• 한국연소학회지
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• 제18권1호
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• pp.7-12
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• 2013

A quasidimensional program is developed for a four stroke cycle homogeneous GDI (Gasoline Direct Injection) engine. It includes models for spray, burning rate and chemistry to predict knock and emissions. With early injection a homogeneous GDI engine goes through spark ignited, turbulent premixed combustion as in PFI (Port Fuel Injection) engines. The cylinder charge is divided into unburned and burned zone with the latter divided into multiple zones of equal mass to resolve temperature stratification. Validation is performed against measured pressure traces, NOx and CO emissions at different load and RPM conditions. Comparison is made between an empirical knock model and predictions by the chemistry model in this work.

• 대한기계학회논문집B
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• 제24권9호
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• pp.1157-1165
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• 2000

A classic examples of the abnormal combustions are the knock and misfire, which raise noxious performance and life of the engine. A heavy knock can also cause severe damages to the engine itself, which gives more reason why it must be detected and corrected. With the response of the today's requirements, we have researched the new diagnostic system which uses the breakdown voltage characteristics between electrodes of spark plug. This breakdown voltage depends on the pressure, temperature and even the shape and material of electrodes. But there is no data of breakdown voltage in case of using the spark plug as a electrodes. So, in this study, we show the breakdown voltage characteristic by pressure and temperature in constant volume bomb, which will make it possible to diagnose the engine combustion phenomenon.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2001년도 춘계학술대회논문집D
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• pp.890-895
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• 2001

Pressure resonance frequency that is caused in the combustion chamber can be interpreted to acoustic analysis. Until now the pressure resonance has been assumed and calculated to a disc type combustion chamber that neglected the combustion chamber height because the knock occurs near the TDC(top dead center). In this research FEM(fine element method) has been used to calculate the pressure resonance frequency inside the experimental engine combustion. The reduce error of the resonance frequency obtained by FEM has decreased about 50% compared to the calculation of Draper's equation. Due to the asymmetry in the shape of the combustion chamber that was neglected in Draper's equation we could find out that a new resonance frequency could be generated. To make the experimental results equal we could know that the speed of sound that satisfies Draper's equation was selected 13% higher than all the pent-roof type combustion considered.

• Molecules and Cells
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• 제41권11호
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• pp.943-952
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• 2018

The discovery and mechanistic understanding of target-specific genome engineering technologies has led to extremely effective and specific genome editing in higher organisms. Target-specific genetic modification technology is expected to have a leading position in future gene therapy development, and has a ripple effect on various basic and applied studies. However, several problems remain and hinder efficient and specific editing of target genomic loci. The issues are particularly critical in precise targeted insertion of external DNA sequences into genomes. Here, we discuss some recent efforts to overcome such problems and present a perspective of future genome editing technologies.

• 한국연소학회:학술대회논문집
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• 한국연소학회 2012년도 제44회 KOSCO SYMPOSIUM 초록집
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• pp.101-104
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• 2012

In this work, in-cylinder pressure measurements and high-speed direct imaging of the flame were performed in an optically accessible single cylinder diesel engine with premixed charge compression ignition combustion and a narrow injection angle. The results show that the frequency ranges of pressure ringing were 8.35 to 9 kHz and 12..2 to 13.1 kHz. The frequencies of the flame movement were shown as 8.7 kHz and 13 kHz. It was found that there is a direct relationship between the pressure ringing and the flame movement.

• Journal of Microbiology
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• 제44권3호
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• pp.320-326
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• 2006

Pasteurella multocida is an important veterinary and opportunistic human pathogen. In particular, strains of P. multocida serogroup D cause progressive atrophic rhinitis, and produce a potent, intracellular, mitogenic toxin known as P. multocida toxin (PMT), which is encoded by the toxA gene. To further investigate the toxigenic and pathogenic effects of PMT, a toxA-deleted mutant was developed by homologous gene recombination. When administrated to mice, the toxigenicity of the toxA mutant P. multocida was drastically reduced, suggesting that the PMT constributes the major part of the toxigenicity of P, multocida. Similar results were obtained in a subsequent experiment, while high mortalities were observed when toxA(+) P. multocida bacterial culture or culture Iysate were administrated. Mice immunized with toxA(-) P. multocida were not protected (none survived) following challenge with toxA(+) P. multocida or bacterial culture Iysate (toxin). These results suggest that the toxigenicity of P. multocida is mainly derived from PMT.

• BMB Reports
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• 제52권8호
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• pp.475-481
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• 2019

The evolution of genome editing technology based on CRISPR (clustered regularly interspaced short palindromic repeats) system has led to a paradigm shift in biological research. CRISPR/Cas9-guide RNA complexes enable rapid and efficient genome editing in mammalian cells. This system induces double-stranded DNA breaks (DSBs) at target sites and most DNA breakages induce mutations as small insertions or deletions (indels) by non-homologous end joining (NHEJ) repair pathway. However, for more precise correction as knock-in or replacement of DNA base pairs, using the homology-directed repair (HDR) pathway is essential. Until now, many trials have greatly enhanced knock-in or substitution efficiency by increasing HDR efficiency, or newly developed methods such as Base Editors (BEs). However, accuracy remains unsatisfactory. In this review, we summarize studies to overcome the limitations of HDR using the CRISPR system and discuss future direction.

• Steel and Composite Structures
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• 제30권4호
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• pp.305-313
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• 2019

Creating different cutout shapes in order to make doors and windows, reduce the structural weight or implement various mechanisms increases the likelihood of buckling in thin-walled structures. In this study, the effect of cutout shape and geometric imperfection (GI) is simultaneously investigated on the critical buckling load and knock-down factor (KDF) of composite cylindrical shells. The GI is modeled using single perturbation load approach (SPLA). First, in order to assess the finite element model, the critical buckling load of a composite shell without cutout obtained by SPLA is compared with the experimental results available in the literature. Then, the effect of different shapes of cutout such as circular, elliptic and square, and perturbation load imperfection (PLI) is investigated on the buckling behavior of cylindrical shells. Results show that the critical buckling load of a shell without cutout decreases by increasing the PLI, whereas increasing the PLI does not have a great impact on the critical buckling load in the presence of cutout imperfection. Increasing the cutout area reduces the effect of the PLI, which results in an increase in the KDF.

• 한국광과학회:학술대회논문집
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• 한국광과학회 2002년도 정기총회 및 제9회 광과학 학술심포지움 논문초록
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• pp.47-47
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• 2002

• 복식문화학회:학술대회논문집
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• 복식문화학회 2001년도 International Costume Culture Conference
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• pp.53-54
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• 2001