• 한국초지조사료학회지
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• 제4권3호
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• pp.180-186
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• 1984

Timothy-Orchardgrass우점(優点) 초지(草地)에서 한우(韓牛) 및 교잡우(交雜牛)를 방목축(放牧畜)으로 공시(供試)하여 1980년(年) 6월(月)부터 1982년(年) 10월(月)까지 3년간(年間) 방목(放牧) 방법간(方法間)에 증체량 건물생산량(乾物生産量), 방목습성등(放牧習性等)의 차이(差異)를 검토(檢討)코자 시험(試驗)을 실시(實施)하여 얻어진 결과(結果)는 아래와 같다. 1. ha당(當) 목초건물수량(牧草乾物收量)은 'Wye 대학(大學)' 방목(放牧)이 6936.7kg, 고정방목(固定放牧)이 6794.3kg 수정고정방목(修正固定放牧)이 6686.9kg 윤환방목(輪換放牧)이 6675.7kg 그리고 예취급여구(刈取給與區)가 5083.4kg으로 방목방법간(放牧方法間)에는 유의성(有意性)이 없었다. 2 목초이용율(牧草利用率)은 예취급여구(刈取給與區)가 89.3%로 가장 높았고 윤환방목(輪換放牧), Wye 대학방목(大學放牧), 수정고정방목(修正固定放牧) 그리고 고정방목순(固定放牧順)으로 각각 73.1%, 70.8%, 64.4% 및 46.1%로 처리간(處理間)에 높은 유의성(有意性)(P<0.01)이 나타났으며 3. 1일(日) 두당건물(頭當乾物) 채식량(採食量)은 Wye 대학방목(大學放牧)이 7.32kg 윤환방목(輪換放牧)이 6.45kg 고정방목(固定放牧)이 5.82kg 그리고 예취급여구(刈取給與區)가 5.61kg순(順)으로 Wye대학(大學) 방목(放牧)이 가장 많았고 예취급여구(刈取給與區)가 가장 적었다. 4. 일(日) 당증체량을 보면 Wye 대학(大學) 방목(放牧)이 0.60kg으로 가장 높았고 윤환방목(輪換放牧)이 0.55kg 수정고정방목(修正固定放牧)이 0.5kg 고정방목(固定放牧)이 0.43kg 그리고 예취급여구(刈取給與區) 0.42kg으로 처리간(處理間)에는 유의성(有意性)이 없었다. 5. ha당(當) 총증체량에 있어서 Wye 대학방목(大學放牧)이 33339.5kg으로 가장 많았고 윤환방목(輪換放牧), 수정고정방목(修正固定放牧), 예취급여구(刈取給與區) 그리고 고정방목순(固定放牧順)으로 각각 299.1kg, 253.3kg, 226.6kg 및 222.2kg으로 고정방목구(固定放牧區)가 가장 적었다. 6. 채식시기(採食時期)은 각(各) 방목방법간(放牧方法間)에 유의차(有意差)는 나타나지 않았지만 Wye 대학(大學) 방목구(放牧區)가 367.3 분(分)으로 가장 짧았고 고정방목구(固定放牧區)가 422.1분(分)으로 가장 길었다. 7. 계절별(季節別) 1일(日) 채식시간(採食時間)은 6월(月)이 304.9분(分) 7월(月)이 359.4분(分) 8월(月)에 437.9분(分)이 483.3분(分) 그리고 10월(月)이 395.8분(分)으로 계절간(季節間)에 유의차(有意差)(P<0.05)가 나타났으며 계절(季節)이 진행(進行)할수록 채식시간(採食時間)이 길어진 것으로 나타났다. 8. 품종별(品種別) 채식시간(採食時間)에는 차이(差異)가 없었으며 샤로레${\times}$한우(韓牛)가 457.0분(分)으로 가장 길었고 한우(韓牛)가 422.4분(分)으로 가장 짧았다.

• 한국동물위생학회지
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• 제42권2호
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• pp.117-120
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• 2019

Bovine viral diarrhea Virus (BVDV) infections cause respiratory, gastrointestinal, and reproductive problems, such as infertility, abortion, stillbirth, and sickly offspring. Many countries have reduced the economic damage through the application of different control programmes, and some have successfully eradicated BVD. Detection and elimination of cattle persistently infected (PI) with BVDV is important for BVD eradication because PI cattle are a main source of BVD transmission. In this study, the prevalence of Korean native cattle persistently infected (PI) with BVDV was investigated and determined in 49 farms with 3,050 cattle. The all samples were collected by ear notch sampling. Korean native cattle with initial positives on antigen-ELISA (Ag-ELISA) were sampled again after 3~4 weeks and cattle with second positives in both Ag-ELISA and immunohistochemistry (IHC) were identified as PI cattle. Among the 49 farms, 14 (28.6%) farms had at least more than one PI cow and 21 (0.69%) of 3,050 cattle were determined as PI cattle. As a result of this work, it is suggested that national BVD eradication program is required to reduce economic losses by BVDV infection in Korean cattle industries.

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2000년도 춘계심포지움
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• pp.64-75
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• 2000

During the last three decades considerable advances has been made in goat embryo production and transfer technology. The Korean native black goat is the most useful domestic ruminant in this country for biological investigation and application because it has a lot of merits such as relatively short generation period (1 vs 2 year for a cow), easy of handling, well adaptation, high fertility, convenient and inexpensive. This article covers the methods of superovulation, estrus synchronization, embryo collection and transfer techniques, pregnancy diagnosis and subsequent pregnancy and kidding rates for the production of transgenic Korean native black goats. More than one hundred goat kids have been produced as a result of our transgenic goat project via microinjection of foreign gene into pronuclei, in vitro culture, transfer of various stages of fresh and frozen-thawed microinjected embryos into oviducts or uteri of recipient does. We have got two transgenic goats carrying a transgene targeting the expression of recombinant human granulocyte colony stimulating factor (hG-CSF) to the mammary gland so far. Since collection and transfer of embryos in this species is usually accomplished by laparotomy, exteriorization of the reproductive tract for surgical embryo collection leads to the formation of post-operative adhesions. Nonsurgical or laparoscopic technique to reduce adhesions from repeated surgeries has great advantages in improving embryo production and transfer especially from valuable donors. We will discuss this later.

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2000년도 수정란의 위생적 처리·검사 및 특수가축의 수정란이식 기술 심포지움
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• pp.64-75
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• 2000

During the last three decades considerable advances has been made in goat embryo production and transfer technology. The Korean native black goat is the most useful domestic ruminant in this country for biological investigation and application because it has a lot of merits such as relatively short generation period(1 vs 2 year for a cow), easy of handling, well adaptation, high fertility, convenient and inexpensive. This article covers the methods of superovulation, estrus synchronization, embryo collection and transfer techniques, pregnancy diagnosis and subsequent pregnancy and kidding rates for the production of transgenic Korean native black goats. More than one hundred goat kids have been produced as a result of our transgenic goat project via microinjection of foreign gene into pronuclei, in vitro culture, transfer of various stages of fresh and frozen-thawed microinjected embryos into oviducts or uteri of recipient does. We have got two transgenic goats carrying a transgene targeting the expression of recombinant human granulocyte colony stimulating factor(hG-CSF) to the mammary gland so far. Since collection and transfer of embryos in this species is usually accomplished by laparotomy, exteriorization of the reproductive tract for surgical embryo collection leads to the formation of post-operative adhesions. Nonsurgical or laparoscopic technique to reduce adhesions from repeated surgeries has great advantages in improving embryo production and transfer especially from valuable donors. We will discuss this later.

• 대한수의학회지
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• 제30권2호
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• pp.243-247
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• 1990

A study was conducted to improve the reproductive performance of Korean native cows. Plasma progesterone concentrations were determined by radioimmunoassay during the estrous cycle and early pregnancy. Plasma progesterone concentrations during the estrous cycle in the 15 cows were the lowest (0.30ng/ml) at estrus, gradually increased after 6 days, remained high from 8 to 17 days (3.87~5.59ng/ml) and drastically decreased thereafter to reach minimal concentrations at the next estrus. Plasma progesterone concentrations of the 10 pregnant cows at 20 days after insemination were $5.82{\pm}1.33ng/ml$ (mean${\pm}$SD) and those of 10 non-pregnant cows were $0.30{\pm}0.21ng/ml$, respectively. Therefore, plasma progesterone concentrations in the pregnant cows at 20 days after insemination were significantly higher than in non-pregnant cows (p<0.001).

• 한국수정란이식학회지
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• 제12권2호
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• pp.203-209
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• 1997

This study was carried out to compare the actual size(length and height) of ovaries, follicles and corpora lutea of Korean native cow with those on sonograms. We used 3 different probes(3.5 MHz abdominal probe, 6.5 MHz transvaginal probe and 5.0 MHz transrectal probe) and a calipher for measurements of ovaries, follicles and corpora lutea on sonograms and actual size. Under water immersion, 157 ovaries were scanned with 3 probes and measured in actual size and compared each other. The average height and width of ovaries of Korean native cows were 17.40$\pm$3.99 and 34.23$\pm$6.02mm, respectively. In comparison of height, length of ovaries and preovulation follicles, we found that image with a transvaginal probe was nearly the same as the actual size(p<0.01), but with an abdominal probe the image was appeared larger than the actual size. In measurement(diameter) of preovulation follicles the transvaginal probe was proven to be more accurate to the actual size than other probes and in corpus luteum measurement all probes were accurate. In the comparison of number of follicles by different size ranges, there was no statistical difference in the count of follicles over 10 mm in diameter between the transvaginal probe and naked eyes.

• 한국임상수의학회지
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• 제21권3호
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• pp.280-285
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• 2004

Pathological and bacteriological studies were made of a total of 111 udders from slaughtered Korean native cows originating from various locations in Chonnam province. Incidence of supernumerary teats was 8.1%. Gross lesions were found in 21.4% of mucous membranes of total teat cisterns; these lesions comprised epithelial hyperplasia (12.6%), congestion (4.1%), slight fibrosis (3.8%) and severe fibrosis with congestion (0.9%). Gross lesions in the glandular tissues were found in 35.1% of cows; these lesions comprised slight fibrosis (27.9%), moderate fibrosis (6.3%) and severe fibrosis with pus (0.9%). Mastitis was found only in one cow. One hundred and twenty-five strains of microorganisms were isolated from mammary gland fragments of the cows. Pathogens most commonly isolated were Staphylococcus spp.; most of them were minor mammary pathogens. Major mammary pathogens were Streptococcus spp. (9.6%) and E. coli (2.4%). Most of the isolates were susceptible to many of 13 antimicrobial agents used, but Streptococcus spp. and Gram-negative rods showed resistance to 7 and 8 agents, respectively. 97.5% of the isolates showed susceptibility to amoxicillin, but only 55.8% to erythromycin.

• Journal of Animal Science and Technology
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• 제65권4호
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• pp.748-758
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• 2023

This study was conducted to investigate the change in activity and mounting behavior in Hanwoo (Korean Native Cattle) during the peri-estrus period and its application to estrus detection. A total of 20 Hanwoo cows were fitted with a neck-collar accelerometer device, which measured the location and acceleration of cow movements and recorded the number of instances of mounting behavior by the altitude data. The data were analyzed in three periods (24-, 6-, and 2-h periods). Blood samples were collected for 5 days after the prostaglandin F2α (PGF2α) injection, and the concentrations of estradiol, progesterone, follicle-stimulating hormone, and luteinizing hormone were determined by enzyme-linked immunosorbent assays. Activity and mounting behavior recorded over 2-h periods significantly increased as estrus approached and were more efficient at detecting estrus than over 24- and 6-h periods (p < 0.05). Endocrine patterns did not differ with the variation of individual cows during the peri-estrus period (p > 0.05). Activity was selected as the best predictor through stepwise discriminant analysis. However, activity alone is not enough to detect estrus. We suggest that a combination of activity and mounting behavior may improve estrus detection efficiency in Hanwoo. Further research is necessary to validate the findings on a larger sample size.

• Reproductive and Developmental Biology
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• 제29권1호
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• pp.37-41
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• 2005

본 연구는 한우 수정란의 체외생산에 있어서 효율과 품질의 향상을 위해서, 미성숙 난포란을 회수하는 난소의 형태와 배양 용기로서 straw의 효과를 검토하였다. 난소의 형태에 따른 수정율은 전 군에서 70.3∼84.1%로서 비슷한 경향이었다. 8세포기 및 배반포기 발달율은 황체와 난포가 모두 존재하지 않는 대조군이 가장 높았다. 배반포의 inner cell mass(ICM), trophectoderm(TE), total cell number(TCN) 및 ICM/TCN 비율은 낭종군과 퇴행황체군이 다른군에 비하여 높은 경향이었다. 체외성숙에 이용하는 배양용기에 따른 수정율은 0.5 ㎖ straw 군이, 8세포기 발달율은 대조군이 가장 높았으나, 배반포기 발달율은 23.1∼30.7％로서 각 군 간에 비슷한 경향이었다. 한편 각각의 배양 용기에서 유래된 배반포의 ICM, TE, TCN 및 ICM/TCN 비율은 유사한 경향이었다.

• 한국수정란이식학회지
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• 제20권1호
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• pp.17-24
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• 2005

본 연구는 임상 조건하에서 수정란이식의 효율성 증대를 위하여 수란우 측면의 산차, 체점수(BCS), 발정 발현과 유도, 황체 형태, 자궁 크기 및 자궁각 위치가 체외수정란이 이식된 수란우의 임신과 유산에 미치는 효과를 검토하였다. 미경산우의 임신율$(43.5\%)$이 경산우$(33.6\%)$에 비하여 유의하게 높았으나, 유산율$(12.6\;vs.\;7.5\%)$은 유사한 경향이었다. BCS에 따른 임신율은 차이가 없었으나 유산율은 BCS 3.0 미만군$(55.6\%)$이 $3.0\~3.9$군 $(10.8\%)$에 비하여 유의하게 높았다. 수란우의 발정 발현과 유도 방법, 황체 형태, 자궁 크기 및 자궁각 위치에 따른 임신율과 유산율은 유사한 경향이었다.