• Title/Summary/Keyword: Kit development

Search Result 482, Processing Time 0.031 seconds

Development of cellulose nano beads based a rapid detection kit to detect staphylococcal enterotoxin B

  • Kim, Giyoung;Yoo, Jinyoung;Park, Saetbyeol
    • Korean Journal of Agricultural Science
    • /
    • v.46 no.3
    • /
    • pp.549-557
    • /
    • 2019
  • Staphylococcal enterotoxin is a very common cause of food poisoning. Conventional detection methods for the toxin including enzyme-linked immunosorbent assays (ELISAs), chemiluminescence (ECL), and polymerase chain reaction (PCR) assays require a lot of time, efforts, and expert technicians. Lateral flow strip kits have shown great potential for the rapid detection of foodborne pathogens. The lateral flow strip kit is widely used in clinical settings because it is easy to use, fast, and cost effective. A typical lateral flow strip kit uses colloidal gold to generate a visual signal. However, the lateral flow strip kit based on colloidal gold has limited sensitivity to fulfill food safety regulation requirements. This study was performed to develop a rapid test kit for pathogenic staphylococcal enterotoxin B (SEB) in food samples. The rapid detection kit was fabricated based on a nitrocellulose lateral-flow strip. Cellulose nano beads and SEB antibodies were used as the tag and receptor, respectively, to improve the detection performance. Manually spotted SEB antibody and anti-rabbit antibody on the surface of the nitrocellulose membrane were used as test and control spots, respectively. The feasibility of the rapid test kit to detect SEB in samples was evaluated. The sensitivity of the kit was 10 ng/mL SEB spiked in PBS. Additionally, the rapid test kit could detect 1 ng/mL of SEB in chicken meat extract.

Development and Validation Study of Biological Agent Detection Kit (생물학작용제 검출 키트 개발 및 성능시험 연구)

  • Joe, Hae Eun
    • Journal of the Korea Institute of Military Science and Technology
    • /
    • v.22 no.4
    • /
    • pp.575-580
    • /
    • 2019
  • In biological warfare, it is important to identify biological agents for proper treatment. We focused on developing a real-time RT-PCR kit that can detect multiple species of biological agents. AccuPower(R) Biothreat Real-Time RT-PCR Kit(v3.0) could detect Bacillus anthracis, Yersinia pestis, Vibrio cholerae, Francisella tularensis, Salmonella typhi, Rickettsia prowazekii, Variola virus, Hantaan virus, Yellow fever virus, Brucella spp., Shigella dysenteriae in a single reaction. The results showed that the kit was verified to be able to detect at least 0.005 ng of nucleotide and 10,000 CFU/ml of bacteria. Therefore, the kit is expected to be used as a rapid and sensitive detection kit for 11 species of biological agents within 2 hours.

Development of an Easy-assemble Arduino Car Kit for Practice (쉽게 조립 가능한 실습용 아두이노 자동차 키트 개발 사례)

  • Lee, Eun-Sang
    • Journal of the Korea Convergence Society
    • /
    • v.13 no.2
    • /
    • pp.31-37
    • /
    • 2022
  • The objective of this study was to present an example of the development of an Arduino car kit for practice. First, problems in the existing Arduino car kit were analyzed and various prototypes were developed that reflected the improvement plan. The developed kit was applied to the education field to identify problems and improvements, following which it was corrected and supplemented for use as an Arduino car kit for final practice. The Arduino car kit can be used for various experiments and practices related to an Arduino car by using a combination of two car bodies consisting of an upper plate and a lower plate. When a method to couple the upper and lower plates was used, the car body could be easily and quickly configured without the need for bolts or nuts. The developed kit involves a simple and easy assembly method, and hence, the time required for assembling a car body is considerably short. Accordingly, it is expected to be widely used as a kit that can directly experience programming education using a car.

Tests of a Guidance Kit for Air-to-Surface Bomb (공대지 폭탄용 유도키트 시험)

  • Lee, Inwon;Lee, Kidu;Park, Youngkuen;Lim, Sangsoo;Baek, Seungwoock;Lee, Daeyearl
    • Journal of the Korean Society for Aeronautical & Space Sciences
    • /
    • v.41 no.4
    • /
    • pp.314-318
    • /
    • 2013
  • Tests and evaluations following the U.S. MIL-HDBK/STANDARD were successfully conducted to assure the performance of the air-to-surface guidance kit which was developed first in Korea. Various ground tests confirmed the operation capability and reliability of the guidance kit, and flight tests proved very good mid-range gliding performance and accuracy of the gliding bomb which was a general purpose bomb with the guidance kit.

An Example of Engineering Education through SE-based Development of Underwater-robot Kit Model (수중로봇키트 모형의 SE기반 개발을 통한 공학교육 사례)

  • Kim, Hyun-Sik;Kang, Hyung-Joo;Man, Dong-Woo
    • Journal of Engineering Education Research
    • /
    • v.15 no.3
    • /
    • pp.40-46
    • /
    • 2012
  • This paper deals with an example of the engineering education through the SE-based development of an underwater-robot kit model. It has a purpose of establishing the example of a SE-based development by undergraduate through executing the requirement and functional analysis, hardware and software design, manufacturing, test and evaluation for developing the model. In addition, it also has a purpose of establishing the basis of the development of an optimized underwater-robot kit through understanding the weight and buoyancy characteristics and the actuation and propulsion characteristics of the developed model.

Development of a Remotely Operated Aerial Robot-kit based on the Balloon (풍선기반 원격조종 공중로봇키트 개발)

  • Kim, Hyun-Sik
    • The Journal of Korea Robotics Society
    • /
    • v.7 no.3
    • /
    • pp.216-221
    • /
    • 2012
  • Recently, although the need of marine robots being raised in extreme areas, the basis is very deficient. Fortunately, as the robot competition is vitalizing and the need of the robot education is increasing, it is desirable to establish the basis of the R&D and industrialization of marine robots and to train professionals through the development and diffusion of marine robot kits. However, in conventional case, there is no remotely operated aerial robot-kit based on the balloon for the marine robot competition, which has the abilities of the airborne locomotion and obstacle avoidance. To solve this problem, an aerial robot-kit which has the abilities of the airborne locomotion and remote control, is developed. To verify the performance of the developed kit, test and evaluation such as surge, yaw and pitch is performed. The test and evaluation results show that the possibility of the real applications of the developed kit.

Development of Onboard Power Supply Device for Precision Guided Kit (정밀유도 비행체 탑재용 전원공급장치 개발)

  • Park, Deok-Bae;Kim, Hyung-Shin
    • The Transactions of the Korean Institute of Electrical Engineers P
    • /
    • v.57 no.3
    • /
    • pp.209-214
    • /
    • 2008
  • A precision guided kit is a GPS/INS guided range extension system to improve the accuracy and availability of existing dumb bombs. The PSD(power supply device) installed in the GPS guided kit supplies electrical power to all electrical loads in the kit. In this paper, design process and test results of the PSD have been presented. Additionally, the performance results of the PSD during actual ground and flight tests have been described.

Development of a Kit for Realtime transmission of data acquired by Earthquake accelerometers (지진가속도 계측기의 실시간 자료 전송 Kit 개발)

  • Heo, Jeongpil;Kim, Kiseog;Lim, Inseub;Jin, Jocheol
    • Journal of the Society of Disaster Information
    • /
    • v.10 no.2
    • /
    • pp.254-262
    • /
    • 2014
  • KISStool is developed and established by KIGAM, in order to link and share the earthquake data easily with institutes related, this study includes the development of a KISStool program format and protocol supporting Kit. The Kit has functions such as transforming and sending a seismic data. The functions of the kit are implemented in modules, which are running on the realtime basis. The firmware is embedded into the Kit, which transforms the seismic data into the formats, miniSEED and QSCD20. These data are sent to KISStool program. We have confirmed the results of the data transforming, transmissions and storage functions are conducted normally, the speed of transmission shows same level when comparing with a standard system. We expect that the firmware developed in this study will have high usability not only for linking with KISStool program through being embedded into incompatible digitizers, but also for improving performance of older ones.

Development of Multi-Residue Methods for Carbamate Pesticides by the Enzyme Inhibition Test (효소 저해법을 이용한 Carbamate계 농약의 다성분 잔류분석법 개발)

  • Kim, Jung-Ho
    • Journal of Environmental Science International
    • /
    • v.17 no.12
    • /
    • pp.1325-1330
    • /
    • 2008
  • This study was carried out with the detection for multiresidue of the carbamate pesticide such as carbaryl and cabofuran by enzyme-inhibition method. The check time for determination of acetylcholinesterase(AChE) activity was selected at 60 sec. The AChE activity in chicken brain determined by the Ellman's method was $162{\mu}$mol/min/g protein. $I_{50}$ for AChE by carbamate pesticide with wet kit was 0.169mg/L of carbaryl and 0.089mg/L of cabofuran, respectively. The incubation time for enzyme kit with substrate kit was 30min for determination of AChE activity. Enzyme kit with substrate kit was stable at $4^{\circ}C\;and\;25^{\circ}C$ for 5 days. Limit detection concentration of carbaryl with dry kit for AChE was 0.05mg/L. The dry kit such as wet kit applied Enzyme-Inhibition(EI) method with AChE was confirmed the multi residue method to detect the carbamate pesticides.

Blood Pressure control application using Android Open Accessory Development Kit (Android Open Accessory Development Kit를 이용한 혈압계 제어 application)

  • Park, Hee-Jung;Jung, Hae-In;Lee, Young-Jae;Lee, Jae-Ho;Lim, Min-Gyu;Lee, Jeong-Whan
    • Proceedings of the Korean Society of Computer Information Conference
    • /
    • 2012.07a
    • /
    • pp.409-410
    • /
    • 2012
  • 본 논문에서는 스마트케어 서비스를 위해, Android 운영체제를 기반으로 하는 Device에서 ADK를 이용해 혈압계를 제어하는 방법을 제안한다. 이 방법은 소프트웨어와 하드웨어를 결합한 kit인 ADK를 사용하여 Hardware를 잘 모르는 사람들도 Android에서 Hardware의 GPIO에 좀 더 쉽게 접근하고 제어할 수 있어 보다 다양한 기능을 구현할 수 있다. 또한 이 방법으로 얻은 혈압계 데이터를 Database에 저장하여 데이터통신망을 통해 전문의에게 Feedback을 받음으로써 굳이 병원을 방문하지 않더라도 개인의 건강을 보다 편리하게 관리할 수 있어 한 단계 수준 높은 진단을 받을 수 있다.

  • PDF