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http://dx.doi.org/10.12750/JARB.37.2.121

M6A reader hnRNPA2/B1 is essential for porcine embryo development via gene expression regulation  

Kwon, Jeongwoo (Primate Resources Center (PRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Jo, Yu-Jin (Primate Resources Center (PRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Yoon, Seung-Bin (Primate Resources Center (PRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB))
You, Hyeong-ju (Primate Resources Center (PRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Youn, Changsic (Primate Resources Center (PRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Kim, Yejin (Primate Resources Center (PRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Lee, Jiin (Primate Resources Center (PRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Kim, Nam-Hyung (Guangdong Provincial Key Laboratory of Large Animal models for Biomedicine, Wuyi university)
Kim, Ji-Su (Primate Resources Center (PRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Publication Information
Journal of Animal Reproduction and Biotechnology / v.37, no.2, 2022 , pp. 121-129 More about this Journal
Abstract
Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2/B1) is an N6-methyladenosine (m6A) RNA modification regulator and a key determinant of prem-RNA processing, mRNA metabolism and transportation in cells. Currently, m6A reader proteins such as hnRNPA2/B1 and YTHDF2 has functional roles in mice embryo. However, the role of hnRNPA2/B1 in porcine embryogenic development are unclear. Here, we investigated the developmental competence and mRNA expression levels in porcine parthenogenetic embryos after hnRNPA2/B1 knock-down. HhnRNPA2/B1 was localized in the nucleus during subsequent embryonic development since zygote stage. After hnRNPA2/B1 knock-down using double stranded RNA injection, blastocyst formation rate decreased than that in the control group. Moreover, hnRNPA2/B1 knock-down embryos show developmental delay after compaction. In blastocyste stage, total cell number was decreased. Interestingly, gene expression patterns revealed that transcription of Pou5f1, Sox2, TRFP2C, Cdx2 and PARD6B decreased without changing the junction protein, ZO1, OCLN, and CDH1. Thus, hnRNPA2/B1 is necessary for porcine early embryo development by regulating gene expression through epigenetic RNA modification.
Keywords
blastocyst; early embryo development; epigenetic; gene expression; RNA methylation;
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