KSBB Journal
- Volume 6 Issue 3
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- Pages.289-297
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- 1991
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- 1225-7117(pISSN)
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- 2288-8268(eISSN)
Transfer of Insecticidal Toxin Gene in Plants: 2. Subcloning of B. thuringiensis Insecticidal Protein Gene and Rapid Plantlet Regeneration from Nicotiana tabacum Protoplast and Callus
식물세포에 살충독소유전자의 전이연구: 2. B. thuringiensis 살충독소유전자의 Subcloning과 Nicotiana tabacum의 원형질체와 칼루스로부터 신속재생연구
Abstract
The insecticidal protein gene in the pKL-20-1 clone derived from Bacillus thuringiensis serovar. kurstaki plasmid was subcloned in the plant shuttle vector, pGA643. The 7.3 kb fragment was cloned in the BglII and Hpal sites of pGA643 vector and expressed in E. coli S17-1, which produced insecticidal proteins killing Bombyx mori larvae. The clone was named pHL-20. The protoplast formation, calli induction and plantlet regeneration of Nicotiana tabacum was carried out. A tremendous number of mesophyll protoplasts of N. tabacum were formed, up to 7
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