• 한국보건간호학회지
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• 제15권1호
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• pp.157-171
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• 2001

The study was designed to provide the fundamental information for understanding discomfort of bone marrow donors and for promoting an individual comfort by comparing the difference on discomfort between unrelated donors and related donors. The subject of the study was fifty related donors and thirty unrelated donors who was in the C University Hospital. This survey had been carried out and collected from October, 1998 to March, 1999. The scale of discomfort of donors associated with bone marrow donation were assessed by the questionnaire deviced by Kim Sang Dol and amended by the researcher. Data was analyzed by $x^2-test$, t-test, two-way ANOVA, and Pearson Correlation Coefficients. The results were as follows. 1. Considering the general characteristics of bone marrow donors according to gender, male was consisted of $60\%$ and female was consisted of $40\%$. Of those related donors are consisted for $62.5\%$ and accounted for $37.5\%$ of those unrelated donors. Considering the classification to the job, employee of company were major donors which was $35\%$, and next order was student, individual businessman, and housewife. Considering the education level. college students were $48.7\%$ and students who have less than high school level were $42.5\%$. 2. According to the above the results regarding discomfort of bone marrow donation, it is especially shown that the major cause for discomfort of bone marrow donors is on physical factor. The concrete examples for physical factor are pain in the region of bone marrow harvest and pain in the injection part by fluid therapy and blood-sampling, an immovability of the body after bone marrow harvest, and difficulties on walking. Considering physiological factor, there are an uneasiness about leading to injure their health, vague fear about the hospital. and a tedium at hospital. Environmental factors for discomfort of bone marrow donors are insufficient explanation for needle gauge and procedure of bone marrow donation and difficulty on following medical schedule. Therefore. it is necessary to establish more effective and systematically organized program for nursing intervention based on the research results. An effective program is only useful in getting rid of discomfort of bone marrow donors.

• 한국지반신소재학회논문집
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• 제17권1호
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• pp.21-31
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• 2018

본 연구에서는 시공이 완료된 CFRD에 대하여 담수시점부터 수위변화에 따른 댐 제체의 변형거동을 파악하기 위하여 현장 모니터링을 통하여 댐의 변위를 분석하고, 이를 수치해석 결과와 비교하였다. 담수에 따른 댐 정상 및 하류사면 제체의 거동을 계측한 결과, 축방향 수평변위, 상 하류측 수평변위, 침하가 대부분 초기 담수로부터 저수위가 댐 높이 절반 정도에 이를 때부터 발생하였다. 그 후 저수위 최고 상승시까지 변위가 진행된 후 수위에 관계없이 일정하게 수렴되는 양상을 보였다. CFRD에서 가장 중요한 역할을 하는 차수벽의 수평변위는 모든 지점에서 비슷한 양상을 보였다. 차수벽의 수평변위는 담수 전 외부온도의 영향으로 동절기에 증가하고 하절기에 감소하는 경향이 있었다. 또한, 담수 후 저수위가 댐 높이 절반 정도에 이를 때까지 변위가 증가한 후 수위상승과 함께 감소하는 경향을 보였다. 결과적으로 차수벽의 거동은 저수위 조건뿐 아니라 담수 후에도 계절적인 변화를 나타내며, 콘크리트 슬래브의 재료적인 특성 영향이 큰 것으로 판단된다. 수치해석 결과 댐 축조 후 최대 침하량과 발생 위치는 실제와 다소의 차이가 있었는데, 이러한 차이는 해석시 입력 매개변수의 추정, 축조일수 및 층 시공두께 등 다양한 설계와 시공의 차이에서 기인한 것으로 판단된다. 그리고 전반적인 기간에 대하여 침하는 댐 축조기간 동안 대부분 완료되었고 담수 초기에 약간의 침하가 발생한 후 수렴되었다.

• 한국식품영양과학회지
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• 제38권8호
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• pp.1003-1007
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• 2009

부추를 암 예방 식품 소재로 활용하기 위하여 부추로부터 주요 생리활성 물질인 함황화합물을 분리하여 인체 암세포성장 억제 및 간암세포의 사멸이 apoptosis에 의해 유도되는지를 조사하였다. 부추 함황화합물을 1, 5, 10, 20 및 30 $\mu g$/mL 농도로 24, 48 및 72시간별로 간암(HepG2) 및 폐암세포 (A549)에 처리하여 암세포 증식억제 효과를 측정한 결과 HepG2 및 A549 세포에서 농도 및 시간 의존적으로 그 성장을 억제하였으며, 20 $\mu g$/mL 농도 이상에서 암세포 성장이 60% 이상 억제되었다. 또한 부추 함황화합물 30 $\mu g$/mL 농도로 처리 시 대조군에 비하여 폐암 및 간암 세포수 감소 및 심한 형태학적 변화가 관찰되었다. 이들 암세포의 $IC_{50}$ 값을 측정한 결과 부추 함황화합물은 폐암세포(A549)보다 간암세포(HepG2)에 더 효과가 있었다. 한편 부추 함황화합물은 30 $\mu g$/mL 농도에서 핵 응축 및 apoptotic body를 나타내었으며, 농도 의존적으로 subG1 DNA 함량이 증가함으로써 HepG2 암세포 사멸이 apoptosis에 의해 유도되는 것을 확인할 수 있었다.

• 한국식품과학회지
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• 제43권5호
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• pp.641-647
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• 2011

본 연구에서는 녹차의 주요 생리활성 성분인 PPE와 일반 의약품 성분 AAP, Asp 및 Ibu와의 혼용 시 일어날 수 있는 상호 작용에 의한 세포독성 변화를 장관계 정상세포와 암세포 모델에서 비교하였다. PPE는 정상 장관계 세포 INT 407 및 대장암 세포 HCT 116에 농도의존적인 독성을 나타내었고, $IC_{50}$ 수치는 각각 29.9과 57.4 ${\mu}g/mL$로써 암세포에 더욱 강력한 독성을 보였다. PPE와 200 ${\mu}M$ 이하의 저농도 약물을 HCT 116 세포에 24, 48시간 복합 투여한 결과 전체적으로 현저한 독성의 변화현상은 발생하지 않았다. HCT 116 세포에서 항산화효소 SOD 존재 시에 PPE 독성의 유의적인 감소가 관찰되었으나, 이 조건에서도 약물에 의한 유의적인 변화는 나타나지 않았다. PPE로부터 생성되는 $H_2O_2$의 양은 5 mM 이상의 Asp 또는 Ibu에 의하여 감소하였고, AAP에는 영향을 받지 않았다. INT 407 세포와 HCT 116 세포에서 mM 이상의 고농도 약물과 PPE를 복합 처리하여 세포독성변화를 본 결과 현저한 독성의 변화가 관찰되지 않았으나, INT 407 세포에서 PPE 존재 시 AAP의 독성이 다소 증가하였다. 한편 PPE와 약물을 각각 순서를 달리하여 전후로 처리하였을 때 HCT 116 세포에서의 독성이 단독처리 시 각각의 합보다 20% 이내에서 증가하였다. PPE와 빈번히 복용되는 일반 의약품 AAP, Asp 및 Ibu를 여러 조합에 의해 장관계 세포에 처리하였을 때 이들의 상호작용에 의해 일부 독성의 강화현상이 나타났으나 전체적으로 두드러진 독성발현 빛 활성변화는 발견되지 않았다.

• 한국안전학회지
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• 제31권4호
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• pp.111-119
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• 2016

This study aimed to analyze postures that were frequently conducted in manufacturing industry. To find grip strength and muscle activities of each posture, Maximum Voluntary Contraction (MVC) and ElectroMyoGraphy (EMG) were measured. Based on the results of this study, the most appropriate posture could be suggested and used as a basic information for preventing musculoskeletal disorders. Most work-related musculoskeletal disorders have been occurred in the fields of manufacturing industry. According to previous studies, it was reported that the rate of musculoskeletal diseases of upper extremity was higher than that of other body parts. Accordingly, there were many studies about discomfort and grip strength of upper extremity. However, these studies dealt with single selection of wrist, elbow and shoulder. So, it was insufficient for comprehensive studies about upper extremity. And in order to improve the work posture, the physiological changes being generated by the combination of wrist, elbow and shoulder postures should be observed and analyzed. In order to conduct this study, thirty university students who had no records of MSDs involved were recruited. Independent variables were postures of wrist(pronation, neutral, supination), postures of elbow(flexion $45^{\circ}$, $90^{\circ}$) and postures of shoulder(flexion $0^{\circ}$, $90^{\circ}$). And dependent variables were MVC values and EMG values. Jamar dynamometer and TeleMyo 2400T G2 was used to measure MVC and EMG. MVC and EMG for 12 postures were measured for three second and for three times. Experiment was performed randomly. A 10 minutes rest period was provided after each t. To measure muscle load, the EMG signals of eight muscles (Biceps, Medial triceps, Lateral triceps, Brachioradialis, Extensor carpi ulnaris, Extensor carpi radialis, Flexor carpi ulnaris and Flexor carpi radialis) were evaluated. MVC values and EMG values were analyzed using Minitab ver. 14. The results showed that MVC value was the highest at shoulder $0^{\circ}$, elbow $45^{\circ}$ and wrist supination. In case of wrist postures, MVC of supination is the highest. In case of elbow and shoulder postures, MVC of flexion $45^{\circ}$ and $0^{\circ}$ was the highest. It was found that there were interaction between wrist and elbow posture under shoulder flexion and between shoulder and wrist under elbow flexion $45^{\circ}$. In case of the angle of shoulder $0^{\circ}$, elbow $45^{\circ}$ and wrist supination, the EMG values of four muscles(Medial Triceps, Extensor carpi ulnaris, Extensor carpi radialis, Flexor carpi ulnaris) were the highest. Based on this study, it is worth to note that the combination postures of upper extremity have a large impact on the MVC and EMG. The optimal condition upper extremity was shoulder flexion $0^{\circ}$, elbow flexion $45^{\circ}$ and wrist supination for preventing work-related musculoskeletal disease.

• 대한환경공학회지
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• 제34권12호
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• pp.855-862
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• 2012

Free-living 선충으로 알려진 Caenorhabditis elegans는 주로 토양 공극수에서 서식하며, 토양 영양단계, 에너지 흐름, 그리고 분해자로서 중요한 역할을 하는 것으로 알려져 있다. 최근 들어, C. elegans는 나노독성연구에 널리 사용되고 있는 추세이다. 본 연구에서는 C. elegans를 이용한 나노독성과 그에 대한 기작에 관련된 선행연구를 조사하였으며, 총 20건의 연구를 확인하였다. 대부분의 연구는 K-medium, S-medium, 그리고 NGM (Nematode Growth Medium) plate를 노출매체로 이용하고 있으며, 나노물질에 노출된 C. elegans로부터 관찰된 영향으로는 노화억제, 광독성영향, 유전독성, 그리고 표피자극 등이 포함되었다. C. elegans를 이용한 독성기작 연구는 개체 생활사 영향 평가, 산화스트레스 영향 평가, 유전독성영향 평가, 나노물질의 생체 내 분포, 그리고 나노물질 특성에 의한 독성영향 평가로 분류할 수 있다. 본 연구에서는 다양한 세포활성, 잘 알려진 유전정보, 그리고 투명한 구조로 인한 나노물질 관찰의 용이성을 바탕으로, C. elegans를 이용한 나노독성연구의 장점을 확인하였다. C. elegans는 나노독성을 평가하기에 적합한 시험종으로 고려되고 있다.

• 대한치과마취과학회지
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• 제12권2호
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• pp.93-97
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• 2012

Background: Some disabled patients show insufficient cooperation during dental treatment, and general anesthesia in an outpatient setting can be successfully administered. To minimize post-anesthetic complications is an essential issue, and strict discharge protocols are required for the safety of the patients. Post-anesthetic follow-ups using telephone calls can be applied to improve the quality of the outpatient care system. The authors evaluated the post-operative condition of patients after dental treatment under general anesthesia. Methods: Total 143 patients and their caregivers included in this study. The patients received general anesthesia for dental treatment in Seoul National University Dental Hospital, Clinic for Persons with Disabilities from July, 2011 to April, 2012. Telephone calls were given to the patients or their caregivers to collect information about the patients' systemic condition and anesthesia-related complications. Results: Among 131 patients with responses of telephone calls, 87 patients (66.4%) reported no discomfort, while 44 patients (33.6%) presented post-anesthetic complications. A total of 20 patients reported mild fever, 10 patients had vomiting, and 7 patients had sore throat. Other complications included nausea, fatigue, nasal bleeding, skin sore, and body rash. Among the patients with the history of epilepsy, 63.6% showed post-anesthetic discomfort or complication (P = 0.027, ${\chi}^2$ test). Conclusions: One third of dental patients who received general anesthesia due to insufficientcooperation complained discomfort after discharged from outpatient anesthetic care.

• 한국보건간호학회지
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• 제17권2호
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• pp.238-254
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• 2003

The objective of this study was to provide baseline information useful for developing education programs to prevent sexual harassment in schools. Survey of recognition and experiences of sexual harassment and analysis of factors associated with sexual harassment were conducted. The data were collected using questionnaires from December 9 through 28, 2002. A total of 737 female teachers in Daegu participated in the survey. The data were analyzed with descriptive analysis, ANOVA, and T-test using SPSS /WIN 11.0. The study results were as follows: 1. As to the characteristics related to sexual harassment, $51.3\%$ of the female teachers had no experience of preventive education about sexual harassment, $37.2\%$ of the female teachers perceived that enactment of related laws had little influence on the prevalence of sexual harassment, and $59.8\%$ thought strict punishments are required. For teachers who have experienced sexual harassment in schools, $39.8\%$ thought that they must report to the counseling office or female teachers' meeting. On the other hand, $35.7\%$ answered that they would take passive actions such as talking to their friends or colleagues. 2. As to the mode of sexual harassment, participants regarded physical harassment $(4.06\pm0.78)$ as the prominent forms of sexual harassment, followed by visual harassment $(4.04\pm0.77)$, and verbal harassment $(3.86\pm0.81)$. Among physical harassment. 'touching a body part (shoulder, hip, hand, etc)(4.58) was recognized as prominent forms of physical harassment, while 'blocking one's path or impeding one's movement (3.48)' was recognized as least prominent forms of physical harassment. 3. As to the experiences of sexual harassment, $79.8\%$ of the female teachers had experienced sexual harassment. According to the characteristics of schools. public junior high school $(87.8\%)$, private high school $(83.4\%)$ and bisexual school $(82.5\%)$ had higher rates of sexual harassment. 4. As to the characteristics of experiences in sexual harassment. $65.3\%$ thought that sexual harassment was not serious, but $18.9\%$ thought it was serious and $2.2\%$ thought as very serious. 5. As to the characteristics of sexual harassers, $378.6\%$ were 'colleague teachers' and $8.6\%$ were 'principals and/or vice-principals'. Ninety-three percent of sexual harassers were , male' and $40.2\%$ of them were 'in their 40s'. In most cases. the offender of the sexual harassment was male teachers in the same schools. And more than half of the female teachers replied they had never had any education related to sexual harassment. which means that systematic education is quite urgent.

• 한국과학교육학회지
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• 제35권6호
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• pp.997-1006
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• 2015

실험에 대한 메타적 인식은 학생들의 과학적 탐구와 과학의 본성에 대한 이해의 중요한 요소 중 하나이다. 이 연구에서는 중학생과 고등학생의 과학과 생물에서의 '실험'의 의미에 대한 인식을 알아보았다. 특히 이 연구에서는 언어 네트워크 분석방법을 사용하여 학생들의 인식을 구조적으로 확인하였다. 이 연구를 위해 190명의 중학생과 200명의 고등학생 이 연구에 참여하였다. 학생들은 과학에서의 '실험'과 생물에서의 '실험'의 의미에 대한 두 문항에 서술형으로 응답하였다. 수집된 응답을 바탕으로 총 4개의 언어 네트워크가 구성되었다. 연구 결과, 과학에서의 '실험'에 대하여 중학생들은 '우리', '직접', '원리' 등의 단어를 중심으로 활동적 측면에서의 실험을 인식하였다. 반면 고등학생은 '이론', '사실', '내용' 등의 단어를 중심으로 지식을 생성하는 탐구과정으로서의 실험으로 인식하였다. 또한 생물에서의 '실험'에 대하여 중학생은 '해부', '몸'을 중심으로, 고등학생은 '생명', '관찰'이 중심으로 인식하여 생명체를 다루는 관찰활동으로 인식하였다. 이러한 연구결과는 앞으로 과학 교과 및 생물 교과에서 실험을 지도하는데 있어서 중요한 근거자료로 활용될 수 있을 것이다.

• 생물정신의학
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• 제25권3호
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• pp.60-71
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• 2018

Objectives A growing body of evidence has suggested that morphologic changes in cerebellum may be implicated with pathophysiology of major depressive disorder (MDD). The aim of this study is to investigate a difference in the volume and cortical thickness of the specific region of cerebellum between patients with MDD and healthy controls (HC). Methods A total of 127 patients with MDD and 105 HC participated in this study and underwent T1-weighted structural magnetic resonance imaging. We analyzed volume and cortical thickness of each twelve cerebellum regions divided by left and right and the volume and cortical thickness of the whole cerebellum from T1-weigted image of participants. One-way analysis of covariance was used to investigate the volume and cortical thickness difference of total and specific regions between two groups adjusting for age, gender, medication, and total intracranial cavity volume. Results We found that the patients with MDD had significantly greater volume in the left cerebellum lobule III region [false discovery rate (FDR)-corrected p = 0.034] compared to HC. Also, our findings indicate that cortical thickness of left lobule VIIB (FDR-corrected p = 0.032) and lobule VIIIB (FDR-corrected p = 0.032) are significantly thinner in the patients with MDD compared with the HC. No significant volume and cortical thickness differences were observed in other sub-regions of the cerebellum. The volumes and cortical thickness of whole cerebellum between patients with MDD and HC did not differ significantly. Conclusions We observed the region-specific volume and cortical thickness difference in cerebellum between the patients with MDD and HC. The results of our study implicate that the information about structural alterations in cerebellum with further replicative studies might provide a stepping stone toward a specific marker to diagnose MDD.