• International Journal of Advanced Culture Technology
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• 제6권4호
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• pp.284-291
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• 2018

As environmental pollution has become worse green technologies to replace or reduce consumption of fossil fuel get spotlight from government, industry and academia globally. It is reported that 40% of carbon dioxide emission is caused by electricity power generation. And 37% of end user electricity power is used by residential costumer in US. Smart Grid is considered as one of promising technology to alleviate severe environmental problem. In residential environment, Home Energy Management System (HEMS) can play a key role for green smart home. The HEMS can give several benefits like aslowering electric utility bill, improvement of efficiency of electric power consumption and integration of generator using renewable energy resources. However just limited functions of HEMS can be used for residential customer in real life because of lack of smart function in home appliances and optimal managing software for HEMS. This study provides comprehensive analysis for Home Energy Management System for residential customer. Simple HEMS system with real products on the market are explained and limitation of current HEMS are also discussed.

• Journal of Microbiology and Biotechnology
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• 제23권2호
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• pp.246-250
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• 2013

In this study, a loop-mediated isothermal amplification (LAMP) method to rapidly detect Staphylococcus aureus strains was developed and evaluated by extensively applying a large number of S. aureus isolates from clinical and food samples. Six primers were specially designed for recognizing eight distinct sequences on the species-specific femA gene of S. aureus. The detection limits were 100 fg DNA/tube and $10^4$ CFU/ml. The LAMP assay was applied to 432 S. aureus strains isolated from 118 clinical and 314 food samples. Total detection rates for the LAMP and polymerase chain reaction assays were 98.4% (306/311) and 89.4% (278/311), respectively.

• Journal of Contemporary Eastern Asia
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• 제13권2호
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• pp.41-56
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• 2014

This research paper advances our understanding of complex interdependence among countries. Existing research has found that total factor productivity (TFP), the residual from the economic growth function, is hindered in the absence of a country's strong political and legal institutions or if a country does not already have a sufficiently high level of TFP. We also know that regional efforts to eliminate pollution are complex. Bridging these two areas while focusing on a high polluting yet high innovating region, the following research questions are posed: Are Northeast Asian countries key collaborators in pursuit of green R&D? Are Northeast Asian countries collaborating extensively with each other? What are the implications for other regions' attempts to establish these kinds of relations? To answer the above questions, biofuels-related technology as defined in the International Patent Classification's "green inventory" of environmentally sound technologies is examined. Patent data is drawn from the USPTO and inventors' country origin as the unit of analysis. For the 1990-2013 period, the Northeast Asian countries are in the core of a small set of collaborating countries. There is evidence that their centrality has increased in recent years. Most importantly, East Asia is becoming a singular research hub in terms of biofuels-related R&D, offering a counter in the foreseeable future to the dominance of the American and European research network hubs.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.150.1-150
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• 2003

A single-step multiplex reverse transcription polymerase chain reaction (RT-PCR) assay was developed for the simultaneous detection of five cucurbit-infecting viruses： cucumber mosaic virus (CMV), watermelon mosaic virus 2 (WMV2), zucchini yellow mosaic virus (ZYMV), cucumber green mottle mosaic virus (CGMMV), and kyuri green mottle mosaic virus (KGMMV). The multiplex RT-PCR provides a simple and rapid method for detecting various viruses in cucurbit plants, which will help diagnose many cucurbit plants at a time.

• Industrial Engineering and Management Systems
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• 제12권2호
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• pp.161-170
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• 2013

Deployment of green transportation in reverse logistics is a key issue for low carbon technologies. To cope with such logistic innovation, this paper proposes a hybrid approach to solve practical vehicle routing problem (VRP) of pickup type that is common when considering the reverse logistics. Noticing that transportation cost depends not only on distance traveled but also on weight loaded, we propose a hierarchical procedure that can design an economically efficient reverse logistics network even when the scale of the problem becomes very large. Since environmental concerns are of growing importance in the reverse logistics field, we need to reveal some prospects that can reduce $CO_2$ emissions from the economically optimized VRP in the same framework. In order to cope with manifold circumstances, the above idea has been deployed by extending the Weber model to the generalized Weber model and to the case with an intermediate destination. Numerical experiments are carried out to validate the effectiveness of the proposed approach and to explore the prospects for future green reverse logistics.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.159-159
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• 2017

Drought, a common environmental constraint, induces a range of physiological, biochemical and molecular changes in plants, and can cause severe reductions in crop yield. Consequently, understanding the molecular mechanisms of drought tolerance is an important step towards crop biotechnology. Here, we report that the rice (Oryza sativa) homeodomain-leucine zipper class IV transcription factor gene, ${\underline{R}ice}$ ${\underline{o}utermost}$ ${\underline{c}ell-specific}$ gene 10 (Roc10), enhances drought tolerance and grain yield by increasing lignin accumulation in ground tissues. Overexpression of Roc10 in rice significantly increased drought tolerance at the vegetative stages of growth and promoted both more effective photosynthesis and a reduction in water loss rate, compared with non-transgenic controls or RNAi transgenic plants. Importantly, Roc10 overexpressing plants had a higher drought tolerance at the reproductive stage of growth and a higher grain yield compared with the controls under field-drought conditions. Roc10 is mainly expressed in outer cell layers including the epidermis and the vasculature of the shoots, which coincides with areas of cell wall lignification. Roc10 overexpression elevated the expression levels of lignin biosynthetic genes in shoots, with a concomitant increase in the accumulation of lignin, while the overexpression and RNAi lines showed opposite patterns of lignin accumulation. We identified downstream target genes of Roc10 by performing RNA-seq and chromatin immunoprecipitation (ChIP)-seq analyses of shoot tissues. Roc10 was found to directly bind to the promoter of PEROXIDASEN/PEROXIDASE38, a key gene in lignin biosynthesis. Together, our findings suggest that Roc10 confers drought stress tolerance by promoting lignin biosynthesis in ground tissues.

• 한국환경복원기술학회지
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• 제24권3호
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• pp.1-18
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• 2021

England's green belt policy as it delivered its framework to designating the development restriction zones(DRZ) in Korea is the key to address issues which try to change green belt boundary or/and housing development. Therefore it should necessarily be reviewed on the understanding of how England has been formulating a national policy to deal with the issues focusing on opportunities for housing development and recreation. This study explored the discourse and framework of England's green belt policy as well as driver changes of housing development and recreation. Results show several characteristics of England's green belt policy which are civil society consensus on conservation and management, limited small-scale housing development through management of release rates, a systematic procedure for application and approval, open-recreation space expansion and utilisation under the premise of conservation of natural green areas, and management structure by the involvement of NGO organisations. Therefore, five suggestions can be delivered to developing Korea's DRZ frameworks: first, preceding social consensus on the preservation value of development-restricted zones, second, addressing housing shortages in different alternatives e.g.) environmental-friendly small-scale housing, third, institutionalising the total proportion of release, fourth, establishing an open-recreation space, fifth, introducing expanded public-private partnerships. Ultimately securing the legitimacy of the nation's development-restricted zone system can contribute positively to the environment preservation and human health by promoting public leisure activities in terms of the recent increase in external activities caused by the Covid-19 crisis. Concluding remarks are here that the understanding of England's green belt policy can be delivered to and help formulate domestic policy addressing current issues.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제15권7호
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• pp.2496-2512
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• 2021

The requirements for powerful computing capability, high capacity, low latency and low energy consumption of emerging services, pose severe challenges to the fifth-generation (5G) network. As a promising paradigm, mobile edge networks can provide services in proximity to users by deploying computing components and cache at the edge, which can effectively decrease service delay. However, the coexistence of heterogeneous services and the sharing of limited resources lead to the competition between various services for multiple resources. This paper considers two typical heterogeneous services: computing services and content delivery services, in order to properly configure resources, it is crucial to develop an effective offloading and caching strategies. Considering the high energy consumption of 5G base stations, this paper considers the hybrid energy supply model of traditional power grid and green energy. Therefore, it is necessary to design a reasonable association mechanism which can allocate more service load to base stations rich in green energy to improve the utilization of green energy. This paper formed the joint optimization problem of computing offloading, caching and resource allocation for heterogeneous services with the objective of minimizing the on-grid power consumption under the constraints of limited resources and QoS guarantee. Since the joint optimization problem is a mixed integer nonlinear programming problem that is impossible to solve, this paper uses deep reinforcement learning method to learn the optimal strategy through a lot of training. Extensive simulation experiments show that compared with other schemes, the proposed scheme can allocate resources to heterogeneous service according to the green energy distribution which can effectively reduce the traditional energy consumption.

• Journal of Microbiology and Biotechnology
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• 제24권2호
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• pp.152-159
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• 2014

The regulation of protein tyrosine phosphorylation is mediated by protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs) and is essential for cellular homeostasis. Co-expression of PTKs with PTPs in Pichia pastoris was used to facilitate the expression of active PTKs by neutralizing their apparent toxicity to cells. In this study, the gene encoding phosphatase PTP1B with or without a blue fluorescent protein or peroxisomal targeting signal 1 was cloned into the expression vector pAG32 to produce four vectors. These vectors were subsequently transformed into P. pastoris GS115. The tyrosine kinases EGFR-2 and $PDGFR{\beta}$ were expressed from vector pPIC3.5K and were fused with a His-tag and green fluorescent protein at the N-terminus. The two plasmids were transformed into P. pastoris with or without PTP1B, resulting in 10 strains. The EGFR-2 and $PDGFR{\beta}$ fusion proteins were purified by $Ni^{2+}$ affinity chromatography. In the recombinant P. pastoris, the PTKs co-expressed with PTP1B exhibited higher kinase catalytic activity than did those expressing the PTKs alone. The highest activities were achieved by targeting the PTKs and PTP1B into peroxisomes. Therefore, the EGFR-2 and $PDGFR{\beta}$ fusion proteins expressed in P. pastoris may be attractive drug screening targets for anticancer therapeutics.

• Journal of Microbiology and Biotechnology
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• 제25권7호
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• pp.988-998
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• 2015

The filamentous fungus Aspergillus oryzae is a well-known expression host used to express homologous and heterologous proteins in a number of industrial applications. To facilitate higher yields of proteins of interest, we constructed the pAsOP vector to express heterologous proteins in A. oryzae. pAsOP carries a selectable marker, pyrG, derived from Aspergillus nidulans, and a strong promoter and a terminator of the amyB gene derived from A. oryzae. pAsOP transformed A. oryzae efficiently via the PEG-CaCl₂-mediated transformation method. As proof of concept, green fluorescent protein (GFP) was successfully expressed in A. oryzae transformed by pAsOP-GFP. Additionally, we identified a novel fungal α-amylase (PcAmy) gene from Penicillium sp. and cloned the gene into the vector. After transformation by pAsOPPcAmy, the α-amylase PcAmy from Penicillium sp. was successfully expressed in a heterologous host system for the first time. The α-amylase activity in the A. oryzae transformant was increased by 62.3% compared with the untransformed A. oryzae control. The PcAmy protein produced in the system had an optimum pH of 5.0 and optimum temperature of 30^oC. As a cold-adapted enzyme, PcAmy shows potential value in industrial applications because of its high catalytic activity at low temperature. Furthermore, the expression vector reported in this study provides promising utility for further scientific research and biotechnological applications.