• Journal of Life Science
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• v.19 no.8
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• pp.1039-1046
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• 2009

The cytochrome P450s (P450s) metabolizing natural products are among the most versatile biological catalysts known in plants, but knowledge of the structural basis for their broad substrate specificity has been limited. The activity of p-coumarate 3-hydroxylase (C3H) is thought to be essential for the biosynthesis of lignin and many other phenylpropanoid pathway products in plants however, all attempts to express and purify the protein corresponding C3H gene have failed. As a result, no conditions suitable for the unambiguous assay of the enzyme are known. The detailed understanding of the mechanism and substrate-specificity of C3Hdemands a method for the production of active protein on the milligram scale. We have developed a bacterial expression and purification system for the plant C3H, which allows for the quick expression and purification of active wild-type C3H via introduction of combinational mutagenesis. The modified cytochrome P450 C3H ($C3H_{mod}$) could be purified in the absence of detergent using immobilized metal affinity chromatography and size exclusion chromatography following extraction from isolated membranes in a high salt buffer and catalytically activated. This method makes the use of isotopic labeling of C3H for NMRstudies and X-ray crystallography practical, and is also applicable to other plant cytochrome P450 proteins.

• Journal of Oral Medicine and Pain
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• v.36 no.3
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• pp.169-176
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• 2011

Oromandibular dystonia (OMD) is a form of focal dystonia that affects the masticatory, facial and lingual muscles in any variety of combinations, which results in repetitive involuntary and possibly painful jaw opening, closing, deviation or a combination of these movements. This study aimed to investigate clinical features and treatment type of OMD patients. By retrospective chart review, the study was conducted to consecutive OMD patients who visited a department of Oral Medicine and Orofacial Pain Clinic in a university dental hospital during Aug 2007 to Apr 2010. 78 OMD patients were identified with female preponderance (M:F=1:3.6) and a mean age of 72 years. Their mean duration of OMD was about 10 months. The most common chief complaints at the first visit was jaw ache, followed by uncontrolled, repetitive movement of the jaw and/or oral tissues, pain in the oral region(p=0.000). The most common subtype of OMD was lateral jaw-deviation dystonia, followed by combination and jaw-closing dystonia(p=0.001). While no apparent cause was recognized in over 60% of the OMD patients, peripheral trauma including dental treatment such as prosthetic treatment and extraction was the most frequently reported as precipitating factor(p=0.000). Medication was the 1st line therapy for our patients and anxiolytics such as clonazepam was given to most of them. Based on the results of this study, OMD is the disease of the elderly, particularly of women and causes orofacial pain and compromises function of orofacial region. Some patients considered dental treatment a precipitating factor. Dentists, therefore, should have knowledge of symptoms and treatment of OMD.

• Journal of the Korean Institute of Landscape Architecture
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• v.46 no.6
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• pp.41-48
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• 2018

The aim of this study is to provide foundational knowledge of how to enhance the user satisfaction of urban parks. The study seeks to identify essential factors that influence user satisfaction and to provide better design strategies for future park design as well as the reorganization of existing ones. To measure user satisfaction, this study used factor analysis to extract essential factors - facility conditions, landscape and scenery, safety, and kindness - by using data from a survey conducted by the city of Seoul in 2017. We then used a regression analysis to infer causal relationships between the independent variables and the dependent variables (user satisfaction). The results revealed that the most significantly and positively related variable to user satisfaction in urban parks was safety (${\beta}=0.276$, p<.000), followed by landscape and scenery (${\beta}=0.230$, p<.000), facility conditions (${\beta}=0.215$, p<.000), and kindness (${\beta}=0.208$, p<.000). The results indicate that, for future urban park designs, planners and designers should prioritize the issues of safety by adopting crime prevention through environmental design (CPTED). In addition, planners and designers of future park designs should heavily weigh the selection and provision of relevant facilities for the intended use as well as well-arranged and well-managed plants and trees. Based on the results of IPA analysis, the most urgent improvement elements appeared to be the factor of kindness; however, the impact of kindness influencing user satisfaction was less important than that of safety and landscape and scenery in the urban park design processes. This study demonstrates that to maximize the user satisfaction of the urban park design processes and to provide more valuable spaces for users, it is necessary to secure park safety and to create well-composed landscape and scenery. Future research should provide more detailed and specified urban park design strategies corresponding with the importance of the factors identified in this study.

• Journal of Korean Home Economics Education Association
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• v.34 no.3
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• pp.1-23
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• 2022

This study examined the perceptions of Myanmar university students and professors regarding the status and necessity of higher education programs in fashion. Data were collected from professors in textile engineering at Yangon Technological University and Myanmar university students. Closed- and open-ended questions were asked either through interviews or by email. The responses were analyzed using keyword extraction and categorization, and descriptive statistics(closed questions). Generally, the professors perceived higher education, as well as the cultural industries including art and fashion, as important for Myanmar's social and economic development. According to the students interests in pursuing a degree in textile were limited, despite the high interest in fashion. Low wages in the apparel industry and lack of fashion degrees that meet the demand of students were cited as reasons. The demand was high for educational programs in fashion product development, fashion design, pattern-making, fashion marketing, branding, management, costume history, and cultural studies. Students expected to find their future career in textiles and clothing factories. Many students wanted to be hired by global fashion brands for higher salaries and training for advanced knowledge and technical skills. They perceived advanced fashion education programs will have various positive effects on Myanmar's national economy.