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http://dx.doi.org/10.7853/kjvs.2016.39.2.81

Experimental studies of homogeneity and stability honeybee venom using ultra-high performance liquid chromatography  

Han, Sang Mi (Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Kim, Se Gun (Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Hong, In Phyo (Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Woo, Soon Ok (Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Jang, Hye Ri (Department of Agricultural Biology, National Institute of Agricultural Science, RDA)
Lee, Kyung Woo (Department of Animal Science and Technology, Konkuk University)
Publication Information
Korean Journal of Veterinary Service / v.39, no.2, 2016 , pp. 81-86 More about this Journal
Abstract
Honeybee venom (BV) from Apis mellifera L. has been used as natural antimicrobial compounds in pigs, cows, dairy cattle and chicken farms in Korea. The purpose of this study was conducted to confirm homogeneity and stability of BV dissolved with distilled water or saline solution. Melittin was analyzed with ultra-high performance liquid chromatography (UPLC) for BV to secure the validation of analysis. BV at concentration of 1 mg/mL was dissolved with distilled water or saline solution at room temperature. Homogeneity of BV dissolved with distilled water or saline solution at upper, milddle, and lower layers all satisfied the accuracy and precision criteria. Stability of BV dissolved with distilled water or saline solution for 7 days all satisfied the criterion both light and dark storage condition. BV has satisfied with homogeneity and stability in distilled water or saline solution at room temperature under light or dark condition. The results of this study suggest that BV has a possibility as the substitute of natural antimicrobial agents for the animal drugs and feed additives.
Keywords
Honeybee venom; Melittin; UPLC; Homogeneity; Stability;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 한상미, 이광길, 여주홍, 우순옥, 권해용. 2007. 봉독 간이정제법. 특허 제 10-0758814호
2 식품의약품안전청 2008. 식품의약품안전청 고시 제2008-5호. 의약품 등 밸리데이션 실시에 관한 규정
3 Curcio-Vonlanthen V, Schneider CH, Frutig K, Blaser K, Kalbacher H. 1997. Molecular parameters in melittin immunogenicity. J Pept Sci 3(4): 267-276.   DOI
4 Fennell JF, Shipman WH, Cole LJ. 1967. Antibacterial action of a bee venom fraction (melittin) against a penicillin-resistant Staphylococcus and other microorganisms. Res. Dev Tech Rep 5: 1-13.
5 Habermann E, Reiz KG. 1965. On the biochemistry of bee venom peptides, melittin and apamin. Biochem Z 343(2): 192-203.
6 Han SM, Lee KG, Yeo JH, Kweon HY, Woo SO, Lee ML, Baek HJ, Kim SY, Park KK. 2007. Effect of honey bee venom on microglial cells nitric oxide and tumor necrosis factor-alpha production stimulated by LPS. J. Ethnopharmacol 111(1): 176-181.   DOI
7 Kim JY, Lee WR, Kim KH, An HJ, Chang YC, Han SM, Park YY, Pak SC, Park KK. 2015. Effects of bee venom against Propionibacterium acnes-induced inflammation in human keratinocytes and monocytes. Int J Mol Med 35: 1651-1656.   DOI
8 Han SM, Lee KG, Yeo JH, Kweon HY, Kim BS, Kim JY, Baek HJ, Kim ST. 2007. Antibacterial acitivity of the honey bee venom against bacterial mastitis pathogens infecting dariy cows. Int J Indust Entomol 14(2): 137-142.
9 Han SM, Lee KG, Yeo JH, Hwang SJ, Chenoweth PJ, Pak SC. 2009a. Effects of bee venom treatment on growth performance of young pigs. Am J Chin Med 37(2): 833-842.
10 Han SM, Lee KG, Yeo JH, Hwang SJ, Chenoweth PJ, Pak SC. 2009b. Somatic cell count in milk of bee venom treated dairy cows with mastitis. J Api Product ApiMed Sci 1(4): 104-109.   DOI
11 Han SM, Lee KG, Yeo JH, OH BY, Kim BS, Lee W, Baek HJ, Kim ST, Hwang SJ, Pak SC. 2010a. Effects of honeybee venom supplementation in drinking water on growth performance of broiler chickens. Poult Sci 89: 2396-2400.   DOI
12 Piek T. 1986. Venoms of the Hymenoptera. Academic press, London. United kigdom.
13 Han SM, Lee KG, Yeo JH, Oh BY, Kim ST. 2010b. Effects of honeybee (Apis mellifera L.) venom on the reproductive efficiency of dams and the growth performance, disease occurrence of Hanwoo calves. Korean J Vet Serv 33(3): 287-292.
14 Han SM, Hong IP, Woo SO, Kim SG, Jan HR. 2015. Analysis of bee venom residues in milks of dairy cattle using UHPLC with newly developed pre-processing method. Korean J Vet Serv 38(1): 25-30.   DOI