• The Korean Journal of Food And Nutrition
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• v.3 no.1
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• pp.57-68
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• 1990

In order to obtain the fundamental informations on cellulase of Trichoderma viride QM 9414 for its production and utilization, some physico-chemical properties of the enzyme were reviewed. When T. viride QM 9414 was cultured on wheat bran medium, filter paper-disintegrating and carboxymethyl cellulose-saccharifying activity were increased with the cell growth, and thereafter CMC-saccharifying activity was kept on almost the same leved while filter-paper disintegrating activity was decreased sharply. And B-glucosidase was formed maximally on the late stationary phase of growth. The crude cellulase of cell-free extracts was purified by (NH4)2SO4 fractionation, Sephadex-G 200 column chromatography and DEAE Sephadex A-50 column chromatography. Filter paper-disintegrating, CMC-saccharifying and B-glucosidase activity were purified 10-fold, 47-fold and 38-fold, respectively. The crude enzyme was proved to be a complex of three different enzyme proteins which were showing filter paper-disintegrating, CMC-saccharifying and B-glucosidase activity. The optimal pH of the three enzyme components was alike pH 4.0, and the optimal temperature for CMC-saccharifying, filter paper-disintegrating and B-glucosidase activity were 4$0^{\circ}C$, 45$^{\circ}C$ and 5$0^{\circ}C$ respectively. The Km and Vmax values of CMC saccharifying activity for CMC were 0.485% and 3.10, and the Km and Vmax vallues of B-glucosidase for PNPG were 0.944$\times$10-3M and 0.097, respectively. The Km and Vmax values of filter paper-disintegrating activity for Avicel were determined to be 0.09% and 0.178, respectively. B-Glucosidase activity was competitively inhibited by glucose, and the Ki value for this enzyme was 3.54$\times$10-3M, CMC saccharifying activity was found to be greatly inhibited by cellobiose.

• The Journal of the Korea Contents Association
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• v.11 no.12
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• pp.103-111
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• 2011

The research has increased the role of physical activity in promoting health and preventing chronic disease. Estimating algorithm of physical activity energy expenditure was implemented by using a tri-axial accelerometer motion detector of the SVM(Signal Vector Magnitude) of 3-axis(x, y, z). COUNT method has been proven through experiments of validity Freedson, Hendelman, Leenders, Yngve was implemented by applying the SVM method. A total of 10 participants(5 males and 5 females aged between 20 and 30 years). The activity protocol consisted of three types on treadmill; participants performed three treadmill activity at three speeds(3, 5, 8 km/h). These activities were repeated four weeks. Customized estimating algorithm for energy expenditure of physical activities were implemented with COUNT and SVM correlation between the data.

• Bulletin of the Korean Chemical Society
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• v.28 no.5
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• pp.772-776
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• 2007

In order to study the role of residue in the active site of glutathione S-transferase (GST), Arg13 residue in human GST P1-1 was replaced with alanine, lysine and leucine by site-directed mutagenesis to obtain mutants R13A, R13K and R13L. These three mutant enzymes were expressed in Escherichia coli and purified to electrophoretic homogeneity by affinity chromatography on immobilized GSH. Mutation of Arg13 into Ala caused a substantial reduction of the specific activity by 10-fold. Km GSH, Km DCNB and Km EPNP values of R13A were approximately 2-3 fold larger than those of the wild type. Mutation of Arg13 into Ala also significantly affected I50 values of S-methyl-GSH that compete with GSH and ethacrynic acid, an electrophilic substrate-like compound. These results appeared that the substitution of Arg13 with Ala resulted in significant structural change of the active site. Mutation of Arg13 into Leu reduced the catalytic activity by approximately 2-fold, whereas substitution by Lys scarcely affected the activity, indicating the significance of a positively charged residue at position 13. Therefore, arginine 13 participates in catalytic activity as mainly involved in the construction of the proper electrostatic field and conformation of the active site in human GST P1-1.

• Mycobiology
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• v.31 no.1
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• pp.42-45
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• 2003

For transformation of Pleurotus ostreatus, two novel vectors, pPhKM1 and pPhKM2, were constructed, using the regulatory sequences of the P. sajor-caju $\beta$-tubulin gene(TUB1) and the ble gene encoding phleomycin binding protein. pPhKM1 contains ble fused to the TUB1 promoter and the Schizophyllum commune GPD terminator. pPhKM2 contains ble fused to the promoter and terminator regions of P. sajor-caju TUB1. To confirm phleomycin-resistance activity, each vector was cotrans-formed with pTRura3-2 into the P. ostreatus homokaryotic $ura^-$ strain. The transforming DNA was stably integrated into the genomic DNA. Subsequently, phleomycin resistance was conferred on wild-type dikaryotic P. ostreatus by transformation with pPhKM1 or pPhKM2. This transformation system generated stable phleomycin-resistant transformants.

• Journal of rehabilitation welfare engineering & assistive technology
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• v.6 no.1
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• pp.1-8
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• 2012

Estimating algorithm of physical activity energy expenditure was implemented by using a tri-axial accelerometer motion detector of the SVM(Signal Vector Magnitude) of 3-axis(x, y, z). A total of 33 participants(15 males and 18 females) that performed walking and running on treadmill at 2 ~ 11 km/h speeds(each stage increase 1km/h). Algorithm for energy expenditure of physical activities were implemented with $VO_2$ consumption and SVM correlation between the data. Algorithm consists of three kinds and hip, wrist, waist and hip can be used to apply.

• Journal of Sasang Constitutional Medicine
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• v.34 no.3
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• pp.41-49
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• 2022

Background Abdominal obesity, a major public health concern, is related to many health problems. In addition, it is influenced by individual characteristics. We investigated sleep quality and physical activity (PA) as risk factors for abdominal obesity, according to the Sasang constitutional medicine. Methods In this cross-sectional study, we analyzed data from 5,221 community-based participants. Sleep quality and PA were measured using structured questionnaires, and abdominal obesity was classified according to waist circumference. Sasang constitution (SC) was classified as Taeeumin (TE), Soeumin (SE), or Soyangin (SY) type, using an established SC questionnaire. Chi-square test and logistic regression analysis were performed to access the association of sleep quality and PA with abdominal obesity in individuals stratified according to the SC types. Results The percentage of poor sleep quality and inactive PA was the highest in the SE type, and the higher prevalence of abdominal obesity was found in the TE type. After adjusting for variables, inactive PA was associated with abdominal obesity in the TE type (OR=1.694, 95% CI=1.42-2.021), and in the SE type, abdominal obesity was associated with poor sleep quality (OR=1.688, 95% CI=1.091-2.611) and low PA (OR=2.127, 95% CI=1.163-3.89). Moreover, the combination of these two factors were also significantly associated with abdominal obesity in the TE and SE types. Conclusion Abdominal obesity was associated with sleep quality and PA, and these results were different in each SC type. Taking various associated lifestyles and individual characteristics in consideration may contribute to better management of abdominal obesity in clinical practice.

• Microbiology and Biotechnology Letters
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• v.10 no.3
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• pp.211-215
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• 1982

Streptomyces griseorubiginosus var. soyoensis previously identified, produced two kinds of antifungal antibiotics, trans-cinnamamide and another new substance. The latter was identified to be a new substance of tetraene family by establishment of UV, IR, NMR, mass spectra and chemical reactions and rotatively named as Tetraene KM-A. Through an antimicrobial activity test using serial agar dilution method, Tetraene KM-A showed strong growth inhibitory activity against fungi and yeasts, but not against procaryotes tested. The inhibitory action of Tetraene KM-A on fungi was remarkably ineffective when some of sterols were added to the cultural media. $LD_{50}$ of the Tetrene KM-A to mice and rats by intravenous injection were 84.3 and 90.4 mg/kg respectively. $LD_{50}$ to mice by oral feeding was 1503mg/kg.

• Design Convergence Study
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• v.14 no.5
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• pp.157-169
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• 2015

In this study, three axillary crutches(Mobilegs, KM crutch, Pro In-motion) were tested with 12 healthy male subjects by measuring the EMG activity of the latissimus dorsi and gastrocnemius and the user-perceived levels focusing on the comfort and ease of use. The result showed that the EMG activity of the gastrocnemius was significantly reduced during ambulation using Mobilegs and Pro In-motion compared with the ambulation using KM crutch, a conventional crutch. However, no statistically significant difference was found for EMG activity of the latissimus dorsi. Subjects perceived the Mobilegs to be more comfortable and easy to use than the other crutches. Theses result may delivered mostly from the characteristics of spring-loaded axillary pad and ergonomic crutch handle. The results indicate that ambulation using the spring-loaded crutch is much easy with asking less exertion at gastrocnemius. In addition, the characteristics of crutch design, such as embedded spring, makes muscles to consume less energy. When the reasonable structure and form of the axillary pad and crutch handle are applied herein, the ambulation using the crutches are perceived to comfort.

• Journal of the Korean Society of Food Science and Nutrition
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• v.20 no.3
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• pp.209-213
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• 1991

To evaluate the effect of methanethiol on the activity of xanthine oxidase in both liver and serum, the rats intraperitoneally received methoanethiol. Injection of methoanethiol in rats showed the more decreased xanthine oxidase activity of boty liver and serum than that of the control group. Concomitantly, the xanthine oxidase activity in livers preincubated with methanethiol was decreased in vitro. The xanthine oxidase in livers preincubated with the methoanethiol also showed more increased Km and similar Vmax value than those of the control. These results suggest that the methanethiol may induce a change in substrate binding affinity of xanthine oxidase.

• Knowledge Management Research
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• v.10 no.4
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• pp.209-223
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• 2009

Knowledge management(KM) provides a means for organizations to interact with large groups of individuals, and as such, provides an ideal way for government bodies to interact with citizens. Although many private organization have long recognized the value of KM, the value have overlooked in public sector. The case of KSPO, one of leading public organizations in Korea, shows successful example in which KSPO succeeds in KM to improve working performance.