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Studies on Residual Effect of Pfosphate Fertilization in Grassland I. Residual effedt of phosphate fertilization on dry matter yield andnutrient productivity of pasture plants in mixed sward (초지에 대한 인산질비료의 잔류효과에 관한 연구 I. 혼파초지에서 목초의 수량 및 양분생산성에 대한 인산질비료의 잔류효과)

  • Park, Geun-Je;Kim, Jeong-Gap;Kim, Meing-Jooung;Seo, Sung
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.16 no.4
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    • pp.260-266
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    • 1996
  • To find out the residual effect of phosphate fertilization level on the dry matter yield and nutrient plloductivity of pasture plants, this experiment was arranged as a randomized complete block design with six treatments(0-0, 50-50, 100-65, 150-65, 200-65 and 250-65kg $P_2O_5$), those were composed of three $P_2O_5$ fertilization level(0,50 and 65kg $P_2O_5$ after phosphate fertilization trial with six treatments(0, 50, 100, 150, 200 and 250kg $P_2O_5$/ha) from 1989 to 1992, and conducted at hilly land in Kwangju, Kyonggi Province from 1993 to 1994. The results obtained are summarized as follows : As the available phosphate of soil increase, the early growth and cover degree of pasture plants wintered were more favorable. With 150-65kg $P_2O_5$ application for two years, the average dry matter yield of grasses, 9,862kglha was similared to that of 200-65kg $P_2O_5$/ha. The crude protein and energy productivity of pasture plants tended to increase as available phosphate of soil was increased, but those were not different between 150-65 and 250-65kg P 2 0 4 a Efficiency of $P_2O_5$ was highest at $P_2O_5$ 150-65kg/ha fertilization, wHich produced dry matter 11 1.8kg. net energy lactation(NEL) 67 1.2M.f per I kg P205. Therefore, phosphate fertilization is recommended strongly application with 150kg/ha for three years after pasture establishment, and then 65kg/ha for two years a reasonabl management in hilly grassland.

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Induced Ovulation in the Mandarin Fish, Siniperca scherzeri by Sex-Maturation Hormones (성성숙 호르몬 처리에 의한 쏘가리의 배란 유도)

  • 장선일;이완옥;이종윤;손송정
    • Journal of Aquaculture
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    • v.11 no.4
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    • pp.513-519
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    • 1998
  • Ovulation of maturing femal mandarin fish, Siniperca scherzeri was induced using single injection of human chorionic gonadotropin (HCG) or gonadotropin releasing hormone-analogue (GnRH-a), GnRH-a plus prostaglandin F2 (PG$F_2$) or GnRH-a plus pimozide. The response was evaluated by fertilization, embryo-formation and hatching rate after insemination. Those rates were generally higher in GnRH-a group than in HCG group. The higher hatching rat of above 89% was achived using a dosage of 5,000 IU/kg HCG plus 10 ${\mu}$g/kg GnRH-a, 10${\mu}$g/kg GnRH-a plus 500 ng/kg PGF2, and 10 ug/kg GnRH-a plus 1-5 mg/kg pimozide. Ovulation was induced in all female injected with sex-maturation hormones and stimulator, but blocked in female injected with HCG plus GnRH-a plus dopamine combination, and GnRH-a plus PGF2 plus indometacin combination. These results show that the mandarin fish in spawning period secrete a sex-mutruation assosiated hormones and gonadotropin-releasing -inhibiting factor(GRIF).

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Effect of Seedling and Division Nursery Stock Size on Growth and Yield in Water Culture Condition of Wasabia japonica Matsum (고추냉이 물재배시 묘크기가 생육 및 수량에 미치는 영향)

  • 변학수
    • Korean Journal of Plant Resources
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    • v.10 no.4
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    • pp.375-381
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    • 1997
  • This study was conducted to determine the effect of seedling and division nursery stock size on the growth and yield of Wasabia japonica cultivated with cold water from Soyang Dam. As seedling and division nursery stock size were enlarged, their growth and yield were higher. In the case of division nursery stock, the rhizome yield per 10a were 782kg for large one, 723kg form medium one and 567kg for small one, and commercial rates - the percentage of rhizomes that weigh over 40g - were 87% for large one,80% for medium one and 55% for small one. In the case of seedling, large one brought out yield of 575kg/10a while medium and small one brought out low yield of 408kg/10a, and 421kg/10a, respectively. And the commercial rates from seedling were 60% for large one. 13% for medium one and 20% for small one.

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Textural Changes of Cooked-Rice during Warm Keeping in Electrical Rice Cooker (전기밥솥에서 보온중인 밥의 조직감 변화)

  • Lee, Y.J.;Hwang, S.W.;Kim, S.K.;Park, Y.S.;Chun, J.K.
    • Applied Biological Chemistry
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    • v.38 no.5
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    • pp.422-424
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    • 1995
  • In order to investigate the textural changes of cooked rice in a home electrical rice cooker during warm keeping period, rice was cooked and stored at $73^{\circ}C$ for 42 hours. The time course changes of hardness and adhesiveness of cooked rice were measured with the specimen prepared as a cylindrical rice block(H/D= 15 mm/20 mm). At the initial stage of warm keeping period the hardness was 1.61 kg and it was increased steadily to 1.95 kg up to 12 hours. And it was rapidly dropped to 1.57 kg within 3 hours, and thereafter gradually decreased to 1.44 kg. The adhesiveness was steadily declined with warm keeping period from 0.51 to 0.323kg set The cohesiveness and chewiness were also changed in the similar pattern to that of hardness while the springiness remained unchanged throughout the whole warm keeping period.

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Residues of Heavy Metals and Organochlorine Pesticides in Ginseng at Environment of North Gyeongbuk (경북북부 인삼 재배환경 중 중금속과 유기염소계 농약의 잔류)

  • Park, Soo-Woon;Kim, Jung-Ho
    • Journal of Environmental Science International
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    • v.19 no.10
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    • pp.1203-1210
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    • 2010
  • To obtain the risk assessment of hazardous materials in ginseng, the residues of heavy metals and organochlorine pesticides in samples on the Yeungju and Sangju are surveyed. Cd and Hg in ginseng on Yeungju and Sangju is not detected. 0.21 mg/kg of As, 0.39 mg/kg of Cr, 0.14 mg/kg of Pb, 1.83 mg/kg of Cu, 3.93 mg/kg of Zn and 0.43 mg/kg of Ni in ginseng on Sangju are shown. 0.25 mg/kg of Cr, 0.08 mg/kg of Pb, 1.11 mg/kg of Cu, 8.99 mg/kg of Zn and 1.15 mg/kg of Ni in ginseng on Yeungju are detected. As and Pb in ginseng on Sangju are 7.0% and 2.8% for Korea Food & Drug Administration(KFDA) advisory level, respectively. Pb in ginseng on Yeongju are 1.6% for Korea Food & Drug Administration(KFDA) advisory level. The heavy metals such as AS, Cr, Cd, Pb, Cu, Zn, Hg, Ni in soil on Yeongju and Sangju are range of 0-20% for with Korea Food & Drug Administration (KFDA) level. BHC isomer, DDT isomer and endosulfan isomer are not detected in ginseng and soil on Yeongju and Sangju, respectively. Finally, the assessment of hazardous materials of the heavy metals and organochlorine pesticides in ginseng on the Youngju and Sangju are verified the safety the level compare with tolerances level for Korea.

Nutrient Turnover by Fine Roots in Temperate Hardwood and Softwood Forest Ecosystems Varying in Calcium Availability

  • Park, Byung Bae
    • Journal of Korean Society of Forest Science
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    • v.96 no.2
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    • pp.214-221
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    • 2007
  • The effect of nutrient availability and forest type on the nutrient turnover of fine roots is important in terrestrial nutrient cycling, but it is poorly understood. I measured nutrient turnover of hardwoods and softwoods at three well studied sites in the northeastern US: Sleepers River, VT; Hubbard Brook, NH; Cone Pond, NH. Significant differences in nutrient turnover by fine roots were observed among sites, but not between forest types. The magnitude of differences for each element ranged from 3 times for P and N to 8 times for Ca and Mg between sites. Smaller differences of 0.2 to 0.8 times were observed between forest types. In hardwoods, the Sleepers River 'new' site had $23kg\;ha^{-1}\;yr^{-1}$ Ca, $7kg\;ha^{-1}\;yr^{-1}$ Mg, and $16kg\;ha^{-1}\;yr^{-1}$ K turnover, owing to high root nutrient contents and turnover. Cone Pond had the highest turnover for Mn ($0.8kg\;ha^{-1}\;yr^{-1}$) and Al ($16kg\;ha^{-1}\;yr^{-1}$), owing to high nutrient contents. The Hubbard Brook hardwood site exhibited the lowest turnover of these elements. In softwoods, the variation in turnover of Ca, Mg, and K was lower than in hardwoods. The Hubbard Brook had the highest turnover for P ($1.6kg\;ha^{-1}\;yr^{-1}$), N ($31kg\;ha^{-1}\;yr^{-1}$), Mn ($0.4kg\;ha^{-1}\;yr^{-1}$), Al ($10kg\;ha^{-1}\;yr^{-1}$), Fe ($6.4kg\;ha^{-1}\;yr^{-1}$), Zn ($0.3kg\;ha^{-1}\;yr^{-1}$), Cu ($34g\;ha^{-1}\;yr^{-1}$), and C ($1.1Mg\;ha^{-1}\;yr^{-1}$). Root Ca turnover exponentially increased as soil percentage Ca saturation increased because of greater root nutrient contents and more rapid turnover at the higher Ca sites. These results imply that nutrient inputs by root turnover significantly increase as soil Ca availability improves in temperate forest ecosystems.

Heavy metal assessment of marine sediment in selected coastal districts of the Western Region, Ghana

  • Kuranchie-Mensah, Harriet;Osei, Juliet;Atiemo, Sampson M.;Nyarko, Benjamin J.B.;Osae, Shiloh K.;Laar, Cynthia;Ackah, Michael;Buah-Kwofie, Archibold;Blankson-Arthur, Sara;Adeti, Prince J.
    • Advances in environmental research
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    • v.2 no.2
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    • pp.155-166
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    • 2013
  • A preliminary investigation to establish the status of contamination of trace metals in the Western coast of Ghana was conducted prior to the commercial production of crude oil in the area. The study revealed the presence of heavy metals such as Pb (4.00-79.64 mg/kg), As (8.81-236 mg/kg), Cu (12.86-108.06 mg/kg), V (28.07-953.32 mg/kg), Zn (7.08-264.25 mg/kg), Cr (101.69-1366.62 mg/kg), Ni (42.41-451.43 mg/kg), Mn (16.77-1890.45mg/kg), Br (7.66-142.78 mg/kg), Ti (542.03-19960 mg/kg) and Fe (7472.88-97120 mg/kg) at six sites sampled along the coast. With the exception of Ti and Fe which showed no variation in metal concentration, the rest of the metals varied significantly among the sampled locations. Potential ecological risk of metals particularly of Co, As and Br which exhibited extreme enrichment of the sediments indicates considerable metal pollution in the studied areas. The degree of contamination is of particular concern especially to benthic biota that inhabit this environment for survival.

Biochemical and Histopathological Changes of Rockfish, Sebastes schlegeli by Dietary Benzo(a)pyrene (사료를 통한 Benzo(a)pyrene 노출에 따른 조피볼락, Sebastes schlegeli의 생화학 및 조직병리학적 변화)

  • Park Dae Kuk;Kim Jae Won;Jee Jung-Hoon;Park Soo Il;Kang Ju Chan
    • Korean Journal of Environmental Biology
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    • v.22 no.3
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    • pp.387-393
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    • 2004
  • Experiments were carried out to investigate the lipid peroxidation (LPO), superoxide dismutase (SOD) activity and histopathological change of hepatic tissue for rockfish, Sebastes schlegeli after feeding sub-chronic dietary Benzo(a)pyrene (BaP) in the concentration of 0 (control), 0.5, 1.0, 1.5, 2.0 mg $kg^{-1}$ dry food of diet for 30 days. In 2.0 mg $kg^{-1}$ dry food group, the significant increase of LPO was observed in all period, and SOD activity was incresed at 30 days significantly in the same concentration. In the histological investigation of liver, there was the swelling of hepatic cells at 10 days over the 1.0 mg $kg^{-1}$ dry food concentration. At 30 days Periodic acid-Schiff (PAS) positive granule was observed in the same group and at 20 days was observed in 2.0 mg $kg^{-1}$ dry food group. And there was necrosis of hepatic cell in some fish of 2.0 mg $kg^{-1}$ dry food group at 30 days.

Effects of Pig Manure and Wine Sludge on Soil Nitrification in Greenhouse (돈분발효퇴비와 포도주부산물이 시설 내 토양의 질산화에 미치는 영향)

  • Shin, Myung-Sook;Jeon, Hee
    • Proceedings of the Korean Society for Bio-Environment Control Conference
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    • 2001.04b
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    • pp.67-68
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    • 2001
  • 돈분발효퇴와 포도주 부산물의 질소 무기화 및 질산화 과정을 구명하기 위해 시설 내 고추생육시기에 토층별 암모늄태 및 질산태 질소함량을 조사하였다. 질소무기화 및 질산화는 심토층보다 표토층에서 높게 나타났다. 생육전반에 걸쳐 총 질소 무기화 및 질산화율은 처리간에 다양하였지만, 전반적으로 총 질소 무기화는 정식후 90일 까지 증가하였지만 그 이후로는 감소하였다. 표토층에 있어 최고 질소 무기화 및 질산화는 정식후 90일에 T4(포도주부산물 400kg/10a+돈분발효퇴비 1,000kg/10a)에서 관측되었다. 가장 높은 질소 무기화는 정식후 30일에 대조구에서 272.5mg/kg과 정식 후 90일에 T4(포도주부산물 400kg/10a+돈분발효퇴비 1,000kg/10a)에서 843.4mg/kg으로 나타났다. 또한 질산화는 T4(포도주부산물 400kg/10a+돈분발효퇴비 1,000kg/10a)에서 정식 후 90일에 872.2mg/kg으로 가장 높게 나타났다. 15-30cm 토층의 질산화 현상은 질소 무기화와는 다르게 나타났는데 질소 무기화는 대체로 90일에 증가한 반면 질산화는 90일에 감소하는 경향을 나타내었다. 마지막 조사시기인 정식 후 180일에는 급격히 감소하는 현상을 보였는데 고추 생육에 따라 양분 흡수로 암모늄태질소 및 질산태질소가 감소되었다. 0-15cm 토층, 15-30cm 토층의 질소무기화 및 질산화에서 T4(포도주부산물 400kg/10a+돈분발효퇴비 1,000kg/10a)의 15-30cm 토층 질산화가 낮게 나타났다.

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Effects of the Pharmacopuncture with MOK on Immune Regulation by Th1/Th2 Cytokines in L-Thyroxine-Induced Hyperthyroid Rats (L-thyroxine으로 유발된 갑상선기능항진증 랫트에서 MOK 약침의 Th1/Th2 사이토카인 조절에 의한 면역조절 효과)

  • Hwang, Ji Hye;Jung, Hyo Won
    • Korean Journal of Acupuncture
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    • v.35 no.2
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    • pp.91-97
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    • 2018
  • Objectives : This study was performed to investigate immune regulatory effects of the pharmacopuncture with MOK on hyperthyroid rats. Methods : The experimental hyperthyroidism was prepared by the intraperitoneal injection of L-thyroxine(LT4, 0.5 mg/kg) once daily for 2 weeks in Sprague-Dawley(SD) rats. The pharmacopuncture with MOK extract(MOK pharmacopuncture) at doses of 0.3 or 3 mg/kg was injected on acupuncture points in the thyroid glands of hyperthyroid rats once a day for 2 weeks. Propylthiouracil(PTU, 10 mg/kg) as a reference group was subcutaneously injected into the dorsal neck. We measured the levels of $IFN-{\gamma}$ and IL-4 in the sera of rats using enzyme-linked immunosorbant assay(ELISA) and determined the expression of $IFN-{\gamma}$, IL-4, IL-10, and Foxp3 in spleen tissues by reverse transcriptase-polymerase chain reaction(RT-PCR). Results : The treatment of MOK pharmacopuncture in hyperthyroid rats significantly decreased the serum levels of Th1 cytokine, $IFN-{\gamma}$(p<0.01 for MOK 0.3 mg/kg, p<0.05 for MOK 3 mg/kg, and p<0.05 for PTU) and significantly increased the levels of Th2 cytokine, IL-4(p<0.05 for MOK 0.3 mg/kg, p<0.001 for MOK 3 mg/kg, and p<0.05 for PTU) compared to control group. Also, the MOK pharmacopuncture significantly increased IL-4 expression(p<0.05 for MOK 3 mg/kg, and p<0.05 for PTU), IL-10(p<0.05 for MOK 3 mg/kg, and p<0.01 for PTU), and Foxp3(p<0.01 for MOK 0.3 mg/kg, p<0.05 for MOK 3 mg/kg and p<0.01 for PTU) in spleen tissues of hyperthyroid rats compared to control group. Conclusions : Our results suggest that MOK pharmacopuncture can help to ameliorate the pathological progression of hyperthyroidism by regulation of the Th1/Th2 imbalance.