• 제목/요약/키워드: Kit

검색결과 2,366건 처리시간 0.028초

Progesterone EIA-kit에 의한 유우(乳牛)의 임신진단(姙娠診斷)에 관한 연구(硏究) (Studies on the early diagnosis of pregnancy of dairy cows by EIA-kit of progesterone in milk)

  • 김민규;신현주;이만휘;이명헌;김상근
    • 대한수의학회지
    • /
    • 제30권1호
    • /
    • pp.35-40
    • /
    • 1990
  • This study was carried out to evaluate khe accuracy of the early diagnosis of pregnancy in dairy cows using EIK-kit of progesterone in milk and to compare the progesterone concentrations in milk with those assayed by radioimmunoassay. 1. The progesterone concentrations of the pregnant cows($2.07{\pm}0.54ng/ml$) were significantly higher than those of non-pregnant cows($1.04{\pm}0.19ng/ml$), and thereafter began to inerease and maintained high levels. 2. During 20 to 22 days after artificial insemination, the accuracy of pregnancy diagnosis from EIA-kit of progesterone were 93.7% for non-pregnant cows, and 91.6% for pregnant cows. 3. During 20 to 22 days after artificial insemination, the accuracy of pregnancy diagnosis from milk progesterone concentrations were 100% for non-pregnant cows (<2.7ng/ml), and 92.9% for pregnant cows($${\geq_-}3.2ng/ml$$). The average overall accuracy of prediction for pregnant and non-pregnant cows were 96.5%. 4. Accordingly, the pregnancy diagnosis from EIA-kit of progesterone is thought to be recommendable because this early diagnostic means are simple with accurate result.

  • PDF

Development of Rapid Immune-gold Strip Kit for On-Site Diagnosis of Tomato spotted wilt virus

  • Yoon, Ju-Yeon;Choi, Gug-Seoun;Cho, In-Sook;Choi, Seung-Kook
    • 식물병연구
    • /
    • 제20권1호
    • /
    • pp.15-20
    • /
    • 2014
  • A rapid, user-friendly and simple immune-chromatographic dipstick kit named 'rapid immune-gold strip' (RIGS) kit was developed in a novel single strip format to detect on-site detection of Tomato spotted wilt virus (TSWV). Immunoglobulin G (IgG) from polyclonal antisera raised in rabbits against TSWV was purified through protein-A affinity chromatography and then the purified TSWV-IgG was conjugated to colloidal gold nano-particles which served as a test line on nitrocellulose membrane. Protein A that non-specifically binds to TSWV antibody was used as a control line on the same strip. The diagnosis process with the TSWV-RIGS involves simply grinding the suspect plant sample in a bag that contains the extraction buffer and inserting the strip the bag. Results can be seen in 2-5 minutes. The flow of the complexes of gold particles coated with TSWV-IgG and a crude sap from TSWV-infected pepper, tobacco and tomato plants resulted in intensive color formed on the test lines proportional to the concentrations of TSWV. The RIGS-TSWV kit did not show any cross-reactions against other tomato-infecting viruses unrelated to TSWV. These results indicate that the TSWV-RIGS kit is highly sensitive and is not required for laboratory training and experience prior to testing. The TSWV-RIGS kit is suitable for on-site detection of suspect TSWV-infected plants as well as for laboratory diagnosis.

외식업체 밀키트(Meal Kit)상품에 대한 1인가구의 주관적 인식유형 분석연구 (Subjectivity Type Analysis Study of Single-person Households on Meal Kit Products of Food Service Industry)

  • 김찬우;이강연
    • 한국콘텐츠학회논문지
    • /
    • 제20권4호
    • /
    • pp.406-415
    • /
    • 2020
  • 본 연구에서는 사회구조적으로 변화하는 인구구조에 따라 외식기업이 개발 및 출시하는 밀 키트(Meal Kit) 상품이 주요 수요층인 1인 가구에 대해 주관적 의견이나 인식 구조를 분석하여 유형별로 추출이 가능한 Q방법론을 적용하여 그 함의를 분석하였다. 본 연구의 주관적 인식 유형 분석 결과 총 4가지의 유형이 도출되었으며, 유형은 다음과 같이 각각 그 특이성에 따라 명명하였다. 제 1유형(N=6) : 외형평가 구매 유형(Appearance Assessment Purchase Type), 제 2유형(N=6) : 가격비교 구매 유형(Price Comparison Purchase Type), 제 3유형(N=10) : 브랜드 신뢰 구매 유형(Brand Trust Purchase Type), 제 4유형(N=2) : 간편조리 구매 유형(Easy Cooking Purchase Type) 으로 각 각 독특한 특징의 유형으로 분석되었다. 본 연구에서는 밀키트 제품이 주요 수요층인 1인 가구에 대해 주관적 의견이나 인식 구조를 분석하여 유형별 분석을 통해 추후 외식업체의 1인가구를 위한 외식상품개발 및 출시에 대한 방향성과 그 개선책에 대해 제안하며, 분석결과를 바탕으로 학문적 시사점을 제공하는데 목적을 두고자 한다.

면역리포좀을 이용한 계란에서의 살모넬라 분석과 시판 간이키트와의 비교 (Analysis of Salmonella Species from Eggs Using Immunoliposomes and Comparison with a Commercial Test Kit)

  • 신원선;김윤숙;이준수;김명희
    • 한국축산식품학회지
    • /
    • 제29권4호
    • /
    • pp.533-538
    • /
    • 2009
  • 보다 우수한 살모넬라 진단법을 개발하고자, 면역리포좀을 이용하여 계란에서의 살모넬라를 분석하는 새로운 진단법과 시판 간이키트를 사용한 살모넬라 분석과의 결과를 비교하였다. 면역리포좀을 이용하여 난각을 분석한 결과, 36개의 계란 시료 중 1건에서 살모넬라 양성 결과가 나타났으며 시판 간이키트를 이용하여 분석한 결과, 36개의 계란 시료 중 6건에서 살모넬라 양성 결과가 나타났다. 총 7건의 양성 결과는 생화학적 동정에 의해서 살모넬라균이 아닌 것으로 확인되었다. 난황과 난백으로 이루어진 내용물의 경우, 두 분석법에 의하여 살모넬라 균이 전혀 검출되지 않았으며 이는 배지 배양법과 일치하는 결과였다. 면역리포좀을 이용한 진단법은 시판 간이키트에 비해 민감도가 높고 분석에 소요되는 총 시간이 24시간 단축되는 우수성을 보였다. 난각에서는 Acinetobacter baumanni, Chryseomonas luteola, Enterobacter cloacae, Escherichia coli, Escherichia hermannii, Klebsiella pneumonia, Pantoea spp., Pasteurella pneumotropica와 같은 세균이 분리되었는데, A. baumanni와 P. pneumotropica를 제외한 대부분의 세균들은 계란의 전처리 공정 중에 빈번하게 분리되는 균들로 확인되었다.

Al-Si 합금의 양극산화거동 및 규소화합물 제어 (Anodizing Behavior and Silicides Control in Al-Si Alloy System)

  • 박종문;김주석;김재권;김수림;박노진;오명훈
    • 열처리공학회지
    • /
    • 제31권1호
    • /
    • pp.6-11
    • /
    • 2018
  • The anodic oxidation behavior of Si-containing aluminum alloy for diecasting was investigated. Especially, the property changes during anodization both on aluminum 1050 and 9 weight percentage silicon containing aluminum (Al-9Si) alloys were analyzed by the static current test. In order to fabricate a uniform anodic oxidation film by effect of Al-Si compound, nitric acid containing hydrofluoric acid had been used as a desmutter for aluminum alloy after alkaline etching. It was found that the level of voltage of Al-9Si alloy during the static current test was almost as double as higher than aluminum 1050 through anodization. By adding hydrofluoric acid in the nitric acid electrolyte, the silicon compound on the surface was removed, and the optimum amount of added hydrofluoric acid could be derived. It was also observed that the size of silicon compound formed on the surface could be refined by heat treatment at $500^{\circ}C$ and followed water quenching.

Effects of different surface finishing procedures on the change in surface roughness and color of a polymer infiltrated ceramic network material

  • Ozarslan, Mehmet Mustafa;Buyukaplan, Ulviye Sbnem;Barutcigil, Cagtay;Arslan, Merve;Tuker, Nurullah;Barutcigil, Kubilay
    • The Journal of Advanced Prosthodontics
    • /
    • 제8권1호
    • /
    • pp.16-20
    • /
    • 2016
  • PURPOSE. Polymer infiltrated ceramic network (PICN) materials, also called hybrid ceramics, are new materials in dental market. The manufacturer of the PICN material VITA Enamic suggests 3 different finishing procedures for this new material. In the present study, surface roughness and color differences caused from different finishing procedures of VITA Enamic were investigated. MATERIALS AND METHODS. 120 specimens were prepared in dimensions $2{\times}10{\times}12mm$ from VITA Enamic hybrid ceramic blocks with 'high translucency' and 'translucency 2M2' shades. The specimens were divided into 8 groups. For each group, different finishing procedures suggested by the manufacturer were performed. Surface roughness values were determined by a tactile portable profilometer. Color changes were evaluated using a clinical spectrophotometer. The data were analyzed using one-way ANOVA and Tukey's post-hoc comparison. The significance level was set at ${\alpha}=0.05$. RESULTS. The roughest surfaces were observed in Glaze Groups. Their surface roughness values were similar to that of the control group. Clinical Kit and Technical Kit groups did not show a statistically significant difference regarding surface roughness (P>.05). The largest color difference regarding ${\Delta}E_{00}$ was observed in Clinical Kit finishing groups. There were also statistically significant color changes between the groups (P<.05). However, all the groups showed clinically acceptable color change (${\Delta}E_{00}$<2.25) except Clinical Kit Groups (${\Delta}E_{00}$>2.25). CONCLUSION. Within the limitations of the present study, it may be suggested that finishing the VITA Enamic restorations by Technical Kit instead of Glaze and Clinical Kit gives better clinical performance in regard to surface roughness and shade matching.

수중형 자율운항 해양로봇키트 개발 (Development of Underwater-type Autonomous Marine Robot-kit)

  • 김현식;강형주;함연재;박승수
    • 한국지능시스템학회논문지
    • /
    • 제22권3호
    • /
    • pp.312-318
    • /
    • 2012
  • 최근에는, 극한 분야에서 해양로봇의 필요성이 제기되고 있으나, 그 기반이 매우 부족한 실정이다. 다행히 로봇경진대회가 활성화되고 로봇교육에 대한 수요가 증가하는 추세이므로, 해양로봇키트의 개발/보급을 통하여 해양로봇 연구개발/산업화 기반을 마련하고 전문인력을 양성하는 것이 바람직하다. 그런데, 기존에는 수중이동 및 표적 탐지/회피가 가능한 해양로봇경진대회용 수중형 자율운항 해양로봇키트가 없었다. 이 문제를 해결하기 위해서 수중이동성, 수중방수성 및 무게조절성이 우수한 보급형 해양로봇키트가 개발되었다. 개발된 키트의 성능 검증을 위해서 Surge, Pitch, Yaw, 장애물회피 등의 시험 평가가 수행되었다. 시험평가 결과는 개발된 키트의 실제 적용 가능성을 보여준다.

PC 기판위에 성막한 IZO/Ag/IZO 박막의 특성과 이를 이용하여 제작한 플렉시블 유기발광다이오드의 특성 분석 (Characteristics of flexible IZO/Ag/IZO anode on PC substrate for flexible organic light emitting diodes)

  • 조성우;정진아;배정혁;문종민;최광혁;김한기
    • 한국전기전자재료학회:학술대회논문집
    • /
    • 한국전기전자재료학회 2007년도 하계학술대회 논문집 Vol.8
    • /
    • pp.381-382
    • /
    • 2007
  • IZO/Ag/IZO (IAI) anode films for flexible organic light emitting diodes (OLEDs) were grown on PC (polycarbonate) substrate using DC sputter (IZO) and thermal evaporator (Ag) systems as a function of Ag thickness. To investigate electrical and optical properties of IAI stacked films, 4-point probe and UV/Vis spectrometer were used, respectively. From a IAI stacked film with 12nm-thick Ag, sheet resistance of $6.9\;{\Omega}/{\square}$ and transmittance of above 82 % at a range of 500-550 nm wavelength were obtained. In addition, structural and surface properties of IAI stacked films were analyzed by XRD (X-ray diffraction) and SEM (scanning electron microscopy), respectively. Moreover, IAI stacked films showed dramatically improved mechanical properties when subjected to bending both as a function of number of cycles to a fixed radius. Finally, OLEDs fabricated on both flexible IAI stacked anode and conventional ITO/Glass were fabricated and, J-V-L characteristics of those OLEDs were compared by Keithley 2400.

  • PDF

Latex 응집반응을 이용한 동물의 톡소플라즈마병 진단용 kit 개발에 관한 연구 (Development of diagnostic kit(Test-MT) for the microplate latex agglutination test of toxoplasmosis in animal)

  • 서명득;주후돈;데이빗 마-스
    • 대한수의학회지
    • /
    • 제35권3호
    • /
    • pp.583-593
    • /
    • 1995
  • The present study was conducted to develop a toxoplasma latex agglutination test antigen(Test-MT) and evaluate the toxoplasma latex agglutination(LA) test using a newly-made "Test-MT kit" by comparing with the Toxo-MT kit(Eiken chemical co, Tokyo). Also, the specifity and sensitivity test were made by comparing with IFA test and IgG-ELISA. Tachyzoite suspensions of Toxoplasma gondii(RH strain) were ultracentrifuged for 30min at $60,000{\times}g(4^{\circ}C)$ and the supernatant was used as a water-lysate antigen. Polystyrene latex particles of $1.0{\mu}m$ in diameter(Polyscience co) were used for the preparation of sensitized latex-antigen supension(Test-MT). The frequency distribution of LA titers in Test-MT showed two peaks at <1:32 and 1:128. The borderline titer for positive test in Test-MT was determined to be 1:64. But the frequency distribution of LA tites in Toxo-MT showed two peaks at <1:16 and 1:64. The positive borderline was determined to be 1:32. Agreement of reactions between Test-MT and Toxo-MT kit by LA test was shown 92.5% in bovine sera and 97.0% in swine sera, respectively. From the results obtained here it was determined that the sensitized latex-antigen, Test-MT kit, for the microtiter agglutination test prepared as same as by the procedure described in the previous paper(Suh and Lee, 1993) was useful as a highly specific, sensitive and stable immunotiteration reagent for serodiagnosis of toxoplasma infection in animal sera.

  • PDF