• The Science & Technology
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• v.10 no.6 s.97
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• pp.47-52
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• 1977

18세기 산업혁명이후부터 약 150여년동안 석탄이 인류의 주「에너지」원으로 이용되어 왔으나, 석유가 발견되고 산업화되면서는 석유가 금세기의 주「에너지」원으로 군림하게 되었다. 인류가 이용가능한 지구상의 「에너지원」(중수색을 제외)은 도합 석유환산 8.15 x 10¹²Kl에 달하고 있는데 이중 석탄이 절반을 넘는 57.7%이고 석유는 겨우 3.1%에 불과하여 석유의 가채년수가 30년이라는 한계에 도달하여, 세계각국은 석유에 대신할 수 있는 새로운 「에너지」원 개발에 힘쓰고 있다. <표 1참조> 그러나 지지부진한 상태이고 또한 제품화된다 하더라도 「코스트」가 석유보다 상당히 높으며, 1985년에 가서도 1차 「에너지」부분에서 차지하는 비중이 2%미만일 것으로 추정되고 있다. 따라서 근년에 와서 지구상의 가장 풍부한 「에너지」원인 석탄의 효율적 이용방법으로서, 석탄의 「개스」화 내지 야화에 대한 각국의 관심도가 정치적으로나, 경제적인 이유를 바탕으로 점차 고조되고 있다. 이 글에서는 최근 미국에서 검토된 바 있는 석탄의 야화방법과 상대적 이용가능성에 대해 알아본다.

• Biodesign
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• v.7 no.1
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• pp.24-27
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• 2019

Pnc1 converts nicotinamide to nicotinic acid to generate NAD⁺ through the Preiss-Handler pathway that is one of the NAD⁺-salvage pathway. By reducing levels of nicotinamide, an inhibitor of the NAD⁺-dependent histone deacetylase Sir2, yeast Pnc1 contributes gene silencing. In this study, to understand the structural features and molecular mechanism of nicotinamidase Pnc1, we overexpressed, purified, and crystallized the N-terminally His₆-tagged Pnc1 protein from Kluyveromyces lactis and obtained X-ray diffraction data at a resolution of 2.2 Å. The crystals of the K. lactis Pnc1 (KlPnc1) belonged to space group P2₁2₁2₁ with unit cell parameters a=38.5, b=77.3, c=83.3, and α=β=γ= 90°. There is one molecule in the asymmetric unit.

• Journal of Korean Society of Steel Construction
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• v.26 no.5
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• pp.485-498
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• 2014

In this paper, vehicle-bridge interaction analysis under a series of moving vehicles to simulate a lane load was performed to estimate impact factor of the main cable, hanger and girder for the selected suspension bridges with 404m and 1545m main span. Korea Bridge Design Code(Limit State Design) was selected for the live model in which KL-510 truck was modeled 6-d.o.f. vehicle and a lane load was simulated by a series of single-axle vehicles. For the 404m main span bridge, hinge-type and floating-type girders at the tower were considered to examine the impact factor according to the connection and supporting type of the girders. The parameters considered herein are the types of live load-a truck only and a truck plus lane load, eccentricity of moving vehicles, road surface roughness and vehicle speed. The road surface roughness was randomly generated based on ISO 8608 and it was applied to the truck only. The impact factors were also evaluated by using the influence line method that is commonly used in cable-supported bridges and compared with those from vehicle-bridge interaction analysis.

• Economic and Environmental Geology
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• v.34 no.2
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• pp.217-226
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• 2001

Stable isotope, paleoceanographic and sedimentological analyses were carried out along the core Ml5612 from the Mid-Atlantic Ridge. Distinct negative ${\delta}^{18}O anomalies punctuate the planktonic isotope records and correlate with the Heinrich-IRD cvents. The IRD layer in the corc contains varying amounts of quartz, K-feldspar, plagioclase, calcite, dolomite and mica, in which detrital carbonate contributes between I and 13% (except H3 and H6). Anomalies are strongest in the N. pachydenna (sin.) isotope record. Systematic changes in the ${\delta}^{18}O offset of G. hul/aides and G. inJlata signify variations in mid-latitude thermocline structure. In conjunction with negative benthic ${\delta}^{13}C anomalies, the data document a stronger contribution of a ${\delta}^{13}C depleted, nutrient-rich water mass during the IRD events. The ${\delta}^{13}C amplitude of > 1 $\textperthousand$ between 25 and 57 ka indicates changes between northern source (NADW) and southern source (AABW) water masses at this site. The IRD layers in the core Ml56l2 are correlative with those from the core S075-26KL and DSDP 609. The IRD layers from the Portuguese margin arc coeval with HI, H2 and H4 of the open North Atlantic. This similarity (and/or synchronicity in both regions may have been resulted from common changes in a North Atlantic thermohaline switch.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.5
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• pp.624-634
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• 1995

Tides at both of northern and southern entrances of Kamag Bay were compared by deploying tide gauges for 18 days during July-August 1994. To examine the response of the inner bay to the tidal waves through both entrances, a current meter was moored at the eastern pan of the inner bay. Current meter mooring failed at the northern entrance (Yosu) while the current data was collected for only 5 days at the southern site (Songdo). Maximum range was 357 and 352 cm at Yosu and Songdo, respectively. Respective amplitudes for M2, S2 Kl O1 tides of 95.5, 48.8, 20.5, 14.0cm at Yosu and 93.6, 47.2, 21.3, 13.1cm at Songdo yielded the form numbers of 0.23 and 0.24, respectively, both of which belong to the predominantly semidiurnal tide, Contributions from the overtides and compound tides were less than $4\%^ at both sites. Differences in Greenwich phase of major partial tides between two sites were negligible. Maximum speed of tidal current was about 100cm/sec at the southern entrance and about 40cm/sec at the inner bay. Residual current speed was 17cm/sec southwestward at the southern entrance and 0.9cm/sec southeastward at the inner bay. Temporal change in current at the inner bay showed that the wind had a significant influence upon the circulation in Kamag Bay.

• KSBB Journal
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• v.6 no.3
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• pp.289-297
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• 1991

The insecticidal protein gene in the pKL-20-1 clone derived from Bacillus thuringiensis serovar. kurstaki plasmid was subcloned in the plant shuttle vector, pGA643. The 7.3 kb fragment was cloned in the BglII and Hpal sites of pGA643 vector and expressed in E. coli S17-1, which produced insecticidal proteins killing Bombyx mori larvae. The clone was named pHL-20. The protoplast formation, calli induction and plantlet regeneration of Nicotiana tabacum was carried out. A tremendous number of mesophyll protoplasts of N. tabacum were formed, up to 7$\times$105 protoplast per ml, for 20 hours in darkness in the enzyme solution of 0.5% cellulase and 0.1% macerosin, pH 5.8. The viabilities of the protoplasts were maintained above 80% for 6 days in the media containing 2mg/1 of NAA and 1mg/1 of kinetin. Calli were induced from the protoplasts and leaves of the N. tabacum on MS medium containing 0.5mg/1 BAP. Under the culture conditions the protoplasts underwent repeated cell division into calli. Plantlets were regenerated from callus cultures derived from protoplast and leaves. Shoots were induced in a medium containing 1mg/1 of BAP.

• Fire Science and Engineering
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• v.16 no.3
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• pp.32-38
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• 2002

The synthesis of $CF_3$I and $C_2F_5I from $CF_3Br$ was studied for the reuse of $CF_3Br$ which is abolished to save the ozone layer of the earth. Reaction experiments were carried out in experimental scale synthesis equipment with catalysts, such as CuI, Kl, $K_2$$CO_3$, KF metal salt/active carbon and alumina support at $400~600^{\circ}C$. Main products of reaction were $CF_3i$ and $C_2F_5I$ with small amounts of $C_2F_5I$, $CF_4$, $CF_2Br_2$ by-products. 7.5wt% KI and $K_2CO_3$over activated carbon catalysts show the highest yield of $CF_3$I and 7.5wt% CuI over alumina catalysts show the highest yield of $C_2F_5i$. And optimal reaction temperature was about $500^{\circ}C$.

• The Korean Journal of Zoology
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• v.39 no.1
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• pp.36-46
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• 1996

An endogenous phenoloxidase (EPO) from earthworm, Lumbricus rubellus, has been purified and characterized. The purified EPO using ammonium sulfate fractionation, Blue-2, Phenyl-, and Q-sepharose chromatography steps was revealed in SDS-PAGE as a single protein banri with Mr. of 59 kl)a. A native strudure of the enzyme was examined with an in situ staining of a nondenatudng-PAGE using DL-dopa as a substrate. The result showed that a single band due to the EPO activity was located siighdy above a standard polypeptide with Mr. of 210 kl)a. These fads indicate that the EPO is an oligomeric enzyme. The presence of a monophenolase activity of the purified EPO, which hydroxylates tyrosine to dopa, was confirmed by observing dopachrome accumulation at 475 nm at PH 8.0 with a typical lag phase during 60 mm. of meausrement. A series of inhibition study has been performed for the enzyme with several divalent cation chelators such as phenyithiourea (Flu), 1, lO-phenanthroline, EDTA, and EGTA. Among them, only V'flj inhibited the enzyme with 1C0.5 of 65 MM, which indicated that copper was critical for the catalysis of EPO. The enzyme was maximally active at 35'C and pH 8.0 when L-dopa to dopachrome conversion was spectrophotometricaily monitored at 475 nm. The apparent Km values of P0 for L-opa were obtained as 1.86 mM and 13.8 mM at pH 6.5 and 8.0, respectively. The catalytic efficiencies at both pH were almost identical [(kat/Km)pH8.0/(kcat/Km)pH6.5 = O.92] while the Vmax at p11 8.0 was 6.6-fold higher than that at pH 6.5. This fact may indicate that pH affeds the catalysis at substrate and/or enzyme-substrate complex level rather than the enzyme itself. Taken together, the EPO was an oligomeric enzyme which did not require proteolysis for its activation. These results also indicated that the enzyme can exist, at least, in part as a latent form In vivo, which might be distinct from the prophenoloxidase activating system. Therefore, it is pertinent to consider that there must be certain regulatory molecules or phenomena in L. rubellus which make the 1,0 in a latent form in vivo before the foreign invasions.

• Journal of Microbiology and Biotechnology
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• v.21 no.6
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• pp.604-612
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• 2011

Beauveria bassiana SFB-205 supernatant can effectively control cotton aphid populations, which is closely associated with its chitinase activity. The present work extends to optimizing a culture medium to produce more efficacious supernatant in flask conditions, followed by scale-up in 7 L, 300 L and 1.2 KL fermentors with the parameter of chitinase. In flask conditions, a combination of soluble starch and yeast extract produced the greatest amount of chitinase (5.1 units/ml) and its supernatant had the highest aphicidal activity. An optimal quantitative combination of the two substrates, estimated by a response surface method, enabled the supernatant to have 15.7 units/ml of chitinase activity and 3.7 ml/l of median lethal concentration ($LC_{50}$) of toxicity against cotton aphid adults in laboratory conditions. In the scale-up conditions, overall supernatant had 25-28 units/ml of chitinase activity. Decrease in pH and limitation of dissolved oxygen (DO) during cultures were significantly related to the yield of chitinase. These results suggest that the substrate-dependent chitinase production can be background information for optimizing a culture medium, and pH and DO are critical factors in maximizing the production in scale-up conditions.

• Korean Journal of Soil Science and Fertilizer
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• v.26 no.4
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• pp.314-333
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• 1993

${\bigcirc}$논에 의할 환경보전 측면의 다양한 공익 기능 때문에 벼 농사가 필연적임. ${\bigcirc}$논의 천연적인 만능댐 기능 - 홍수조절 기능 : 36억톤(춘천댐 총저수량의 24배) - 지하수 함량 : 157.5억톤(전국민 1년간 수돗물 사용량의 2.7배) - 고온 여름철 대기 냉방효과 : 냉방용원유 4천 6백만 kl 해당량 ${\bigcirc}$논의 환경정화 기능 - 토양유실방지 기능 : 토양 1cm 생성에 200년 소요, 유실에 2~3년(2천2백억원) - 대기정화 기능 : 대기중 $CO_2$ 제거 및 산소방출 (5조2천8백억원) - 수질정화 기능 : 논토양과 벼에 의한 수질정화 (5조9천7백억원) ${\bigcirc}$논의 농업적 부가가치 - 유기물 소모적음, 토양산성화의 완화, 연작장해방지, 자연적인 제초효과 - 비료손실감소 및 지하수오염경감, 천연적인 양분공급 (617억원).