• Journal of Forest and Environmental Science
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• v.35 no.4
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• pp.272-280
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• 2019

Forest medicinal resources, which constitute one of the non-timber forest products, have been regarded as healthy and highly valued products. To meet the increasing demand of the medicinal resources, it is necessary to improve the propagation methods of medicinal plants. In vitro propagation not only allows an opportunity for propagating plants in large numbers but also allows for enhancing the quality and quantity of the desired functional component of a plant by altering the growth factors, such as medium, carbon source, and plant growth regulators influence plant. There have been several studies of in vitro propagation methods, such as axillary bud culture, shooting, and embryogenesis, on Kalopanax septemlobus, Eleutherococcus sessiliflorus, Hovenia dulcis, and Schisandra chinensis in Korea between from 2000 through 2010. Furthermore, there have been attempts to proliferate callus and plantlets for producing useful natural compounds by using bioreactors. Here, we provide an account of the in vitro propagation methods of medicinal trees in Korea based on a review of several micropropagation studies.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.388-395
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• 2015

Somatic embryogenesis is as an excellent technology for potential use in plant mass production, germplasm conservation, or genetic engineering. We examined the effect of cold storage using 3 embryogenic callus lines with different levels of embryogenesis competence derived from immature zygotic embryo cultures of Kalopanax setemlobus. Somatic embryo induction, germination and plant conversion were evaluated after 1, 3 and 6 months storage at $4^{\circ}C$ in the dark. Most cold-stored embryogenic calli formed somatic embryos normally even after 6 months; however, the induction rate was gradually decreased by increasing the storage period. The most competent line tended to show a slight decline in somatic embryo induction rate, as compared with other lines after cold storage. In general, cold storage resulted in reduced somatic embryo germination and plant regeneration, although 93% somatic embryo germination and 91% plant conversion were achieved regardless of the storage period. Cold storage led to cell browning and degradation. Additionally, the cell structures were confirmed by the aceto-carmine and evans blue dye evaluation. Collectively, our results showed that embryogenic callus of K. septemlobus could be preserved at $4^{\circ}C$ without subculture for 6 months, and suggested the need for storage of relatively more competent embryogenic calli lines to support somatic embryo induction.

• Journal of the Korean Wood Science and Technology
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• v.38 no.5
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• pp.391-398
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• 2010

In this study, the figure of domestic hardwood was observed using the naked eye and the possibility of substituting imported wood with domestic one in the making woodcraft or furniture was investigated. It was known from the investigation on 8 different hardwoods that there were various figures such as cloud-shaped figure, blister figure, crotch figure, bird's eye figure, etc. in Zelkova serrata wood and the beautiful pigment figure in Diospyros Kaki wood. There were the unusual figures such as wavy grain figure, fiddle back figure, etc. in Cedrela sinensis wood and was clear ray-fleck figure in Quercus variabilis wood. There were also various figures such as wavy grain figure, blister figure, pigment figure, etc. in Sophra japonica, Tilia amurensis, Castanea crenata, Kalopanax septemlobus wood, etc. It is thus that distinguished figures appeared in each kind of wood can be used for craft, sculpture, furniture, and interior material. These kinds of figures can also be used for instrument, toy, stationery, life article material and souvenir, etc. And it can be expected that they will replace the imported wood has been mainly used to make the woodcraft or furniture until now.

• Journal of Korean Society of Forest Science
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• v.97 no.3
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• pp.229-236
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• 2008

The objectives of this study were to investigate inputs and pH values of stemflow of bulk precipitation and 5 tree species (Deciduous species : Quercus mongolica, Betula costata, Kalopanax septemlobus, Fraxinus rhynchophylla, Plantation species : Larix kaempferi), in Mt. Joongwang, Pyongchang-gun, Gangwondo, from June to October in 2004 and 2005. The amount of stemflow during study period was the greatest in Q. mongolica (9.9 mm), and B. costata (7.5 mm), K. setemlobus (5.9 mm), L. kaempferi (3.8 mm), F. rhynchophylla (3.2 mm), respectively. Stemflow of each species increased with bulk precipitation. Increment with bulk precipitation was smaller in 2004 when the intense bulk precipitation occurred more. pH values of stemflow was the highest in F. rhynchophylla (5.91), and K. setemlobus (5.64), B. costata (5.80), Q. mongolica (5.56), L. kaempferi (5.25), respectively. Generally, pH values of stemflow of all species increased with bulk precipitation pH value, and lower than that (6.39).

• Korean Journal of Environment and Ecology
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• v.21 no.2
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• pp.113-119
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• 2007

To investigate the vegetation structure at Orimok of Hallasan national park, 28 plots$(2,000m^2)$ were surveyed by random sampling method. Carpinus tschonoskii was a major woody species in the studied area in Orimok, and Quercus gorsseserrata, Quercus serrata, and Quercus mongolica were partly occupied. High negative correlations were shown between C. tschonoskii and Ilex macropoda, Lindera erythrocarpa, Quercus mongolica Comus walteri and Symplocos coreana, and relatively high positive correlations were proved to exist between Acer pictum var. mono and Carpinus cordata, Quercus gorsseserrata Ilex macropoda and Lindera erythrocaypa, Q. mongolica Abies nephrolepis and Prunus sargentii, Kalopanax septemlobus Taxus cuspidata and Symplocos coreana. Species diversity(H') was 0.771, and it was relatively low numerical value compared to that of other national parks.

• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.343-356
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• 2010

Plant micropropagation techniques include bud cultures using apical or axillary buds, organogenesis through callus culture or adventitious bud induction, and somatic embryogenesis. In Korea Forest Research Institute (KFRI), the first tissue culture trial in woody plant was initiated from the bud culture of hybrid poplars (Populus alba x P. glandulosa) in 1978. Since then several mass propagation techniques have developed from conifer and hardwood species, resulting in allowing practical application to Poplars, Birches and some oak species. In addition, useful micropropagation and genetic resources conservation techniques were established in some rare and endangered tree species including Abeliophyllum distichum. Among various in vitro propagation techniques, somatic embryogenesis is known to be the most efficient plant regeneration system. Since the first somatic embryo induction was reported in Tilia amurensis by KFRI in 1986, various protocols for direct or indirect somatic embryogenesis systems have developed in conifer and hardwood species including Larix leptolepis, Pinus rigida x P. taeda F1, Kalopanax septemlobus and Liliodendron tulipifera, etc. However, most of these technologies have been developed using juvenile tissues, i.e. immature zygotic embryos or mature embryos. Therefore it has been difficult to directly application to tree breeding program due to their unproven genetic background. Recently remarkable progresses and new approaches have been achieved in mature tree somatic embryogenesis. In this article we reviewed several micropropagation techniques, which have been mainly developed by KFRI and recent international progresses.

• Korean Journal of Plant Resources
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• v.29 no.1
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• pp.62-89
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• 2016

The great part of the traditional knowledge on ethnobotanical plants and their uses is gradually vanishing due to industrialization, therefore ethnobotanical studies that explore and preserve the knowledge are in urgent needed before the knowledge are lost ever. This study was conducted to record and conserve the traditional knowledge of botanical taxa in Korea from 2006 to 2014. According to the survey results, derived from 17,328 sheets of 1,771 residents at 868 places, the ethnobotanical plants in Korea consisted of a total 924 taxa; 130 families, 493 genera, 813 species, 7 subspecies, 92 varieties and 12 form. Of them, herbs were 619 taxa (67.0%) and woody plants were 305 taxa (33.0%). 707 taxa (76.5%) out of 924 taxa grow wild, 44 taxa (4.8%) were cultivated wild species, 145 taxa (15.7%) were introduced for cultivation, and 28 taxa (3.0%) were naturalized plants. The analysis of usage for 924 taxa showed that the edible use was the highest with 58.9%, followed by medicinal with 26.9%, material with 3.7% and ornamental with 2.3%, respectively. The leaf of plant was the most useful part, followed by stem and root. Quantitative analysis of the ethnobotanical plants in Korea was performed by the basic values of FC, NU and UR, and indices of CI, RFC, RI and CV. As a result, The CI place Artemisia princeps in first position, followed by Aster scaber, Kalopanax septemlobus. The RFC place A. scaber in first position, followed by A. princeps, Aralia elata. The RI place A. princeps in first position, followed by Pinus densiflora, Morus alba. The CV place A. princeps in first position, followed by P. densiflora, K. septemlobus.

• Korean Journal of Environment and Ecology
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• v.22 no.4
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• pp.453-460
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• 2008

This study conducted an analysis of plant species composition and vegetation at sea cliffs of the uninhabited islets off Ganghwa-gun by applying discontinuous classification method & continuous ordination method of species composition table by correlation. The analysis results are as follows: First, the plant showing the highest appearance frequency was Parthenocissus tricuspidata Planch.(58.3%), followed by Carpinus coreana Nak., Viburnum carlesii Hemsl. and Kalopanax septemlobus Koidz.(37.5%,respectively). As for a plant community, it was found that it consisted of Quercus mongolica community, Tilia mandshurica community, Carpinus coreana community, Sorbus alnifolia community and Pinus thunbergii community; as for the community distribution classified by individual islet, it was revealed that Quercus mongolica community appeared at Sosongdo and Bunjido; Tilia mandshurica community at Sosongdo and Daesongdo; Carpinus coreana community appeared at Woodo, Suribong and Bido; Sorbus alnifolia community at Susido, Sosongdo and Daesongdo, and Pinus thunbergii community at Seokdo and Woodo, respectively. The plant similarity classified by uninhabited islet was found to be the highest at Sosongdo and Daesongdo. This research judged the reason why the typical grassland community of coastal cliffs did not join these islets to be environmental conditions of rock landform where there exists almost no surface soil at most sea cliffs; thus, more detailed study on this was required.

• Journal of Plant Biotechnology
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• v.43 no.1
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• pp.82-90
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• 2016

Most Araliaceae plant species distributed in Korea are economically important because of their high medicinal values. This study was conducted to develop barcode markers from sequence analysis of chloroplast DNA in 14 taxa of Araliaceae species grown in South Korea. Sequencing of seven chloroplast DNA regions was performed to establish the DNA barcode markers, as suggested by the Consortium for the Barcode of Life (CBOL). From the sequence analysis of chloroplast DNA, we identified specific sequences and nucleotides that allowed us to discriminate among each other 14 Korean Araliaceae species. The sequence in the region of psbA-trnH revealed the most frequent DNA indels and substitutions of all 7 regions studied. This psbA-trnH marker alone can discriminate among all 14 species. There are no differences between Korean and Chinese Panax ginseng in all seven sequenced chloroplast DNA regions. A phylogenetic tree constructed using the seven chloroplast DNA regions revealed that Tetrapanax papyriferus should be classified as an independent clade. The Aralia and Panax genera showed a close phylogenetic relationship. Five species in the Eleutherococcus genus were more closely related to Kalopanax septemlobus than to any Panax species.

• Korean Journal of Ecology and Environment
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• v.48 no.2
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• pp.122-128
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• 2015

This study aims to figure out the vegetation status and characteristics of the spatial distribution in Taeanhaean National Park. As a result, vegetation communities were Pinus thunbergii community, Pinus densiflora community, Pinus thunbergii-Pinus densiflora community, Camellia japonica community, Carpinus turczaninowii community, Carpinus turczaninowii-Pinus thunbergii community, Kalopanax septemlobus community, Koelreuteria paniculata community, Robinia pseudoacacia community, and sand dune and coastal wetland vegetation including Vitex rotundifolia community, Elymus mollis community, Calamagrostis epigeios community, Ischaemum anthephoroides community, Zoysia sinica community, Suaeda maritima community, and Phragmites communis community. Landscape types in actual vegetation map were Pinus thunbergii community (3.92%), Pinus densiflora community (1.40%), Robinia pseudoacacia community (0.05%), sand dune and coastal wetland vegetation (0.11%), field (0.46%), seashore (0.24%), thinning area (0.08%), bareground (0.16%), and sea (93.58%). Area of Pinus thunbergii community among vegetation type was $14.797km^2$ (3.92%) and the largest. Stratification structure and species composition of Pinus thunbergii community distributed in the seashore were different as a result of disturbance and human use. On the other hand, we assumed that succession will take place to the potential natural vegetation that is Quercus serrata and Quercus dentata in undisturbed Pinus thunbergii community. Meanwhile, Erechtites hieracifolia was occupied in understory of Pinus thunbergii community around the seashore and Diodia teres was invaded to sand dune vegetation. They may affect on natural species negatively. Therefore, monitoring and management plans are necessary.