• Title/Summary/Keyword: KL-6

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The Implementation of a Discrete PI Speed Controller for an Induction Motor (유도전동기용 이상 PI형 속도제어기의 구성)

  • 김광배;고명삼
    • The Transactions of the Korean Institute of Electrical Engineers
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    • v.35 no.1
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    • pp.26-35
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    • 1986
  • In this paper, non-linear state equations for a 3-phase, 220V, 0.4 KW, squirrel cage induction motor have been derived using the d-q transformation and then these equations have been linearized around an operating point by a small perturbation method. Root loci on the s-plane with repect to the changes of slip S and supply frequency f have been studied. Based on the above results, the derived linear state equations have been augmented to the 6th order, including the output velocity feedback and a discrete PI speed controller. Using the new state equations, stability regions on the Kp-Kl plane have been investigated for slip S and sampling time T. In designing a discrete PI controller, the coefficients Kp and Kl around the normal operating point (220V,1,692rpm,60Hz)have been chosen under the assumptions that each response to a perturbation input of reference speed and load torque be underdamped and dominated by a pair of complex poles. Step responses in the experimental system using an Intel SDK-86 and an optimized PWM inverter show satisfactory results that the maximum overshoots and damped frequency are well coincided with ones from the computer simulation.

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Evaluation of the genotoxicity and cytotoxicity in the buccal epithelial cells of patients undergoing orthodontic treatment with three light-cured bonding composites by using micronucleus testing

  • Toy, Ebubekir;Yuksel, Sengul;Ozturk, Firat;Karatas, Orhan Hakki;Yalcin, Muhammet
    • The korean journal of orthodontics
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    • v.44 no.3
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    • pp.128-135
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    • 2014
  • Objective: This study evaluated the cytotoxicity and genotoxicity of fixed orthodontic treatment with three different light-cured orthodontic bonding composites by analyzing micronucleus (MN) formation in the buccal mucosa during a 6-month period. Methods: Thirty healthy volunteers were selected from consecutive patients referred for orthodontic treatment. Equilibrium 2 brackets and molar tubes (Dentaurum) were bonded with three different lightcured orthodontic bonding composites-Transbond XT (3M Unitek), Kurasper F (Kuraray Europe), or GrenGloo (Ormco Corporation)- to all teeth in both arches. Exfoliated buccal epithelial cells were scraped from the middle part of the inner cheeks with sterile cement spatulas before treatment and at 1, 3, and 6 months after treatment. MNs and nuclear alterations, such as karyorrhexis (KR), karyolysis (KL), and binucleated cells (BNs), were scored under a light microscope. Repeated measure ANOVA was used to calculate statistical differences in degenerative nuclear abnormalities. Results: MN rates did not significantly differ among different time points within the same cell type (p > 0.05). In contrast, the number of BNs in buccal epithelial cells significantly increased in all composite groups (p < 0.01, Transbond XT; p < 0.001, Kurasper F and GrenGloo). KL frequency significantly increased between the beginning and end of the study in the Kurasfer F ($0.80{\pm}0.79$ to $1.90{\pm}1.10$; p < 0.05) and GrenGloo ($1.30{\pm}1.06$ to $2.40{\pm}1.08$; p < 0.05) groups. Conclusions: After 6 months of fixed orthodontic treatment with different light-cured composites, morphological signs of cytotoxicity were observed but genotoxic effects were absent.

Health-Promoting Behaviors, Health Literacy, and Levels of Knowledge about Smoking-Related Diseases among Smokers and Non-smokers: A Cross-Sectional Study

  • Celebi, Cihat;Calik-Kutukcu, Ebru;Saglam, Melda;Bozdemir-Ozel, Cemile;Inal-Ince, Deniz;Vardar-Yagli, Naciye
    • Tuberculosis and Respiratory Diseases
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    • v.84 no.2
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    • pp.140-147
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    • 2021
  • Background: For generations, cigarette smoking has presented an important public health concern. This study aimed to compare the health-promoting behavior, exercise capacity, physical activity level, health literacy, and knowledge level of smoking-related diseases between smokers and non-smokers. Methods: The study included 71 smokers (mean age, 32.69±8.55 years) and 72 non-smokers (mean age, 31.88±9.94 years) between the ages of 20 and 60 years. Assessments included a 6-minute walking test (6MWT), Godin Leisure-Time Physical Activity Questionnaire, Health-Promoting Lifestyle Profile II (HPLP-II), Cardiovascular Risk Factors Knowledge Level Scale (CARRF-KL), Asthma/COPD Awareness Questionnaire, the World Health Organization Quality of Life-Bref questionnaire (WHOQoL-Bref [TR]), and Health Literacy Questionnaire (HLQ). Results: The results from the study show that the number of coronary artery disease risk factors measured significantly higher among the smoker group members when compared to that of the non-smoker group members (p=0.001). Smokers had significantly lower %6MWT distance than non-smokers (84.83±4.72 and 93.45±7.16, respectively; p<0.05). However, there were no significant differences between the smokers and non-smokers in terms of physical activity, CARRF-KL, HLQ, WHOQoL-Bref, and HPLP-II subscales or total scores (p>0.05). Additionally, while only forty-one smokers (57.7%) were active, 48 of the non-smoker group was active (66.7%). Conclusion: Smokers suffer greater negative effects to their exercise capacity in comparison to non-smokers. Although smokers and non-smokers have similar levels of health literacy and similar levels of knowledge about cardiovascular disease risk factors and obstructive lung diseases, health professionals could continue to further increase individuals' awareness of smoking-related risk factors and continue to emphasize the importance of physical activity and exercise for protecting cardiopulmonary health.

Cloning of the β-Lactamase Gene from Bacillus sp. J105 and Analysis of Its Expression in E. colis Cells (Bacillus sp. J105 유래 β-lactamase 유전자의 cloning 및 E. coli 내에서의 발현 분석)

  • Kang, Won-Dae;Lim, Hak-Seo;Seo, Min-Jeong;Kim, Min-Jeong;Lee, Hye-Hyeon;Cho, Kyeong-Soon;Kang, Byoung-Won;Seo, Kwon-Il;Choi, Yung-Hyun;Jeong, Yong-Kee
    • Journal of Life Science
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    • v.18 no.11
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    • pp.1592-1599
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    • 2008
  • The $\beta$-lactamase gene was cloned into E. coli DH5$\alpha$ from Bacillus sp. J105 with strong resistance against $\beta$-lactam antibiotics. The chromosomal DNA was partially digested with Sau3AI and ligated to BamHI digested pLAFR3. $\beta$-Lactamase positive clones were obtained by using in vitro packaging kit. The pKL11-${\Delta}4.6$ with $\beta$-lactamase activity was obtained by subcloning of the recombinant plasmid ($\beta$-lac +). The 6.5 kb fragment in the subcloned plasmid was sequenced. The DNA fragment that contains the $\beta$-lactamase gene encodes 309 amino acids. The 0.17 kb upstream region was similar to those of B. thuringinesis and B. cereus with 97% identity. The deduced amino acids sequence was also similar to those of $\beta$-lactamase from B. thuringinesis and B. cereus with 97% and 94% identity, respectively. The phylogenetic tree also showed the relationships of the $\beta$-lactamase gene of Bacillus sp. J105 to genetically related that of other Bacillus strains. Analysis of expression pattern of the pKL11-${\Delta}4.6$ in E. coli, revealed that the secretion efficiency of $\beta$-lactamase was $4{\sim}5%$ and the molecular weight was as same as that of original $\beta$-lactamase (31 kDa) from Bacillus sp. J105.

Cloning and Phylogenetic Characterization of Coat Protein Genes of Two Isolates of Apple mosaic virus from ¡?Fuji¡? Apple

  • Lee, Gung-Pyo;Ryu, Ki-Hyun;Kim, Hyun-Ran;Kim, Chung-Sun;Lee, Dong-Woo;Kim, Jeong-Soo;Park, Min-Hye;Noh, Young-Mi;Choi, Sun-Hee;Han, Dong-Hyun;Lee, Chang-Hoo
    • The Plant Pathology Journal
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    • v.18 no.5
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    • pp.259-265
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    • 2002
  • Apple mosaic virus (ApMV), a member of the genus Ilarvirus, was detected and isolated from diseased 'Fuji' apple (Malus domestica) in Korea. The coat protein (CP) genes of two ApMV strains, denoted as ApMV-Kl and ApMV-K2, were amplified by using the reverse transcription and polymerase chain reaction (RT-PCR) and were analyzed thereafter. The objectives were to define the molecular variability of genomic information of ApMV found in Korea and to develop virus-derived resistant gene source for making virus-resistant trans-genic apple. RT-PCR amplicons for the APMVS were cloned and their nucleotide sequences were determined. The CPs of ApMV-Kl and ApMV-K2 consisted of 222 and 232 amino acid residues, respectively. The identities of the CPs of the two Korean APMVS were 93.1% and 85.6% at the nucleotide and amino acid sequences, respectively. The CP of ApMV-Kl showed 46.1-100% and 43.2-100% identities to eight different ApMV strains at the nucleotide and amino acid levels, respectively. When ApMV-PV32 strain was not included in the analysis, ApMV strains shared over 83.0% and 78.6% homologies at the nucleotide and amino acid levels, respectively. ApMV strains showed heterogeneity in CP size and sequence variability. Most of the amino acid residue differences were located at the N-termini of the strains of ApMV, whereas, the middle regions and C-termini were remarkably conserved. The APMVS were 17.(1-54.5% identical with three other species of the genus Ilarviyus. ApMV strains can be classified into three subgroups (subgroups I, II, and III) based on the phylogenetic analysis of CP gene in both nucleotide and amino acid levels. Interestingly, all the strains of subgroup I were isolated from apple plants, while the strains of subgroups II and III were originated from peach, hop, or pear, The results suggest that ApMV strains co-evolved with their host plants, which may have resulted in the CP heterogeneity.

ID-based Authentication Schemes with Forward Secrecy for Smart Grid AMI Environment (스마트그리드 AMI 환경을 위한 전방 보안성이 강화된 ID기반 인증 기법)

  • Park, Dae-Il;Yeo, Sang-Soo
    • Journal of Advanced Navigation Technology
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    • v.17 no.6
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    • pp.736-748
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    • 2013
  • In this paper, we analyse the vulnerabilities of KL scheme which is an ID-based authentication scheme for AMI network, and propose two kinds of authentication schemes which satisfy forward secrecy as well as security requirements introduced in the previous works. In the first scheme, we use MDMS which is the supervising system located in an electrical company for a time-synchronizing server, in order to synchronize smart grid devices in home, and we process device authentication with a new secret value generated by OTP function every session. In the second scheme, we use a secret hash-chain mechanism for authentication process, so we can use a new secret value every session. The proposed two schemes have strong points and weak points respectively and those depend on the services area and its environment, so we can select one of them efficiently considering real aspects of AMI environment.

Automatic Clustering of Speech Data Using Modified MAP Adaptation Technique (수정된 MAP 적응 기법을 이용한 음성 데이터 자동 군집화)

  • Ban, Sung Min;Kang, Byung Ok;Kim, Hyung Soon
    • Phonetics and Speech Sciences
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    • v.6 no.1
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    • pp.77-83
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    • 2014
  • This paper proposes a speaker and environment clustering method in order to overcome the degradation of the speech recognition performance caused by various noise and speaker characteristics. In this paper, instead of using the distance between Gaussian mixture model (GMM) weight vectors as in the Google's approach, the distance between the adapted mean vectors based on the modified maximum a posteriori (MAP) adaptation is used as a distance measure for vector quantization (VQ) clustering. According to our experiments on the simulation data generated by adding noise to clean speech, the proposed clustering method yields error rate reduction of 10.6% compared with baseline speaker-independent (SI) model, which is slightly better performance than the Google's approach.

Analysis of Shapes of Bending and Draping by the Model Rule (닮음 법칙에 의한 굽힘 및 드레이프 형상의 해석)

  • 서정권;이정욱
    • Journal of the Korean Society of Clothing and Textiles
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    • v.20 no.6
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    • pp.1116-1124
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    • 1996
  • The factors to presume the shapes of bending and draping were examined in this study, by applying the similar phenomenon and theory of analysis. The findings were as followings: 1. The value of deflection angle (f) of deflection curve were almost consistent with those of K number and the shapes of deflection curve were congruent, under the condition of that the values of EI/w are almost similar and the lengths of samples are consistent. 2. The values of drape area, drape coefficient, mean of deflection angle, and $\pi$ number were consistently estimated and the shapes of drape were almost the same, under the condition of that the values of EI/w are similar and the diameter of samples are consistent. 3. In using the samples with different values of EI/w, scale factor, kl, was obtained from the formula, the shapes of bending of the referent samples and compsactive smaples was geometrically similar, which the lengths of samples were 1,1'and were satisfied with the formula, hi: L'11, and their $\pi$ number were also consistent. 4. In applying the samples with different values of Rllw, scale factor (kl) was obtained and then, when semidiameter of samples was adjusted to be satisfied with the formula, k1=L/L, the shapes of draping of referent samples and comparative samples were geometrically similar. Furthermore, their $\pi$ number was also consistent. 5. The shares of bending and draping could be changed in terms of three factors such as the lengths of samples, bending ridigity, and weigths per unit area. $\pi$ number was obtained from theory of similar phenomenon, which was index to presume shapes of bending and the shapes of draping getting from the three factors.

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Genetic Variation and Relationships of Korean Native Chickens and Foreign Breeds Using 15 Microsatellite Markers

  • Kong, H.S.;Oh, J.D.;Lee, J.H.;Jo, K.J.;Sang, B.D.;Choi, C.H.;Kim, S.D.;Lee, S.J.;Yeon, S.H.;Jeon, G.J.;Lee, H.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.11
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    • pp.1546-1550
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    • 2006
  • The purpose of this study was to assess the genetic variation and establish the relationship amongst breeds and strains using 15 chicken specific microsatellite markers. A total of 285 unrelated DNA samples from four Korean native chicken strains (Black strain of Korean native chicken; KL, Red Brown strain of Korean native chicken; KR, Ogol strain of Korean native chicken; KS and Yellow Brown strain of Korean native chicken; KY) and three introduced chicken breeds (F strain of White Leghorn; LF, K strain of White Leghorn; LK, Rhode Island Red; RC and Cornish; CN) were genotyped to estimate within and between breed genetic diversity indices. All the loci analyzed in 15 microsatellite markers showed a polymorphic pattern and the number of alleles ranged from 5 to 14. The polymorphism information content (PIC) of UMA1019 was the highest (0.872) and that of ADL0234 was the lowest (0.562). The expected total heterozygosity (He) within breed and mean number of observed alleles ranged from 0.540 (LF) to 0.689 (KY), and from 3.47 (LK) to 6.07 (KR), respectively. The genetic variation of KR and KY were the highest and the lowest within Korean native strains, respectively. The genetic distance results showed that Korean native chicken strains were separated with the three introduced chicken breeds clustered into another group. The lowest distance (0.149) was observed between the KR and KL breeds and the highest distance (0.855) between the KR and LK breeds. The microsatellite polymorphism data were shown to be useful for assessing the genetic relationship between Korean native strains and other foreign breeds.

In vitro Characterization of Bacteriocin Produced by Lactic Acid Bacteria Isolated from Nem Chua, a Traditional Vietnamese Fermented Pork

  • Pilasombut, Komkhae;Rumjuankiat, Kittaporn;Ngamyeesoon, Nualphan;Duy, Le Nguyen Doan
    • Food Science of Animal Resources
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    • v.35 no.4
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    • pp.473-478
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    • 2015
  • The aim of this study was to screen and In vitro characterize the properties of bacteriocin produced by lactic acid bacteria isolated from Vietnamese fermented pork (Nem chua). One hundred and fifty LAB were isolated from ten samples of Nem chua and screened for bacteriocin-producing lactic acid bacteria. Antimicrobial activity of bacteriocin was carried out by spot on lawn method against both gram positive and gram negative bacteria. One isolate, assigned as KL-1, produced bacteriocin and showed inhibitory activity against Lactobacillus sakei, Leuconostoc mesenteroides and Enterococcus faecalis. To characterize the bacteriocin-producing strain, optimum temperature, incubation period for maximum bacteriocin production and identification of bacteriocin-producing strain were determined. It was found that the optimum cultivation temperature of the strain to produce the maximum bacteriocin activity (12,800 AU/mL) was obtained at 30℃. Meanwhile, bacteriocin production at 6,400 AU/mL was found when culturing the strain at 37℃ and 42℃. The isolate KL-1 was identified as L. plantarum. Antimicrobial activity of cell-free supernatant was completely inhibited by proteolytic enzyme of trypsin, alpha-chymotrypsin and proteinase K. Bacteriocin activity was stable at high temperature up to 100℃ for 10 min and at 4℃ storage for 2 d. However, the longer heating at 100℃ and 4℃ storage, its activity was reduced.