• Korean Journal of Animal Reproduction
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• v.17 no.4
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• pp.347-356
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• 1994

A simpled and sensitive microplate enzyme immunoassay(EIA) was developed for the determination of progesterone concentration in serum, based on progesterone monoclonal antibody as anti-progesterone, horseradish peroxidase(HRP) as enzyme-label and tetramethylbenzidine(TMB) as substrate. The assay has a sensitivity of 5 pg-120pg/well and intra- and inter-assay coefficients of variation for progesterone standard curve (1.0ng~10.0ng/ml) were ranged 2.5~9.9% and 1.7.8.0%, respectively, determination coefficient of the regressio equation of our standard curve(R2=0.990$\pm$0.007) were high, and this is the same level as that of commercial kit(Hormonost Bio-Lab, Germany, R2=0.98~0.99). The progesterone concentration of serum determined by both kits (Work & Bio-Lab) were significantly correlated (r=0.95, P<0.01) although a little higher value were resulted in our kit than that of commercial kit. It generally is these results indicated that the microplate-EIA can be cused for the determination of progesterone in serum, as well as, for the determination of the early pregnancy diagnosis.

• Proceedings of the Korean Information Science Society Conference
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• 2010.06b
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• pp.192-196
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• 2010

본 논문에서는 증강현실 기술에 대하여 살펴보고, 워싱턴 대학교에서 개발한 프로그래밍 라이브러리의 형태인 ARToolKit과 오스트리아, 그라츠 대학교에서 개발한 ARToolKit PLUS에 대하여 각 툴킷의 프로세스 및 성능에 대하여 분석하고자 한다. 가상현실 기술은 실 세계와 가상 세계를 실시간으로 혼합하여 사용자에게 제공함으로써 정보 사용의 효율성과 효과성을 극대화하는 기술이며, 이는 향후 IT기술 전분야의 발전과 변화에 많은 영향을 줄 '주목해야 할 기술'이다. 가상현실 기술이 사용자들에게 쉽게 받아들여지고, 보다 적극적으로 널리 활용되기 위해서는 기술적 한계의 극복과 가상현실에 특화된 사용자 상호작용 기술 개발 및 응용 서비스 창출 등이 요구된다.

• Korean Journal of Veterinary Research
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• v.31 no.2
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• pp.223-228
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• 1991

Most progesterone enzyme immunoassays(EIA) are used liquid-phase double-antibody or single-antibody seperation. These methods consume considerable time and reagents because of the requirements for several washing and centrifugation steps involving the reactants. Because of these several problems, we were prompted to develop an effective enzyme-linked immunosorbent assay(ELISA) system that would be equal or superior to RIA for assay of progesterone. The results were obtained as follows. 1. Cross reaction of the progesterone antiserum with other steroids determined was shown with progesterone(100%), $11{\alpha}$-deoxycorti-costerone(2.271%), but the other steroids were shown below 0.9%. 2. Standard curve for progesterone ELISA was shown available difference according to progesterone concentration from 0 to 1,000pg/ml. 3. The lower limit of sensitivity was 0.2pg/well 4. Progesterone concentration was 1.6ng/ml for before parturition, and that was below 0.5ng/ml for after parturition. This development enzyme-linked immunosorbent assay for progesterone can be detected pregnancy diagnosis in cattle, and also applicable 10 research on physiological function including such as reproductive disorders.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.2 no.1
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• pp.11-15
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• 2009

In this study, we have developed the SMPS(Switched-mode power supply) power module for the medical kit. It is used the medical kit for improved supplying power better than the existing power module in performance, safety and reliability. The developed SMPS(Switched-mode power supply) is composed of the three fundamental electronic circuits, first one is for converting AC power to DC power, second one is for converting to high frequency, and the other is for absorbing noise frequency and preventing malfunction. It is possible for developed SMPS module to enlarge applications for PC, home appliances, switchboard as well as medical instruments.

• The Bulletin of The Korean Astronomical Society
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• v.46 no.1
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• pp.38.1-38.1
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• 2021

본 발표에서는 Transformable Reflective Telescope(TRT kit)의 새로운 버전을 소개한다. TRT kit는 기본형인 뉴턴식 반사망원경에서 부경 교체를 통해 카세그레인식, 그레고리식으로 간단하게 변형 할 수 있는 광학 실험장치이다. 본 장치는 주로 망원경 교육이나 광학계 개발에 필요한 실험에 활용된다. 모듈화 설계를 통해서 여러 종류의 광학계를 쉽게 탈착하여 다양한 실험을 할 수 있다. 광기계부는 정밀하게 제작된 알루미늄 프로파일과 Isogrid구조를 채택하여 경량화 구조로 설계되었다. 이러한 경량부품들을 통해 이전 버전보다 50~70%의 중량 감소율을 달성하였다. 유한요소해석 결과 경량화된 뉴턴식 TRT kit는 이전 버전과 비교해서 자체 하중에 의한 최대 구조 변형이 0.11mm에서 0.023mm로 감소하였다. 부경 지지대 설계에는 자체 하중으로 인한 변형을 최소화하기 위해 트러스 (Truss) 구조가 도입되었다. 부경부의 자체 하중으로 인한 변형은 기존의 80㎛에서 21㎛로 감소하였다. 또한, 십자 레이저 정렬 장치가 추가되어 뉴턴식과 카세그레인식에서 공차 1.5' 이내로 광학계 정렬이 가능하다.

• Toxicological Research
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• v.20 no.2
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• pp.101-108
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• 2004

The different social reflections about the benefits and the potential risks of genetically modified (GM) crops have evolved with .different reactions in different countries. Many countries including Korea are working toward setting down new guidelines. Korea requires companies to label all food that contains more than 3% GM ingredients. One of the rapid and convenient detection methods of GM ingredients is amplification of the introduced DNAs by polymerase chain reaction (PCR). Many PCR kits for this purpose are commercially available. The objective of this study was to evaluate performance of commercialized GM crop detection kits. The results showed that 6 out of 15 kits tested did not meet the requirements even purposed by the manufacturers themselves in terms of stability, reproducibility, and detection limits, suggesting a potential quality control problem in their design stage or production line. The evaluation also suggests that, although the duplex and triplex detection kits allowed unambiguous detection in a single PCR reaction, the monoplex detection kits were the most sensitive to the detection of GM ingredients. The detection limits also differ between soybean and corn. Results from this study will be useful in the development of sound qualitative tracking systems of GM ingredients for monitoring throughout the cultivation of GM crops, their trans-boundary movement, and food production using GM grains as well as for complying with government guidelines associated with GM crops.

• Journal of Animal Science and Technology
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• v.59 no.12
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• pp.25.1-25.7
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• 2017

Background: Rabbit breeding with high performance imported ones would be of benefit for genetic diversity and improvement of performance in domestic rabbit breeds. The rearing of more productive rabbit breeds could be pathway to improve the productivity and reduce the production cost. Maternal nutritional status exert a great influence on reproductive functions of does, which may expand from conception, through gestation and parturition and development of kits to puberty. Methods: Four breeds of rabbit were evaluated for their parturition, weaning and pubertal differences among the rabbit population in Ibadan, Nigeria. The breed consist of Fauve De Bourgogne (FDB), Chinchilla (CHA), British Spot (BS) and New Zealand White (NZW) rabbits. A total of 60 bucks and 360 does consisting of 15 bucks and 90 does per breed were mated in 6 mating cycles, three each of natural mating and artificial insemination. All does were synchronized for estrus with 20 IU pregnant mare serum gonadotropin 48 h prior to mating. The does after parturition were assessed for milk yield (g) and kit survival rate (%) till weaning, weight changes of kits from birth to puberty. At puberty, the pubertal age (days) and weight (g) of the offspring were assessed. Result: Results obtained reveals that British Spot doe had highest milk yield among the breeds which significantly increased growth of kit and weight at weaning in British Spot rabbits. Survival rates of Chinchilla kits were significantly (p < 0.05) higher than Fauve de Bourgogne, British Spot and New Zealand White kits. Puberty attainment of the rabbits indicates that British spot does and Fauve de Bourgogne bucks are early maturing. Conclusion: Chinchilla shows high kit survivability and British spot has highest milk yield among the four breeds of rabbit.

• Korean Journal of Veterinary Service
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• v.38 no.1
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• pp.37-42
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• 2015

The aim of the present study was to compare the non-invasive methods for the diagnosis of H. felis with HpSA kit-based detection method and H. felis-specific PCR assay with dog's stool samples without sacrifice. Male Beagle dogs (n=6) were infected with H. felis ATCC 49179 ($1.0{\times}10^9CFU/dog$) by intra-gastric inoculation two times at 3-day intervals, and the stool specimens of dogs were collected 1, 3, 5, 7, 14, 21 days after infection to submit to HpSA test and H. felis-specific PCR. As the results, the sensitivity of the HpSA and the PCR analysis was 50.0%, 83.3% respectively. Although HpSA test is less sensitive, it could be used for rapid, cheap and easy screening assay for H. felis infection in dog and cats. We suggest that the H. pylori stool antigen kit, HpSA, is useful and effective for monitoring H. felis infection. If HpSA test would be made with H. felis antibodies in the future, its sensitivity could be increased. Also, PCR assay could be successfully used to detect the H. felis in stools. Applying the H. pylori stool antigen kit and PCR assay may be the recommended non-invasive strategy to identify H. felis in dog and cats.

• Journal of Bio-Environment Control
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• v.27 no.1
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• pp.86-93
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• 2018

This present study was conducted to develop the domestic cultivation kit using water celery(Oenanthe Stolonifera DC.) seed. As the result of germination rates in 3 type inbred lines, the IT 232354 had the highest initial germination rate and final germination rate, and was selected as the inbred line to be used in the cultivation kit. The development of the domestic cultivation kit was carried out using the IT 232354 seeds. It was possible to cultivate up to 3 times harvest using the same root in this cultivation kit, though the growth decreased rapidly in the $4^{th}$ cultivation. As a result of investigating the effects of the type of nutrient solution on growth of water celery, the overall growth was the lowest in the nutrient solution for Oenanthe Stolonifera DC.(N.S.D.). The shoot growth was similar to that of nutrient solution for leaves and stem vegetables (N.S.L.S.) and amino acid fermentation by-product liquid (A.F.B.L.), however in the root growth, the N.S.L.S. was more effective than A.F.B.L. When it was harvested 4 times consecutively after 1 time of planting, the last survival ratio of A.F.B.L. was 100% while their ratios were 96.4% and 49.8% each for N.S.L.S. and N.S.D. For the growth by cultivation kit type, the hole type cultivation kit increased slightly compared to the 3 hole type cultivation kit in the $1^{st}$ and $2^{nd}$ harvest, but there was no difference in the $3^{rd}$ and $4^{th}$ harvest. Total yield of one cultivation kits showed the 3 hole type cultivation kit is much higher than the 2 hole type cultivation kit. According to the results of this experiment, it is possible to harvest three times by planting one times if it was cultivated using N.S.L.S. and A.F.B.L. in the 3 hole type cultivation kit.

• Journal of the Korea Society of Computer and Information
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• v.19 no.10
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• pp.91-97
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• 2014

In this paper, we design and implement a mobile game based on windows phone platform. Visual Studio and the Windows Mobile software development kit(SDK) and developer tool kits (DTK) make it possible to create software for the Windows Mobile platform in Visual C#. Also The XNA Framework is the set of .NET libraries that developers will code against to build games. XNA Framework support PC and XBOX and a ZUNE HD multiplex platform environment and 2D and 3D games provides the necessary functions to a development. XNA Framework is to make it easier to develop games because the codes which are succeeded creates with automatic, implements a game development relation resource and the game logic. The key idea of our game is that we use shooting and ranking system to raise fun.