• Journal of Microbiology and Biotechnology
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• v.19 no.4
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• pp.351-357
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• 2009

Natural wild-type strains of Bacillus subtilis are extensively used in agriculture as biocontrol agents for plants. This study examined two antagonist B. subtilis strains, KB-1111 and KB-1122, and the results illustrated that KB-1122 was a more potent inhibitor of the indicator pathogen than KB-1111. Thus, to investigate the intrinsic differences between the two antagonist strains under normal culture conditions, samples of KB-1111 and KB-1122 were analyzed using MALDI-TOF-MS. The main differences were related to 20 abundant intracellular and 17 extracellular proteins. When searching the NCBI database, a number of the differentially expressed proteins were identified, including 11 cellular proteins and 10 secretory proteins. Among these proteins, class III stress-response-related ATPase, aconitate hydratase, alpha-amylase precursor, and a secretory protein, endo-l, 4-beta-glucanase, were differentially expressed by the two strains. These results are useful to comprehend the intrinsic differences between the antagonism of KB-1111 and KB-1122.

• Microbiology and Biotechnology Letters
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• v.18 no.6
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• pp.647-652
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• 1990

The production of delta-endotoxin crystal and the cloning of endotoxin protein gene in Bscillus thuringiensis subsp. kurstaki HD1 strain were studied. The strain produced bipyramidal crystals ($2.9\times 1.0 \mu m$) in their cells during sporulation. The B. thuringiensis contained about 10 plasmid DNA elements ranging from 2.1 to 80 kilobases. The 73 kb plasmid DNA, the 29 kb BamHI fragment and the 7.9 kb Pstl DNA fragment hybridized to the pHL probe. The 7.9 kb fragment was eluted and cloned in the PstI site of pBR322 vector and transformed into E. coli HB101, which produced insecticidal proteins killing Bornbyx mori larvae.

• Korean Journal of Veterinary Service
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• v.23 no.1
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• pp.77-91
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• 2000

This study was conducted to investigate the antibiotic resistance and plasmid profiles of 58 salmonella spp isolated from mesenteric lymphnodes of slaughter pigs in Kyoungbuk province during the period from September 1997 to June 1998. The results obtained are as follow that all isolates were susceptible to amikacin, gentamicin, ciprofloxacin and enrofloxacin, and the majority of isolates were highly susceptible to norfloxacin, colistin, nalidixic acid and apramycin while they were moderately susceptible to kanamycin, cephalothin, chloramphenicol, ampicillin, trimethoprim and penicillin. The majority of isolates were over 90% resistant rates to lincomycin, sulfadimethoxine, vancomycin, methicillin and erythromycin. The plasmid profiles of 58 salmonella spp are developed 1 to 4 fractions, 0.9 to 29.5 Kb molecular range sizes and U strains (45.5%) were showed plasmid profiles by agarose gel electrophoresis. 5 derby harbored 29.5 Kb and 7 Kb, and S schwarzengrund had 14 Kb and 0.9 Kb harboring sizes. Four of 10 S agona and 2 of 4 S typhimurium were harbored 3.1 Kb and n.5 Kb, respectively. Thirty-five untypable strains are developed variable size fractions its showed small size plasmid profile less than 6 Kb and 22 (62.8%) of them had no detectable plasmids.

• Korean Journal of Organic Agriculture
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• v.8 no.3
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• pp.121-130
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• 2000

In order to screen the antagonistic bacteria which inhibit the growth of the plant pathogen, Penicillum expansum, we isolated an effective bacterial strain and investigated into the antifungal activity of the antagonist and it's identification. The eleven strains of bacteria which strongly inhibited P. expansum were isolated from the nature, and the best antagonistic bacterial strain designated as KB22, was selected. The antagonistic strain KB22 was identified to be the genus Bacillus subtilis based on morphological and biochemical characterization, The KB22 showed 55.9% of antifungal activity against the growth of P. erpansum. By the treatment of the culture broth and the heat treated culture filtrate of it, the B. subtilis KB22 showed 90% and 15% of antifungal activity, respectively.

• Journal of the Korean Society of Tobacco Science
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• v.14 no.2
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• pp.97-103
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• 1992

KB 101 is a bacterial wilt(Pseudomonas solanaceamm E.F. Smith) and black shank (Phytophthora nicotianae Breda de Haan Var. nicotianae Waterhouse) resistant cultivar of burley tobacco (Nicotiana tabacum L.) KB 101 was developed by the Korea Ginseng&Tobacco Research Institute, and released in 1987. KB 101 was developed from a single plant selection in the F2 generation derived from the double cross, [(Burley 21X Burley 37) X (Burley 64X Ky 16)]. Burley 37 and Burley 64 were the source of resistance to bacterial wilt and black shank. Yield trials were conducted in the Fs through F6 generations at the four Exp. Stn. of Korea Ginseng &Tobacco Research Institute as JB 7705-1. On-farm yield trials were conducted in the F7 through F9 generations at the 45 locations of burley tobacco growing area from 1984 to 1986 as KB 101. KB 101 has an erect growth habit similar to that of Burley 21: plant size is larger and has more leaves than those of Burley 21. It is late maturing cultivar that flowers approximately 3 days later than Burley 21. The physical characteristics and chemical composition of KB 101 were similar to those of Burley 21.

• Asia Marketing Journal
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• v.14 no.3
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• pp.153-167
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• 2012

KB Kookmin Card has separated as an independent corporation from KB Kookmin Bank Credit Card Business Group on March, 2011. Ever since, KB Kookmin Card worked to build its own brand identity. The strategic preparation and conscientious implementations made KB Kookmin Card position in consumer's mind with a strong and unique brand image. Its new brand image was rooted in the inherited strengths of reliable and sincere image. However, it faced the challenge to compete in credit card industry in which most competitors had an advanced and sophisticated image. The strengths of KB Kookmin Card were also at the same time their limitations. KB Kookmin Card took a strategy that strengthened the strengths and improved the weaknesses. It focused on the core competence of being a people's sincere life supporter that helps people make savings from everyday events to make a good sum rather than being a lump sum benefit. The brand introduction strategy was implemented in 2011. The implementation focused on the activities that made internal as well as external customers be aware of the brand. Communication programs using a variety of media were executed to attain this goal. In 2012, second phase communication programs were introduced to elaborate the newly established brand image. It introduced many extended products as well as accessory programs which targeted the segments. Also, various CSR activities in many social domains helped consumers and the public to consider KB Kookmin Card an authentic, caring, trustworthy, and consistently-developing supporter in their everyday lives.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.70-70
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• 2002

형질전환 가축을 생산하기 위하여 최근 체세포 복제 기법을 이용하고 있다. 이러한 체세포를 이용한 형질전환 동물의 생산에는 체세포내에 유전자의 도입 효율이 직접적인 영향을 주게 된다. 따라서 본 연구는 세포내 유전자의 transfection 효율을 높이고자 한우의 체세포를 이용하여 여러 가지 조건에서 유전자 도입을 실시하였다. 세포내 유전자 도입 방법은 electroporation (EP) 방법을 이용하였다. 사용한 세포는 소의 귀세포(KbESF), 태아섬유아세포 (KbFF), 그리고 대조구로서 CHO cell을 이용하여 GFP 유전자를 도입하였다. EP는 0.4 cm cuvette을 사용하였고, voltage는 0.25 kV, 그리고 field strength 는 0.625 kV/cm 조건으로 실시하였으며, pulse times은 각각 1, 2, 또는 3회를 사용하였다. KbFF와 KbESF에서는 각각 pulse times을 증가시킬수록 유전자도입 세포수가 증가하였으나 (KbFF: 81, 634, 1,065 cells/$10^{6}$ cells, KbESF: 1,011, 5,567, 15,408 cells/$10^{6}$ cells), CHO cell에서는 pulse times을 증가시킬 수록 오히려 유전자도입 세포수가 감소하였다 (CHO: 1,591, 687, 297 cells/$10^{6}$ cells). 그리고 2주 동안 neo selection을 실시 한 결과 KbFF, KbESF, CHO에서 각각 93, 35, 184 colony가 선발되었으며, 이 중 65.6%, 8.6%, 4.3% 가 GFP 형광 발현 colony로 나타났다. 한편 CHO cell에서 transfection cell수가 감소된 것은 EP의 자극으로 인해 손상된 세포가 많이 발생한 것으로 나타났다. 또한 neo selection에서 선발된 colony중 GFP가 발현되지 않거나 일부만 발현되는 colony들이 많이 발생하였는데, 이것은 세포내 유전자가 transfection되지 않은 세포도 neo selection에서 선발된다는 것을 제시하고 있다. 따라서 체세포를 이용한 형질전환동물 생산을 위해서는 세포내 유전자 도입과 선발 과정에서 나타난 colony에 대하여 보다 엄격한 screen을 하는 것이 필요한 것으로 생각된다.

• The Korean Journal of Mycology
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• v.23 no.2 s.73
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• pp.190-195
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• 1995

For the screening of biocontrol agents against red-pepper anthracnose (Colletotrichum gloeosporioides Penz) 248 isolates of bacteria, 51 of fungi and 30 of yeasts were obtained from phyllospere of medicinal plants. Of isolated microorganisms, four bacterial isolates, KB6, KB12, KB13 and KB14 were highly antagonistic to C. gloeosporioides than the others through dual culture test on potato dextrose agar (PDA). Among the four bacterial isolates, culture filtrate of the isolate KB12 showed the highest inhibition of C. gloeosporioides on PDA. The culture filtrates of four isolates controlled anthracnose on the red fruits, but not on the green fruits. In the living bacterial cell test, high control effect was observed both on the red and the green fruits. In the biochemical test, all isolates were identified as Bacillus subtilis.

• Korean Journal of Microbiology
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• v.31 no.4
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• pp.292-299
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• 1993

Agrobacterium tumefaciens KU12 isolated from Korea is able to induce tumors on various plants and catabolize octopine as a sole carbon and nitrogen source. A, tumefaciens KU12 contains three plasmids. Their sizes are 45.5 kb. 240 kb. and > 240 kb. respectively. For the purpose of identification of octopine type Ti plasmid, avirulent A, tumefacients A136 is transformed with plasmids isolated from KU12 by direct transformation. Transformants containing Ti plasmid were grown on AB medium containing octopine as a sole nitrogen source. The isolated strain, named KU911, contains only 240 kb plasmid. As a result of induction of crown gall and Southern hybridization with other octopine Ti plasmid pTiAch5, 240 kb plasmid named pTiKU12 was Ti plasmid.

• The Journal of Korean Obstetrics and Gynecology
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• v.24 no.4
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• pp.10-19
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• 2011

Objectives: This study was performed to elucidate the effects of Kwibi-tang extract(KB) on dermal fibroblast. Methods: To demonstrate the effects of KB on dermal fibroblast, we used human dermal fibroblast(F6) and UVB light(30 $mJ/cm^2$) was used to damage to dermal fibroblast. we measured the nitrite production, LDH release in UVB-irradiated dermal fibroblast to elucidate the action-mechanism of KB. Also, we evaluated cell proliferation of dermal fibroblast and the amount of increased PICP, TIMP-1 in dermal fibroblast. Results: 1. KB decreased the cell proliferation of F6 dermal fibroblast in concentration of 50 ${\mu}g/ml$. 2. KB decreased the synthesis of PICP in concentration of 50 ${\mu}g/ml$. 3. KB decreased the synthesis of TIMP-1 in concentration of 50 ${\mu}g/ml$. 4. KB have no effect on the damage in UVB-irradiated F6 dermal fibroblast. Conclusions: From the results, we concluded KB decreases the cell proliferation and collagen synthesis in dermal fibroblast. So we suggest that KB has the anti-hyperplasy of dermal fibroblast.