• Title/Summary/Keyword: Knock-in

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Quasidimensional Simulation with Multi-zone Combustion Model for Homogeneous GDI Engine Emissions and Knocking (균일혼합기 가솔린 직분사 엔진의 다중 영역 유사차원 해석을 통한 배기 및 노킹 예측)

  • Lee, Jaeseo;Huh, Kang Y.;Kwon, Hyuckmo;Park, Jae In
    • Journal of the Korean Society of Combustion
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    • v.18 no.1
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    • pp.7-12
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    • 2013
  • A quasidimensional program is developed for a four stroke cycle homogeneous GDI (Gasoline Direct Injection) engine. It includes models for spray, burning rate and chemistry to predict knock and emissions. With early injection a homogeneous GDI engine goes through spark ignited, turbulent premixed combustion as in PFI (Port Fuel Injection) engines. The cylinder charge is divided into unburned and burned zone with the latter divided into multiple zones of equal mass to resolve temperature stratification. Validation is performed against measured pressure traces, NOx and CO emissions at different load and RPM conditions. Comparison is made between an empirical knock model and predictions by the chemistry model in this work.

A Study on the Characteristic of Beakdown Voltage for Combustion Diagnostic of Gasoline Engine (가솔린기관의 연소현상 진단을 위한 브레이크다운 전압의 특성에 관한 연구)

  • Park, Jae-Keun;Jo, Min-Seok;Whang, Jae-Won;Jang, Gi-Hyun;Chae, Jae-Ou
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.24 no.9
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    • pp.1157-1165
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    • 2000
  • A classic examples of the abnormal combustions are the knock and misfire, which raise noxious performance and life of the engine. A heavy knock can also cause severe damages to the engine itself, which gives more reason why it must be detected and corrected. With the response of the today's requirements, we have researched the new diagnostic system which uses the breakdown voltage characteristics between electrodes of spark plug. This breakdown voltage depends on the pressure, temperature and even the shape and material of electrodes. But there is no data of breakdown voltage in case of using the spark plug as a electrodes. So, in this study, we show the breakdown voltage characteristic by pressure and temperature in constant volume bomb, which will make it possible to diagnose the engine combustion phenomenon.

Diesel Knock Visualization of Premixed Charge Compression Ignition Combustion with a Narrow Injection Angle (협각 인젝터를 이용한 예혼합 압축착화 연소에서의 디젤 노킹 가시화)

  • Park, Stephen S.;Jung, Yongjin;Bae, Choongsik
    • 한국연소학회:학술대회논문집
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    • 2012.04a
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    • pp.101-104
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    • 2012
  • In this work, in-cylinder pressure measurements and high-speed direct imaging of the flame were performed in an optically accessible single cylinder diesel engine with premixed charge compression ignition combustion and a narrow injection angle. The results show that the frequency ranges of pressure ringing were 8.35 to 9 kHz and 12..2 to 13.1 kHz. The frequencies of the flame movement were shown as 8.7 kHz and 13 kHz. It was found that there is a direct relationship between the pressure ringing and the flame movement.

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Use of Tumor Necrosis Factor Receptor (TNFR)-Knockout Mice to Probe the Mechanism of Chemically-Induced Asthma

  • Karol, Meryl H.;Matheson, Joanna M.;Lange, Robert W.;Lemus, Ranulfo;Luster, Michael I.
    • Toxicological Research
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    • v.17
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    • pp.305-307
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    • 2001
  • Toluene diisocyanate (TDI) is widely used in the manufacture of polyurethanes and is a recognized cause of occupational asthma. Although extensive investigations have been undertaken, the molecular mechanism(s) of the disease is still unclear. We hypothesized that inflammatory cytokines are required during both the sensitization and elicitation phases of the disease and have utilized TNF-R knock-out (KO) mice to address the hypothesis. Black C57 TNFR knock-out mice were exposed to TDI by sc injection and challenged by inhalation of 100 ppb TDI vapor. Control animals included: wild type C57 animals, sham-exposed animals that were challenged with TDI, and animals that were injected with anti-TNF antibodies prior to sensitization and again prior to challenge. Total IgE was increased in the knock-out animals compared with the wild type sensitized and challenged animals whereas TDI-specific IgG antibodies did not differ significantly in KO and wild type animals. There was less inflammation in the nares and trachea in KO animals compared with the wild type animals exposed to TD1 as well as less goblet cell hyperplasia and epithelial damage. Airway reactivity was assessed in animals treated with anti-TNF$\alpha$ antibody and found to be substantially reduced compared with that in sensitized and challenged animals. These results indicate that TNF$\alpha$ plays a role in the immunologic and physiologic responses and in airways inflammation in this animal model and suggests a role for TNF in occupational asthma due to TDI.

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Regulation of HIF-1α stability by lysine methylation

  • Baek, Sung Hee;Kim, Keun Il
    • BMB Reports
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    • v.49 no.5
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    • pp.245-246
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    • 2016
  • The level and activity of critical regulatory proteins in cells are tightly controlled by several tiers of post-translational modifications. HIF-1α is maintained at low levels under normoxia conditions by the collaboration between PHD proteins and the VHL-containing E3 ubiquitin ligase complex. We recently identified a new physiologically relevant mechanism that regulates HIF-1α stability in the nucleus in response to cellular oxygen levels. This mechanism is based on the collaboration between the SET7/9 methyltransferase and the LSD1 demethylase. SET7/9 adds a methyl group to HIF-1α, which triggers degradation of the protein by the ubiquitin-proteasome system, whereas LSD1 removes the methyl group, leading to stabilization of HIF-1α under hypoxia conditions. In cells from knock-in mice with a mutation preventing HIF-1α methylation (Hif1αKA/KA), HIF-1α levels were increased in both normoxic and hypoxic conditions. Hif1αKA/KA knock-in mice displayed increased hematological parameters, such as red blood cell count and hemoglobin concentration. They also displayed pathological phenotypes; retinal and tumor-associated angiogenesis as well as tumor growth were increased in Hif1αKA/KA knock-in mice. Certain human cancer cells exhibit mutations that cause defects in HIF-1α methylation. In summary, this newly identified methylation-based regulation of HIF-1α stability constitutes another layer of regulation that is independent of previously identified mechanisms.

TRAIL Suppresses Human Breast Cancer Cell Migration via MADD/CXCR7

  • Wang, Rui;Li, Jin-Cheng
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.7
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    • pp.2751-2756
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    • 2015
  • Background: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) can specifically induce apoptosis limited to various cancer cells, so this reagent is considered a promising medicine for cancer therapy. TRAIL also exerts effects on non-apoptotic signals, relevant to processes such as metastasis, autophagy and proliferation in cancer cells. However, the mechanisms of TRAIL-regulated non-apoptotic signals are unclear. The purpose of this study was to investigate MADD/CXCR7 effects in TRAIL-mediated breast cancer cell migration. Materials and Methods: The ability of MADD/CXCR7 to regulate MVP signaling in TRAIL-mediated breast cancer cells migration was evaluated by transwell migration assay, quantitative RT-PCR, Western blotting and knock down experiments. Results: In this study, we found that treatment with TRAIL resulted in induced expression levels of MADD and CXCR7 in breast cancer cells. Knock down of MADD followed by treatment with TRAIL resulted in increased cell migration compared to either treatment alone. Similarly, through overexpression and knockdown experiments, we demonstrated that CXCR7 also positively regulated TRAIL-inhibited migration. Surprisingly, knock down of MADD lead to inhibition of TRAIL-induced CXCR7 mRNA and protein expression and overexpression of CXCR7 lead to the reduction of MADD expression, indicating that MADD is an upstream regulatory factor of TRAIL-triggered CXCR7 production and a negative feedback mechanism between MADD and CXCR7. Furthermore, we showed that CXCR7 is involved in MADD-inhibited migration in breast cancer cells. Conclusions: Our work defined a novel signaling pathway implicated in the control of breast cancer migration.

Susceptibility of pentylenetetrazole-induced seizures in mice with Cereblon gene knockout

  • Jeon, Seung-Je;Ham, Jinsil;Park, Chul-Seung;Lee, Boreom
    • BMB Reports
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    • v.53 no.9
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    • pp.484-489
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    • 2020
  • Epilepsy is a neurological disorder characterized by unpredictable seizures, which are bursts of electrical activity that temporarily affect the brain. Cereblon (CRBN), a DCAFs (DDB1 and CUL4-associated factors), is a well-established protein associated with human mental retardation. Being a substrate receptor of the cullin-RING E3 ubiquitin ligase (CRL) 4 complex, CRBN mediates ubiquitination of several substrates and conducts multiple biological processes. In the central nervous system, the large-conductance Ca2+-activated K+ (BKCa) channel, which is the substrate of CRBN, is an important regulator of epilepsy. Despite the functional role and importance of CRBN in the brain, direct injection of pentylenetetrazole (PTZ) to induce seizures in CRBN knock-out mice has not been challenged. In this study, we investigated the effect of PTZ in CRBN knock-out mice. Here, we demonstrate that, compared with WT mice, CRBN knock-out mice do not show the intensification of seizures by PTZ induction. Moreover, electroencephalography recordings were also performed in the brains of both WT and CRBN knockout mice to identify the absence of significant differences in the pattern of seizure activities. Consistently, immunoblot analysis for validating the protein level of the CRL4 complex containing CRBN (CRL4Crbn) in the mouse brain was carried out. Taken together, we found that the deficiency of CRBN does not affect PTZ-induced seizure.

Development of a toxA Gene Knock-out Mutant of Pasteurella multocida and Evaluation of its Protective Effects

  • Kim Tae-Jung;Lee Jae-Il;Lee Bong-Joo
    • Journal of Microbiology
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    • v.44 no.3
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    • pp.320-326
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    • 2006
  • Pasteurella multocida is an important veterinary and opportunistic human pathogen. In particular, strains of P. multocida serogroup D cause progressive atrophic rhinitis, and produce a potent, intracellular, mitogenic toxin known as P. multocida toxin (PMT), which is encoded by the toxA gene. To further investigate the toxigenic and pathogenic effects of PMT, a toxA-deleted mutant was developed by homologous gene recombination. When administrated to mice, the toxigenicity of the toxA mutant P. multocida was drastically reduced, suggesting that the PMT constributes the major part of the toxigenicity of P, multocida. Similar results were obtained in a subsequent experiment, while high mortalities were observed when toxA(+) P. multocida bacterial culture or culture Iysate were administrated. Mice immunized with toxA(-) P. multocida were not protected (none survived) following challenge with toxA(+) P. multocida or bacterial culture Iysate (toxin). These results suggest that the toxigenicity of P. multocida is mainly derived from PMT.

Inhibition of Oligomycin Biosynthesis by olmA5 Gene Knock-out in Streptomyces avermitilis (Streptomyces avermitilis에서 olmA5 Gene의 Knock-out에 의한 Oligomycin 합성 억제)

  • Kang, Hyun-Woo;Ryu, Yeon-Woo
    • KSBB Journal
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    • v.24 no.3
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    • pp.279-286
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    • 2009
  • Streptomyces is well known for their ability to synthesize enormous varieties of antibiotics as secondary metabolites. Among them, S. avermitilis produces avermectins, a group of antiparasitic agents used in human and veterinary medicine. However, S. avermitilis also produces oligomycin, which is a potential toxic inhibitor of oxidative phosphorylation in mammalian cells. Therefore, we decided to disrupt oligomycin synthetase gene to prevent co-production of oligomycin in S. avermitilis. To create plasmid for disruption, the smallest gene of oligomycin synthetase gene cluster was obtained by PCR from S. avermitilis chromosome. Then, apramycin resistance gene was inserted in oligomycin synthetase gene for selection. After transformation of this plasmid, oligomycin synthetase gene (olmA5) in the chromosome was displaced with disruption cassette on the plasmid via homologous recombination. As a result of this gene replacement, we obtained mutants (olmA5::apra) that no longer makes the toxic oligomycin. And the mutants confirmed by PCR and HPLC analysis. However, showed no increasement of avermectin production in the mutant was observed.

M6A reader hnRNPA2/B1 is essential for porcine embryo development via gene expression regulation

  • Kwon, Jeongwoo;Jo, Yu-Jin;Yoon, Seung-Bin;You, Hyeong-ju;Youn, Changsic;Kim, Yejin;Lee, Jiin;Kim, Nam-Hyung;Kim, Ji-Su
    • Journal of Animal Reproduction and Biotechnology
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    • v.37 no.2
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    • pp.121-129
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    • 2022
  • Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2/B1) is an N6-methyladenosine (m6A) RNA modification regulator and a key determinant of prem-RNA processing, mRNA metabolism and transportation in cells. Currently, m6A reader proteins such as hnRNPA2/B1 and YTHDF2 has functional roles in mice embryo. However, the role of hnRNPA2/B1 in porcine embryogenic development are unclear. Here, we investigated the developmental competence and mRNA expression levels in porcine parthenogenetic embryos after hnRNPA2/B1 knock-down. HhnRNPA2/B1 was localized in the nucleus during subsequent embryonic development since zygote stage. After hnRNPA2/B1 knock-down using double stranded RNA injection, blastocyst formation rate decreased than that in the control group. Moreover, hnRNPA2/B1 knock-down embryos show developmental delay after compaction. In blastocyste stage, total cell number was decreased. Interestingly, gene expression patterns revealed that transcription of Pou5f1, Sox2, TRFP2C, Cdx2 and PARD6B decreased without changing the junction protein, ZO1, OCLN, and CDH1. Thus, hnRNPA2/B1 is necessary for porcine early embryo development by regulating gene expression through epigenetic RNA modification.