Browse > Article
http://dx.doi.org/10.5713/ajas.18.0262

Changes in the ruminal fermentation and bacterial community structure by a sudden change to a high-concentrate diet in Korean domestic ruminants  

Lee, Mingyung (Division of Animal and Dairy Sciences, Chungnam National University)
Jeong, Sinyong (Division of Animal and Dairy Sciences, Chungnam National University)
Seo, Jakyeom (Division of Animal and Dairy Sciences, Chungnam National University)
Seo, Seongwon (Division of Animal and Dairy Sciences, Chungnam National University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.32, no.1, 2019 , pp. 92-102 More about this Journal
Abstract
Objective: To investigate changes in rumen fermentation characteristics and bacterial community by a sudden change to a high concentrate diet (HC) in Korean domestic ruminants. Methods: Major Korean domestic ruminants (each of four Hanwoo cows; $545.5{\pm}33.6kg$, Holstein cows; $516.3{\pm}42.7kg$, and Korean native goats; $19.1{\pm}1.4kg$) were used in this experiment. They were housed individually and were fed ad libitum with a same TMR (800 g/kg timothy hay and 200 g/kg concentrate mix) twice daily. After two-week feeding, only the concentrate mix was offered for one week in order to induce rapid rumen acidosis. The rumen fluid was collected from each animals twice (on week 2 and week 3) at 2 h after morning feeding using an oral stomach tube. Each collected rumen fluid was analyzed for pH, volatile fatty acid (VFA), and $NH_3-N$. In addition, differences in microbial community among ruminant species and between normal and an acidosis condition were assessed using two culture-independent 16S polymerase chain reaction (PCR)-based techniques (terminal restriction fragment length polymorphism and quantitative real-time PCR). Results: The HC decreased ruminal pH and altered relative concentrations of ruminal VFA (p<0.01). Total VFA concentration increased in Holstein cows only (p<0.01). Terminal restriction fragment length polymorphism and real-time quantitative PCR analysis using culture-independent 16S PCR-based techniques, revealed rumen bacterial diversity differed by species but not by HC (p<0.01); bacterial diversity was higher in Korean native goats than that in Holstein cows. HC changed the relative populations of rumen bacterial species. Specifically, the abundance of Fibrobacter succinogenes was decreased while Lactobacillus spp. and Megasphaera elsdenii were increased (p<0.01). Conclusion: The HC altered the relative populations, but not diversity, of the ruminal bacterial community, which differed by ruminant species.
Keywords
High Concentrate Diet; Korean Domestic Ruminants; Fermentation Characteristics; Bacterial Diversity; Relative Population;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Lettat A, Nozière P, Silberberg M, et al. Rumen microbial and fermentation characteristics are affected differently by bacterial probiotic supplementation during induced lactic and subacute acidosis in sheep. BMC Microbiol 2012;12:142.   DOI
2 Tajima K, Aminov RI, Nagamine T, et al. Diet-dependent shifts in the bacterial population of the rumen revealed with real-time PCR. Appl Environ Microbiol 2001;67:2766-74.   DOI
3 Lee HJ, Jung JY, Oh YK, et al. Comparative survey of rumen microbial communities and metabolites across one caprine and three bovine groups, using bar-coded pyrosequencing and 1H nuclear magnetic resonance spectroscopy. Appl Environ Microbiol 2012;78:5983-93.   DOI
4 Duffield T, Plaizier JC, Fairfield A, et al. Comparison of techniques for measurement of rumen pH in lactating dairy cows. J Dairy Sci 2004;87:59-66.   DOI
5 Erwin E, Marco G, Emery E. Volatile fatty acid analyses of blood and rumen fluid by gas chromatography. J Dairy Sci 1961;44: 1768-71.   DOI
6 Chaney AL, Marbach EP. Modified reagents for determination of urea and ammonia. Clin Chem 1962;8:130-2.   DOI
7 Rius AG, Kittelmann S, Macdonald KA, et al. Nitrogen metabolism and rumen microbial enumeration in lactating cows with divergent residual feed intake fed high-digestibility pasture. J Dairy Sci 2012;95:5024-34.   DOI
8 Abdo Z, Schüette UM, Bent SJ, et al. Statistical methods for characterizing diversity of microbial communities by analysis of terminal restriction fragment length polymorphisms of 16S rRNA genes. Environ Microbiol 2006;8:929-38.   DOI
9 Denman SE, McSweeney CS. Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen. FEMS Microbiol Ecol 2006;58: 572-82.   DOI
10 Nie Y, Zhou Z, Guan J, et al. Dynamic changes of yak (Bos grunniens) gut microbiota during growth revealed by polymerase chain reaction-denaturing gradient gel electrophoresis and metagenomics. Asian-Australas J Anim Sci 2017;30:957-66.   DOI
11 Zhan J, Liu M, Wu C, et al. Effects of alfalfa flavonoids extract on the microbial flora of dairy cow rumen. Asian-Australas J Anim Sci 2017;30:1261-9.   DOI
12 Tajima K, Arai S, Ogata K, et al. Rumen bacterial community transition during adaptation to high-grain diet. Anaerobe 2000;6:273-84.   DOI
13 Plaizier JC. Replacing chopped alfalfa hay with alfalfa silage in barley grain and alfalfa-based total mixed rations for lactating dairy cows. J Dairy Sci 2004;87:2495-505.   DOI
14 Owens FN, Secrist DS, Hill WJ, Gill DR. Acidosis in cattle: a review. J Anim Sci 1998;76:275-86.   DOI
15 Burrin D, Britton R. Response to monensin in cattle during subacute acidosis. J Anim Sci 1986;63:888-93.   DOI
16 Khafipour E, Li S, Plaizier JC, Krause DO. Rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis. Appl Environ Microbiol 2009;75: 7115-24.   DOI
17 In SH, Shin HT, Baig SY, Chung KH. Effects of lasalocid supplementation on the ruminal fermentation and digestibility of Korean native goats. J Anim Sci Technol 2001;43:101-10.
18 Bryant MP. Bacterial species of the rumen. Bacteriol Rev 1959; 23:125-53.   DOI
19 Ye H, Liu J, Feng P, Zhu W, Mao S. Grain-rich diets altered the colonic fermentation and mucosa-associated bacterial communities and induced mucosal injuries in goats. Sci Rep 2016;6: 20329.   DOI
20 Whitford MF, Forster RJ, Beard CE, Gong J, Teather RM. Phylogenetic analysis of rumen bacteria by comparative sequence analysis of cloned 16S rRNA genes$\ss$. Anaerobe 1998;4:153-63.   DOI
21 Chiquette J, Allison M, Rasmussen M. Use of Prevotella bryantii 25A and a commercial probiotic during subacute acidosis challenge in midlactation dairy cows. J Dairy Sci 2012;95:5985-95.   DOI
22 Liu K, Xu Q, Wang L, et al. The impact of diet on the composition and relative abundance of rumen microbes in goat. Asian-Australas J Anim Sci 2017;30:531-7.   DOI
23 Freeman WM, Walker SJ, Vrana KE. Quantitative RT-PCR: pitfalls and potential. BioTechniques 1999;26:112-25.   DOI
24 Koike S, Kobayashi Y. Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens. FEMS Microbiol Lett 2001;204:361-6.   DOI
25 Petri RM, Schwaiger T, Penner GB, et al. Characterization of the core rumen microbiome in cattle during transition from forage to concentrate as well as during and after an acidotic challenge. PloS ONE 2013;8: e83424.   DOI
26 Mackie RI, Gilchrist F, Robberts AM, Hannah PE, Schwartz HM. Microbiological and chemical changes in the rumen during the stepwise adaptation of sheep to high concentrate diets. J Agric Sci 1978;90:241-54.   DOI
27 Allison MJ, Robinson IM, Dougherty RW, Bucklin JA. Grain overload in cattle and sheep: changes in microbial populations in the cecum and rumen. Am J Vet Res 1975;36:181-5.
28 Nagaraja TG, Titgemeyer EC. Ruminal acidosis in beef cattle: the current microbiological and nutritional outlook. J Dairy Sci 2007;90:E17-38.   DOI
29 Huber TL, Cooley JH, Goetsch DD, Das NK. Lactic acid-utilizing bacteria in ruminal fluid of a steer adapted from hay feeding to a high-grain ration. Am J Vet Res 1976;37:611-3.
30 Counotte GHM, Prins RA. Regulation of lactate metabolism in the rumen. Vet Res Commun 1981;5:101-15.   DOI
31 Goad DW, Goad CL, Nagaraja TG. Ruminal microbial and fermentative changes associated with experimentally induced subacute acidosis in steers. J Anim Sci 1998;76:234-41.   DOI
32 Hungate RE. The rumen and its microbes. New York, USA: Academic Press; 1966.
33 Russell JB. Rumen microbiology and its role in ruminant nutrition. Ithaca, NY, USA: J.B. Russell Publishing Co.; 2002.
34 Mackie R, Gilchrist FM. Changes in lactate-producing and lactate-utilizing bacteria in relation to pH in the rumen of sheep during stepwise adaptation to a high-concentrate diet. Appl Environ Microbiol 1979;38:422-30.   DOI
35 Lean IJ, Wade LK, Curtis MA, Porter J. New approaches to control of ruminal acidosis in dairy cattle. Asian-Australas J Anim 2000;13(Suppl):266-9.
36 Henderson G, Cox F, Ganesh S, et al. Rumen microbial community composition varies with diet and host, but a core microbiome is found across a wide geographical range. Sci Rep 2015;5:14567.   DOI
37 Fernando SC, Purvis H, Najar F, et al. Rumen microbial population dynamics during adaptation to a high-grain diet. Appl Environ Microbiol 2010;76:7482-90.   DOI
38 Therion JJ, Kistner A, Kornelius JH. Effect of pH on growth rates of rumen amylolytic and lactilytic bacteria. Appl Environ Microbiol 1982;44:428-34.   DOI
39 Malekkhahi M, Tahmasbi AM, Naserian AA, et al. Effects of supplementation of active dried yeast and malate during sub-acute ruminal acidosis on rumen fermentation, microbial population, selected blood metabolites, and milk production in dairy cows. Anim Feed Sci Technol 2016;213:29-43.   DOI
40 McCann JC, Luan S, Cardoso FC, et al. Induction of subacute ruminal acidosis affects the ruminal microbiome and epithelium. Front Microbiol 2016;7:701.
41 Russell JB, Baldwin RL. Substrate preferences in rumen bacteria: evidence of catabolite regulatory mechanisms. Appl Environ Microbiol 1978;36:319-29.   DOI
42 Ouwerkerk D, Klieve AV, Forster RJ. Enumeration of Megasphaera elsdenii in rumen contents by real‐time Taq nuclease assay. J Appl Microbiol 2002;92:753-8.   DOI
43 Huo W, Zhu W, Mao S. Impact of subacute ruminal acidosis on the diversity of liquid and solid-associated bacteria in the rumen of goats. World J Microbiol Biotechnol 2014;30:669-80.   DOI
44 Stevenson DM, Weimer PJ. Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR. Appl Microbiol Biotechnol 2007;75:165-74.   DOI
45 Lan Y, Xun S, Tamminga S, et al. Real-time PCR detection of lactic acid bacteria in cecal contents of eimeria tenella-lnfected broilers fed soybean oligosaccharides and soluble soybean polysaccharides. Poult Sci 2004;83:1696-702.   DOI