• Title/Summary/Keyword: KINETIC

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Induction of Apoptosis in Human Osteosarcoma Cell Lines(Saos-2) by Single Fraction High Dose Irradiation (고용량 방사선 조사 후 골육종 세포주(Saos-2)의 아포프토시스 발생)

  • Kim, Jae-Do;Chung, So-Hak;Hong, Young-Gi;Choi, Jang-Seok
    • The Journal of the Korean bone and joint tumor society
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    • v.5 no.1
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    • pp.1-8
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    • 1999
  • A single fraction of 50 Gy extracorporeal irradiation, as a modality of limb-sparing operation, has been used to achieve tumor necrosis in osteosarcoma. Although this modality of radiation therapy preserving the mobility of a joint is commonly practiced, the precise knowledge on the radiobiological response of osteosarcoma cell has remained to be elucidated. We therefore observed whether a single high dose irradiation caused apoptosis in osteosarcoma cells and whether the commitment to apoptosis was associated with cell kinetics. We also investigated radiation dose response along the time course for development of apoptosis following single high dose irradiation. The morphologic change in apoptosis was observed by fluorescence with Hoechst 33258 and the degree and the fraction of cells by flow cytometry. Irradiation of osteosarcoma cells with 10, 30 and 50 Gy resulted in chromatin condensation and apoptotic body formation. The degree of apoptosis in osteosarcoma cells was $29.5{\pm}3.56%$, $39.9{\pm}4.83%$ at 24 and 48 hours after 10 Gy irradiation ; $41.1{\pm}3.93%$, $66.9{\pm}5.21%$ at 24 and 48 hours after 30 Gy irradiation ; and $48.0{\pm}3.69%$, $75.6{\pm}4.65%$ at 24 and 48 hours after 50 Gy irradiation. The fraction of cells in cell-cycle kinetic was $39.2{\pm}4.3%$ in G2/M, $22.1{\pm}4.65%$ in G1 at 24 hours after 10 Gy irradiation ; $51.0{\pm}4.3%$ in G2/M, $20.4{\pm}4.7%$ in G1 at 48 hours after 10 Gy irradiation ; $40.3{\pm}3.9%$ in G2/M, $26.1{\pm}4.7%$ in G1 at 24 hours after 30 Gy irradiation ; $59.2{\pm}3.9%$ in G2/M, $5.9{\pm}5.1%$ in G1 at 48 hours after 30 Gy irradiation ; and $44.3{\pm}4.2%$ in G2/M, $21.1{\pm}3.5%$ in G1 at 24 hours after 50 Gy irradiation. The fraction of cells at 48 hours after 50 Gy irradiation could not be observed because of irradiation induced cell death of most of cells. All values for irradiated cells showed accumulation in G2/M phase and reduction in G1 phase, irrespective of irradiation dose. The results suggest that a single fraction of high dose irradiation with 50 Gy results in accumulation of cells at G2/M phase, leading to apoptosis.

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Characteristics and Energy Absorbing Capacity for Rockfall Protection Fence from In-Situ Rockfall Tests (현장 낙석실험을 통한 낙석방지울타리의 특성 및 성능 평가)

  • 구호본;박혁진;백영식
    • Journal of the Korean Geotechnical Society
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    • v.17 no.6
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    • pp.111-121
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    • 2001
  • Rockfall protection fences are used for diminishing rockfall damage in roads side slopes. In order to install the fences in effective way, the conditions of rock slopes and total predicted impact energy of fa11ing rock should be considered. However, the fences have been constructed without any consideration for lithology, height and slope angle of rock slope in Korea. In addition, the information about the performance of the protection fences, which should be evaluated by in-situ test or laboratory test in order to check out the practical use in the field, is not available. Therefore, in design manual for the rockfall protection fence, the specific details for the installation of this type of fence are not provided yet. The full sized rockfall in situ test was carried out for the calculation of falling energy of rock and the evaluation of the maximum energy absorbing capacity of fence. For this test, the rock slopes whose heights are about 20 m and dip angle of 65 degree, have been chosen. This is because those geometries are mean height and slope angle of most road cut slopes along Korean national highway. Based on the preliminary simulation procedure, four different sizes of concrete ball (0.7, 1.3, 2.3 and 4.3 ton) were prepared and flour different types of protection fence were constructed. The results of this test provide information about the maximum energy absorbing capacity of the fence, kinetic energy of rockfall and restitution coefficient, and these results can be utilized in the establishment of rockfall fence design and construction manual.

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Exercise Treatment of Knee Joint After Anterior Cruciate Ligament Reconstruction Operation (전방십자인대 재건술 후 슬관절 운동치료 접근에 관한 고찰)

  • Kim, Yeun-Jung;Jung, Jae-Min;Han, Jin-Tae
    • Journal of the Korean Society of Physical Medicine
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    • v.3 no.1
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    • pp.27-37
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    • 2008
  • Purpose : Today, it enjoys a sports and a leisure where the anterior cruciate ligament(ACL) injury patient increase. The knee joint is important means of human body movement and to do an important duty when it encounters ACL injuries of the knee joint and the many restriction follows in the life which is ordinary. When it is damaged ACL, it comes to determinate that ACL reconstruction and preservation treatment that the according to condition of ligament and knee joint. After ACL reconstruction, that is the fact which already becomes known the exercise treatment advances a recovery and to reduce a sequela. Methods : we researched the method of exercise treatment after anterior cruciate ligament reconstruction operation by journal of science direct and KISS in daecu university. Results : The representative exercise treatment is isometric exercise, isokinetic exercise isotonic exercise and complex exercise but what kind of exercise treatment most is effective, it revealed and support it was not. The method of exercise treatment is very various, so It causes a confusion made to the therapist and patients. So it executes once again it sought the kinetic therapeutic method which is efficient from this research and it tried to observe preceding research after ACL reconstruction. To operation a various the exercise treatments, operation only the treatment which is general compared to it was effective in muscular power and muscle functional improvement. But this like improve despite with the exercise treatment consequence which is continuous from research of most the pain leg compares to the health leg, it appeared the discrepancy which is a muscular power, a muscular endurance and a hypertrophy muscle etc, to the health leg or before operating 100% of muscular power to having gets the many therapy time was the recovery rate. Conclusion : Therefore after ACL reconstruction, it will become the many research continuously which is improve the muscle functional and ROM of the exercise treatment method and From therapeutic site of the patients it does to memorizes knowledge in advance about ACL injuries and the application the isokinetic treatment or exercise program are the set of necessary, frequency and amusement that considers complex what kind of therapeutic exercise becomes accomplished with the patient take care of attention.

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Real-time Observation of Evolution Dynamics of Ge Nanostructures on Si Surfaces by Photoelectron Emission Microscopy (자외선 광여기 전자현미경을 이용한 Si 표면 위에 Ge 나노구조의 성장 동역학에 관한 실시간 연구)

  • Cho, W.S.;Yang, W.C.;Himmerlich, M.;Nemanich, R.J.
    • Journal of the Korean Vacuum Society
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    • v.16 no.2
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    • pp.145-152
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    • 2007
  • The evolution dynamics of nanoscale Ge islands on both Si (001) and (113) surfaces is explored using ultraviolet photoelectron emission microscopy (UV-PEEM). Real-time monitoring of the in-situ growth of the Ge island structures can allow us to study the variation of the size, the shape and the density of the nanostructures. For Ge depositions greater than ${\sim}4$ monolayer (ML) with a growth rate of ${\sim}0.4\;ML/min$ at temperatures of $450-550^{\circ}C$, we observed island nucleation on both surfaces indicating the transition from strained layer to island structure. During continuous deposition the circular islands grew larger via ripening processes. AFM measurements showed that the islands grown on Si (001) were dome-shaped while the islands on Si (113) were multiple-side faceted with flat tops of (113)-orientation. In contrast, for Ge deposition with a lower growth rate of ${\sim}0.15\;ML/min$ on Si(113), we observed the shape transition from circular into elongated island structures. The elongated islands grew longer along the [$33\bar{2}$] during continuous Ge deposition. The shape evolution of the islands is discussed in terms of strain relaxation and kinetic effects.

Assessment of the Residues of Benfuresate and Oxolinic Acid in Crops (Benfuresate와 Oxolinic Acid의 작물체중 잔류량 평가)

  • Park, Dong-Sik;Yang, Jae-E.;Han, Dae-Sung
    • Korean Journal of Environmental Agriculture
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    • v.14 no.3
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    • pp.312-318
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    • 1995
  • Residues of benfuresate in rice and oxolinic acid in Chinese cabbage were determined through the field experiments in order to establish the safe use and the maximum residue limit(MRL) of these pesticides in Korea. The herbicide benfuresate powder was sprayed into the paddy field with a level of 0.6kg(active ingredient)/ha and rice (Oryza sativa L.) was grown. At harvest, residues of benfuresate in brown rice and stem were analyzed using gas chromatograph. The residue of oxolinic acid in Chinese cabbage (Brassica campestris subsp. napus var pekinesis MAKINO) was analyzed using HPLC after foliar-spraying this fungicide into the cabbages at a level of 15kg/ha. The recovery efficiencies of benfuresate and oxolinic acid were 87-89% and 90-95%, respectively. The respective residues of benfuresate in rice and oxolinic acid in Chinese cabbage were in the range of 0.27-0.46 mg/kg and 0.23-1.53kg/kg. Residual concentrations of these pesticides in crops increased with the increased application frequencies, followed the first-order kinetics and linearly decreased with time. The highest residue of 1.53 mg/kg of oxolinic acid was observed when this fungicide was sprayed six times until three days prior to harvest, but this level was far lower t㏊n 5 mg/kg, which is the maximum residue limit(MRL) set by FAO/WHO.

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Leaching and Adsorption of Flupyrazofos(KH-502) in the Soil (Flupyrazofos(KH-502)의 토양 중 용탈 및 흡착)

  • Yang, Jae-E;Cho, Boo-Yeon;You, Kyoung-Youl
    • Korean Journal of Environmental Agriculture
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    • v.16 no.1
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    • pp.72-79
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    • 1997
  • Adsorption, leaching, and retention of the Flupyrazofos(KH-502), a new active ingredient for insecticide, in the soils under laborarory and field conditions were investigated to provide the basic data for the safety use and to assess a secondary impact of this insecticide on soil and water environments. A significant power function relation was found between the adsorbed KH-502 and time, representing that 45% of the added KH-502 was adsorbed within 30 min. but a quasiequilibrium was reached after 6 to 12 hr with a slower adsorption. Adsorption phenomena followed th first-order kinetics and time required for 50% adsorption was 5.8 hr. The equilibrium adsorption isotherm was explained by the Freundlich equation and was classified as S-type. The amounts of KH-502 leached through the soil column (C) as compared to initial conc. ($C_0$) were very low and these relative concentrations ($C/C_0$) were 0.073 and 0.017 in SL and CL soils, respectively. The residual conc. of KH-502 in the surface soil was comparatively low and decreased with time. Half-lives of KH-502 in the surface soil was comparatively low and decreased with time. Half-lives of KH-502 under the field conditions were estimated to be 20 and 18 days in the SL and CL soils, respectively. The KH-502 cone, transported to the subsurface soils was extremely low. These results demonstrate that KH-502 has a low pollution risk potential to the surrounding environment as far as it is used following the recommended guideline.

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Mineralogical Study of the Granite Weathering in the Seoul Area: Water-Rock Interaction in the Namsan Granite (남산 화강암의 풍화 및 광물-물 반응에 관한 연구)

  • Lee, Soo-Jae;Kim, Soo-Jin
    • Journal of the Mineralogical Society of Korea
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    • v.7 no.1
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    • pp.40-48
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    • 1994
  • The weathering of the Namsan granite was studied in terms of sorption process. The Namsan granite consists mainly of quartz, alkali feldspar, plagioclase (${Ab_{85}An_{15}}-Ab_{100}$ and biotite with small amounts of sericite, magnetite and ilmenite. The kinetic factors for altering the granite body are the proton and hydroxyl ions derived from the reaction of water and mineral. There are two different types of pH variation curves for rocks of different mineral assemblages. when powdered granite was dispersed in distilled water under ambient condition. The sorption-process proceeds by three steps for fresh granite; (1) the initial rapid pH-rise to 10 by the uptake of proton by negatively charged mineral surfaces, (2) the gradual pH-down, and (3) the stable pH tail between 7.1-7.5. For somewhat weathered granites, the sorption proceeds; (1) the initial rapid pH-down to 4.8, (2) the slight pH-rise and slow ph-down, and (3) the stable pH tail between 5.0-5.3. The reaction rate is controlled by the density of adsorbable sites, the solubility of the mineral, pH of the system and formation of amorphous gel and gibbsite. Amorphous gel floates on the surface of the solution while stirring the powdered granite and then is transformed into gibbsite in an hour or so. The pH saturation values for -325 mesh fresh granite from 5 m depth is about pH 10 when rock/water ratio is over 10g/200 ml.

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A Kinetic Study of GaN Formation from GaOOH under $NH_3$ Flowing ($NH_3$ 분위기에서 GaOOH로부터 GaN의 반응기구)

  • 이재범;이종원;박인용;김선태
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2003.03a
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    • pp.94-94
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    • 2003
  • 최근, 새로운 전자재료로서 GaN 분말의 합성과 응용에 관한 연구가 많이 이루어지고 있다. CaN 분말은 열처리 과정 중 분해를 방지하기 위한 표면 보호용 소재, CaN 박막 또는 벌크 결정을 성장하기 위한 precursor 및 대면적 평판표시소자 제작을 위한 전기발광소자용 소재 등에 적용되고 있다. 일반적으로 100$0^{\circ}C$ 이상의 온도에서Ga과 NH$_3$를 반응시키거나, Ga이 포함된 화합물반도체 또는 산화물 및 질산염 등을 NH$_3$ 분위기에서 가열시켜 GaN 분말을 합성시키고 있다. 본 논문에서는 출발물질로서 GaOOH를 채택하고, 이를 NH$_3$ 가스를 흘리면서 가열 반응시켜 GaN 분말을 합성하고 X선 회절분석 방법을 사용하여 GaN의 합성에 대한 반응기구를 조사하였다. GaN 분말을 합성하기 위하여 GaOOH 분말 1g을 석영 용기에 담아 석영 반응관 내에 위치시키고, 반응관 내부를 $10^{-3}$ torr의 진공으로 배기한 후 $N_2$를 주입하면서 전기로의 온도를 1$0^{\circ}C$/min으로 승온시켰다. 반응온도는 300~l17$0^{\circ}C$의 범위에서 변화시켰고, 반응시간은 10분부터 24시간까지 변화시켰으며, NH$_3$의 유량은 300~700 sccm의 범위에서 변화시켰다. GaN의 반응역학을 조사하기 위하여 X선 회절도에서 특정 성분의 회절강도는 시료 내에 포함된 특정 성분의 량에 직접 비례한다고 가정하고, 2$\theta$=37$^{\circ}$부근에서 관찰되는 GaN의 (101)면에 의한 회절강도를 측정하고, 이를 GaN의 생성량으로 고려하였다.}C$로 소결 하였다. coating 결과 박리현상은 없었으나, 표면과 단면의 SEM분석결과 다소 porous한 박막층이 형성되었으며, Ca이온이 지지체로 permeation되는 현상이 발생하였다. 이와 같은 결과로부터 보다 치밀한 박막생성을 위해, slurry 제조조건을 변화시켰으며, Ca이온의 migration을 막기 위해 barrier layer를 이용하였다 완전 소결된 지지체는 가스투과도와 전기전도도측정을 통하여 특성을 평가하였다.였다.다.m이하의 NH$_3$ 가스를 검출할 수 있었다.기 화강암 관입 이전에 좌수향 전단 운동에 의해 부분적으로 재활성 되었으며, 후기 화강암의 관입 이후에 재차 우수향 전단운동으로 활성화 되었음을 알 수 있다. 이상의 결과를 종합하면 호남전단대는 쥬라기 중기에 발생한 광역적인 우수향의 연성전단운동이나, 운동 특성은 연속적이기 보다는 단속적으로 일어난 것으로 생각된다.리 폐 관류는 정맥주입 방법에 비해 고농도의 cisplatin 투여로 인한 다른 장기에서의 농도 증가 없이 폐 조직에 약 50배 정도의 고농도 cisplatin을 투여할 수 있었으며, 또한 분리 폐 관류 시 cisplatin에 의한 직접적 폐 독성은 발견되지 않았다이 낮았으나 통계학적 의의는 없었다[10.0%(4/40) : 8.2%(20/244), p>0.05]. 결론: 비디오흉강경술에서 재발을 낮추기 위해 수술시 폐야 전체를 관찰하여 존재하는 폐기포를 놓치지 않는 것이 중요하며, 폐기포를 확인하지 못한 경우와 이차성 자연기흉에 대해서는 흉막유착술에 더 세심한 주의가 필요하다는 것을 확인하였다. 비디오흉강경수술은 통증이 적고, 입원기간이 짧고, 사회로의 복귀가 빠르며, 고위험군에 적용할 수 있고, 무엇보다도 미용상의 이점이 크다는 면에서 자연기흉에 대해 유용한 치료방법임에는 틀림이 없으나 개흉술에 비

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The Relationship between health related physical fitness and self-perceived health status (건강관련 체력과 주관적 건강인식에 관한 연구)

  • Kim, Jae-Hoon;Jin, Young-Soo;Park, Jung-Tae;Jee, Yong-Suk;Kim, Kun-Soo;Lee, Heun;Bae, Ki-Taec
    • Korean Journal of Health Education and Promotion
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    • v.16 no.1
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    • pp.83-100
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    • 1999
  • Modern technology has lessened the physical demand of every activities. Thus, physical inactivity has led to a rise in ‘Hypokinetic diseases’. The prefix, hypo, means lack of and kinetic refers to movement. Individuals who do not exercise regularly are at greater risk of developing hypokinetic diseases such as coronary heart disease, hypertension, hyperlipidemia, obesity, musculoskeletal disease. Systematic exercise program defenses development of hypokinetic diseases. Exercise program bases on basic physical fitness test. The physical fitness evaluation is used to degree of Wellness of the individuals. It includes the evaluation of Health-Related Physical Fitness -musclular strength, muscular endurance, flexibility, cardiorespiratory endurance, body composition - and skill or sports related fitness -agility, balance, coordination, reaction time, speed. In present study, the authors investigated health-related physical fitness test result and questionnaire for 133 male and 71 female from Jannuary 16 to March 15, 1997. The purpose of this study is to observe relationship health related physical fitness and self-perceived health status. The results are as follows. 1. There were statistically significant differences in BMI, grip strength, back strength, muscular endurance, cardiorespiratory endurance, and flexibility between male and female subjects. Flexibility is higher in female than male subject, but Others are higher in male than female subjects. 2. There were statistically significant differences in grip strength, muscular endurance between 30s, 40s, and 50s group In both gender subjects, and In cardiorespiratoryendurance between the groups especially in Female subjects. 3. For male subjects, flexibilitywas measured lower in group who classified low level in self-perceived health status than group of others. Also, for female, subjects, Muscular endurance & flexibility were measured. 4. It was shown that grip strength, back strength, and muscular endurance were significantly lower in group who have chronic diseases than normal group for Male subjects. But, For female subjects, all the component were statistically insignificant results between normal and disease group. 5. Both male and female subjects, there were Positive correlation among grip strength, back strength, muscular endurance, cardiorespiratory endurance, and flexibility but, negative correlation for age. Therefore, health-related physical fitness is very important component for Heath Promotion & Wellness. Physical Fitness test is valuable test in health evaluation, health management and health promotion, so available for hospital, sports center, community health center, industrial field, school etc.

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Properties of Foamed Concrete According to Types and Concentrations of Foam Agent (기포제 종류 및 희석 농도에 따른 기포 콘크리트의 특성)

  • Kim, Jin-Man;Jeong, Ji-Yong;Hwang, Eui-Hwan;Shin, Sang-Chul
    • Journal of the Korea Concrete Institute
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    • v.24 no.1
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    • pp.61-70
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    • 2012
  • Recently, the government has been working feverously to save energy and reduce greenhouse gas emission by enacting Basic Act on Low Carbon Green Growth at the national level. Improving the insulation performance of building exterior and insulator can reduce the energy in the building sector. This study is about developing light-weight foamed concrete insulation panel that can be applied to buildings to save energy and to find the optimal condition for the development of insulation materials that can save energy by enhancing its physical, kinetic and thermal characteristics. Various experimental factors and conditions were considered in the study such as foam agent types (AES=Alcohol Ethoxy Sulfate, AOS=Alpha-Olefin Sulfonate, VS=Vegetable Soap, FP=Fe-Protein), foam agent dilution concentration (1, 3, 5%), and foam percentage (30, 50, 70%). Experiment results indicated that the surface tension of aqueous solution including foam agent, was lower when AOS was used over other foam agents. FP produced relatively stable foams in 3% or more, which produced unstable foams containing high water content and low surface tension when diluted at low concentration. Depending on foam agent types, compressive strength and thermal conductivity were similar at low density range but showed some differences at high concentration range. In addition, when concentrations of foam agent and foaming ratio increased, pore size increased and open pores are formed. In all types of foam agent, thermal conductivity were excellent, satisfying KS standards. The most outstanding performance for insulation panel was obtained when FP 3% was used.