• Korean Journal of Pharmacognosy
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• v.35 no.1 s.136
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• pp.1-5
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• 2004

Eight compounds were isolated from the n-BuOH soluble fraction of the leaves of Weigela subsesillis. On the basis of spectral data, they wεre identified as $kaempferol-O-3-{\alpha}-L-(3-O-acetyl)\;rhamnopyranosyl-7-O-{\alpha}-L-rhamnopyranoside$ (1), sutchuenoside A (2), kaempferitrin (3), astragalin (4), kaempferol 7-O-rhamnoside (5), scopolin (6), farxin (7), kaempferol 3-O-{\alpha}-L-rhamnosyl-7-O-{\beta}-D-glucoside (8), respectively.

• Korean Journal of Food Science and Technology
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• v.53 no.2
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• pp.213-217
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• 2021

Benign prostate hyperplasia (BPH) is one of the most common elderly disease, and because of prolonged incubation period and many side effects of medication or surgical interventions, the use of dietary phytochemicals is considered as an effective measure for prevention of BPH. The purpose of this study is to investigate the mechanism of inhibition effect for BPH by flavonoids such as baicalein and kaempferol. BPH cells were collected through biopsy from patients with PSA of 4 or higher, followed by primary culture. In vitro experiments were conducted to evaluate mitochondrial respiration, intracellular reactive oxygen species (ROS) level and expression of inflammatory markers, genes, and anti-oxidants. In conclusion, baicalein and kaempferol have been demonstrated to inhibit BPH through lowering ROS, thereby reducing inflammation triggers, and reduced inflammation. This study is expected to be helpful in the development of flavonoids that have a clinical effect on suppressing BPH.

• Journal of the Korean Wood Science and Technology
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• v.32 no.6
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• pp.43-49
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• 2004

Three flavonoids, quercetin, taxifolin, and kaempferol were isolated from the woods of Liriodendron tulipifera. Their structures were determined by spectral analysis. Based on 1, l-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity method, the antioxidative activities of three isolated compounds and their acetates were measured in order to search for natural antioxidants. The IC₅₀ of quercetin (1), taxifolin (2), and kaempferol (3) were 3.6, 3.9, and 4.1 ㎍/㎖, respectively.

• Journal of Life Science
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• v.29 no.12
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• pp.1371-1377
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• 2019

Oxidative stress induced by the over-production of reactive oxygen species (ROS) in the brain is the most common cause of neurodegenerative diseases such as Alzheimer's. In the present study, we investigated the protective effects of flavonoids and their glycosides, namely kaempferol, kaempferol-3-O-glucoside, quercetin, and quercetin-3-β-D-glucoside, against H₂O₂-induced oxidative stress in the C6 glial cells. The H₂O₂-treated glial cells exhibited decreased cell viability and increased ROS production when compared with normal cells. However, cells treated with each of the four flavonoids/glycosides demonstrated significantly increased viability and suppressed ROS production when compared with the H₂O₂-treated control group. These results indicate that flavonoids/glycosides attenuate oxidative stress induced by H₂O₂ in C6 glial cells. To confirm the protective molecular mechanisms, we measured pro-inflammatory factors such as inducible nitric oxide synthase, cyclooxygenase-2, and interleukin-1β. H₂O₂ treatment was seen to elevate these factors and decrease IκB-α in the C6 glial cells, while the flavonoids/glycosides induced a down-regulation of the pro-inflammatory factors and increased IκB-α, indicating a neuroprotective effects through attenuation of the inflammation. In particular, quercetin and its glycoside showed a higher neuroprotective effect than the kaempferol treatments. These results suggest that these flavonoids and their glycosides could be promising therapeutic agents for neurodegenerative diseases via the attenuation of oxidative stress.

• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.35-41
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• 2017

One of the most important species, Brassica rapa encompasses a variety of commercial vegetables, such as the Chinese cabbage, pak choi and oilseed crops. The LP08 of yellow sarson (Brassica rapa ssp, tricolaris) have a distinct morphology, with yellow seed color and a unique tetralocular ovary. LP21 of pak choi (Brassica rapa ssp, chinensis) have a dark brown seed color and bilocular ovary. In this study, we generated double haploid plants by crossing the LP08 (maternal variety) and LP21 (paternal variety), using microspore culture. A total of 66 accessions with various morphological characteristics were used for content analysis of flavonoids. The three flavonoids, quercetin, naringenin and kaempferol, showed differing contents in the two crossing parents. The Chinese cabbage type 'Chiifu' was used as the control. The highest accumulation of total flavonoids was observed in LP08. The lowest mean total flavonoids were found in 'Chiifu'. Among the 66 DH accessions, the quercetin contents of 18 accessions showed higher content than LP08. Kaempferol content was also high, and was found to be 79.7% of the total flavonoid content. Naringenin content was low at 2.8%, and was not detected in 22 accessions. Interestingly, the quercetin content positively correlated with the kaempferol content. These results can be used to identify genetic locus and genes related to useful traits. Phenotypic analysis of 66 DH accessions can further be used for natural selection of good breeding materials in B. rapa.

• Journal of Life Science
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• v.28 no.5
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• pp.509-516
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• 2018

Five phenolic compounds were isolated from the ethyl acetate fraction of leaves from Stewartia koreana, and their nitric-oxide (NO) inhibitory activities were measured to identify the major active constituents responsible for the efficacy of the extract against inflammatory reactions. These five compounds were quercetin (1), quercitrin (2), hyperin (3), quercetin-3-O-(6"-O-galloyl)-${\beta}$-D-galactopyranoside (4), and kaempferol 3-O-[2",6"-di-O-(trans-p-coumaroyl)]-${\beta}$-D-glucopyranoside (5). Among the separated compounds in the EtOAc fraction, compounds 4 and 5 were isolated for the first time, and no study has yet reported their anti-inflammatory effects. The compounds were identified by spectroscopic analysis, and the isolated compounds showed significant NO inhibitory effects in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Compound 5 showed the most potent inhibitory effect (63.35% inhibition) against LPS-induced NO production compared to that of compound 1 (17.17%), compound 2 (5.0%), compound 3 (3.92%), and compound 4 (6.32%) at $10{\mu}g/ml$ concentration. NO production was inhibited by suppressing the protein expression of inducible nitric-oxide synthase in LPS-stimulated RAW 264.7 macrophages. These results indicate that kaempferol 3-O-[2",6"-di-O-(trans-p-coumaroyl)]-${\beta}$-D-glucopyranoside might be the major active compound responsible for the anti-inflammatory effects of S. koreana.

• Proceedings of the Ginseng society Conference
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• 1988.08a
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• pp.141-146
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• 1988

Dwarf ginseng (Panax trifolius L.) is a member of the ginseng family (Araliaceae). which is indigenous to North America and is distributed from Southern Canada to the Northern United States. In total. nine compounds were isolated from the leaves of Dwarf gineng. Of these. four were identified as flavonoids and five were found to be ginsenosides. Two of the flavonoids were identified to be kaempferol-3. 7-dirhamnoside and kaempferol-3-gluco-7-rhamnoside. Four of the ginsenosides were identified as notoginsenoside-Fe. ginsenoside-Rd. ginsenoside-Rc and $ginsenoside-Rb_1$ The common aglycone of these ginsenosides was shown to be (20S)-protopanaxadiol. The identification of flavonoids and ginsenosides from the root. stem. leaf. flower and fruit of Dwarf ginseng was detected by Two-Dimensional Thin-Layer Chromatography (2D-TLC) and High Performance Liquid Chromatography (HPLC). The quantitation of flavonoids and ginsenosides from the root. stem. leaf. flower and fruit of Dwarf ginseng and related species such as Korean gineng (Panax ginseng C.A. Meyer) and American ginseng (Panax quinquefolium L.) was analyzed by HPLC only. Three flavonoids (Kaempferol derivatives) labelled compound 1 $(10.8\%)$, compound 3 ($2.8\%$), and compound 4 ($8.4\%)$ were found in the root of Dwarf ginseng but not found in the roots of Korean ginseng and American ginseng. This is the first time that flavonoids have been found and identified in roots of the ginseng family (Araliaceae).

• Natural Product Sciences
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• v.11 no.1
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• pp.13-15
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• 2005

Phytochemical study of the methanolic extract of Flaveria trinervia (Asteraceae) leaves has led to the isolation of three constituents characterised as 3,5,7,4' tetrahydroxy-6-methoxy flavone (6-methoxy kaempferol), oleanolic acid and ${\beta}-sitosterol-{\beta}-D-glucoside$. The identities of the compounds were confirmed by the physical and spectroscopic data and by comparison with authentic samples.

• Korean Journal of Pharmacognosy
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• v.26 no.3
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• pp.239-243
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• 1995

Six flavonoids were isolated from the leaves of Aralia continentalis. Their structures were characterized as kaempferol, quercetin, 6'-O-acetyl astragalin, astragalin, trifolin and hyperoside by chemical and spectroscopic evidences. This is the first isolation from this plant.

• Bulletin of the Korean Chemical Society
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• v.27 no.3
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• pp.357-358
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• 2006