• Transactions of the Korean Society of Automotive Engineers
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• v.5 no.1
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• pp.186-194
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• 1997

The objective of this study is to develope knock control algorithms which can increase engine power without causing frequent knock occurrence. A four cylinder spark-ignition engine is used for the experiments to develop knock control algorithms which use block vibration signals. Knock occurrence is detected accurately by using knock threshold values which consider the difference of transmission path of each cylinder. Spark timing is controlled both simultaneously and individually. With the simultaneous control, torque gain is achieved by retarding the spark timing on knock occurrence in propotion to the knock intensity. The individual knock control algorithm results in higher torque gain than the simultaneous knock control algorithm. The knock occurrence frequency of the individual knock control algorithm is about twice the value of the simultaneous knock control algorithm results. Both control algorithms give similar torque gain of about 3% when they are optimized.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.21 no.2
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• pp.223-231
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• 1997

A variety of approaches have been investigated for the application of spark-ignition engine knock control. The control method implemented, here as "Fuzzy Control", has the advantage of not requiring the knowledge of a mathematical model of the controlled object and is more robust and flexible than conventional approaches. Knock control in this study is performed using vibration signal which is measured with accelerometer attached to the cylinder block of a 1498cc four-cylinder spark-ignition engine. The experimental results obtained with this method are compared with those obtained with a knock interval controller and with those of a conventional controller. Those results illustrate better performance in torque than knock interval controller and conventional controller.ontroller.

• Transactions of the Korean Society of Automotive Engineers
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• v.7 no.7
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• pp.32-41
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• 1999

In this study, a new knocking control algorithm was developed using the knock threshold value auto-correction algorithm. This algorithm uses the Fast Fourier Transform9FFT) method by measuring cylinder block vibration signals of a 1498 cc four-cylinder spark ignition engine. The experimental results show the proposed knock control algorithm provides improved performance compared to existing methods. The results also show that the proposed FFT algorithm provides real-time adjustment of the knock threshold value.

• Journal of the korean Society of Automotive Engineers
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• v.14 no.4
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• pp.61-67
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• 1992

The ignition timing feedback control system was studied to enhance the engine power and to reduce the fuel consumption by optimizing the spark timing. The signal of a piezo-electric vibration transducer attached to the engine block was compared with that of a pressure transducer in order to determine the knock intensity. With the result of comparison the ignition timing feedback control system which detect the knock and correct the spark timing was set up. The ignition could be more advaced with this control system than the existing system without the continuous knocking, therefore the engine torque was increased.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.10
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• pp.1473-1480
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• 2012

The Galactose-${\alpha}1$,3-galactose (${\alpha}1$,3Gal) epitope is responsible for hyperacute rejection in pig-to-human xenotransplantation. Human decay-accelerating factor (hDAF) is a cell surface regulatory protein that serves as a complement inhibitor to protect self cells from complement attack. The generation of ${\alpha}1$,3-galactosyltransferase (GGTA1) knock-out pigs expressing DAF is a necessary step for their use as organ donors for humans. In this study, we established GGTA1 knock-out cell lines expressing DAF from pig ear fibroblasts for somatic cell nuclear transfer. hDAF expression was detected in hDAF knock-in heterozygous cells, but not in normal pig cells. Expression of the GGTA1 gene was lower in the knock-in heterozygous cell line compared to the normal pig cell. Knock-in heterozygous cells afforded more effective protection against cytotoxicity with human serum than with GGTA1 knock-out heterozygous and control cells. These cell lines may be used in the production of GGTA1 knock-out and DAF expression pigs for xenotransplantation.

• The Journal of the Acoustical Society of Korea
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• v.11 no.4
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• pp.31-35
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• 1992

Cavity resonances are caused by combustion such as the rapid of pressure rise that occurs from knock in high power gasoline engines. These resonances generally occur at frequencies greater than 5KHz. Analysis of these resonances is important for knock control system design in high power gasoline engines. In this paper, in order to design knock control system for the high power gasoline engine, knock phenomena that occur in chamber were analized theoretically and resonance frequencies of knock signals were predicted. Also, experiments were performed using Soupe x-engine and non-resonance type knock sensor of Bosch co. in Germany. In the result, good agreement was obtained between theoretical prediction and experimental observation.

• Transactions of the Korean Society of Automotive Engineers
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• v.6 no.1
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• pp.205-212
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• 1998

In this study, the effects of water vapor in inlet air on combustion efficiency, general performance, knock characteristics and emission gas concentration were investig- ated through the experiments of combustion and vibration analyses, emission gas analysis by changing water vapor quantity in inlet air with temperature and humidity auto control unit. With partial vapor pressure increase, the brake torque at wide open throttle status decreased and the average ignition delay angle increased, IMEP (indicated mean effective pressured using the integral and 3rd derivatives of filtered cylinder pressure as knock intensity, which matched well with the method of frequency power spectrum of block vibration signal. Water vapor in intake air had influence on the spark knock sensitivity. With the increase of water vapor content in intake air NOx emission was decreased and HC emission was increased.

• Transactions of the Korean Society of Automotive Engineers
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• v.8 no.1
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• pp.23-31
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• 2000

Knock and misfire, kinds of abnormal combustion, are highly undesirable effect on the internal combustion engine. So, it is important to detect these avnormal combuition and control the ignition timing etc. to avoid these mal-effect factors in real engine system. In this study, the system which detects the knock and the misfire using by spark plug is presented. This system is based on the effect of modulation breakdown voltage(BDV) between the spark gaps. The voltage drop between spark plug electrodes, when an electrical breakdown is initiated, depends on the temperature and pressure in combustion chamber. So, we can detect knock and misfire that produce changes in gas temperature and pressure (consequently, its density) using by BDV signal change which carries information about the character of combustion.

• Fisheries and Aquatic Sciences
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• v.17 no.3
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• pp.377-380
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• 2014

RNA interference (RNAi)-mediated transcriptional knock-down of Crassostrea gigas big defensin 1 and 2 genes (Cg-BigDef1 and Cg-BigDef2) was investigated. The cDNA sequences of Cg-BigDef1 and Cg-BigDef2 were identical, excluding an additional fragment of 20 nucleotides in Cg-BigDef1; thus, a long double-stranded RNA (dsRNA) targeting the mRNA of Cg-BigDef2 effectively downregulated both Cg-BigDef2 and Cg-BigDef1. In addition, long dsRNA targeting green fluorescent protein (GFP) did not affect transcription of the two big defensin genes. These results suggest that the transcriptional downregulation of Cg-BigDef1 and Cg-BigDef2 was mediated by sequence-specific RNA interference (RNAi). Despite injection of long dsRNA targeting Cg-BigDef2 into only the adductor muscle, knock-down of Cg-BigDef1 and Cg-BigDef2 was observed in the adductor muscle, hemocytes, mantle, and gills, suggestive of systemic spread of RNAi in C. gigas. Furthermore, the inhibitory effect of dsRNA persisted until 72 h post-injection, indicative of a long-lasting RNAi-mediated knock-down of target genes.

• Molecules and Cells
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• v.37 no.10
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• pp.734-741
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• 2014

Histone modifications on major transcription factor target genes are one of the major regulatory mechanisms controlling adipogenesis. Plant homeodomain finger 2 (PHF2) is a Jumonji domain-containing protein and is known to demethylate the histone H3K9, a repressive gene marker. To better understand the function of PHF2 in adipocyte differentiation, we constructed stable PHF2 knock-down cells by using the mouse pre-adipocyte cell line 3T3-L1. When induced with adipogenic media, PHF2 knock-down cells showed reduced lipid accumulation compared to control cells. Differential expression using a cDNA microarray revealed significant reduction of metabolic pathway genes in the PHF2 knock-down cell line after differentiation. The reduced expression of major transcription factors and adipokines was confirmed with reverse transcription- quantitative polymerase chain reaction and Western blotting. We further performed co-immunoprecipitation analysis of PHF2 with four major adipogenic transcription factors, and we found that CCATT/enhancer binding protein (C/EBP)${\alpha}$ and C/EBP${\delta}$ physically interact with PHF2. In addition, PHF2 binding to target gene promoters was confirmed with a chromatin immunoprecipitation experiment. Finally, histone H3K9 methylation markers on the PHF2-binding sequences were increased in PHF2 knock-down cells after differentiation. Together, these results demonstrate that PHF2 histone demethylase controls adipogenic gene expression during differentiation.