Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Experimental studies of homogeneity and stability honeybee venom using ultra-high performance liquid chromatography

주사제 사용을 위한 봉독의 균질성 및 안정성 평가

  • Han, Sang Mi (Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Kim, Se Gun (Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Hong, In Phyo (Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Woo, Soon Ok (Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Jang, Hye Ri (Department of Agricultural Biology, National Institute of Agricultural Science, RDA) ;
  • Lee, Kyung Woo (Department of Animal Science and Technology, Konkuk University)
  • 한상미 (국립농업과학원 농업생물부) ;
  • 김세건 (국립농업과학원 농업생물부) ;
  • 홍인표 (국립농업과학원 농업생물부) ;
  • 우순옥 (국립농업과학원 농업생물부) ;
  • 장혜리 (국립농업과학원 농업생물부) ;
  • 이경우 (건국대학교 동물자원과학과)
  • Received : 2016.03.14
  • Accepted : 2016.05.23
  • Published : 2016.06.30
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Abstract

Honeybee venom (BV) from Apis mellifera L. has been used as natural antimicrobial compounds in pigs, cows, dairy cattle and chicken farms in Korea. The purpose of this study was conducted to confirm homogeneity and stability of BV dissolved with distilled water or saline solution. Melittin was analyzed with ultra-high performance liquid chromatography (UPLC) for BV to secure the validation of analysis. BV at concentration of 1 mg/mL was dissolved with distilled water or saline solution at room temperature. Homogeneity of BV dissolved with distilled water or saline solution at upper, milddle, and lower layers all satisfied the accuracy and precision criteria. Stability of BV dissolved with distilled water or saline solution for 7 days all satisfied the criterion both light and dark storage condition. BV has satisfied with homogeneity and stability in distilled water or saline solution at room temperature under light or dark condition. The results of this study suggest that BV has a possibility as the substitute of natural antimicrobial agents for the animal drugs and feed additives.

Keywords

References

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