• 제목/요약/키워드: Korean Native Cows

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Peri-estrus activity and mounting behavior and its application to estrus detection in Hanwoo (Korea Native Cattle)

  • Si Nae Cheon;Geun-Woo Park;Kyu-Hyun Park;Jung Hwan Jeon
    • Journal of Animal Science and Technology
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    • 제65권4호
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    • pp.748-758
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    • 2023
  • This study was conducted to investigate the change in activity and mounting behavior in Hanwoo (Korean Native Cattle) during the peri-estrus period and its application to estrus detection. A total of 20 Hanwoo cows were fitted with a neck-collar accelerometer device, which measured the location and acceleration of cow movements and recorded the number of instances of mounting behavior by the altitude data. The data were analyzed in three periods (24-, 6-, and 2-h periods). Blood samples were collected for 5 days after the prostaglandin F2α (PGF2α) injection, and the concentrations of estradiol, progesterone, follicle-stimulating hormone, and luteinizing hormone were determined by enzyme-linked immunosorbent assays. Activity and mounting behavior recorded over 2-h periods significantly increased as estrus approached and were more efficient at detecting estrus than over 24- and 6-h periods (p < 0.05). Endocrine patterns did not differ with the variation of individual cows during the peri-estrus period (p > 0.05). Activity was selected as the best predictor through stepwise discriminant analysis. However, activity alone is not enough to detect estrus. We suggest that a combination of activity and mounting behavior may improve estrus detection efficiency in Hanwoo. Further research is necessary to validate the findings on a larger sample size.

Developmental Competence of Oocytes Collected from Individual Ovaries of Slaughtered Korean Native Cattle with Grade of Meat Quality and Meat Yield

  • Kim, So-Seob;Kim, Ji-Sun;Park, Hum-Dae;Lee, Su-Kap;Park, Il-Kun;Lee, Dong-Won;Kim, Yun-Sik;Park, Yong-Su;Kim, Jae-Young;Park, Min-Chul;Lee, Jung-Hyung;Oh, Dae-Sik;Kim, Jae-Myeoung
    • 한국수정란이식학회지
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    • 제23권4호
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    • pp.291-298
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    • 2008
  • We separately cultured follicular oocytes collected from individual ovaries of slaughtered Korean native cows and examined both the embryonic development rate and pregnancy rate after embryo transplantation according to the meat yield and quality grades of the source beef carcass. Oocytes from meat yield grade B cows exhibited a higher fertilization rate and embryonic developmental rate to the eight-cell stage than oocytes from grade A or C animals (p<0.05), but there was no significant difference in rate of development to the blastocyst stage among meat yield grades A, Band C. The oocyte cleavage rate and development rate to the eight-cell stage from meat quality grade 3 cattle was higher than grades 1++, 1+, 1 and 2 (p<0.05). Embryos derived from grade animals displayed a development rate to the blastocyst stage of 19.4%, which was also higher than all other meat quality grades (p<0.05). Transplantation of in vitro-cultured oocytes from meat yield grade A ovaries led to a higher pregnancy rate (64.2%) than in vitro-cultured oocytes from meat yield grade B ovaries (56.5%), but there was no significant difference between the two groups in pregnancy or abortion rates. In conclusion, embryonic development rate and pregnancy rate has a close relation to meat quality grades of the source beef carcass, this results is to give information for the Korean native cows improvement of breed.

Prevalence of Theileria sergenti infection in Korean native cattle by polymerase chain reaction

  • Song, Kun-Ho;Sang, Byung-Chan
    • Parasites, Hosts and Diseases
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    • 제41권3호
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    • pp.141-145
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    • 2003
  • This study was performed to investigate the prevalence of theileriosis and to compare the prevalence of this disease in Korean native cattle reared under different environmental conditions, namely, in a grazing area and a non-grazing area by polymerase chain reaction. Three hundred and one Korean native cattle (276 cows and 25 bulls) that had not received prior treatment or been vaccinated to prevent theileriosis were examined by PCR for Theileria sergenti infection from 2001 to 2002. In our study, the parasitemia range in T. sergenti-positive cattle by microscopy were from 0.1 to 3% (mean 0.8%). In terms of mean prevalence, 204 of the 301 Korean native cattle (67.8%) were positive reaction by PCR. Our results also revealed that the infection rate among cows (70.3%) was significantly higher than that among bulls (40.0%) (p < 0.01). T. sergenti infection among the over 3 year-old-group (75%) had a significant higher prevalence than that among the less than 3 year-old-group (61.8%) (p < 0.05). Our data also showed that grazing areas (76.1%) had the significant higher prevalence than non-grazing areas (51%) (p < 0.001). In conclusion, this study demonstrates that the prevalence of T. sergenti infection is high and that its prevalence in grazing cattle is higher than that in non-grazing cattle, Therefore, life-long treatment and the development of an optimal vaccine are needed to reduce the numbers of bovine theileriosis in both grazing and non-grazing areas.

도축한우에서 난소낭종의 감별진단 및 기능성에 관한 연구 (Study on functional states and differential diagnosis of ovarian cysts in slaughtered Korean native cows)

  • 이청산;류대열;윤호규;송종한;이종인;서국현;김일화;강현구
    • 한국동물위생학회지
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    • 제27권1호
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    • pp.41-52
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    • 2004
  • To establish the differential diagnosis and functional status in ovarian cystic cows, progesterone(P$_4$) and estrogen(E$_2$) level of cystic follicular fluid, ultrasonography for measuring the cystic diameter and thickness of cystic wall, and histological findings were investigated in cystic ovaries from slaughtered Korean native cows. Ovarian follicles were classified as systic if the diameter was greater than 25 mm by ultrasonography. Ovarian cysts < 3 mm of cystic wall thickness, < 10 ng/ml P$_4$ concentration and >10 ng/ml E$_2$ concentration were classified follicular cyst, ovarian cysts 3 mm of cystic wall thickness, 10 ng/ml P$_4$ concentration and <10 ng/ml E$_2$ concentration were classified luteal cyst, and ovarian cysts 3 mm of cystic wall thickness, < 10 ng/ml P$_4$ concentration and <10 ng/ml E$_2$ concentration were classified non-functional ovarian cyst, respectively. Also ovarian cysts were classified 8 types by anatomical and hisctological findings. Ovarian cysts with corpus luteum were 3 of 73 cows and ovarian cysts without corpus luteum were 70 cows. The incidence rates of 8 various types of ovarian cysts were as follows; 2Aa 56.2%, 2Ba 20.5% and 2Ab 15.1%, respectively. The incidence rates of ovarian cysts without corpus luteum were follicular cyst 76.7% and luteal cyst 19.2%. The thickness of cystic wall were lAb 3.9 mm, 2Ab 3.3 mm and 2Bb 3.2 mm, and the cystic fluid P$_4$ concentrations were above 10.0 ng/ml in lAb, 2Ab and 2Bb, respectively. There was significantly correlations between the thickness of cystic wall and cystic fluid P$_4$ concentration in ovarian cysts(p<0.05). The ovarian cyst was classified follicular cysts, luteal cyst and non-functional ovarian cyst by hormone analysis. The luteal cyst was accuratly dignosed by cystic wall thickness. But follicular cysts was misdiagnosed 13 cows of 56 cystic cows. The 13 cystic cows was determined as had non-fuctional ovarian cysts. The cystic fluid P$_4$ concentration was 3.3 ng/ml in follicular ovarian cysts and 30.1 ng/ml luteinized ovarian cysts. There was significantly positive correlations between thickness of cystic wall and serum P$_4$ concentration in follicular(r$^2$ =0.59, p<0.001) and luteal cysts(r$^2$=0.65, p<0.001). These results indicated that ovarian cysts had various stages of degeneration and luteal cyst was accuratly diagnosed measurement of cystic wall thickness by ultrasonography, but follicular cysts was not diagnosed only cystic diameter and cystic wall thickness. In conclusion, it is suggest that ovarian cysts was diagnosed by combination of clinical sign and anatomical cystic features.

소에서 Coxiella burnetii의 검출 (Detection of Coxiella burnetii in Cattle)

  • 김요한;김두
    • 한국임상수의학회지
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    • 제32권6호
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    • pp.504-507
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    • 2015
  • Coxiella burnetii는 세포 내 기생하는 리케치아의 한 종류로 전세계에서 발병하는 인수공통 전염병인 큐열의 원인체이다. 국내에서 C. burnetii의 감염이 사람과 동물에서 보고되고 있지만 제한적인 지역에서만 실시되었다. 본 연구의 목적은 real-time PCR을 이용하여 소의 혈액과 유즙 시료에서 C. burnetii의 전국적인 검출률을 조사하는 것이다. 총 1,325 개 시료(한우 혈액 622 개, 젖소 혈액 185 개, 젖소 개체 유즙 170 개 및 젖소 집합유 348 개)를 전국적으로 채취하였다. 채취한 시료에서 Genomic DNA를 추출하였으며 C. burnetii 검출을 위한 real-time PCR을 실시하였다. 한우 혈액 622 개 중 17(2.7%) 개에서 C. burnetii가 검출되었고 젖소 혈액 185 개 중 2 (1.1%) 개에서 C. burnetii가 검출되었다. 젖소의 개체별 유즙 170 개 중 27(15.9%) 개에서 C. burnetii가 검출되었으며 집합유 348 개 중 84(24.1%) 개에서 C. burnetii가 검출되었다. 본 연구는 국내의 소가 C. burnetii에 높게 감염되어 있는 것을 확인하였으며, 인수공통질병인 큐열에 의한 가축과 사람의 피해를 예방하기 위하여, 가축과 사람의 큐열에 대한 추가적인 역학적 연구가 필요하다고 생각된다.

도축 한우에서 난소낭종의 기능성 평가 (Evaluation of Functional Status of Ovarian Cysts in Slaughtered Korean Native Cows)

  • 강현구;이청산;김일화;모인필;이기창;서국현
    • 한국수정란이식학회지
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    • 제21권1호
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    • pp.59-67
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    • 2006
  • 도축장에서 도축된 한우 중 난소낭종에 이환된 소 86두를 대상으로 수침법으로 초음파 검사를 실시하여 낭종의 직경, 낭종내강의 직경, 낭종벽의 두께를 측정하였으며 낭종액 중 $P_4$농도와 $E_2$농도를 측정하여 다음과 같은 결과를 얻었다. 난소낭종에 이환된 소 86두는 해부학적 및 초음파 검사에 의하여 단포성 난포낭종 51두(59.3%), 다포성 난포낭종 19두(22.1%), 단포성 황체낭종 13두(15.1%), 다포성 황체낭종 3두(3.5%)의 발생률을 나타내어 황체낭종보다 난포낭종의 발생률이 더 높았다. 해부학적 및 초음파 검사에 의해 분류된 난소낭종은 낭종액 중 호르몬 농도 측정에 의하여 기능성 난포낭종 54두, 기능성 황체낭종 16두, 비기능성 낭종 16두로 나타났으며 황체낭종에서는 모두 황체낭종으로 확인되었지만 난포낭종에서는 70두 중 16두가 기능을 하지 않은 낭종으로 확인되었다. 기능을 하지 않은 낭종을 가지고 있는 16두에서 단포성 낭종에서는 13두가, 다포성 낭종에서는 3두가 비기능성 낭종을 가지고 있었다. 난소낭종으로 진단된 예에서 낭종벽의 두께와 혈중 $P_4$ 농도 사이에는 유의성 있는 양의 상관 관계를 나타내었다(p<0.05). 또한 낭종벽의 두께와 낭종액 중 $P_4$ 농도와의 상관 관계는 난포낭종이 0.59(p<0.001), 황체낭종은 0.65(p<0.001), 비기능성 낭종은 0.52(p<0.06) 이었으며 난포낭종과 황체낭종 전체를 대상으로 하였을 때는 0.69로 유의성 있는 양의 상관관계를 나타내었다(p<0.001). 이상의 결과로 볼 때 난소낭종에는 그 변성 과정이 다양한 단계로 나누어지며 황체낭종은 초음파 검사에 의해 낭종벽의 두께를 측정함으로써 감별진단이 가능하지만 난포낭종은 낭종의 크기와 낭종벽의 두께만으로 감별 진단하는 데는 한계가 있음을 확인하였다. 따라서 난소낭종 특히 난포낭종의 진단에 있어서 단순히 형태학적인 소견에 의해 낭종을 진단하기보다는 임상증상과 더불어 종합적인 진단이 필요하다고 생각된다.

Changes in the ruminal fermentation and bacterial community structure by a sudden change to a high-concentrate diet in Korean domestic ruminants

  • Lee, Mingyung;Jeong, Sinyong;Seo, Jakyeom;Seo, Seongwon
    • Asian-Australasian Journal of Animal Sciences
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    • 제32권1호
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    • pp.92-102
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    • 2019
  • Objective: To investigate changes in rumen fermentation characteristics and bacterial community by a sudden change to a high concentrate diet (HC) in Korean domestic ruminants. Methods: Major Korean domestic ruminants (each of four Hanwoo cows; $545.5{\pm}33.6kg$, Holstein cows; $516.3{\pm}42.7kg$, and Korean native goats; $19.1{\pm}1.4kg$) were used in this experiment. They were housed individually and were fed ad libitum with a same TMR (800 g/kg timothy hay and 200 g/kg concentrate mix) twice daily. After two-week feeding, only the concentrate mix was offered for one week in order to induce rapid rumen acidosis. The rumen fluid was collected from each animals twice (on week 2 and week 3) at 2 h after morning feeding using an oral stomach tube. Each collected rumen fluid was analyzed for pH, volatile fatty acid (VFA), and $NH_3-N$. In addition, differences in microbial community among ruminant species and between normal and an acidosis condition were assessed using two culture-independent 16S polymerase chain reaction (PCR)-based techniques (terminal restriction fragment length polymorphism and quantitative real-time PCR). Results: The HC decreased ruminal pH and altered relative concentrations of ruminal VFA (p<0.01). Total VFA concentration increased in Holstein cows only (p<0.01). Terminal restriction fragment length polymorphism and real-time quantitative PCR analysis using culture-independent 16S PCR-based techniques, revealed rumen bacterial diversity differed by species but not by HC (p<0.01); bacterial diversity was higher in Korean native goats than that in Holstein cows. HC changed the relative populations of rumen bacterial species. Specifically, the abundance of Fibrobacter succinogenes was decreased while Lactobacillus spp. and Megasphaera elsdenii were increased (p<0.01). Conclusion: The HC altered the relative populations, but not diversity, of the ruminal bacterial community, which differed by ruminant species.

Analysis of Placental Proteins in Somatic Cell Clone Recipient Cows

  • Woo, Jei-Hyun;Chung, Hak-Jae;Kim, Bong-Ki;Ko, Yeoung-Gyu;Kim, Jeom-Soon;Jung, Jin-Kwan;Chang, Won-Kyong
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2004년도 춘계학술발표대회
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    • pp.197-197
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    • 2004
  • The purpose of this experiment was to investigate the protein profiles in the placenta of Korean native cows(KNC) transferred cloned embryos and KNC artificially inseminated placental tissues were collected from the cows after cesarean section around parturition, and placental proteins were analyzed. Using two dimensional polyacrylamide gel eletrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. (omitted)

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Culture Conditions for In Vitro Maturation of Abattoir Derived Oocytes of Native Zebu Cows of Bangladesh

  • Morshed, S.M. Niyaz;Bhuiyan, Mohammad Musharraf Uddin;Rahman, Mohammad Moshiur;Singha, Joydev Kumer;Juyena, Nasrin Sultana
    • 한국수정란이식학회지
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    • 제29권3호
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    • pp.201-206
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    • 2014
  • The objectives of the study were to determine an effective culture dish, culture duration and protein supplementation in medium for in vitro maturation (IVM) of oocytes of native zebu cows in Bangladesh. The ovaries of cows were collected from local slaughterhouse followed by aspiration of follicular fluid. The cumulus-oocyte-complexes (COCs) with more than 3 compact cumulus cell layers were cultured in tissue culture medium (TCM) 199 for maturation. The maturation of oocytes was determined by observing polar body under microscope. To determine an effective culture dish, 130 COCs derived from 48 ovaries in a well of 4-well dish and 102 COCs derived from 36 ovaries in drops covered with mineral oil within 35 mm petri dish were cultured for 24 hours. The rate of maturation of oocytes did not vary between 4-well dish ($51.3{\pm}15.0%$) and drops in petri dish ($52.4{\pm}11.6%$). To determine the effective culture duration, 185 COCs derived from 62 ovaries were cultured in drops for 18, 21, 24 and 27 hours. The rate of maturation of occytes ranged from $51.9{\pm}9.4%$ (18 hours) to $59.0{\pm}17.1%$ (27 hours) and the difference in maturation rate among different culture durations was not significant (P>0.05). To determine an effective protein supplementation, 63 oocytes from 19 ovaries were cultured separately in TCM 199 supplemented with either fetal bovine serum (FBS) or bovine serum albumin (BSA). The rate of maturation was significantly (P<0.01) higher in medium supplemented with FBS ($55.63{\pm}16.19%$) than that of BSA ($14.82{\pm}9.36%$). In conclusion, COCs of native zebu cows can be cultured for IVM either in 4-well culture dish or droplets in petri dish for 18 to 27 hours in medium supplemented with FBS.

제주지역 한우의 소 바이러스성 설사병 바이러스 감염실태 (Prevalence of bovine viral diarrhea virus from Korean native cattle farms in Jeju)

  • 조성철;양형석;박창남;김시택;고은주;손원근
    • 대한수의학회지
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    • 제63권2호
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    • pp.12.1-12.7
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    • 2023
  • Bovine viral diarrhea virus (BVDV) is an RNA virus belonging to Pestivirus in the family Flaviviridae. BVDV has economic significance for the livestock industry because of its association with acute disease, fetal loss, and birth of persistently infected (PI) animals. This study aimed to investigate the BVDV infection rates in Korean native cattle farms in Jeju for further planning of a BVDV control program in the Jeju Province. BVDV antibodies and antigens were tested in 15,842 sera collected from 302 Korean native cattle herds between January 2014 and June 2017 using enzyme-linked immunosorbent assay (ELISA). Viral antigen was detected by reverse transcription-polymerase chain reaction from 60 sera that were antigen ELISA-positive. BVDV antibodies were found in 90.7% (274/302) herds and 61.1% (9,678/15,842) cows. BVDV antigens were found in 13.2% (40/302) herds and 0.4% (61/15,842) cows. The oldest animal group (> 8 years) exhibited the highest sero-positive rates (91%), while the youngest animal group (< 1 years) had the highest antigen positivity rates (0.52%). Of the 60 antigen-positive sera, BVDV types 1 and 2 were found in 36 and 12 sera, respectively. Additionally, six animals were considered to be PI as BVDV was continually detected in annual examination.