• Korean Journal of Pharmacognosy
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• v.19 no.4
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• pp.233-238
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• 1988

Angelica flaccida Kom. is a herbal plant growing wild in the marsh of valley in Korea. It has been used for sedative and analgesic as a folk medicine, but its constituents have not been clarified yet. Five known coumarins such as isoimperatorin, decursidin, (-) anomalin, decursin, decursinol and two sterols such as ${\beta}-sitosterol$ and stigmasterol were isolated from the root of this plant, Their structures were identified by the UV, IR, NMR, mass data and the physico-chemical properties.

• Korean Journal of Pharmacognosy
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• v.25 no.4 s.99
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• pp.311-318
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• 1994

From the root of Angelica decursiva-albiflora Yook, which has been used as a folk medicine for a sedative, analgesic and expectorant, four free coumarins, e.g., decursidin(I), decursin(II), umbelliferone(III) and nodakenetin(IV), and two coumarin glycosides, e.g., nodakenin(V) and decuroside I(VI) were isolate. The cytotoxicity of nodakenin(V) against L-1210 leukemia cells was less effective than cisplatin, but in the nephrotoxicity against rabbit kidney proximal tubular cell nodakenin(V) showed remarkably less nephrotoxicant than cisplatin.

• Korean Journal of Pharmacognosy
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• v.2 no.1
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• pp.29-34
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• 1971

A comparative study on Angelica and Cnidium, the two genera of Umbelliferae growing in Korea, was carried about the cytology and fertility of pollen. The somatic chromosome number of Umbelliferae was found almost to be 2N=22. Both species of Kang-whal and Chun-gung were found to be in polyploid with different chromosome numbers each other. The pollen fertility among the species of Angelica was high, but Toh-chun-gung was zero percent. The species of Angelica have been generated with pollen fertility, but species of Cnidium with only root or rootlet.

• Korean Journal of Pharmacognosy
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• v.21 no.1
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• pp.64-68
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• 1990

From the root of Angelica gigas Nakai, decursinol angelate was isolated, which is structural isomer of decursin. Raw material contained average 2.44% of decursinol angelate and 3.30% of decursin by the GC analysis.

• Korean Journal of Pharmacognosy
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• v.2 no.2
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• pp.87-88
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• 1971

Silica gel column chromatography on the ether extract from the fruits of Angelica tenuissima $N_{AKAI}$ gave three kinds of crystalline constituents. The following derivatives of furocoumarins and steroid were identified by UV, IR, NMR spectra, elemental analysis and physico-chemical tests: iso-imperatorin m.p. $108{\sim}109^{\circ}\;C_{16}H_{14}O_4$, prangolarine m.p. $103{\sim}104^{\circ}\;C_{16}H_{14}O_5$, ${\beta}-sitosterol$ m.p. $138{\sim}139^{\circ}\;C_{29}H_{50}O$.

• Korean Journal of Pharmacognosy
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• v.28 no.2
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• pp.80-83
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• 1997

The methanol extract of aerial part of Angelica keiskei Koidzumi exhibited a strong antimutagenic activity against aflatoxin $B_1\;(AFB_1)$. N-methyl-N'-nitro-N-nitrosoguanidine and 4-nitroquinoline-1-oxide in the Ames test with Salmonella typhimurium TA100. Cynaroside, isolated front ethylacetate fraction of the methanol extract Over silica gel, inhibited the mutagenicity of $AFB_1$ with an inhibition value of 96% at 1.0 mg/plate concentration and 87% at 0.5 mg/plate concentration. Other compounds, hyperoside, sucrose and luteolin-7-rutinoside, isolated from ethylactate or n-butanol fraction, also showed antimutagenic effect.

• Korean Journal of Medicinal Crop Science
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• v.22 no.2
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• pp.91-97
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• 2014

The contents of index components in 5 medicinal crops cultivated at different heights in Andong area. The contents of allantoin in Dioscorea batatas Decne and catalpol in Rehmannia glutinosa Liboschitz were not different by cultivation heights (100 ~ 200m). But the contents of index components in Astragalus membranaceus and Angelica acutiloba Kitagawa were higher in samples cultivated at elevated heights. At 700 m of heights, the contents of formononetin and astragaloside I in Astragalus membranaceus were 1.20 and $201.2{\mu}g/g$, respectively. The contents of decursin and nodakenin in Angelica acutiloba Kitagawa were 68.3 and 4.20 mg/g, respectively. Thus Astragalus membranaceus and Angelica acutiloba Kitagawa are recommended to be cultivated in higher altitude.

• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.12-18
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• 2017

The dried root of Angelica species is used in traditional Chinese medicine in East Asia, particularly in Korea, China and Japan. Since the plant origin differs in these countries, they are often misused or adulterated in the commercial markets, resulting in distrust among the consumers. Enormous efforts have therefore been focused to distinguish the origin for the Angelica genus, by using morphological or cytogenetical analyses, and chemical markers based on biochemical analyses of secondary metabolites. DNA is considerably stable against different cultivation conditions, and to treatment and processing after harvesting of plants. Hence, several researches have been filed for the development of molecular markers, based on the single nucleotide polymorphisms in specific regions of DNA. However, there are several obstacles for application in the commercial markets, concerning the reproducibility, accuracy, sensitivity, and rapidity of these tests. In this review, we summarize the research achievements that help classify the origin of Angelica species, in particular, Angelica gigas Nakai. A. sinensis(oliv.) Diels, A. acutiloba Kitag., and A. acutiloba var. sugiyamae Hikino. Further researches are required for practical applications.

• Journal of Plant Biotechnology
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• v.43 no.2
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• pp.151-156
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• 2016

Medicinal plants resources are becoming important assets since their usages have been expanded to the development of functional foods for human health, natural cosmetics, and pharmaceutical industries. However, names are different from each country and their phylogenetic origins are not clear. These lead consumers to be confused. In particular, when they are morphologically similar and distributed as dried roots, it is extremely difficult to differentiate their origins even by specialists. Recently, molecular markers have been extensively applied to identify the origin of many crops. In this review, we tried to overview the current research achievements for the development of suitable 'origin identification' regarding to the differentiation of Angelica species. Furthermore, more advanced techniques including amplification genome based marker analyses are also discussed for their practical applications in the authentication of particular medicinal plant in Angelica species.

• Journal of Applied Biological Chemistry
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• v.50 no.4
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• pp.264-269
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• 2007

Root extracts of Angelica gigas and A. acutiloba have been used traditionally for the treatment of gynecological diseases, as well as anemia, blood stasis, and inflammatory pain, as blood tonics in Oriental medicine. In the present study, we investigated the effects of A. gigas and A. acutiloba on inflammatory mediators in mouse macrophages and compared their activities. Many studies suggest that prostaglandin $E_2$ ($PGE_2$) biosynthesis and nitric oxide (NO) production play important roles in the processes of both inflammation and carcinogenesis. Ethanolic extracts from the roots of both species exhibited significant inhibitory effects on $PGE_2$ generation in lipopolysaccharide-stimulated RAW 264.7 cells. In particular, the extract from A. gigas was more effective than that from A. acutiloba. Although neither inhibited NO generation, the extract from A. acutiloba stimulated NO generation. Our results suggest that the roots of A. gigas might possess more anti-inflammatory and/or cancer chemopreventative activity than that of A. acutiloba due to the suppression of cyclooxygenase-2 (COX2)-mediated $PGE_2$ production. In addition, A. acutiloba might exert anti-tumor activity through an increase in macrophage-produced NO.