• 제목/요약/키워드: KatA

검색결과 59건 처리시간 0.034초

Response of Bioluminescent Bacteria to Sixteen Azo Dyes

  • Lee, Hwa-Young;Park, Sue-Hyung;Gu, Man-Bock
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제8권2호
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    • pp.101-105
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    • 2003
  • Recombinant bioluminescent bacteria were used to monitor and classify the to xicity of azo dyes. Two constitutive bioluminescent bacteria, Photobacterium phosphoreum and Es-Cherichia coli, E, coli GC2 (lac::luxCOABE), were used to detect the cellular toxicity of the azo dyes. In addition, four stress-inducible bioluminestent E. coli, DPD2794 (recA::luxCDABE), a DNA damage Sensitive strain; DPD2540 (fabA::luxCDABE), a membrane damage sensitive strain; DPD2511 (katG::luxCDABE), an oxidative damage sensitive strain; and TV1061 (grpE::luxCDABE), a protein damage sensitive strain, were used to provide information about the type of toxicity caused by crystal violet, the most toxic dye of the 16 azo dyes tested. These results suggest that azo dyes result in serious cellular toxicity in bacteria, and that toxicity monitoring and classific ation of some azo dyes, In the field, may be possible using these recombinant bioluminescent bacteria.

Growth of Escherichia coli in Iron-enriched Medium Increases HPI Catalase Activity

  • Zaid, Tarrik;Srikumar, Trivandrum Sukumaran Nair;Benov, Ludmil
    • BMB Reports
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    • 제36권6호
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    • pp.608-610
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    • 2003
  • Escherichia coli has two catalases, HPI and HPII. HPI is induced during logarithmic growth in response to low concentrations of hydrogen peroxide. This induction is OxyR-dependent. On the other hand, HPII is not peroxide-inducible but is induced in entry to the stationary phase. We demonstrate here that E. coli displayed higher HPI catalase activity when compared to the cultures that were grown in a normal medium, if grown in a medium supplemented with iron-citrate. Iron supplementation had no effect on HPII catalase. This increase of HPI activity was OxyR-independent and not observed in a ${\Delta}fur$ mutant. The physiological significance of the increase of HPI activity is unclear, but it appears that the katG gene that codes for HPI catalase is among the genes that are regulated by Fur.

노인의 하지 근력강화운동이 기립균형에 미치는 영향 (Effects of Exercise Training on Strength and Balance for the Elderly)

  • 김택훈;오동식
    • 한국전문물리치료학회지
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    • 제7권1호
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    • pp.32-37
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    • 2000
  • The purpose of this study is to determine the effects of exercise training on the strength and balance ability of the elderly. The results of this study are: 1) After 8 weeks exercise training, knee muscle strength showed a significant increment. 2) After exercise training, forward functional reach and balance index of KAT 2000 showed a significant increment. Results indicate that strengthening exercise can result in improved muscle strength and balance in the elderly. Further studies are required to show long-term effects of exercise training on the elderly.

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내외용 쓰개류에 대한 고찰 (A Study on the Women's Veil of Yi-Dynasty)

  • 강순제
    • 한국의류학회지
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    • 제2권2호
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    • pp.277-286
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    • 1978
  • The history of the women's veil which was used to hide their face in Yi-Dynasty was discussed. The similar fashion of wearing veil was popular in T'ang China as Myok-li and Yoo-Mo, then it might have influenced the fashion of the women's dress of the Silla Dynasty. It was also found out that the custom was most popular in Koryo and then continued to Yi-Dynasty. The confucianism of Yi-Dynasty requested very strict moral obligation toward women that veil had to be worn by them whenever going out. It might not be their popular fashion but obligation. There were several types of veil so called; Neoul(라원) Jangott(장의) Suege-China(쓰개치마) Chun-eue(천의) Sak-kat(삿갓) Chun-mo(천모)etc., which were used as a part of formal dress for women. From the late 1930, when the new society started to accept women's activities, the customs of wearing veil had been vanishing from the women's dress.

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IT제조업의 수직적 분업에 관한 연구 (A Study on the vertical international specialization for Information Technology manufacturing)

  • 박종명;조성갑
    • 한국정보처리학회:학술대회논문집
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    • 한국정보처리학회 2010년도 추계학술발표대회
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    • pp.966-968
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    • 2010
  • 최근 정보통신기술의 발전과 세계화의 진전으로 IT제조업의 생산 효율화를 통한 비용절감의 방안으로 각 생산공정의 국제분업이 이루어지게 되었다. 국제분업을 통해 국가는 비교우위의 생산요소에 집적을 통해 효율화를 이루게 된다. 하지만 이러한 국제분업을 통해 기술의 양극화 현상이 나타나게 되고 국제분업구조에서 기술중진국의 비교우위부문의 확보가 새로운 과제로 부상되었다. 따라서 기술중 진국이 장기적으로 국제분업구조에서 기술부문의 비교우위확보를 위해 민간부문과 공공부문에서 동시에 이루어져야한다.

IT아웃소싱에서 Service Desk 운영 방안 연구 (A Study on the Service Desk of Information Technology Service Outsourcing)

  • 박종명;조성갑
    • 한국정보처리학회:학술대회논문집
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    • 한국정보처리학회 2010년도 춘계학술발표대회
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    • pp.680-682
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    • 2010
  • IT서비스의 아웃소싱을 통해 기업은 핵심역량에 집중하므로써 비용절감을 통한 경쟁력확보를 위해 노력해왔다. 서비스데스크는 단순히 고객의 불만사항이나 장애처리를 담당하는 영역을 넘어 IT아웃소싱에서 고객사와 IT서비스 제공사의 단일접점 뿐만 아니라 IT서비스제공사간의 원활한 의사소통을 통해 협업을 이루어가고 있다. 신세계 이마트의 무인점포시스템과 에릭슨코리아의 토털IT아웃소싱의 사례 중심으로 서비스데스크의 역할과 기능을 살펴본다.

Simulating Rotation Measure toward High Galactic Latitude

  • ;류동수;김종수
    • 천문학회보
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    • 제36권2호
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    • pp.116.2-116.2
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    • 2011
  • We investigate Faraday rotation measure (RM) toward high Galactic latitudes due to the Galactic magnetic field (GMF). The Galactic contribution to RM comes from the global component and the turbulent component of the GMF, and we newly model the latter by incorporating data of MHD turbulence simulations. We find that for the turbulent velocity of ~50 km/s, the standard deviation of the RM due to the GMF toward high Galactic latitudes is close to the observed value, several rad m^{-2}. Yet, the predicted second-order structure function (SF) has values substantially smaller than the observed ones at separation angles of <~ several degree. This suggests that the intergalactic magnetic field (IGMF) significantly contributes to RM toward high Galactic latitudes, particularly at small angular scales. Our work proposes a strategy for surveys to explore the IGMF with LOFAR, ASKAP, MeerKAT, and SKA.

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감각통합훈련이 20대 대학생들의 균형능력에 미치는 효과 (Effects of Sensory Integration Training on Equilibrium Ability in the College Students of the Twenties)

  • 한종만;이우형
    • 대한정형도수물리치료학회지
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    • 제16권1호
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    • pp.37-42
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    • 2010
  • Purpose : The purpose of this study was to evaluate the influence of sensory integration training on equilibrium ability in the college students of the twenties. Methods : The sensory integration training group(male: n=10, female: n=9) was measured an equilibrium ability by Kinesthetic Ability Trainer(KAT) at pre-intervention and post-intervention in 4weeks. Results : Mean value of eye close both leg standing and eye close one leg standing tests of the sensory integration training group were significantly reduced among the intervention period(p<.05). Conclusion : We were found that sensory integration training could reduce mean value of eye close both leg standing and eye close one leg standing tests and improve a balance for the college students of the twenties.

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Modeling the Galactic Rotation Measure at High Galactic Latitude Using MHD Turbulence Simulations

  • ;류동수;김종수
    • 천문학회보
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    • 제36권1호
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    • pp.85.1-85.1
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    • 2011
  • Recently, Faraday rotation measure (RM) at high Galactic latitude has been investigated, partly to explore the Galactic magnetic fields and partly to study the extragalactic magnetic fields. The Galactic contribution to RM comes from the global component as well as the turbulent component. So far the turbulent field was used to be analytically modeled with a Kolmogorov-type power spectrum. Here, we present the initial results of the work where the turbulent field is modeled using data of MHD turbulence simulations. Our work is intended to be applied to simulations of RM surveys with LOFAR, ASKAP, MeerKAT, and SKA.

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알코올에 대한 Escherichia coli, Clostridium acetobutylicum, Saccharomyces cerevisiae의 반응 (Cellular Responses to Alcohol in Escherichia coli, Clostridium acetobutylicum, and Saccharomyces cerevisiae)

  • 박주용;홍천상;한지혜;강현우;정봉우;최기욱;민지호
    • Korean Chemical Engineering Research
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    • 제49권1호
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    • pp.105-108
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    • 2011
  • 유가의 급등과 화석 연료에 의한 온난화 현상은 재생 가능한 대체 연료에 대한 필요성이 요구되었다. 수송용 바이오 연료를 비교하였을 때 에탄올보다 높은 알코올 경우 휘발유와 비슷한 장점을 갖는데 그 이유는 높은 에너지 밀도와 낮은 흡습성을 갖기 때문이다. 이러한 이유로 미생물의 발효는 지속적인 에너지를 얻을 수 있는 잠재적 생산자라 할 수 있다. 본 연구에서는 생물학적으로 생산되는 알코올 성분에 대하여 두 종의 세균과 한종의 효모인 Escherichia coli와 Clostridium acetobutylicum 그리고 Saccharomyces cerevisiae를 이용하여 바이오 알코올에 대한 세포 성장 정도와 함께 미생물내에 스트레스 반응 유전자들의 분석을 실시하였다. 분석한 알코올은 에탄올과 부탄올이며, 이들의 농도별 세균의 성장속도와 산화적 손상에 민감하게 반응하는 katG 유전자, 생물막 손상에 민감하게 반응하는 fabA 유전자, 단백질 손상에 민감하게 반응하는 grpE 유전자, 유전자 손상에 민감하게 반응하는 recA 유전자의 반응여부를 분석하였다. 그 결과, 에탄올과 부탄올 중 부탄올의 세포 독성이 더 높게 관찰되었으며, 부탄올의 경우 생물막 손상을 유발하는 세포내 독성효과를 지니고 있음을 확인하였다.