports and press releases. In conclusion, we found that the FMD massacre was influenced by cultural and organizational factors. The cultural factors were economism, cheapening of the value of life, biased perceptions and fears. The organizational factors were a closed process of decision-making, monopoly system, a small homogeneous group and group-think. Therefore, more studies will be needed for those factors of FMD disasters in national-scale cases.

• Korean Journal of Veterinary Research
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• v.21 no.2
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• pp.151-159
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• 1981

An investigation on various causes of milking disturbances resulting from injuries and abnormalities of mammary system were made in 2,179 Holstein cows. To perform this investigation, 69 dairy farms of the suburban area of Seoul city, Gyeonggi-do and Chungnam provinces were andomly selected and subjected. Diagnosis was made by means of inspection and palpation of teat, insertion of teat canula, checking milk machines, anamneses and farm records. This investigation was, actively done from March 1977 to February 1979, The results obtained were summerized as follows; 1. It was found that 446 cows, accounting for 20.48% of 2,179 cows inspected, had supernumerary teats. Among them 53.59% had one, 43.72% had two, 2.47% had three, and 0.22% had four supernumerary teats, respectively. 2. Dry off quarters were found in 158 quarters which turned out to be 1.8% of 8,716 quarters inspected. Among dry off quarters, 62.02% seemed to be caused by mastitis, 30.37% by acquired teat obstructions, and 7.59% by congenital blind teats and glands respectively. 3. Teat sphincter stenosis was found in 154 teats of 50 cows, which represents 1.76% of 8,716 teats and 2.29% of 2,179 cows inspected, respectively. Among 154 teats with teat sphincter stenosis, 138 teats (85.7%) of 33 cows were found to be congenital and revealed highest incidence. 4. Loose sphincter was found in 78 teats of 36 cows, which figure 0.89% of 8,7l6 teats and 1.69% of 2,179 cows inspected, respectively. Among 78 teats with loose sphincter, 52 teats (66.66%) of 13 cows were found to be congenital and revealed highest incidence. 5. Injured teat tip caused by over milking of milk machine, was found in 229 teats of 156 cows, which figure 2.63% of 8,716 teats and 7.15% of 2,179 cows observed, respectively. 6. Other miscellaneous injuries and congenital abnormalities of teats and udders were diagnosed as follows: The teat laceration was found in 34 teats (0.39% of 8,716 teats), fissure of teat skin in 24 teats (0.28% of 8,716 teats), stricture of teat cistern in 21 teats (0.24% of 8,716 teats), teat fistula in 12 teats (0.14% of 8,716 teats), papillomas on testes in 8 teats (0.09% of 8,716 teats). Knothole orifice in 7 teats (0.08% of 8,716 teats), subcutaneous abscess of udder in 5 quarters (0.05% of 8,716 teats), membraneous obstruction of teat cistern in 4 teats (0.05% of 8,716 teats), and congenital short teat in 8 teats (0.09% of 8,716 teats), respectively.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1251-1251
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• 2001

The routine analysis of milk chemical components is of major importance both for the management of animals in dairy farms and for quality control in dairy industries. NIRS technology is an analytical technique which greatly simplifies this routine. One of the most critical aspects in NIRS analysis of milk is sample preparation and analysis modes which should be fast and straightforward. An important difficulty when obtaining NIR spectra of milk is the high water content (80 to 90%) of this product, since water absorbs most of the infrared radiation, and, therefore, limits the accuracy of calibrating for other constituents. To avoid this problem, the DESIR system was set up. Other ways of radiation-sample interaction adapted for liquids or semi-liquids exist, which are practically instantaneous and with limited or null necessity of sample preparation: Transmission and Folded Transmission or Transflectance. The objective of the present work is to compare the precision and accuracy of milk calibration equations in two analysis modes: Reflectance (dry milk) and Folded Transmission (liquid milk). A FOSS-NIR Systems 6500 I spectrophotometer (400-2500 nm) provided with a spinning module was used. Two NIR spectroscopic methods for milk analysis were compared: a) folded transmission: liquid milk samples in a 0.1 pathlength sample cell (ref. IH-0345) and b) reflectance: dried milk samples in glass fibre filters placed in a standard ring cell. A set of 101 milk samples was used to develop the calibration equations, for the two NIR analysis modes, to predict casein, protein, fat and dry matter contents, and 48 milk samples to predict Somatic Cell Count (SCC). The calibrations obtained for protein, fat and dry matter have an excellent quantitative prediction power, since they present $r^2$ values higher than 0.9. The $r^2$ values are slightly lower for casein and SCC (0.88 and 0.89 respectively), but they still are sufficiently high. The accuracy of casein, protein and SCC equations is not affected by the analysis modes, since their ETVC values are very similar in reflectance and folded transmission (0.19% vs 0.21%; 0.16% vs 0.19% and 55.57% vs 53.11% respectively), Lower SECV values were obtained for the prediction of fat and dry matter with the folded transmission equations (0.14% and 0.25% respectively) compared to the results with the reflectance ones (0.43% and 0.34% respectively). In terms of accuracy and speed of analytical response, NIRS analysis of liquid milk is recommended (folded transmission), since the drying procedure takes 24 hours. However, both analysis modes offer satisfactory results.

• The Journal of Korean Society of Virology
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• v.26 no.2
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• pp.181-189
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• 1996

Fecal samples of calf diarrhea were taken on farms in Jeju island, rotavirus was isolated and cytopathic effect (CPE) was determined after infection to MA104 cell. Morphological evaluation on electron microscopy proved it as rotavirus. Also, its infection to MA104 cell was reidentified using a fluorescence antibody method. Genotype of Jeju island bovine rotavirus (JBR) analyzed through PAGE was 4: 2: 3: 2 pattern, which was unique in bovine and that analyzed through general PAGE was somewhat different from NCDV, UK, KK3, A5-3A, 61A, B223 and similar to N stool-5, N culture-5 and Kawatabi (Japan). By titration after plaquing, the level was $1-3\;{\times}\;10^6\;PFU/ml$, which was lower than those of NCDV and UK. Electrophoresis analysis of RNA-RNA hybridization, ELISA, and first and second PCR products of VP7 and VP4 in 1% agarose ($TAE+1{\mu}l$ EtBr) revealed that the rotavirus was a serotype of G6P11.

• Korean Journal of Veterinary Research
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• v.29 no.3
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• pp.383-391
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• 1989

This study was carried out to diagnostic test for bovine mastitis by the determination of adenosine triphosphate (ATP) based on firefly bioluminescence. The results obtained are follow; 1. The infection rate of bovine mastitis investigated with 521 cows in 47 dairy farms were found to be 3.6% of clinical form and 44.1% of subclinical form according to the degree of infection. 2. The light yield produced in firefly bioluminescence system was proportional to the concentration of ATP giving stright line within the range of 100PM~1uM. 3. When the number of somatic cell in milk was determined by the ATP assay and compared with three conventional methods such Fossomatic, California mastatic test (CMT), and rolling ball viscometer (RBV), it was shown that r=0.92 for Fossomatic, 0.63 for CMT and 0.7 for RBV. 4. The microorganisms causing mastitis were isolated Staphylococcus sp. (53.3%), Streptococcus sp. (17.9%), Micrococcus sp. (13.5%), Gram negative bacilli (6.3%), Gram positive bacilli (5.5%) and Yeast-like fungi (5.4%). 5. The endogeneous ATP levels of bacteria in a raw milk determined by the firefly bioluminescence system and compared with the results of the conventional methods. The correlation was 0.88 for raw milk.

• Korean Journal of Veterinary Service
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• v.15 no.2
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• pp.203-214
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• 1992

Livestock waste causes ground and surface water pollution, eutrophication of reservoir as well as adverse affects living environment of those who dwell nearby. In order to investigate the pollution load from livestock waste, physical and chemical characteristics of the waste were determinded in the survey of livestock farms. Once pollution load is obtained as a function of the origin units of livestock, the influence of livestock waste on the Daecheong reservoir was studied. 1. For Daecheong reservoir inflow area, the liverstock manure productions of beef cattle swine, dairy cattle and chicken were 1,135.6t/day, 480t/day, 241.3t/day, 48t/day respectively； Beef cattle was the mai or source of the pollution. Pollution loading productions due to the livestock waste around the were found to be 53.31t/day for BOD, 222.49t /day for COD, 261.99t/day for T-S, 9.64t/day for T-N, 6.54t/day for T-P 2. Bocheong stream turnd out to be the major contribution for pollution loading production to Daecheong reservoir with 10,748kg /day of BOD, 47,157kg /day of COD, 1,946kg /day of T-N, 1,271kg /day of T-p. 3. Actual pollution loadings from livestock wastes for the area of Daecheong reservoir were estimated as 1,997kg /day(BOD), 8,546kg /day(COD), 364kg /day(T-N), 243kg /day (T-P), respectively Therefore, advanced treatment for livestock waste is recommended for Daectleong reservoir inflow area to eliminate the nutrients which are major sources of eutrophication of the reservoir.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.22 no.4
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• pp.309-315
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• 2012

Objectives: Animal husbandry workers could be exposed to various work hazards including toxic gases, chemicals such as pesticides or organic dust. Immunological evaluation focusing on respiratory allergic hypersensitivity occurrence was under-taken for swine farm workers as a part of the study on immunologic status of dairy barn, swine confinement, and poultry farm workers. Materials and Methods: Peripheral bloods were collected from 25 workers at the year of 2001 and 12 workers at the year of 2012 from swine farms located at Gyeonggi province, Korea. Seven adults not involved with animal husbandry were recruited at the year of 2001 from the same residential area as the swine farm workers'. Level of plasma IgE and 20 respiratory allergen-specific IgE were evaluated using commercially available ELISA kit. Results: Plasma IgE level was approximately five-fold higher in the swine farm workers regardless of the sampling year than the control subjects. Plant allergens from outdoor environments such as golden rod, pigweed, Russian thistle, or ragweed were the major allergens with positive reaction(allergen specific IgE${\geq}$0.7 IU/mL) for the swine farm workers at 2001 year. Meanwhile, house dust mite(Dermatophagoides farinae, D. pteronyssinus) and cockroach, typical indoor allergens in Korea, were the major respiratory allergens for the swine farm workers at 2012 year. Conclusions: Overall, even though our results are primitive, the results suggest that immunological function of swine farm workers could be modulated toward type-2 reactivity.

• Korean Journal of Veterinary Service
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• v.39 no.3
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• pp.159-166
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• 2016

Clostridium perfringens, Salmonella thyphimurium and S. Montevideo isolated from the intestines of dead broiler chickens in Korea were tested for antibacterial effects to purifed bee venom. Purified bee venom from Apis mellifera L. has been used as natural antimicrobial compounds in pigs, cows, dairy cattle and chicken farms in Korea. To investigate antibacterial effect of purified bee venom was evaluated by agar well diffusion method, minimum inhibitory concentraion (MIC), minimum bactericidal concentration (MBC), and postantibiotic effect (PAE). Purified bee venom exhibited significant inhibition of bacterial growth of C. perfringens, S. thyphimurium and S. Montevideo with MIC value of 0.85, 0.68 and $0.69{\mu}g/mL$, respectively. The MBC value of purified bee venom against C. perfringens, S. thyphimurium and S. Montevideo were 3.33, 2.66 and $2.86{\mu}g/mL$. Furthermore, the results of PAE values against C. perfringens, S. thyphimurium and S. Montevideo showed the bacterial effect with 3.5, 4.0 and 3.5 hr. Stability of pufifed bee venom at acidity from pH 1 to pH 8 for 24 hr was the antibacterial activity for C. perfringens, S. thyphimurium and S. Montevideo and melittin contents. Also purified bee venom processed through the heating for 15 min, there was no signification loss of the antibacterial activity and melittin at below $100^{\circ}C$. These results obtained in this study suggest that purified bee venom might be utilized as a feed additive in poultry diets.

• Korean Journal of Veterinary Research
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• v.24 no.1
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• pp.91-98
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• 1984

A total of 466 foremilk from dairy farms in Chonnam district was examined for the subclinical mastitis over a period of one year, using a method of the electrical conductivities(EC); absolute conductivity(AC) and differential conductivity(DC) and quarter difference value(QD), in relation to the California mastitis test(CMT) and the direct somatic cell count(DSCC). The compatibility and efficiency rating between the EC values and the other screening tests was conducted. Obtained results are summarized as follows. 1. A linear relationship was found between the EC values and the CMT scores and direct somatic cell counts and it was found that electrical conductivity measurements were comparable with other screening tests for diagnosing animals with mastitis. 2. Compatibilities between the EC and CMT were 70.4% in AC, 74.6% in DC and 70.7% in QD, and that of the EC and DSCC were 53.0% in AC, 63.1% in DC and 53.2% in QD. On the other hand, relative efficiency ratings of Postle's equation between EC and CMT were 37.3% in AC, 26.5% in DC and 13.6% in QD, and that of the EC and DSCC were 33.1% in AC, 20.2% in DC and 11.9% in QD. 3. In the foremilk samples collected from damaged quarters determined by EC, the false positive rate wart higher than the false negative rate, and consequently tests of EC produced lower compatibility or efficiency rating scores. These tendencies suggested that any factors other than the mastitic condition influencing the EC values might be existed.

• Korean Journal of Veterinary Service
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• v.39 no.2
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• pp.81-86
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• 2016

Honeybee venom (BV) from Apis mellifera L. has been used as natural antimicrobial compounds in pigs, cows, dairy cattle and chicken farms in Korea. The purpose of this study was conducted to confirm homogeneity and stability of BV dissolved with distilled water or saline solution. Melittin was analyzed with ultra-high performance liquid chromatography (UPLC) for BV to secure the validation of analysis. BV at concentration of 1 mg/mL was dissolved with distilled water or saline solution at room temperature. Homogeneity of BV dissolved with distilled water or saline solution at upper, milddle, and lower layers all satisfied the accuracy and precision criteria. Stability of BV dissolved with distilled water or saline solution for 7 days all satisfied the criterion both light and dark storage condition. BV has satisfied with homogeneity and stability in distilled water or saline solution at room temperature under light or dark condition. The results of this study suggest that BV has a possibility as the substitute of natural antimicrobial agents for the animal drugs and feed additives.