• Title/Summary/Keyword: KAP3

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Electron Microscopic Study on Extrachromosomal DNA from Splenocytes and Erythrocytes of Carassius carassius L. (붕어의 Splenocyte 및 Erythrocyte의 Extrachromosomal DNA 관찰)

  • Im, Sook-Ja;Kim, Woo-Kap
    • Applied Microscopy
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    • v.18 no.2
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    • pp.167-176
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    • 1988
  • Extrachromosomal circular DNA complexes from erythrocytes and splenocytes isolated from Carassius carassius were examined by mica-press-absorption method. The method was described that released small polydisperse circular DNA molecules in situ from the erythrocytes and the splenocytes and that allows selective observation of the small circular DNA complexes bound to cellular components. The released polydisperse circular DNA complexes were absorbed preferentially on mica in a divalent cation-free medium then processed for electron microscopy. Small circular DNAs showed a heterogeneous size distribution of $2{\sim}10{\mu}m$ with a mean contour length of $4.3{\mu}m$ for the circulating erythrocytes and that of $0.7{\sim}3.6{\mu}m$ with a mean contour of length $2.04{\mu}m$ for the splencytes. Cells contained $100{\sim}300$ copies and $300{\sim}700$ copies obtained from the erythrocytes and the splenocytes, repectively. Possible biological functional implications for size distribution of extrachromosomal circular DNAs are discussed.

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A Case Report of Tetanus Patient Showing Trismus and Dysphagia as Early Symptoms

  • Jang, Seo-Hee;Byun, Jin-Seok;Jung, Jae-Kwang;Choi, Jae-Kap
    • Journal of Oral Medicine and Pain
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    • v.45 no.3
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    • pp.79-82
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    • 2020
  • Tetanus is a fatal disease caused by the infection Clostridium tetani found in animal feces and soil. It is a vaccine-preventable disease and rarely occurs in developed countries. However, approximately 30 cases still occur annually in South Korea. Tetanus, commonly called lockjaw, cause contraction of the masseter muscles in the early stage, resulting in trismus as the first symptom. As it progresses, spasm extends to various muscles in the face, neck, shoulder, and back, leading to distorted facial expression, dysphagia, backward arching of the body, dyspnea, and even death. Early diagnosis of tetanus is critical because it can quickly become fatal if left untreated. We present a case of trismus caused by tetanus and emphasize the importance of early diagnosis of acute trismus.

Evaluation of Clinical Signs on Equine Coital Exanthema in Thoroughbred Horses (Thoroughbred 말에 발생한 구진의 임상증상 평가)

  • Yang, Jae-Hyuk;Lee, Kyoung-Kap;Lim, Yoon-Kyu
    • Journal of Veterinary Clinics
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    • v.28 no.4
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    • pp.369-374
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    • 2011
  • Coital exanthema of Thoroughbred horses had occurred firstly in Republic of Korea. This study was performed to evaluate clinical features of coital exanthema, and provide veterinary knowledge to equine practitioner as well as horse farms. The method was clinical, clinical pathology and sexual hormone, electron microscope and libido examination. Five stallions of 27, 45 broodmares of 416 showed clinical symptoms but 124 foals and a teaser did not show any abnormal appearance. Five stallion had papule, pustule, crust around a glans, body of penis, prepuce and remain decolorized spot after treatment. One laceration of glans and 3 gait disorder had been observed but it did not affect libido. Forty-five broodmares localized had papule, pustule, crust, and ulceration around perineal region, vulva, mucocutaneous junction remain decolorized spot after treatment.

Uncertainty Analysis in Potential Transformer Calibration Using a High Voltage Capacitance Bridge

  • Jung, Jae-Kap;Lee, Sang-Hwa;Kang, Jeon-Hong;Kwon, Sung-Won;Kim, Myung-Soo
    • Journal of Electrical Engineering and Technology
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    • v.2 no.3
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    • pp.401-407
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    • 2007
  • Precise absolute measurement of the errors in a potential transformer (PT) can be achieved using high voltage capacitance bridge (HVCB) and capacitive divider. The uncertainty in a PT measurement using the HVCB system was evaluated by considering the overall factors affecting during the calibration of a PT. The expanded uncertainties are found to be not more than $30{\times}10^{-6}$ for ratio and $30{\mu}rad$ for phase up to the primary voltage of $V_p=22kV$. For same PTs, the measured errors in KRISS (Korea Research Institute of Standards and Science) using our bridge are well coincide with those in NMIA (National Measurement Institute of Australia) and PTB (Physikalisch-Technische Bundesanstalt) within the corresponding uncertainties.

Development and Verification of Evaluation Technique for High Voltage Capacitance Bridge (고전압 전기용량 브리지 평가 기술 개발 및 검증)

  • Kim, Yoon-Hyoung;Han, Sang-Gil;Jung, Jae-Kap;Han, Sang-Ok
    • Proceedings of the KIEE Conference
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    • 2009.07a
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    • pp.2059_2060
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    • 2009
  • 본 연구에서는 표준 용량기와 표준 저항을 이용하여 전기용량 브리지의 손실계수와 커패시턴스 비를 측정하여 전기용량 브리지를 평가하는 방법을 개발하였다. 이를 적용하면 기존의 해외 인증기관에서 성능평가를 의뢰했을 때 소요되는 교정기간을 단축하고, 교정 비용을 감소시킬 수 있다. 또한 현장에서 사용되고 있는 장비를 해외 인증기관에 교정 의뢰할 경우 그 기간 동안 장비를 사용할 수 없는 문제를 해결할 수 있다. 측정 범위는 손실계수의 경우 $1{\times}10^{-6}{\sim}7.5{\times}10^{-3}$의 범위까지 광범위하게 측정하였고, 커패시턴스 비는 $C_N{\times}0.1{\sim}C_N{\times}10^3$�劉沮� 측정하였다. 이로부터 제조사에서 제공하고 있는 전기용량 브리지의 성능을 확인이 가능하게 되었다.

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Pharmaceutical Study on Ampicillin-polyvinylpyrrolidone Coprecipitate (Ampicillin-polyvinylpyrrolidone 공침물(共沈物)에 관한 약제학적(藥劑學的) 연구(硏究))

  • Kim, Johng-Kap;Eam, Jin-Soop
    • Journal of Pharmaceutical Investigation
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    • v.10 no.4
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    • pp.23-29
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    • 1980
  • Ampicillin-polyvinylpyrrolidone coprcipitates, 1 : 5 ratio w/w, were prepared by the solvent method to increase the solubility and dissolution rate of ampicillin anhydrous. It was found that the solubility and dissolution rate of the coprecipitate were higher than that of ampicillin anhydrous, mechanical mixture of ampicillin and polyvinylpyrrolidone in powder state. The intrinsic, dissolution rate, G, are $1.15{\times}10^{-4}\;mole\;cm^{-2}\;min^{-1}$ for the coprecipitate, $1.09{\times}10^{-5}\;mole\;cm^{-2}\;min^{-1}$ for ampicillin anhydrous, and the activation energy of the coprecipitate and ampicillin anhydrous are $9.091{\times}10^3cal/mole$ and $1.854{\times}10^3cal/mole$, respectively.

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Application of multi dimensional NMR experiments to VBS RNAs of Yeast Saccaromyces cerevisiae virus

  • Chaejoon Cheong;Cheong, Hae-Kap;Yoo, Jun-Seok
    • Journal of the Korean Magnetic Resonance Society
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    • v.5 no.1
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    • pp.29-36
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    • 2001
  • The structures of two VBS (viral binding site) RNAs, SL1 and SL2, of Yeast Saccharomyces cerevisiae vims have been studied by 2D and 3D NMR experiments. VBSs play a crucial role in viral particle binding to the plus strand and packaging of the RNA. The secondary structures of the two VBS RNAs share a common feature of the stem-internal loop-stem-hairpin loop structure although the size of the internal loops of SL1 and SL2 differs. 2D experiments were sufficient for fill assignments of SL1. However, isotope labeling of the sample and multidimensional experiments were required for 28-nucleotide-long SL2 due to the spectral overlap. Several 3D HCCH experiments have accomplished full assignment of SL2 RNA.

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Structural Studies on IRES 4-2 Domain of Foot-and-mouth Disease Virus

  • Kim, Young-Mee;Yoo, Jun-Seok;Cheong, Hae-Kap;Lee, Chul-Hyun;Cheong, Chae-Joon
    • Journal of the Korean Magnetic Resonance Society
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    • v.7 no.2
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    • pp.89-97
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    • 2003
  • Foot-and-mouth disease virus (FMDV) belongs to the aphthovirus genus within the picornavirus which has a single copy of a positive sense RNA. The translation initiation process of FMDV occurs by a cap-independent mechanism directed by a highly structured element (∼435 nt) termed an internal ribosome entry site (IRES). We have designed and prepared FMDV 4-2 RNA (28nt) by in vitro transcription. The 2D NMR data revealed that FMDV 4-2 IRES domain RNA has a flexible loop and bulge conformation. In further study, we need to make an isotope labeled RNA sample and conduct 3D NMR experiments to completely determine the 3D structure. This study may establish a new drug design strategy to treat foot-and mouth disease.

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Redundant Power System using Three-Phase Diode Rectifier of DC Distribution System for Building Applications (빌딩용 DC배전 시스템의 3상 다이오드 정류회로를 이용한 리던던트 전력 시스템)

  • Park, Yun-Wook;Shin, Soo-Cheol;Lee, Hee-Jun;Won, Chung-Yuen;Na, Jong-Kuk;Kim, Jin-Hong
    • Proceedings of the KIPE Conference
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    • 2012.11a
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    • pp.247-248
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    • 2012
  • 본 논문에서는 빌딩용 직류배전에 적용된 리던던트 전력 시스템을 제안하였다. 제안한 시스템 방식은 3상 다이오드 정류회로를 AC/DC 컨버터와 병렬로 연결하여 전력변환장치의 유지보수 시 직류전원의 무정전 공급이 가능하도록 하였다. 이에 따라 제안된 시스템의 구성에 대하여 기술하고, 시뮬레이션을 통하여 리던던트 전력 시스템의 타당성을 검증하였다.

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An Effect of Multicultural Family Children's Self-Identity to School Adaptation

  • park, Kap Lyong
    • Journal of the Korea Society of Computer and Information
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    • v.21 no.3
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    • pp.123-128
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    • 2016
  • This study aims to analyze the causal relation between identity and school adaptation of multicultural family children, starting from necessity of empirical research considering insufficient preceding research. To achieve the purpose of the research, this study implemented empirical analysis regarding the causal relation between factors of self-identity and school adaptation. As a result, factors of self-identity implies significant effect on school regulations, while factors of school adaptation partially have an effect on school class and relations with a teacher and friend. Based on the result of verification, this study emphasizes teachers' effort as well as comprehensive and practical development of an educational program to help children from multicultural family establish the right self-identity for settling into school life. Moreover, the limitation of this study and future research direction is presented.