• The Plant Pathology Journal
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• 제25권1호
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• pp.70-76
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• 2009

The rhizobacteria of Panax notoginseng were isolated from six sites in Yanshan, Maguan and Wenshan Counties, Yunnan Province of China, and their antagonistic activity against P. notoginseng root-rot fungal pathogens was determined. Of the 574 rhizobacteria isolated, 5.8% isolates were antagonistic in vitro to at least one of the five pathogens, Cylindrocarpon didynum, Fusarium solani, Phytophthora cactorum, Phoma herbarum, and Rhizoctonia solani. The number of rhizo bacteria and the number that inhibited fungi differed depending on sampling sites and isolation methods. Rhizobacteria isolated from the site in Yanshan and Maguan showed more antagonistic effect than them in Wenshan. Heat treatment of rhizosphere soil at $80^{\circ}C$ for 20 min scaled the antagonists up to 14.0%. Antagonistic bacteria in the roots proportioned 3.9% of the total isolates. The most antagonistic isolates 79-9 and 81-4 are Bacillus subtilis based on their 168 rDNA sequence and biochemical and physiological characteristics. Identification and evaluation of antagonistic bacteria against P. notoginseng root-rot pathogens in the main planting areas improved our understanding of their distribution in rhizosphere soil. Furthermore these results indicated that the interactions between biocontrol agent and soil microbes should be seriously considered for the successful survival and biocontrol efficacy of the agents in soil.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 1999년도 정기총회 및 춘계 학술발표회
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• pp.26.1-26
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• 1999

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2000년도 추계 학술발표회
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• pp.28.1-28
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• 2000

• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2005년도 춘계 학술발표회
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• pp.71-71
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• 2005

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 1999년도 정기총회 및 춘계 학술발표회
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• pp.48.3-49
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• 1999

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2002년도 총회 및 추계학술발표회
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• pp.52.2-52
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• 2002

• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.208-216
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• 2010

Fusarium verticillioides is an important pathogen of maize, being responsible for ear rots, stalk rots, and seedling blight worldwide. During the past decade, F. verticillioides has caused several severe epidemics of maize seedling blight in many areas of China, which lead to significant losses. In order to understand the molecular mechanisms regulating fungal development and pathogenicity in this pathogen, we isolated and characterized the gene fpk1 (GenBank Accession No. EF405959) encoding a homolog of the cAMP-dependent protein kinase catalytic subunit, which included a 1,854-bp DNA sequence from ATG to TAA, with a 1,680-bp coding region, and three introns (lengths: 66 bp, 54 bp, and 54 bp), and the predicated protein precursor had 559 aa. The mutant ${\Delta}fpk1$, which was disrupted of the fpkl gene, showed reduced vegetative growth, fewer and shorter aerial mycelia, strongly impaired conidiation, and reduced spore germination rate. After germinating, the fresh hypha was stubby and lacking of branch. When inoculated in susceptible maize varieties, the infection of the mutant ${\Delta}fpk1$ was delayed and the infection efficiency was reduced compared with that of the wild-type strain. AU this indicated that gene fpk1 participated in hyphal growth, conidiophore production, spore germination, and virulence in F. verticillioides.

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 2003년도 춘계학술발표대회 요약집
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• pp.81-81
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• 2003

• 농약과학회지
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• 제14권3호
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• pp.284-288
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• 2010

전남 지역에서의 완두콩바구미 방제 약제 선발과 방제 시기 결정을 위한 일련의 실험이 포장과 실내에서 수행되었다. 1차 전남 농업기술원 포장 실험 결과 처리 약제는 카보푸란 입제는 50%의 방제가를 보였으며, 그외 시험 약제 fenitrothion, diazinon, carbaryl, ethofenprox, chlorpyrifos-methyl, spinosad, imidacloprid 등은 87.5% 이상의 방제가를 보였다. 방제시기와 처리 회수 실험에서는 결협기를 10일 간격으로 초, 종, 말기로 구분하여 약제를 처리한 결과 모든 약제에서 2회 처리(결협 초기, 증가)와 3회 처리(결협 초기, 중기, 말기) 시 fenitrothion(방제가 60.8%)를 제외한 diazinon, $\lambda$-cyhalothrin, chlorpyrifosmethyl, spinosad, imidacloprid 등은 86.3% 이상의 높은 방제 효과를 보였다. 방제는 결협 종기 (5월 10일 경)에 살포하는 것이 가장 높은 효과를 보였다. 완두콩바구미의 방제는 결협 10일 후에 방제 약제를 한 번 살포하거나 4-5일 후에 다시 한 변 살포하여 두 번 살포하는 방제법이 가장 높은 방제 효과를 보일 것으로 사료된다. 실내 실험에서 완두콩바구미는 거의 모든 유기인계에 매우 높은 감수성을 보였다.

• Journal of Microbiology and Biotechnology
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• 제31권10호
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• pp.1373-1382
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• 2021

Plant growth promoting rhizobacteria (PGPR) are a group of bacteria that can increase plant growth; but due to unfavorable environmental conditions, PGPR are biologically unstable and their survival rates in soil are limited. Therefore, the suitable application of PGPR as a plant growth stimulation is one of the significant challenges in agriculture. This study presents an intelligent formulation based on Bacillus velezensis VRU1 encapsulation enriched with nanoparticles that was able to control Rhizoctonia solani on the bean. The spherical structure of the capsule was observed based on the Scanning Electron Microscope image. Results indicated that with increasing gelatin concentration, the swelling ratio and moisture content were increased; and since the highest encapsulation efficiency and bacterial release were observed at a gelatin concentration of 1.5%, this concentration was considered in mixture with alginate for encapsulation. The application of this formulation which is based on encapsulation and nanotechnology appears to be a promising technique to deliver PGPR in soil and is more effective for plants.