• Title/Summary/Keyword: KM12

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Transport System of Specific Neutral Amino Acids in Suspension-Cultured Cells (현탁배양 세포내에서 특수 중성 아미노산의 수송)

  • Bong-Heuy CHO
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.4
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    • pp.201-206
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    • 1994
  • The influx of glycine, valine, alanine, and histidine was inhibited by all tested neutral amino acids competitively and the reciprocal inhibitory studies showed the neutral amino acids possess the same transport system as neutral amino acids process to the same catalytic site of one carrier to each other, The molecules of histidine were transported actively as a neutral form through the neutral amino acid transport system but were not transported as a charged form. The Km values of the neutral amino acid transport system have been divided into three different category on basis of the affinity to the carrier, below 0.1mM, etween 0.1ImM-0.5mM and above 0.5mM. The $V_{max}$ was between $3.12{\mu}mole{\cdot}h^{-1}{\cdot}g$ fresh $weight^{-1}\;-\;15.1\;{\mu}mole{\cdot}h^{-1}{\cdot}g$ fresh $weight^{-1}$. Neutral amino acids cotransported with one $H^{+}per$ one molecule and one $K^{+}-efflux$ per one molecule for charge compensation. Histidine cotransported with proton per one molecule, however the movement of cotransported proton can't detectable because of the release of proton from the charged molecules of histidine in the medium.

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Assessment of Rainfall-Sediment Yield-Runoff Prediction Uncertainty Using a Multi-objective Optimization Method (다중최적화기법을 이용한 강우-유사-유출 예측 불확실성 평가)

  • Lee, Gi-Ha;Yu, Wan-Sik;Jung, Kwan-Sue;Cho, Bok-Hwan
    • Journal of Korea Water Resources Association
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    • v.43 no.12
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    • pp.1011-1027
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    • 2010
  • In hydrologic modeling, prediction uncertainty generally stems from various uncertainty sources associated with model structure, data, and parameters, etc. This study aims to assess the parameter uncertainty effect on hydrologic prediction results. For this objective, a distributed rainfall-sediment yield-runoff model, which consists of rainfall-runoff module for simulation of surface and subsurface flows and sediment yield module based on unit stream power theory, was applied to the mesoscale mountainous area (Cheoncheon catchment; 289.9 $km^2$). For parameter uncertainty evaluation, the model was calibrated by a multi-objective optimization algorithm (MOSCEM) with two different objective functions (RMSE and HMLE) and Pareto optimal solutions of each case were then estimated. In Case I, the rainfall-runoff module was calibrated to investigate the effect of parameter uncertainty on hydrograph reproduction whereas in Case II, sediment yield module was calibrated to show the propagation of parameter uncertainty into sedigraph estimation. Additionally, in Case III, all parameters of both modules were simultaneously calibrated in order to take account of prediction uncertainty in rainfall-sediment yield-runoff modeling. The results showed that hydrograph prediction uncertainty of Case I was observed over the low-flow periods while the sedigraph of high-flow periods was sensitive to uncertainty of the sediment yield module parameters in Case II. In Case III, prediction uncertainty ranges of both hydrograph and sedigraph were larger than the other cases. Furthermore, prediction uncertainty in terms of spatial distribution of erosion and deposition drastically varied with the applied model parameters for all cases.

Characteristics on sea level variations in the South Indian Ocean (남인도양의 해수면 변화 특성)

  • 윤홍주
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.5 no.6
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    • pp.1094-1103
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    • 2001
  • According to standard procedures as defined in the users handbook for sea level data processes, I was compared to Topex/Poseidon sea level data from the first 350days of mission and Tide Gauge sea level data from the Amsterdam- Crozet- Kerguelen region in the South Indian Ocean. The comparison improves significantly when many factors for the corrections were removed, then only the aliased oceanic tidal energy is removed by oceanic tide model(11) in this period. Making the corrections and smoothing the sea level data ()ver 60km along-track segments and the Tide Gauge sea level data for the time series results in the digital correlation and RMS difference between the two data of c=-0.12 and rms= 11.4cm, c=0.55 and rms=5.38cm, c=0.83 and rms=2.83cm, and c=0.24 and rms=6.72 for the Amsterdam, Crozet and Kerguelenplateau, and Kerguelen coast, respectively. It was also found that the Kerguelen plateau has a comparisons due to propagating signals(the baroclinic Rossby wave with velocity of -3.9 ~-4.2cm/sec, period of 167days and amplitude of 10cm) that introduce temporal lags(${\gamma}$: 10~30days) between the altimeter and tide gauge time series. The conclusion is that on timescales longer than about 10days the RMS sea level errors are less than or of the order of several centimeters and are mainly due to the effects of currents rather than the effects of stories(water temperature, density) and winds.

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Hydrologic Utilization of Radar-Derived Rainfall (I) Optimal Radar Rainfall Estimation (레이더 추정강우의 수문학적 활용 (I): 최적 레이더 강우 추정)

  • Bae Deg-Hyo;Kim Jin-Hoon;Yoon Seong-Sim
    • Journal of Korea Water Resources Association
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    • v.38 no.12 s.161
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    • pp.1039-1049
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    • 2005
  • The objective of this study is to produce optimal radar-derived rainfall for hydrologic utilization. The ground clutter and beam blockage effects from Mt. Kwanak station (E.L 608m) are removed from radar reflectivities by POD analysis. The reflectivities are used to produce radar rainfall data in the form of rain rates (mm/h) by the application of the Marshall-Palmer reflectivity versus rainfall relationship. However, these radar-derived rainfall are underestimated in temporal and spatial scale compared with observed one, so it is necessary to hire a correction scheme based on the gauge-to-radar (G/R) statistical adjustment technique. The selected watershed for studying the real-time correction of radar-rainfall estimation is the Soyang dam site, which is located approximately 100km east of Kwanak radar station. The results indicate that adjusted radar rainfall with the gauge measurement have reasonal G/R ratio ranged on 0.95-1.32 and less uncertainty with that mean standard deviation of G/R ratio are decreased by $9-28\%$. Mean areal precipitation from adjusted radar rainfall are well agreed to the observed one on the Soyang River watershed. It is concluded that the real-time bias adjustment scheme is useful to estimate accurate basin-based radar rainfall for hydrologic application.

Simulations of Changes in Wind Field Over Mountainous Terrains Using WRF and ENVI-met Numerical Models (WRF와 ENVI-met 수치 모델을 이용한 산악지형의 바람장 변화 모사)

  • Won, Myoungsoo;Han, Seonho
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.15 no.1
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    • pp.17-25
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    • 2013
  • In this paper we interpreted the changes in wind field over complex mountainous terrains. The results of our study can be applied for predicting the direction of fire spread and for establishing strategies for fire prevention. The study area is bounded by $12{\times}12$ km domains of the Samcheok's long-term ecological research (LTER) site located in the east coast, in which a large-fire had occurred from 7 to 13 April 2000. Because of the area's complex topography, we compared the result of the Weather Research and Forecasting (WRF) mesoscale model with those observed by four automated weather stations. The WRF simulation overestimated the wind speed by 5 to 8 m/s (~200%) in comparison with those from four automated weather stations. The wind directions observed by the AWSs were from various directions whereas those from WRF model were mostly west wind at all stations. Overall, the simulations by the WRF mesoscale models were not appropriate for the estimation of microscale wind fields over complex mountainous areas. To overcome such inadequacy of reproducing the wind fields, we employed the ENVI-met model over Samcheok's LTER site. In order to test the model's sensitivity with the terrain effects, experimental simulations were conducted with various initial conditions. The simulation results of the ENVI-met model showed a reasonable agreement in wind speeds (about 70% accuracy) with those of the four AWSs. Also, that the variations in wind directions agreed reasonably well with changes in terrain effect. We concluded that the ENVI-met model is more appropriate in representing the microscale wind field over complex mountain terrains, which is required to predict fire spread and to establish strategies for forest fire prevention.

Studies on the Trial Manufacture of Telesounder and its Application (2) - Remote Observations of Fishes Entering in the Gape Nets With Wings - (원격어군탐지기의 시작 및 그 응용에 관한 연구 -II -낭장망 입망어군의 원격관측-)

  • 이원우
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.31 no.1
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    • pp.54-62
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    • 1995
  • In order to telemeter the behavior and distribution of fishes. the telesounder was manufactured and it was examined to verify its performance and effects in the gape nets with wings located around Gokunsan islands. Yellow Sea. on August 8. 18~19 and September 8~9, 1994. The behavior of fish entering the net was observed with the buoy station at sea which was installed at the entrance of the gape nets with wing and its echosignal was transmitted remotely to the base station on ship at distance of about 1 km away from the buoy station at sea. The fishes catched by the gape nets with wings were 12 species and Anchovy(Engraulis japonica), Beka squid(Loligo beka), Herring(Harengula zunasi) was 95% of total catches. The images of color display monitor at the base station on ship were very well coincident with the records of fish finder from the buoy station located at sea. When the current velocity was less than 0.6 kt and slight fluctuation, the fishes entered into the gape net were much more in comparision with over 1.0kt and heavy fluctuation, and then the average swimming depth was about 4 m. The catches per one hauling was about 10~30 kg and when the current velocity was too weak and the current direction did not coincident with the direction of net or the current velocity was too strong, the catches was a few. It is concluded that telesounder system is very useful for investigating the distribution and the swimming behavior of fishes entering in the gape nets with wings.

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Spring season City Traffic accident prevention police activity -centering Gwangju province police agency - (봄철 교통사고 예방 경찰활동 - 광주지방경찰청을 중심으로 -)

  • Kang, maeng-jin
    • Proceedings of the Korea Contents Association Conference
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    • 2009.05a
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    • pp.549-558
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    • 2009
  • By the statistics of traffic safety public corporation as of Feb. 2009 year, in domestic 16,861,965 cars were registered and in this Gwangju is registered by 476,149 cars. Except Ulsan, compare to other metropolitan city, vehicles numbers are fewer. Then, as of 2009. 4. 9. by the announcement of National land and marine ministry, the operation distance of passenger cars of Gwangju metropolitan city is 40.9 km per 1 day and the most highest in the whole country. On the one hand, by the materials of traffic safety public corporation, from 2007. 1. 1 to 2007. 12. 31 for a year, traffic accidents of Korea were 211,662. By this, 6,166 people were death, 335,906 people were wounded. This mean about 17 people death and 920 people wounded per day. In Gwangju metropolitan city, last year 8,231 traffic accidents took place and 138 people were death and 13,533 people were wounded. This while government did various traffic policy to reduce traffic accidents and some degree were progressive. But still the actual circumstances is that disgrace of traffic undevelopment country can't take off. Moreover, the concern for the traffic safety of feeling of general people is very deficient. In this research, with the help of Gwangju province police station. analysed traffic accidents reality of 2009 year March and this is going to look around spring season traffic accidents prevention activities and spring season means from March to June.

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Introduction of Satellite Remote Sensing Technologies to Korea Coast Guard (해양경찰청 위성활용 방안)

  • Yang, Chan-Su;Oh, Jeong-Hwan
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2011.11a
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    • pp.154-155
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    • 2011
  • 2010년 천리안위성의 성공적인 발사에 따라 인공위성의 활용에 대한 기대가 커지고 있다. 천리안 해양관측위성(GOCI)이외에 아리랑 2호가 현재 운용중인 우리나라 위성들이다. 가까운 시기에 아리랑 5호(2011년 말), 아리랑 3호(2012년), 아리랑 3A호(2013년)가 발사될 예정이다. 즉, 해양적용을 위한 위성환경은 이제부터 준비되고 있다고 볼 수 있다. 대외적으로 보면, 인공위성 자원은 아주 많다. 문제는 이와 같은 자원을 어떻게 활용할 것인가 인데 이의 활용 기술 개발적 측면에서는 많이 소홀한 것이 사실이다. 전세계적으로 이 시스템 개발을 위한 치열한 경쟁이 진행 중에 있다. 이미 소말리아 주변 감시체계는 많은 부분을 위성에 의지하고 있다. 우리나라에서 최초로 위성활용 가능성을 보여준 사건이 허베이스피리트호 원유유출 사고이다. 이 사고는 2007년 12월7일 아침 7시6분경 서해안 만리포 북서쪽 10km 해상에서 크레인을 적재한 1만1800t급 바지선이 정박 중인 홍콩 선적 유조선 허베이 스피리트호(14만6000t급)와 부딪치면서 발생했다. 이와 같은 기름 유출 사고의 경우, 유출 범위를 정확하게 이해하는 것이 중요하다. 거의 준비된 상태가 아님에도 불구하고 12월 8일 아침 최초로 유출된 기름을 모습을 보여주는 위성이미지(광학위성)가 얻어졌다. 하지만 이와 같은 자료가 관련 전문가가 이용할 수 있기까지 많은 시간이 소용되었고, 이 정보를 전달할 수 있는 방법도 없었다. 사실 단순한 이미지가 아니라 지리정보체계를 가진 오염정보를 제공할 방법도 준비도 되어 있지 못한 상황이었다. 본 발표를 통하여, 허베이스피리트호 사고뿐만 아니라, 2011년 6월부터 수개월간 지속된 발해만 오염사고 적용 등 다양한 사례 소개를 하고, 이를 기반으로 해양경찰청에서 업무활용을 위한 방안을 제시한다. 먼저, 해경청의 주요 임무인, 경비, 수색구조, 오염대응 분야별로 현황 분석을 수행하였다. 또한 국외사례에 대한 조사를 한 후, 최종 인공위성 원격탐사기술의 해경청 도입방안에 대한 설계를 실시하였다. 국제적으로 인공위성을 이용한 해양 경비, 수색구조, 오염 모니터링기술 개발이 이루어지고 있으며, 유럽 국가는 시범도입을 진행 중에 있다. 유럽해사안전국(EMSA)은 해양경비 및 수색구조를 위한 선박통항 및 보고 서비스와 오염대비대응(Pollution Preparedness and Response, PPR) 위성 서비스를 회원국에 제공하고 있다. 해양경찰청 임무 수행뿐만 아니라, 해양영토 관리적 측면에서 첨단 위성장비 활용, 선진국형 해상경비 패러다임의 전환 필요성이 크다고 할 수 있다.

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Preparation and Properties of Coimmobilized Glucose Oxidase-Catalase (Glucose Oxidase와 Catalase의 동시 고정화 제품과 성질)

  • Lee, Suk-Hee;Uhm, Tai-Boong;Cho, Sook-Ja;Byun, Si-Myung
    • Applied Biological Chemistry
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    • v.27 no.3
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    • pp.180-186
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    • 1984
  • For the study of glucose oxidase(GOD) and catalase(CAT) coimmobilization system, the enzymes were obtained from Penicillium spp., PS-8, and the strain itself was used as an immobilizing matrix. To separate glucose oxidase and catalase after the ammonium sulfate fractionation of the culture broth, DEAF-cellulose column was used and its activity yield was 54 and 34%, respectively. Both enzymes were immobilized on the cell matrix, followed crosslinking with 2.5% glutaraldehyde for 12hr. In the determination of efficiencies of GOD and CAT of dual, mixed and soluble enzyme systems, the dual immobilized one w-as superior to those of the soluble or mixed ones. In the comparison of pH profiles, the dual and mixed types showed broader maximum pH ranges than the soluble type. Varying CAT/GOD ratio of the dual system, the higher the ratio showed the broader activity profile. In the comparison of apparent $K_m$ of GOD only and CAT/GOD=10, they were $7.1{\times}10^{-2}$ and $5.1{\times}10^{-2}M$. Their activation energies showed 3.98kcal/mole/deg for GOD only and 2.98kcal/mole/deg for CAT/GOD=10.

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Spatial Characteristics of Travelling Merchants of Apartment's New Periodic Market in Cheongiu City, Korea (청주시 지역 아파트 신정기시 이동상인의 공간적 특성)

  • Han, Ju-Seong
    • Journal of the Economic Geographical Society of Korea
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    • v.9 no.3
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    • pp.341-357
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    • 2006
  • Recently new periodic markets formed in large apartment areas where consumers live. Before, in the case of peasant periodic markets travelling merchants, consumers and producers met in specific places on decided dates. Closing time of apartment's new periodic markets is later than that of peasant periodic markets, and the number of travelling merchants is fewer than that of peasant periodic markets. The average number of apartment and household for a new periodic market is about ten and 920 respectively, and if neighboring apartment household are included, the number is larger. Apartment's new periodic markets in Cheongju city is included in Daejeon market area. The types of regional trip of travelling merchants can be divided into one round trip of two or three neighboring dongs and larger sphere of more than 4 dongs. The larger sphere round trip consists of one type combining the southeastern, southern and southwestern regions, and the other type combining southeastern and southwestern regions. About 85 percent of travelling merchants visit the periodic market 5 days in a week; about 12 percent of travelling merchants choose to visit on Saturday; only 2 percent of travelling merchants visit 4 days in a week.

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