• Journal of Animal Science and Technology
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• v.48 no.3
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• pp.393-400
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• 2006

The present study was conducted to evaluate feeding value of brown rice in Korean native beef steers (Hanwoo) fed concentrates and orchardgrass hay (50:50 on a wet basis) as a basal diet. Either 50% or 100% of corn in concentrates was substituted for brown rice, and ruminal fermentation characteristics, in situ degradability of feeds and in vivo digestibility of concentrate were investigated. The digestibility and nutritive values of corn and brown rice feeds were similar, whereas rumen degradability of brown rice was higher than that of corn. Compared with corn-based diets, the brown rice substitution decreased (P<0.05) ruminal NH3-N concentration, but did not affect rumen pH. Total volatile fatty acid content in ruminal digesta for the corn based diet was higher than those for the brown rice-contained diets. The brown rice substitution increased (P<0.05) the ratio of ruminal acetic acid to propionic acid (A/P) compared with corn-contained diets.Present results indicate that corn might nutritionally be substituted for brown rice as cattle feeds. In addition, we expect that relatively high A/P with brown rice may produce affirmative effects in rumen physiological aspect, particularly under the grain based feeding system for beef cattle in Korea.

• BMB Reports
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• v.41 no.12
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• pp.846-851
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• 2008

This study was conducted to identify marbling-related candidate genes in M. longissimus dorsi of high- and low-marbled Hanwoo. The longissimus dorsi muscles were selected for gene expression from eight Hanwoo steer carcasses based on crude fat content. In the analysis of variance, gene expression of five candidate genes, FABP4, SCD, $PPAR\gamma$, Titin and Nebulin was determined to be significantly different between high- and low-marbled Hanwoo steers (P < 0.0001). The Pik-4 and CaMK II genes were also shown to have a significant effect on crude fat content (P < 0.01). In the analysis of the differential expression between high- and low marbled groups, FABP4 gene expression was approximately 2 times higher in the high marbled group relative to the low marbled group. However, the $PPAR\gamma$ and SCD gene were highly expressed in the low marbled group. In addition, Titin and Nebulin were highly expressed in the low marbled group when placed under relatively high shear force. Finally, the Pik-4 and CaM K II gene also displayed a high expression pattern in the low marbled group.

• Journal of Animal Science and Technology
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• v.49 no.1
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• pp.41-50
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• 2007

Data from a comparative slaughter experiment with two hundreds of Korean native (Hanwoo) beef steers were utilized to determine net energy and protein requirements for growth (NEg and NPg). Eight randomly selected steers were used in the initial slaughter group of 6 month of age and the remaining steers allocated to treatments within two groups. The restricted groups were fed 1.2 to 1.5% concentrate per kg body weight (BW) in the growing period (6～12 month of age), 1.7 to 1.8% concentrate per kg BW in the early fattening period (13～18 month of age), and concentrate feed ad libitum in the late fattening period (19～30 month of age). Ad libitum groups were fed concentrate ad libitum through the whole period. Rice straw was offered ad libitum for 24 month of two feeding systems. The steers were slaughtered when animals reached every 2 month from 8 to 30 month of age. For all body composition determinations, whole empty body components were weighed, taken each proportional subsample was ground for chemical analysis. Equations developed with the pooled data to predicted NEg and NPg were similar to the equations of Japanese Feeding Standard for Beef Cattle (2000). First equations were developed to predicted NEg; NEg = 0.05332×LBW0.75×DG for restricted treatment and NEg = 0.04912×LBW0.75×DG for ad libitum treatment. Second equations were developed to predicted NPg; NPg = DG × (224.7－0.251×LBW) for restricted treatment and NPg=DG×(210.1－0.214×LBW) for ad libitum treatment.

• Food Science of Animal Resources
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• v.37 no.2
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• pp.168-174
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• 2017

We hypothesized that supplementing finishing diets with palm oil would promote adipogenic gene expression but depress stearoyl-CoA desaturase (SCD) gene expression in intramuscular (i.m.) adipose tissues of Hanwoo steers during fattening period (from 16 to 32 mon of age). Fourteen Hanwoo steers were allotted randomly to 2 groups of 7 steers based on initial BW and fed either a basal diet (control) or the basal diet supplemented with 5% palm oil (BDSP). At slaughter, i.m. adipose tissue was harvested for analysis of adipogenic gene expression and fatty acid composition. There were no differences in BW or average daily gain between treatment groups. Supplemental palm oil had no effect on carcass quality traits (carcass weight, backfat thickness, loin muscle area, or marbling scores) or meat color values. Palm oil increased (p<0.05) expression of AMP-activated protein kinase-${\alpha}$ and peroxisome proliferator-activated receptor-${\gamma}$, but decreased (p<0.05) CAAT/enhancer binding protein-${\beta}$ gene expression and tended to decrease stearoyl-CoA desaturase gene expression in i.m. adipose tissue. Palm oil increased total i.m. polyunsaturated fatty acids (p<0.05) compared to the control i.m. adipose tissue, but had no effect on saturated or monounsaturated fatty acids. Although there were significant effects of supplemental palm oil on i.m. adipose tissue gene expression, the absence of negative effects on carcass and meat characteristics indicates that palm oil could be a suitable dietary supplement for the production of Hanwoo beef cattle.

• Journal of Animal Science and Technology
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• v.44 no.5
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• pp.593-598
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• 2002

This experiment was prosecuted to estimate the carcass traits and their relationships in Hanwoo steers. The mean carcass weight(CWT), back fat thickness (BFT), meat, bone, intra- muscular fat (IMF) and eye muscle area (EMA) of steers were 300.27 kg, 9.23 mm, 58.99%, 12.92%, 28.08% and 74.00 $cm^2$, respectively. The pHB(before aging), pHA(after aging), cook loss before aging($CL_B$) and cook loss after aging ($CL_A$) were 5.32, 5.60, 16.43% and 19.21%, respectively. It was also found that the MS and FC were 3.67 and 3.17, respectively. The EMA and quantity index (QIX) were positively correlated with CWT, meat% and bone% but negatively correlated with IMF%. The BFT was positively correlated with CWT and meat% but negatively correlated with bone% and IMF%. The pH (both before and after aging) was negatively correlated with CL (both before and after aging), water loss during freezing (WLF) and FC. The muscle score(MS) was negatively correlated with CL but positively correlated with WLF and FC. The ARM, HRD, CHW and CTM increased with the increasing of fat% and sharply decreased with the increasing of meat% in the carcass.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.11
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• pp.1652-1662
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• 2014

The present study investigated the optimum blending condition of protected fat, choline and yeast culture for lowering of rumen temperature. The Box Benken experimental design, a fractional factorial arrangement, and response surface methodology were employed. The optimum blending condition was determined using the rumen simulated in vitro fermentation. An additive formulated on the optimum condition contained 50% of protected fat, 25% of yeast culture, 5% of choline, 7% of organic zinc, 6.5% of cinnamon, and 6.5% of stevioside. The feed additive was supplemented at a rate of 0.1% of diet (orchard grass:concentrate, 3:7) and compared with a control which had no additive. The treatment resulted in lower volatile fatty acid (VFA) concentration and biogas than the control. To investigate the effect of the optimized additive and feed energy levels on rumen and rectal temperatures, four rumen cannulated Hanwoo (Korean native beef breed) steers were in a $4{\times}4$ Latin square design. Energy levels were varied to low and high by altering the ratio of forage to concentrate in diet: low energy (6:4) and high energy (4:6). The additive was added at a rate of 0.1% of the diet. The following parameters were measured; feed intake, rumen and rectal temperatures, ruminal pH and VFA concentration. This study was conducted in an environmentally controlled house with temperature set at $30^{\circ}C$ and relative humidity levels of 70%. Steers were housed individually in raised crates to facilitate collection of urine and feces. The adaptation period was for 14 days, 2 days for sampling and 7 days for resting the animals. The additive significantly reduced both rumen (p<0.01) and rectal temperatures (p<0.001) without depressed feed intake. There were interactions (p<0.01) between energy level and additive on ruminal temperature. Neither additive nor energy level had an effect on total VFA concentration. The additive however, significantly increased (p<0.01) propionate and subsequently had lower acetate:propionate (A/P) ratios than non-additive supplementation. High concentrate diets had significantly lower pH. Interactions between energy and additive were observed (p<0.01) in ammonia nitrogen production. Supplementation of diets with the additive resulted in lower rumen and rectal temperatures, hence the additive showed promise in alleviating undesirable effects of heat stress in cattle.

• BMB Reports
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• v.39 no.2
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• pp.183-188
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• 2006

Using the Phred/Phrap/Polyphred/Consed pipeline established in the National Livestock Research Institute of Korea, we predicted candidate coding single nucleotide polymorphisms (cSNPs) from 7,600 expressed sequence tags (ESTs) derived from three cDNA libraries (liver, M. longissimus dorsi, and intermuscular fat) of Hanwoo (Korean native cattle) steers. From the 7,600 ESTs, 829 contigs comprising more than two EST reads were assembled using the Phrap assembler. Based on the contig analysis, 201 candidate cSNPs were identified in 129 contigs, in which transitions (69%) outnumbered transversions (31%). To verify whether the predicted cSNPs are real, 17 SNPs involved in lipid and energy metabolism were selected from the ESTs. Twelve of these were confirmed to be real while five were identified as artifacts, possibly due to expressed sequence tag sequence error. Further analysis of the 12 verified cSNPs was performed using the program BLASTX. Five were identified as nonsynonymous cSNPs, five were synonymous cSNPs, and two SNPs were located in 3'-UTRs. Our data indicated that a relatively high SNP prediction rate (71%) from a large EST database could produce abundant cSNPs rapidly, which can be used as valuable genetic markers in cattle.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.5
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• pp.722-730
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• 2016

The carcass traits and meat quality of Hanwoo (Korean native cattle) whose diets were supplemented with direct-fed microbial (DFM) and pine cone extract (PCE) were evaluated. Twenty head of Hanwoo steers were divided equally into four groups and for a period of 6 months were given different diets: One group was fed a basal diet as control (CON), the other three groups were fed a basal diet supplemented with DFM-1%, DFM+PCE-1% and DFM+PEC-3%, respectively. DFM+PCE3% diet resulted the lowest carcass quality grade. The loins of DFM-1% contained higher moisture and lower fat than did the loins from the CON group. The crude protein content of DFM+PCE-3% group was significantly higher than that of the other groups. The water holding capacity and Warner-Bratzler shear force of the DFM+PCE-1% and 3% groups were lower than those of the CON and DFM-1% groups. The DFM-1% and 3% groups contained lower saturated fatty acid, higher unsaturated fatty acid, mono-unsaturated fatty acid, and poly-unsaturated fatty acid than did CON and DFM+PCE 1% group. Moreover, the n6:n3 ratios of DFM-1% and DFM+PCE-1% and 3% groups were slightly lower than that of the CON group. Thus we concluded that DFM and PCE supplementation resulted healthier Hanwoo beef with lower fat content and n6:n3 ratio.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.6
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• pp.898-907
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• 2005

Chromium has emerged as an essential trace mineral in nutrition. However, it readily causes toxicity because of slightly excessive dose and/or form of chromium supplement. Therefore, developing a noble form of chromium supplement which is capable of not only an increased availability but also a reduced toxicity has been a critical issue in chromium nutrition. Chromium-methionine chelate has been, so far, one of the latest developments in its kind. Although not much information is available for the chromium-methionine chelate, especially in view of animal performances upon dietary supplementation, several studies indicated chromium methionine chelate could be effective to improve meat quality by increasing muscle mass but decreasing body fat. Highly-graded beef was produced by dietary chromium methionine supplementation during fattening stage of Korean native steers. Body muscle was increased in replace of decreasing body fat in both pig and rat that were dietary supplemented with chromium methionine chelate. However, a pig farm study did not show any significant improvement of body gain upon supplementation of chromium methionine. Immune responses of pig and rat were not always dependent upon chromium form but were varied by species. These results suggest there could be a different mode of responses due to species as well as onset time of dietary supplementation of chromium methionine. It is still early to conclude the bio-efficacy of chromium methionine chelate presumably due to its recent appearance into the field. But the chelate is certainly worth more application to animal since it certainly reduced the application level of dietary chromium.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.7
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• pp.1202-1208
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• 2020

Objective: The aim of this study was to identify a distribution pattern of meat quality grade (MQG) as a function of carcass yield index (CYI) and the gender of Hanwoo (bull, cow, and steer) to determine the optimum point between both yield and quality. We also attempted to identify how pre- and post-deboning variables affect the gender-specific beef quality of Hanwoo. Methods: A total of 31 deboning variables, consisting of 7 pre-deboning and 24 post-deboning variables from bulls (n = 139), cows (n = 69), and steers (n = 153), were obtained from the National Institute of Animal Science (NIAS) in South Korea. The database was reconstructed to be suitable for a statistical significance test between the CYI and the MQG as well as classification of meat quality. Discriminant function analysis was used for classifying MQG using the deboning parameters of Hanwoo by gender. Results: The means of CYI according to 1+, 1, 2, and 3 of MQG were 68.64±2.02, 68.85±1.94, 68.62±5.88, and 70.99±3.32, respectively. High carcass yield correlated with low-quality grade, while high-quality meat most frequently was obtained from steers. The classification ability of pre-deboning parameters was higher than that of post-deboning parameters. Moisture and the shear force were the common significant parameters in all discriminant functions having a classification accuracy of 80.6%, 71%, and 56.9% for the bull, cow, and steer, respectively. Conclusion: This study provides basic information for predicting the meat quality by gender using pre-deboning variables consistent with the actual grading index.