Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Identification of Korean native cattle persistently infected with BVDV using Ear-notch method

  • Kim, Youngsik (Laboratory of Veterinary Infectious Disease, College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Yongkwan (Environmental Health Research Department, National Institution of Environmental Research) ;
  • Lee, Sook-Young (Environmental Health Research Department, National Institution of Environmental Research) ;
  • Lee, Kyoung-Ki (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Lee, Kyung-Hyun (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency) ;
  • Song, Jae-Chan (College of Veterinary Medicine, Kyungpook National University) ;
  • Oem, Jae-Ku (Laboratory of Veterinary Infectious Disease, College of Veterinary Medicine, Chonbuk National University)
  • Received : 2018.10.05
  • Accepted : 2019.03.24
  • Published : 2019.06.30
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Abstract

Bovine viral diarrhea Virus (BVDV) infections cause respiratory, gastrointestinal, and reproductive problems, such as infertility, abortion, stillbirth, and sickly offspring. Many countries have reduced the economic damage through the application of different control programmes, and some have successfully eradicated BVD. Detection and elimination of cattle persistently infected (PI) with BVDV is important for BVD eradication because PI cattle are a main source of BVD transmission. In this study, the prevalence of Korean native cattle persistently infected (PI) with BVDV was investigated and determined in 49 farms with 3,050 cattle. The all samples were collected by ear notch sampling. Korean native cattle with initial positives on antigen-ELISA (Ag-ELISA) were sampled again after 3~4 weeks and cattle with second positives in both Ag-ELISA and immunohistochemistry (IHC) were identified as PI cattle. Among the 49 farms, 14 (28.6%) farms had at least more than one PI cow and 21 (0.69%) of 3,050 cattle were determined as PI cattle. As a result of this work, it is suggested that national BVD eradication program is required to reduce economic losses by BVDV infection in Korean cattle industries.

Keywords

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Fig. 1. Skin from the ear of Korean native cattle persistently infected with bovine viral diarrhoea virus (BVDV) Immunohistochemistry. BVDV Viral antigen was detected in the epidermal keratinocytes (black arrow head) hair follicles (black arrow head) (A), and infiltrating histiocytes (white arrow) in the dermis (B). ABC method, counterstain with Mayer's hematoxylin, ×400. Bar=100.

Table 1. The prevalence of persistently infected (PI) Korean native cattle

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