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Lactic Acid Fermentation of Onion Juice Supplemented with Puffed Red Ginseng Extract (팽화 홍삼 추출액을 첨가한 양파 착즙액의 유산 발효)

  • Park, Young-Seo;Jang, Jae Kweon;Choi, Young Jin;Chung, Myong-Soo;Park, Hoon;Shim, Kun-Sub
    • Food Engineering Progress
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    • v.13 no.1
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    • pp.16-23
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    • 2009
  • Onion juices supplemented with different concentrations of puffed red ginseng extract were fermented using Pediococcus pentosaceus KC-007 and their biologically functional properties were investigated. When onion juices were supplemented with puffed red ginseng extract at the concentration of 0.5, 1, 2, and 4% (v/v) each, viable cell number of lactic acid bacteria was the highest at 24 hr of fermentation in all samples. The titratable acidity increased as the fermentation proceeds irrespective of the added amount of red ginseng extract, and the pH of fermentation broth decreased until 36 hr of fermentation. The reducing sugar of fermentation broth decreased until 24 hr of fermentation and did not change thereafter. The electron donating ability and nitrite scavenging ability were highest when red ginseng extract was added at the concentration of more than 1% (w/v). The overall acceptance in sensory evaluation was the best when red ginseng extract was added at the concentration of 1% (w/v). From these results, it is confirmed that the optimum concentration of puffed red ginseng extract for the lactic acid fermentation of onion juice was 1% (w/v).

Prediction of the Scour Depth around the Pipeline Exposed to Waves using Neural Networks (신경망을 이용한 파랑하 관로주변의 세굴심 예측)

  • Kim, Kyoungho;Cho, Junyoung;Lee, Hojin;Oh, Hyunsik
    • Journal of the Korean GEO-environmental Society
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    • v.14 no.5
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    • pp.15-22
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    • 2013
  • The submarine pipe, which is one of the most important coastal structures, is widely used in the development of coastal and ocean engineering. The scour of the submarine pipe occurs due to the wave and the current according to the state of the sea bed. The scour affects the submarine pipe and causes it to undergo settlement and fatigue. It is difficult to predict the local scour under complicated and various conditions of the coastal environment, even though many researches on the scour of the submarine pipe have been studied in recent years. This study analyzed the scour depth around a submarine pipe by using the Neural Network technique. The back-propagation algorithms was used to train the Neural Network. The 58 simulating experimental data for the performance and validation of the Neural Network technique were analyzed in this study. Then, the regression analysis for the same data was performed in this study to predict and compare with the Neural Network technique for the scour depth.

Studies on the Consumptive Use of Irrigated Water in Paddy Rice (수도 품종간의 필요수량 차이에 관한 연구)

  • 김시원;오완석;김선주
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.23 no.2
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    • pp.35-44
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    • 1981
  • This experiment was conducted to find out the consumptive use of irrigated water for calculation of duty water in paddy rice. Tall statured Japonica rice varieties, Nongbaek (early), Jinheung (medium) and Akibare (late), and short statured Tongil typed varieties, Josaeng Tongil(early), Suweon 264 (medium) and Suweon 258(late) were planted on the experimental farm of Kon-Kuk University in 1979. The results obtained in this study were as follows: 1. During the experimental period, the daily mean temperature was almost similar, the relative humidity was higher as much as 2.8%, the amount of rain fall was 100mm less and the pan evaporation was 70mm less compared with those of 30 years average, respectively. 2. The paddy soil was silty loam, which was suitable for the rice cultivation. 3. Varietal differences were find out for plant height, culm length, number of tillers, number of panicles, heading date, matured grain ratio, 1000-grain weight and rough rice yield. This difference might he the cause of varietal difference of the consumptive use of irrigated water during the rice growing period. 4. The evapotranspiration was gradually increased after transplanting and showed the peak from booting to heading stage of rice varieties. The average evapotranspiration through the whole growing period was 5.67-5. 80mm/day for tall statured Japonica varieties, and 5.99-6. 39mm/day for short statured Tongil typed varieties. 5. The ratio of evapotranspiration to pan-evaporation through the whole growing period was 1.49-1.50 for Japonica varieties, and 1.60-1.66 for Tongil typed varies. 6. Average amount of percolation in paddy field was 3. 62mm/day through the whole growing period of rice plant. 7. K-value in Blaney & Criddle formula was 0.94-0.98 for Japonica varieties and 1.02-1.08 for Tongil typed varieties, and coefficient consumptive water use (Kc-value) was 0.95-1.02 for Japonica varieties and 1.04-1.12 for Tongil typed varieties in this study. The modified coefficient for consumptive water use, which was calculated from data collected through the country including this study, was as follows;

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Elementary Pre-service Teachers' Mathematical Knowledge for Teaching (MKT) on Number and Operations (수와 연산 영역에 대한 초등 예비 교사들의 수학을 가르치는데 필요한 지식(MKT))

  • Kim, Hae-Gyu
    • Communications of Mathematical Education
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    • v.26 no.1
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    • pp.71-84
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    • 2012
  • The purpose of this study is to analyze some Korean elementary pre-service teachers' Mathematical Knowledge for Teaching(MKT) and compare the results with those obtained by Kwon, Nam, & Kim(2009), so that we can provide some suggestions to improve education of elementary mathematics subject at Korean teachers colleges. For this purpose, we selected the MKT items on number and operations which were adapted for Korean in-service teachers by Kwon et al. The survey consisting of those items was administered to 88 Korean elementary pre-service teachers at teachers college, J University. The results are the following: First, the respondents, elementary pre-service teachers, showed that they already had a sufficient amount of Content Knowledge(CK) on number and operations, but that their level of Knowledge of Content and Students(KCS) was insufficient. This means we need to strengthen our students' KCS in education of elementary mathematics subject at our teachers colleges. Second, there was a strong correlation, in both CK and KCS, of item difficulty felt by the respondents with that by the Kwon et al's in-service teachers. Third, although the respondents valued the MKT items more than the above-mentioned elementary in-service teachers, about 70% of them said the items were never learned at their college. Furthermore, they had different opinions on some of the items from their counterparts'. The suggestions we get here are we need to first consider the results in improving education of elementary mathematics subject at our teachers colleges and second develop MKT items suitable for the situation of Korean schools and curriculums in order to obtain exact estimations of Korean elementary pre-service teachers' MKT.

A Web-based Microcontroller Remote Laboratory for Sharing Resources (자원공유를 위한 웹기반 마이크로콘트롤러 원격 실험실)

  • Moon, Il-Hyeon;Han, Sae-Ron;Cho, Kwan-Sun;Ahn, Dal;Lim, Jong-Sik;Jeon, Heung-Gu
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.9 no.5
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    • pp.1184-1192
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    • 2008
  • There have been a large number of trials of the experimental education in engineering using virtual laboratories implemented in virtual space, namely on the Web. Although virtual education is regarded as an effective educational means that overcomes the limit of time and space and bring high teaming effects, the problem of lack of reality must be solved. The present study proposed and implemented a real-time remote laboratory as a solution for the problem of lack of reality in virtual education. The remote laboratory provides functions with which learners can perform experiments by remote-controlling circuits and equipment related to experiments and practices on the Web and gets data and image information, so through it learners can make realistic experiments at any time and in any place. The remote laboratory module based on Internet open sources such as JAVA Web start technology, FTP technology and Telnet technology in server-client environment can be implemented at low cost, and is effectively applicable to engineering experiment education in various areas related to real-time hardware control.

Isolation and Characterization of the Indigenous Microalgae Chlamydomonas reinhardtii K01 as a Potential Resource for Lipid Production and Genetic Modification (지질생산 및 유전자 조작의 잠재적 자원으로서의 토착 미세조류 Chlamydomonas reinhardtii K01의 분리 및 특성)

  • Kim, Eun-Kyung;Cho, Dae Hyun;Suh, Sang-Ik;Lee, Chang-Jun;Kim, Hee-Sik;Suh, Hyun-Hyo
    • Journal of Life Science
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    • v.32 no.3
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    • pp.202-209
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    • 2022
  • The green alga Chlamydomonas reinhardtii, a unicellular haploid eukaryote, has long been used by researchers and industries as a cell factory to produce high value-added microalgae substances using genetic modification. Microalga K01, presumed to be Chlamydomonas, was isolated from 12 freshwater samples from the Chungcheong and Jeolla regions to replace C. reinhardtii, an introduced species currently used in most basic and industrial research. The isolated K01 strain was identified as C. reinhardtii through morphological and phylogenetic studies of the 18S rDNA gene sequence (NCBI accession number KC166137). The growth and lipid content of the isolated C. reinhardtii K01 were compared with three wild and four mutant strains in TAP medium, and it was found that the K01 strain could produce 1.74×107 cells/ml by the third day of culture. The growth rate of C. reinhardtii K01 was 1.5 times faster than UTEX2244, which showed the highest number of cells (1.20×107 cells/ml) among the compared strains. The lipid content of the isolated C. reinhardtii K01 (20.67%) was similar to those of the wild strains, although the fatty acid oleate C18:1 was not detected in the isolated strain but was identified in the seven others. The cell density of the isolated strain increased to 0.87 g/l during a six-day culture in BG11 medium, where nitrate (NaNO3) was introduced as a nitrogen source, while the seven acquired strains showed almost no cell proliferation.

A Study on the Effects of Married Women's Intention of Childbirth (기혼여성의 출산의향에 영향을 미치는 요인에 관한 연구)

  • Un, Sun-Kyoung;Park, Hyo-Jin
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.4
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    • pp.210-217
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    • 2020
  • This paper analyzes the factors influencing the childbirth intention of married women with the goal of analyzing whether times of spouses' housework, marital satisfaction, values of children and traditional gender role, and social services related to the reduction of low fertility contribute to women's intention to give birth. The respondents of the research were 1,000 married women, and the research was conducted with 482 women of childbirth intention. The findings are as follows. Age of sociodemographic factors has an influence on the childbirth intention. Related to family's character, factors influencing childbirth intention are number of children, times of spouses' housework, and marital satisfaction. In addition, emotional and instrumental values of children, number of recognized public services for reduction of low fertility. In other words, the more women share housework with spouses and satisfy their marital life, the more childbirth intention of women is increased. In order to improve childbirth intention, changes of perception of gender role and sharing housework, reinforcement of social system for work and family compatibility that convert changes of perception into action, advanced marital relationship programs, extensive support for subfertility and a huge build-up of social services related to reduction of low fertility need to be provided.

A Study on the Consumptive use of Irrigated Water in Paddy Rice (벼의 품종별(品種別) 필요수량(必要水量) 관(關)한 실험분석(實驗分析))

  • Suh, Seung Duk;Choi, Won Chil
    • Current Research on Agriculture and Life Sciences
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    • v.3
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    • pp.36-43
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    • 1985
  • This study was conducted to find out the consumptive use of irrigated water for calculation of a duty of water in paddy rice. Tall statured Japonica rice variety is Jinju(late). Short statured Tongil varities-Taebaek(early) and Pungsan(medium) were planted on the experimental farm of kyungpook National University in 1983. The results obtained in this study were as follows : 1. The average temperature of the underwater surface was $27.1^{\circ}C$ and the maximum was recorded at beginning of the August. 2. The evapotranspiration was gradually increased after transplanting and showed the peak from booting to heading stage of rice varieties. The average evapotranspiration through the whole growing period was 8. 43mm/day(Jinju) for tall statured Japonica variety, and 7.78mm/day(Taebaek), 7.71mm/day(Pungsan) for short statured Tongil varieties. 3. The ratio of evapotranspiration to pan-evaporation through the whole growing period was 1.71 for Japonica variety and 1.55 for Tongil varieties. 4. K-value in Blaney & Criddle formula was 1.41 for Japonica variety and 1.30 for Tongil varieties. Coefficient consumptive use of water (Kc-value) was 1.40 for Japonica variety and 1.27 for Tongil varieties. 5. It appeared that the water consumption in the 6, 8 cm pot is more than that of 2, 4 cm pot but the number of panicle of the 6, 8 cm pot is somewhat less than that of 2, 4 cm pot. The deeper water level is the less tiller became. It is consindered that 2 cm pot is more profitable than the others.

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Effect of Recombinant CagL Immunization on the Gastric Diseases Induced by Helicobacter pylori in Mongolian gerbils (CagL 재조합 단백질 접종후에 Mongolian gerbil에서 나타나는 Helicobacter pylori 감염에 대한 반응)

  • Bak, Eun-Jung;Jang, Sung-Il;Choi, Yun-Hui;Kim, Jin-Moon;Kim, Ae-Ryun;Kim, Ji-Hye;Woo, Gye-Hyeong;Yoo, Yun-Jung;Lee, Sung-Haeng;Cha, Jeong-Heon
    • Korean Journal of Microbiology
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    • v.48 no.2
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    • pp.109-115
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    • 2012
  • Helicobacter pylori is an important factor of chronic gastritis, digestive ulcer, and stomach cancer. CagL, a virulence factor of H. pylori, is well-known as a pilus protein which acts as adhesion to host cell and a component of Type 4 secretion system. In this study, we evaluated the protective response of recombinant CagL protein (rCagL) using Mongolian gerbil animal model for H. pylori infection. The cagL gene was cloned from 26695 H. pylori followed by over-expression and purification of the protein in E. coli. Mongolian gerbils were immunized with rCagL protein mixed with aluminum adjuvant via intramuscular injections once a week during 4 weeks. At a week after the last immunization, the Mongolian gerbils were administrated with H. pylori 7.13 strain into the stomach and sacrificed to measure antibody titer on rCagL by ELISA and bacterial colonization in the stomach, and to examine the histopathological changes and cytokine expression at 6 week after challenge. Antibody titers on recombinant protein were significantly increased from a week after the first immunization. There was no significant change of the number of bacterial colony between control group and immunized group. The relative stomach weight was significantly decreased in immunized group, but the significant change of histopathological assessment was not observed in the stomach. Cytokine expression such as IL-$1{\beta}$ and KC also was not significantly different between control and immunized groups. These results indicate that rCagL could effectively induce the formation of the specific IgG antibodies. However, bacterial colonization and histopathological lesions could not be inhibited by the immunization in the stomach, indicating not enough protection against H. pylori infection. We consider that along with CagL other adequate antigens could be needed stimulating immune response and inducing protective effects against gastric disease, and also a better adjuvant could be considered.

Strain Improvement of Penicillium verruculosum for High Cellulase Production by Induced Mutation (섬유소분해효소 생산증진을 위한 Penicillium verruculosum의 균주개량)

  • 정기철
    • Microbiology and Biotechnology Letters
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    • v.15 no.6
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    • pp.388-395
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    • 1987
  • In order to obtain a regulatory mutant strain with high cellulase activity, a newly isolated Penicillium verrculosum, strain F-3 was used as parental strain since it was proved to be an efficient cellulase producer. A number of experiments were conducted to determine the optimum conditions to in-duce mutagenesis and isolate the desirable mutant strains. Out of several restriction compounds tested, 1.5% oxgall was found to be most effective to restrict the colony size by suppressing overgrowth. Derepression of catabolites was employed as a criterion in selecting mutant strains with high cellulase productivity. Production of cellulase by Penicillium venculosum F-3 was suppressed when cultured on the media with more than 1% of glucose or glycerol. It was found that either irradiation with UV light for 19 mins or treatment with nitrosoguanidine at 200$\mu\textrm{g}$/m1 for 60 mins, induced mutagenesis at desired level, when the survival rate of the spore was 0.2% and 48%, respectively. Three mutant strains of F-3, UV-9, UV-10, and NTG-3 that had the highest cellulase productivity were finally selected, based on filter paper degradation rate, size of clearing zone on the screening plate and cellulase activity in the medium containing cellulose powder. When the mutant strains were compared with parental strain F-3, on the KC-M-W medium containing cellulose powder, the filter paper activities of UV-9, UV-10, and NTG-3 were increased by 34%, 55%, and 41%, respectively. However, the assimilation of cellobiose octaacetate by UV-9 or NTG-3 was markedly reduced. When the mutant UV-10 was grown on cellobiose octaacetate medium (CCA-4) in shaking flasks, the cellulase activities of the mutant increased by 20 to 50% compared to the parental strain. Excreation of soluble protein from the mutant also elevated up to 30%. The mutant also constitutively produced both CMCase and $\beta$-glucosidase, though at relatively low level, in the presence of glucose or cellobiose as carbon sources.

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