• Title/Summary/Keyword: Karyotypic analysis

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Intraspecific Polymorphism and Classification of Paeonia Iactiflora Based on the Giemasa C-banding Patterns

  • Seo, Bong-Bo
    • Journal of Plant Biology
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    • v.39 no.3
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    • pp.203-207
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    • 1996
  • On the basis of karyotypic analysis performed by conventional staining and Giemas C-banding technique, cytological relationship was inferred for 21 lines of Paeonia lactiflora Pal. cultivated in Korea. It was very difficult to infer their organized karyotypic classification system using the composition of somatic chromosomes involving sat-chromosomes, relative length of chromosomes, arm ratio and karyotypic formulae by conventional staining. From the distribution and number of Giemsa C-bands on the chromosomes b and c, 21 lines can be subclassified into 5 groups. It seems that the karyotypic polymorphism is observed in 21 lines of cultivated P. lactiflora because peony mainly propagates by outbreeding.

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Variations in Karyotypic Characteristics of Different Breed Groups of Water Buffaloes (Bubalus bubalis)

  • Bondoc, O.L.;Flor, M.C.G.T.;Rebollos, S.D.N.;Albarace, A.G.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.3
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    • pp.321-325
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    • 2002
  • Karyotype analysis was carried out on blood samples of 30 water buffaloes belonging to different breed groups (i.e. Philippine Carabao (PC), Indian Murrah (IM), Bulgarian Murrah (BM), "$F_1$ 50% IM-50% PC", "$F_1$ 50% BM-50% PC" and "75% IM-25% PC"), using the modified Leucocyte Culture Technique. The modal chromosome numbers of the PC, "$F_1$ 50% IM-50% PC", "$F_1$ 50% BM-50% PC", IM, BM and "75% IM-25% PC" were 2n=48, 49, 49, 50, 50 and 50, respectively. The water buffalo chromosomes are mostly acrocentric (79.67%) and the remainder submetacentric (20.33%). Results of the ordinary least square analysis showed significant breed effects (p<0.01) on other karyotypic characteristics (i.e. relative length, arm ratio and centromeric index). Significant correlation between karyotypic characteristics and some animal performance traits were also found. The significant correlation values imply that karyotypic characteristics can be used as important criteria to select potentially productive young water buffaloes. In the future, more production and reproduction traits from non-institutional herds should be included in the analysis to reveal meaningful correlations with various karyotypic characteristics.

Karyotypic Analysis of Chicken Grunt, Parapristipoma trilimeatum, Small Yellow Croaker, Larimichthys polyactis, and Brown Croaker, Miichthys miiuy

  • Park, In-Seok;Gil, Hyun Woo
    • Development and Reproduction
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    • v.23 no.1
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    • pp.73-78
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    • 2019
  • To clarify the cytogenetic features of the chicken grunt, Parapristipoma trilineatum, small yellow croaker, Larimichthys polyactis, and brown croaker, Miichthys miiuy, in Jeju, Korea, karyotype analysis was performed. The karyotype of chicken grunt and brown croaker consists of 24 pairs of telocentrics [2n=48, fundamental number (FN)=48], and small yellow croaker consists of 3 pairs of submetacentrics and 21 pairs of telocentrics (2n=48, FN=54). No evidence of polymorphism, including aneuploidy or sex-related heteromorphic chromosomes, was found in any specimens examined in this study.

karyotypic Analysis Based on Heterochromatin Distribution in Allium fistulosum and Allium ascalonicum (Allium fistulosum과 Allium ascalonicum에서 헤테로크로마틴 분포에 의한 핵형분석)

  • 서봉보
    • Journal of Plant Biology
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    • v.18 no.3
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    • pp.92-100
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    • 1975
  • The present study demonstrates karyotype based on H-patterns of A.fistulosum and A. ascalonicum using Giemsa technique. The results obtained in this study are summarized as follows: I). Karyotypic analysis of A. fistulosum is 6VII+$JII^t+JII$ and that of A. ascalonicum collected from a local farm in the suberbs of Taegu city clearly heterozygous as $13V+J_1^t+J_2+i. ii$). The heterochromatin of both species is generally located distally in both arms of chromosomes and each chromosome type possesses some variations on H-patterns. iii). The percentage of heterochromatin to total chromosome length in cell is about 14.6% in A. fistulosum, 12.8% in A. ascalonicum. The number of bands is revealed about 38 in A. fistulosum and 33 in A. ascalonicum. Also in the amounts of chromocenters per nucleus, the former is somewhat more than the latter.

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Karyotypic Analysis and Physical Mapping of rRNA Gene Loci in Persicaria tinctoria (쪽의 핵형분석과 rRNA 유전자의 염색체상 위치)

  • Choi, Hae-Woon;Lee, Sang-Hoon;Kim, Soo-Young;Bang, Jae-Wook
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.3
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    • pp.195-198
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    • 2008
  • Karyotypic analysis and FISH (fluorescence in situ hybridization) with 45S and 5S rRNA genes were carried out in Persicaria tinctoria H Gross. The somatic metaphase chromosomes were ranged from 2.25 ${\mu}m$ to 1.50 ${\mu}m$ in length. Chromosome number was 2n = 4x = 40 with the basic number of x = 10. The chromosome complement of the species consisted of 16 pairs of metacentrics (chromososomes 1,2,3,4,6,7,8,9, 10, 11, 12, 13, 15, 18, 19 and 20) and 4 pairs of submetacentrics (chromosome 5, 14, 16 and 17). The karyotype formula was K(2n) = 4x = 32 m + 8 sm. In FISH analysis, three pairs of 45S rRNA gene loci on the terminal region of submetacentrics (chromosomes 5, 16 and 17) and two pairs of 5S rRNA gene loci on the centromeric region of metacentrics (chromosomes 9 and 11) were detected, respectively.

Karyotypic Analysis of Four Labrid Fishes from Korea (한국산 놀래기과 어류 4종의 핵형분석)

  • Park, In-Seok;Kim, Hyung-Bae;Lee, Young-Don
    • Korean Journal of Ichthyology
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    • v.7 no.1
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    • pp.79-83
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    • 1995
  • Karyotypic analysis was performed for Labridae fishes, Pseudolabrus japonicus, Halicho eres tenuispinis, H. poecilopterus and Pteragogus flagellifera from coastal area of Cheju Island in Korea. The chromosome numbers(Karyotypes) were 42(4M+24SM+14ST, A), 48(2M+2SM+44ST, A), 48(2M+46ST, A) and 42(4M+24SM+14ST, A) in P. japonicus, H. tenuispinis, H. poecilopterus and P. flagellifera, respectively. Heteromorphic sex chromosome was not found in both sexes of each Labridae fishes. However, large satellites were located on the largest subtelocentrics in P. japonicus and P. flagellifera.

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Morphological Characteristics and Karyotypic Analysis of Aster spathulifolius According to Native Area

  • Yoon Pyung-Sub;Park Hye-Mi;Kim Dong-Min;Kim Hyun-Hee
    • Plant Resources
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    • v.8 no.3
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    • pp.244-249
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    • 2005
  • The growth charateristics and karyotypes of Aster spathulifolius collected from 5 sites including coastal and island region on the Korean peninsula, were analysed. Several morphological characteristics of the plants such as leaf length, leaf width, top internode, medium internode, spike branching, flower diameter, number of petal, leaf color, leaf form, stem and leaf hair, viscosity, and serration of the plants were distinctly different depending on the native region from which they were collected. Karyotypic analysis showed that the chromosome number was all diploid (2n=18), with one pair of submetacentric satellite chromosomes. The chromosome composition included 7 pairs of metacentric chromosomes and 2 pairs of submetacentric chromosomes in all plants. However, chromosome order and the ranges of the chromosome lengths were a little different from plant to plant according to their native growing regions. The plants from Geoje-Do especially showed large differences in the chromosome lengths between the longest and the shortest compared to the plants from other places. This results provide important data to support the classification of the species into several sub-species.

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Karyological Variation of Callus-derived Regenerants in Allium victorialis var. platyphyllum

  • Seo, Bong-Bo;Lee, Seon-Hee;Park, Jae-Hong;Kim, In-Sun;Song, Seung-Dal
    • Journal of Plant Biology
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    • v.39 no.4
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    • pp.231-235
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    • 1996
  • The calli obtained from the bulbs of A. victorialis var. platyphyllum on MS based medium containing 2 mg/L 2, 4-D and 1mg/L BA. Plants from calli were regenerated on MS basal media supplemented with combinations of NAA and four kinds of cytokinin, BA, zeatin, kinetin and 2iP, and with only each cytokinin. The good response of shoot regeneration was observed in the media with combinations of NAA and BA or zeatin, and with only BA or zeatin. Shoot regenerating response in the medium with combinations of NAA and BA or zeatin, and with only BA or zeatin. Shoot regerating response in the medium with combinations of NAA and BA or zeatin was about two times higher than that in the mediuim with only BA or zeatin. From the karyotypic analysis of the regenerated plants, abnormal diploids, aneuploids and mixoploid with structural aberrations were investigated. The somaclonal variants (AV 16-04, AV 13-03) were shown the considerable differences from normal diploid regenerant (AV 18-03) in the external morphology.

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Karyotype Analysis of Five Iris Species Native to Korea (한국 자생 붓꽃과 식물 5종의 핵형분석)

  • Park, Young-Wook;Kim, Dong-Ming;Hwang, Yoon-Jung;Lim, Ki-Byung;Kim, Hyun-Hee
    • Journal of Plant Biotechnology
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    • v.33 no.1
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    • pp.39-43
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    • 2006
  • A detailed karyotype analysis was carried out in five Korean native Iridaceae species; Iris pseudoacorus, I. pallasii. var. chinensis, I. tectrum, I. dichotoma and Belamcanda chinensis. Chromosome compositions of the five species showed that they have different karyotypic formulae; I. pseudoacorus 2n=34=10m+16smn+8st including two pairs of satellite chromosomes, I. pallasii var. chinensis 2n=40=26m+12sm+2st including two pairs of satellite chromosomes, I. tectrum 2n=30=14m+16sm including five pairs of satellite chromosomes, I. dichotoma 2n=32=22m+10sm including two pairs of satellite chromosomes, and B. chinensis 2n=32=20m+10sm+2st including one pair of satellite chromosomes. These results will supplement the previous cytogenetic reports in Iridaceae species and enhance our understanding on the genetic structure, which will be useful in clarifying the unique characteristics of each species for practical breeding programs for horticultural and pharmaceutical purposes.

The Karyotypic Analysis of Niwaella multifasciata Wakiya et Mori(Cobitidae), An Endemic Species in Korea (한국(韓國) 특산종(特産種) 수수미꾸리, Niwaella multifasciata Wakiya et Mori(Cobitidae)의 핵형분석(核型分析))

  • Nam, Myung-Mo;Yang, Hong-Jun;Chae, Byung-Soo
    • Korean Journal of Ichthyology
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    • v.3 no.1
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    • pp.36-41
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    • 1991
  • The karyotype of the Niwaella multifasciata, endemic species to the Nakdong River in Korea, is reported for the first time. The sample species, Niwaella multifasciata collected from the Milyang River and the Nam River which are tributaries of the Nakdong River, has a diploid complement of 50 chromosomes composed of 10 metacentric, 28 submetacentric, 12 subtelocentric chromosomes, and 88 arm numbers. The arm number of Niwaella multifasciata was larger than any other species of Cobitidae. Sexual dimophism and intraspecific polymophism of the chromosomes were not observed in this study.

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