• Title/Summary/Keyword: Key Gas Method

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Breeding of Early Heading Date with High Yield Using CRISPR/Cas9 in Rice

  • Eun-Gyeong Kim;Jae-Ryoung Park;Yoon-Hee Jang;Kyung-Min Kim
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.285-285
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    • 2022
  • Recent unpredictable climate change is a major cause of rice yield loss. In particular, methane is a key factor in global warming. Therefore rice breeders are trying to breed the reducing-methane gas emission rice using the crossbreeding method. However, the traditional crossbreeding method takes 8 to 10 years to breed a cultivar, and the anther culture method developed to shorten the breeding cycle also takes 6 to 7 years. On the other hand, CRISPR/Cas9 accurately edits the target trait and can rapidly breed rice cultivars by editing the target trait as a homozygous in 2-3 years. In addition, exogenous genetic elements such as Cas9 can be isolated from the G1 generation. Therefore, the flowering time was regulated by applying CRISPR/Cas9 technology, and OsCKq1 genome-editing (OsCKq1-G) rice with early flowered and high yield was bred in the field. Genome-editing of OsCKq1 applied CRISPR/Cas9 technology up-regulates the expression of the flowering promotion gene Ehd1 under long-day conditions induces early flowering and increases the yield by increasing the 1,000-grain weight. And as the generations advanced, each agricultural trait indicated a low coefficient of variation. As a result, indicated that OsCKq1 plays an important role in regulating the flowering time and is related to the trait determining yield. Therefore, OsCKq1-G can suggest a breeding strategy for the Net-Zero national policy for reducing-methane gas emission rice by shortening the breeding cycle with the early flowered, and high-yield rice. CRISPR/Cas9 technology is a rapid and accurate breeding technology for breeding rice cultivars with important characteristics.

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Seismic interval velocity analysis on prestack depth domain for detecting the bottom simulating reflector of gas-hydrate (가스 하이드레이트 부존층의 하부 경계면을 규명하기 위한 심도영역 탄성파 구간속도 분석)

  • Ko Seung-Won;Chung Bu-Heung
    • 한국신재생에너지학회:학술대회논문집
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    • 2005.06a
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    • pp.638-642
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    • 2005
  • For gas hydrate exploration, long offset multichannel seismic data acquired using by the 4km streamer length in Ulleung basin of the East Sea. The dataset was processed to define the BSRs (Bottom Simulating Reflectors) and to estimate the amount of gas hydrates. Confirmation of the presence of Bottom Simulating reflectors (BSR) and investigation of its physical properties from seismic section are important for gas hydrate detection. Specially, faster interval velocity overlying slower interval velocity indicates the likely presences of gas hydrate above BSR and free gas underneath BSR. In consequence, estimation of correct interval velocities and analysis of their spatial variations are critical processes for gas hydrate detection using seismic reflection data. Using Dix's equation, Root Mean Square (RMS) velocities can be converted into interval velocities. However, it is not a proper way to investigate interval velocities above and below BSR considering the fact that RMS velocities have poor resolution and correctness and the assumption that interval velocities increase along the depth. Therefore, we incorporated Migration Velocity Analysis (MVA) software produced by Landmark CO. to estimate correct interval velocities in detail. MVA is a process to yield velocities of sediments between layers using Common Mid Point (CMP) gathered seismic data. The CMP gathered data for MVA should be produced after basic processing steps to enhance the signal to noise ratio of the first reflections. Prestack depth migrated section is produced using interval velocities and interval velocities are key parameters governing qualities of prestack depth migration section. Correctness of interval velocities can be examined by the presence of Residual Move Out (RMO) on CMP gathered data. If there is no RMO, peaks of primary reflection events are flat in horizontal direction for all offsets of Common Reflection Point (CRP) gathers and it proves that prestack depth migration is done with correct velocity field. Used method in this study, Tomographic inversion needs two initial input data. One is the dataset obtained from the results of preprocessing by removing multiples and noise and stacked partially. The other is the depth domain velocity model build by smoothing and editing the interval velocity converted from RMS velocity. After the three times iteration of tomography inversion, Optimum interval velocity field can be fixed. The conclusion of this study as follow, the final Interval velocity around the BSR decreased to 1400 m/s from 2500 m/s abruptly. BSR is showed about 200m depth under the seabottom

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Measurement of Bubble Size in Flotation Column using Image Analysis System (이미지 분석시스템을 이용한 부선컬럼에서 기포크기의 측정)

  • An, Ki-Seon;Jeon, Ho-Seok;Park, Chul-Hyun
    • Resources Recycling
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    • v.29 no.6
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    • pp.104-113
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    • 2020
  • Bubble size in froth flotation has long been recognized as a key factor which affects the bubble residence time, the bubble surface area flux (Sb) and the carrying rate (Cr). This paper presents method of bubble size measurement, relationship between operating variables and gas dispersion properties in flotation column. Using high speed camera and image analysis system, bubble size has been directly measured as a function of operating parameters (e.g., superficial gas rate (Jg), superficial wash water rate (Jw), frother concentration) in flotation column. Relationship compared to measured and estimated bubble size was obtained within error ranges of ±15~20% and mean bubble size was 0.718mm. From this system the empirical relationship to control the bubble size and distribution has been developed under operating conditions such as Jg of 0.65~1.3cm/s, Jw of 0.13~0.52cm/s and frother concentration of 60~200ppm. Surface tension and bubble size decreased as frother concentration increased. It seemed that critical coalescence concentration (CCC) of bubbles was 200ppm so that surface tension was the lowest (49.24mN/m) at frother concentration of 200ppm. Bubble size tend to increase when superficial gas rate (Jg) decreases and superficial wash water rate Jw and frother concentration increase. Gas holdup is proportional to superficial gas rate as well as frother concentration and superficial wash water rate (at the fixed superficial gas rate).

Assessing the Geometric Integrity of Cylindrical Storage Tanks: A Comparative Study Using Static Terrestrial Laser Scanning and Total Station

  • Mansour Alghamdi;Jinha Jung
    • Korean Journal of Remote Sensing
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    • v.40 no.3
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    • pp.243-255
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    • 2024
  • This study compares Static Terrestrial Laser Scanning (STLS)with the conventional Total Station (TS) method for the geometric assessment of cylindrical storage tanks. With the crucial need for maintaining tank integrity in the oil and gas industry, STLS and TS methods are evaluated for their efficacy in assessing tank deformations. Using STLS and TS, the roundness and verticality of two cylindrical tanks were examined. A deformation analysis based on American Petroleum Institute (API) standards was then provided. Key objectives included comparing the two methods according to API standards, evaluating the workflow for STLS point cloud processing, and presenting the pros and cons of the STLS method for tank geometric assessment. The study found that STLS, with its detailed and high-resolution data acquisition, offers a substantial advantage in having a comprehensive structural assessment over TS. However, STLS requires more processing time and prior knowledge about the data to tune certain parameters and achieve accurate assessment. The project outcomes intend to enhance industry professionals' understanding of applying STLS and TS to tank assessments, helping them choose the best method for their specific requirements.

Evaluation of Thermal Fluid Characteristics for EGR Cooler with Spiral Type (Spiral 구조 EGR Cooler의 열유동 특성 평가)

  • 허형석;원종필;박경석
    • Transactions of the Korean Society of Automotive Engineers
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    • v.11 no.6
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    • pp.44-50
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    • 2003
  • Cooled EGR is an effective method for the reduction of NOx from a diesel engine and an EGR Cooler is the key component of the system. High efficiency, low pressure loss and compactness are required for the EGR Cooler. To meet these requirements, new geometric tube must be developed. In this paper, a full size EGR cooler test bench has been developed to validate the CFD flow and heat transfer models. Fluid temperature and pressure drop measurements are provided. fillet temperature is $200^{\circ}C$ and $300^{\circ}C$, and flow rates vary from 0.008 kg/sec to 0.019 kg/sec. The gas flow and heat transfer in a single tube cooler have been studied using computational fluid dynamics(CFD). Analysis has been carried out in a single tube with a plain tube and six spirally enhanced tubes of varying pitch to depth ratio(p/e).

Comparison of GC-Profile on Tobacco Smoke Components (담배 연기성분의 GC-Profile 특성비교)

  • 나효환;한상빈;복진영;이운철;백순옥;장기철;양광규
    • Journal of the Korean Society of Tobacco Science
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    • v.16 no.2
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    • pp.152-162
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    • 1994
  • This study was designed to establish an analytical method for the properties of leaf tobacco smoke. Lyophilized TPM from leaf tobacco smoke was extracted with MeOH, dried under reduced pressure, and trimethyl-silylated(TMS). Gas chromatography of the material using SPB-5 column showed 120 quantifiable peaks. Among those, 26 compounds including a hydrocarbons, Neophytadiene. and Levulinic acid could be identified through GC-MS. Smoke properties of 5 manufacturing grades and 2 oriental cultivars of domestic and imported leaf tobacco including AB3O-1 were analyzed. For flue-cured tobacco, content of the compounds in the smoke was generally higher in American leaf tobacco except for glycerol compounds. For burley tobacco, domestic leaves were found to have much higher amount of smoke compound than imported leaves. Among oriental tobacco, Izmir contained slightly higher amount of smoke compounds than Basma. Key words : GC - profile. TPM. TMS. Leaf tobacco.

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Mixing Effect by the Geometry of Static Mixer with Turbulent In-Situ Mixing Process (난류 용탕 In-Situ 합성법을 위한 스태틱 믹서의 형상에 따른 혼합 효과)

  • Lee, Dae-Sung;Kim, Hyo-Geun;Ha, Man-Yeong;Park, Yong-Ho;Park, Ik-Min
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.29 no.12 s.243
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    • pp.1307-1312
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    • 2005
  • Turbulent in-situ mixing process is a new material process technology to get dispersed phase in nanometer size by controlling reaction of liquid/liquid, liquid/solid and liquid/gas, flow and solidification speed simultaneously. In this study mixing, the key technology to this synthesis method will be studied by computational fluid dynamics. For the simulation of mixing of liquid metal, static mixers will be investigated. Two inlets for different liquid metal meet and merge like 'Y' shape tube. The tube has various shapes such as straight and curved. Also, the radius of curve will be varied. The performance of mixer will be evaluated with quantitative analysis with coefficient of variance of mass fraction. Also, detailed plots of intersection will be presented to understand effect of mixer shape on mixing.

Combustion Stability Analysis using Feedback Transfer Function (피드백 전달함수를 이용한 연소 안정성 해석)

  • Kim, Jina;Yoon, Myunggon;Kim, Daesik
    • Journal of the Korean Society of Combustion
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    • v.21 no.3
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    • pp.24-31
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    • 2016
  • In this paper we propose a new approach for an analysis and a prediction of combustion instability of lean premixed gas turbines. Our approach is based on the Nyquist stability criterion in control theory and a transfer function representation of a one-dimensional (1D) thermoacoustic system. A key advantage of the proposed approach is that one can systematically characterize the effects of various parameters of a combustor system on combustion instability. Our analysis method was applied to a real combustion system and the analysis results were consistent with experimental data.

Dual Address Electrodes for Fast Addressing Method of ac-PDP with High Xe% Working Gas

  • Lee, D.K.;Choi, J.H.;Choi, W.S.;Ok, J.W.;Kwon, B.S.;Lee, H.J.;Lee, H.J.;Kim, D.H.;Park, C.H.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2005.07a
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    • pp.247-250
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    • 2005
  • In this paper, new address electrode having separated dual electrodes is suggested to reduce addressing time in ac PDP. It had been found that both the formative and jitter width of the suggested electrode are improved by $10{\sim}20$ % compared with the conventional one on IMID 04'. So we experiment other several kinds of the separated electrodes, and the change in discharge characteristics is analyzed by using a two-dimensional fluid simulation. The key feature of the suggested structure is that the distribution of Xe and Ne ion is controllable during the address periods without significant increases in the capacitive load of the address electrodes.

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FEM Analysis of spring back in bending process of center plate for molten carbonate fuel cell (용융탄산염 연료전지용 금속분리판 굽힘 공정의 유한요소 해석을 통한 스프링백 분석)

  • Lee, C.H.;Ryu, S.M.;Yang, D.Y.;Kim, Y.J.;Kang, D.W.;Chang, I.G.;Lee, T.W.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2009.05a
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    • pp.220-223
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    • 2009
  • Metallic bipolar plate for molten carbonate fuel cell(MCFC) is composed of the shielded slot plate and the center plate. Among these, the center plate plays an important role in gas sealing. Therefore, manufacturing of the center plate is considered one of the key issues in MCFC. The center plate is manufactured by bending process. In bending process, springback and recoiling are two main problems. By using the modified punch shape with 'step', springback and recoil are reduced. The aim of this article is to find the effect of modified punch shape. So, the bending stress along thickness direction and material direction were investigated using FEM.

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