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http://dx.doi.org/10.17555/jvc.2015.12.32.6.504

Detection of Coxiella burnetii in Cattle  

Kim, Yo-Han (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Kangwon National University)
Kim, Doo (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Kangwon National University)
Publication Information
Journal of Veterinary Clinics / v.32, no.6, 2015 , pp. 504-507 More about this Journal
Abstract
Coxiella burnetii is an obligate intracellular rickettsial organism and the causative agent of Query fever, a zoonosis that occurs worldwide. In Korea, C. burnetii infection had occurred in humans and animals. However, the studies were only conducted in geographically limited area for detection of C. burnetii. The objective of this study was to detect C. burnetii in Korean native cattle and dairy cattle nationwide by real-time PCR. The total of 807 blood samples from 622 Korean native cattle and 185 dairy cows, 170 individual milk samples of dairy cows, and 348 bulk tank milk samples of dairy herds were collected nationwide. From blood samples, C. burnetii was detected in 17 (2.7%) out of 622 Korean native cattle and 2 (1.1%) of 185 dairy cows. From milk samples, C. burnetii was detected in 27 (15.9%) out of 170 individual milk samples of dairy cows. And C. burnetii was detected in 84 (24.1%) of 348 bulk tank milk samples. In conclusion, this study revealed that the detection rates are considerably high in cattle and the infection of C. burnetii has been continuously occurring in cattle of Korea. In order to prevent the hazards of a zoonosis Q-fever that occur both humans and domestic animals, further studies are needed to clarify the epidemiology of Q-fever of domestic animals and humans in Korea.
Keywords
Coxiella burnetii; real-time PCR; Korean native cattle; dairy cattle;
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1 Abinanti FR, Welsh HH, Lennette EH, Brunetti O. Q-fever studies. XVI. Some aspects of the experimental infection induced in sheep by the intratracheal route of inoculation. Am J Hyg 1953; 57: 170-184.
2 Arricau BN, Rodolakis A. Is Q-fever an emerging or reemerging zoonosis? Vet Res 2005; 36: 327-349.   DOI
3 Babudieri B. Q-fever: a zoonosis. Adv Vet Sci 1959; 5: 81.
4 Barlow J, Rauch B, Welcome F, Kim SG, Dubovi E, Schukken Y. Association between Coxiella burnetii shedding in milk and subclinical mastitis in dairy cattle. Vet Res 2008; 39: 23.   DOI
5 Berri M, Souriau A, Crosby M, Crochet D, Lechopier P, Rodolakis A. Relationships between the shedding of Coxiella burnetii, clinical signs and serological responses of 34 sheep. Vet Rec 2001; 148: 502-505.   DOI
6 CDC. Code of Hygienic Practice for Milk and Milk Products. CAC/RCP 57-2004. Atlanta, GA, USA, 2004.
7 Cerf O, Condron R. Coxiella burnetii and milk pasteurization: an early application of the precautionary principle? Epidemiol Infect 2006; 134: 946-951.   DOI
8 Cho SN, Baek SH, Chong YS, Kim JD, Lee WY. Prevalence of antibodies to the Coxiella burnetii Phase II antigen among residents in Korea. J Korean Soc Microbiol 1993; 28: 223-228.
9 de Bruin A, van der Plaats RQ, de Heer L, Paauwe R, Schimmer B, Vellema P, van Rotterdam BJ, van Duynhoven YT. Detection of Coxiella burnetii DNA on small-ruminant farms during a Q-fever outbreak in the Netherlands. Appl Environ Microbiol 2012; 78: 1652-1657.   DOI
10 Denison AM, Thompson HA, Massung RF. IS1111 insertion sequences of Coxiella burnetii: characterization and use for repetitive element PCR-based differentiation of Coxiella burnetii isolates. BMC Microbiol 2007; 7: 91.   DOI
11 Fishbein DB, Raoult D. A cluster of Coxiella burnetii infections associated with exposure to vaccinated goats and their unpasteurized dairy products. Am J Trop Med Hyg 1992; 47: 35-40.   DOI
12 Guatteo R, Beaudeau F, Joly A, Seegers H. Coxiella burnetii shedding by dairy cows. Vet Res 2007; 38: 849-860.   DOI
13 Gyuranecz M, Denes B, Hornok S, Kovacs P, Horvath G, Jurkovich V, Varga T, Hajtos I, Szabo R, Magyar T, Vass N, Hofmann-Lehmann R, Erdelyi K, Bhide M, Dan A. Prevalence of Coxiella burnetii in Hungary: screening of dairy cows, sheep, commercial milk samples, and ticks. Vector Borne Zoonotic Dis 2012; 12: 650-653.   DOI
14 Htwe KK, Amano K, Sugiyama Y, Yagami K, Minamoto N, Hashimoto A, Yamaguchi T, Fukushi H, Hirai K. Seroepidemiology of Coxiella burnetii in domestic and companion animals in Japan. Vet Rec 1992; 131: 490.   DOI
15 Marrie TJ. Epidemiology of Q-fever. In, Q-fever. Boston: CRC Press Inc. 1990: 49-70.
16 Kim SG, Kim EH, Lafferty CJ, Dubovi E. Coxiella burnetii in Bulk Tank Milk Samples, United States. Emerg Infec Dis 2005; 11: 619-621.   DOI
17 Kim WJ, Hahn TW, Kim DY, Lee MG, Jung KS, Ogawa M, Kishimoto T, Lee ME, Lee SJ. Seroprevalence of Coxiella burnetii infection in dairy cattle and non-symptomatic people for routine health screening in Korea. J Korean Med Sci 2006; 21: 823-826.   DOI
18 Klee SR, Tyczka J, Ellerbrok H, Franz T, Linke S, Baljer G, Appel B. Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii. BMC Microbiol 2006; 6: 2.   DOI
19 Maurin M, Raoult D. Q-fever. Clin Microbiol Rev 1999; 12: 518-553.
20 Muskens J, van Engelen E, van Maanen C, Bartels C, Lam TJ. Prevalence of Coxiella burnetii infection in Dutch dairy herds based on testing bulk tank milk and individual samples by PCR and ELISA. Vet Rec 2011; 168: 79.   DOI
21 Park MS, Park MY, Shin YO. Distributions of antibodies to Coxiella burnetii in Patients with Unknown Fever and Atypical Pneumonia. J Bacteriol Viol 2003; 33: 307-315.
22 Pierre EF, Thomas JM, Didier R. Diagnosis of Q Fever. J Clin Microbiol 1998; 36: 1823-1834.
23 Seshadri R, Paulsen IT, Eisen JA, Read TD, Nelson KE, Nelson WC, Ward NL, Tettelin H, Davidsen TM, Beanan MJ, Deboy RT, Daugherty SC, Brinkac LM, Madupu R, Dodson RJ, Khouri HM, Lee KH, Carty HA, Scanlan D, Heinzen RA, Thompson HA, Samuel JE, Fraser CM, Heidelberg JF. Complete genome sequence of the Q-fever pathogen Coxiella burnetii. Proc Natl Acad Sci USA 2003; 100: 5455-5460.   DOI
24 van Loenhout JA, Paget WJ, Vercoulen JH, Wijkmans CJ, Hautvast JL, van der Velden K. Assessing the long-term health impact of Q-fever in the Netherlands: a prospective cohort study started in 2007 on the largest documented Qfever outbreak to date. BMC Infect Dis 2012; 12: 280.   DOI
25 Sobradillo V, Ansola P, Baranda F, Corral C. Q-fever pneumonia: a review of 164 community-acquired cases in the Basque country. Eur Respir J 1989; 2: 263-266.
26 Tsironi M, Andriopoulos P, Fokas S. Acute Q-fever lobar pneumonia: a case report. J Infect 2005; 51: 89-91.   DOI
27 Vaidya VM, Malik SVS, Kaur S, Kumar S, Barbuddhe SB. Comparison of PCR, Immunofluorescence Assay, and Pathogen Isolation for Diagnosis of Q-fever in Humans with Spontaneous Abortions. J Clin Microbiol 2008; 46: 2038-2044.   DOI
28 Waag DM, England MJ, Tammariello RF, Byrne WR, Gibbs P, Banfield CM, Pitt ML. Comparative efficacy and immunogenicity of Q fever chloroform:methanol residue (CMR) and phase I cellular (Q-Vax) vaccines in cynomolgus monkeys challenged by aerosol. Vaccine 2002; 20: 2623-2634.   DOI
29 Willems HTD, Frolich RR, Krauss H. Detection of Coxiella burnetii in cow's milk using the polymerase chain reaction (PCR). Zentralbl Veterinarmed B 1994; 41: 580-587.