• Title/Summary/Keyword: Kim Seung-ok

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Histone deacetylase inhibitor Trichostatin A enhanced the efficiency of adenovirus mediated gene transfer into non-small cell lung cancer cells

  • Park, Mi-Sun;Kang, Ho-Il;Lim, Sin-Ae;Jee, Seung-Wan;Eom, Mi-Ok;Ryeom, Tai-Kyung;Kim, Ok-Hee
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.99.2-99.2
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    • 2003
  • One of the major limitations in using adenoviral vector for gene therapy is inefficient infection of host cells. The presence of coxsackievirus and adenovirus receptor (CAR) and ${\alpha}$-integrin on cell surfaces is required for efficient adenovirus infection. In this study, we investigated the effect of trichostatin A, a histone deacetylase inhibitor, on transfection efficiency after transduction of adenovirus mediated p16$\^$INK4a/ gene transfer. In our previous study, p16$\^$INK4a/ tumor suppressor gene transfer in the non-small cell lung cancer cells (A549 cells) by transduction of recombinant adenovirus (Ad5CMV-p16) resulted in significant inhibition of cancer cell proliferation. (omitted)

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Differential Protein and Gene Expression after Adenovirus-Mediated p16 Gene Transfer in Human Non-Small Cell Lung Cancer Cells

  • Park, Mi-Sun;Kang , Ho-Il;Jee, Seung-Wan;Lim, Si-Nae;Pyo, Jae-Hee;Eom , Mi-Ok;Ryeom , Tai-Kyung;Kim, Ok-Hee
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.291.2-291.2
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    • 2002
  • For the safety evaluation of adenovirus-mediated gene therapy. we have investigated gene and protein expression after transduction of adenoviral vector (Ad5CMV-p16) which contains tumor suppressor gene. p161NK4$\alpha$ in human non-small cell lung cancer (A549) cells. We compared the differential gene expression level in the A549 cells treated with Ad5CMV (null type) and Ad5CMV-p16 virus. respectively. by using cDNA membrane chip and oligonucleotide chip. (omitted)

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Design and Implementation of Personal Internet Broadcasting System Using Host Multicast scheme on FTTH Network Environment (FTTH망에서 호스트멀티캐스트를 이용한 개인 인터넷 방송 시스템 설계 및 구현)

  • Kim Kyoung-Tae;Son Seung-Chul;Heo Kwon;Lee Hyung-Ok;Kim Kyung-Hun;Nam Ji-Seung
    • Proceedings of the Korean Information Science Society Conference
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    • 2006.06d
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    • pp.157-159
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    • 2006
  • 본 논문에서는 FTTH 환경에 의해 가입자까지 100Mbps 이상의 대역폭을 보장하면서 실제 서비스 되어 질 수 있는 응용 시스템인 개인 인터넷 방송 시스템을 호스트 멀티캐스트 Scheme을 이용하여 설계 및 구현하였다. 전송 방식인 호스트 멀티캐스트에 대해 알아보고 개인 인터넷 방송 서버(Contents Provider)와 클라이언트(Host)의 구조에 효율적인 호스트 멀티캐스트 알고리즘인 Data Delivery Tree Algorithm(DDTA)를 제안하고, DDTA 알고리즘이 FTTH 환경에서 HDTV급 고화질 영상을 전송할 수 있는 개인 인터넷 방송 시스템에 효율적인 알고리즘이라는 것을 보인다.

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