• Journal of Microbiology and Biotechnology
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• v.24 no.10
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• pp.1413-1420
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• 2014

Phytate is an antinutritional factor that impacts the bioavailability of essential minerals such as $Ca^{2+}$, $Mg^{2+}$, $Mn^{2+}$, $Zn^{2+}$, and $Fe^{2+}$ by forming insoluble mineral-phytate salts. These insoluble mineral-phytate salts are hydrolyzed rarely by monogastric animals, because they lack the hydrolyzing phytases and thus excrete the majority of them. The ${\beta}$-propeller phytases (BPPs) hydrolyze these insoluble mineral-phytate salts efficiently. In this study, we cloned a novel BPP gene from a marine Pseudomonas sp. This Pseudomonas BPP gene (PsBPP) had low sequence identity with other known phytases and contained an extra internal repeat domain (residues 24-279) and a typical BPP domain (residues 280-634) at the C-terminus. Structure-based sequence alignment suggested that the N-terminal repeat domain did not possess the active-site residues, whereas the C-terminal BPP domain contained multiple calcium-binding sites, which provide a favorable electrostatic environment for substrate binding and catalytic activity. Thus, we overexpressed the BPP domain from Pseudomonas sp. to potentially hydrolyze insoluble mineral-phytate salts. Purified recombinant PsBPP required $Ca^{2+}$ or $Fe^{2+}$ for phytase activity, indicating that PsBPP hydrolyzes insoluble $Fe^{2+}$-phytate or $Ca^{2+}$-phytate salts. The optimal temperature and pH for the hydrolysis of $Ca^{2+}$-phytate by PsBPP were $50^{\circ}C$ and 6.0, respectively. Biochemical and kinetic studies clearly showed that PsBPP efficiently hydrolyzed $Ca^{2+}$-phytate salts and yielded myo-inositol 2,4,6-trisphosphate and three phosphate groups as final products. Finally, we showed that PsBPP was highly effective for hydrolyzing rice bran with high phytate content. Taken together, our results suggest that PsBPP has great potential in the animal feed industry for reducing phytates.

• Bulletin of the Korean Chemical Society
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• v.35 no.9
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• pp.2694-2698
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• 2014

A multisegmented polystyrene (PS) with pH-cleavable ester and carbamate linkages was successfully synthesized by a combination of atom transfer radical polymerization (ATRP) and Cu(I)-catalyzed 1,3-dipolar cycloaddition of azide and alkynes (click chemistry). ATRP was employed to synthesize polystyrene from hydroxyl-terminated initiator using CuBr/N,N,N',N",N"-pentamethyldiethylenetriamine (PMDETA) as the catalyst. The reaction of the resulting PS with sodium azide yielded the azido-terminated polymer. The hydroxyl group in the other end of the polymer was reacted with 4-nitrophenyl chloroformate (NPC), followed by reaction with propargylamine to produce an alkyne end group with a carbamate linkage. The PS with an alkyne group in one end and an azide group in the other end was then self-coupled in the presence of CuBr/2,2'-bipyridyl (bpy) in DMF to yield a desired multisegmented PS. Molecular weight and molecular weight distribution of the self-coupled polymer increased with time, as in the typical step-growth-type polymerization processes. Finally, we demonstrated that the ester and carbamate linkages of the multisegmented PS were hydrolyzed in the presence of HCl to yield individual PS chains.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.47 no.5
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• pp.87-93
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• 2010

This paper presents a high resolution, wide input range 2-step time-to-digital converter used in digital PLL. TDC is used to compare the DPLL output frequency with reference frequency and should be implemented with high resolution to improve the phase noise of DPLL. The conventional TDC consists of delay line realized inverters, whose resolution is determined by delay time of inverter and transistor size, resulting in limited resolution. In this paper, 2-step TDC with phase-interpolation and Time Amplifier is proposed to meet the high resolution and wide input range by implement the delay time less than an inverter delay. The gain of Time Amplifier is improved by using the delay time difference between two inverters. It is implemented in $0.13{\mu}m$ CMOS process and the die area is $800{\mu}m{\times}850{\mu}m$ Current consumption is 12 mA at the supply voltage of 1.2 V. The resolution and input range of the proposed TDC are 0.357 ps and 200 ps, respectively.

• Microbiology and Biotechnology Letters
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• v.46 no.1
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• pp.40-44
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• 2018

An isolate capable of inhibiting the growth of gram-positive bacteria was obtained from the soil of Mushim stream, Cheongju. The isolate was identified as Pseudomonas otitidis PS by 16S rRNA gene sequence analysis. P. otitidis PS produced antibiotics as a secondary metabolite when cultured in 1% soybean meal with 0.5% glucose. The maximum yield was about 0.1%. The antibiotic substance of P. otitidis PS extracted using ethyl acetate displayed a minimum inhibitory concentration of $2{\mu}g/ml$ for Staphylococcus aureus KCTC 1261. The antibiotic substance produced an orange halo on chrome azurol S agar due to siderophore activity. Growth inhibition was decreased when the iron was depleted. Since the antibiotic activity was lost upon the addition of the reducing agent ascorbic acid or during anaerobic culture, it was considered that antibiotic of P. otitidis PS strain exerts its bactericidal effect by the generation of reactive oxygen species.

• Journal of Environmental Science International
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• v.23 no.11
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• pp.1843-1850
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• 2014

PS-D2EHPA beads were prepared by immobilizing di-2-ethylhexyl-phosphoric acid (D2EHPA) with polysulfone (PSf). The removal experiments of Cu(II) and Pb(II) by the prepared PS-D2EHPA beads were conducted batchwise. The removal efficiency of Cu(II) and Pb(II) by PS-D2EHPA beads was increased with increasing pH of solution. The removal rate of Cu(II) and Pb(II) was well described by the pseudo-second-order kinetic model. The maximum removal capacity of Cu(II) and Pb(II) obtained from Langmuir isotherm were 2.58 mg/g and 12.63 mg/g, respectively. External mass transfer coefficients for the removal of Cu(II) and Pb(II) by PS-D2EHPA beads were obtained $0.61{\times}10^{-2}{\sim}5.87{\times}10^{-2}/min$ and $1.55{\times}10^{-2}{\sim}8.53{\times}10^{-2}/min$, respectively and diffusion coefficients were obtained $1.32{\times}10^{-4}{\sim}3.98{\times}10^{-4}cm^2/min$ and $1.80{\times}10^{-4}{\sim}2.28{\times}10^{-4}cm^2/min$, respectively.

• Korean Journal of Psychosomatic Medicine
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• v.10 no.1
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• pp.37-47
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• 2002

Objective : Previous studies have suggested an association between sleep-related breathing disorder (SRBD) and several psychological problems, and there were increasing recognition of the link. The purpose of this study is to evaluate the characteristic profiles of MMPI and SCL-90-R in patients with SRBD. Methods : This study consisted of 80 SRBD patients(73 men, 7 women) referred from Sleep Disorder Clinic of Dong-A University Hospital, Busan, Korea. Basic informations including demographic findings and physical examination were collected. Subjects completed the Epworth Sleepiness Scale(ESS), Minnesota Multiphasic Personality Inventory(MMPI), and Symptom Check List-90-Revision (SCL-90-R) prior to standard overnight polysomnography that was performed at hospital sleep laboratory. SRBD was divided into two groups of primary snoring(PS) and obstructive sleep apnea(OSA) according to polysomnographic findings. Results : SRBD showed significant elevation rate of Hs, D, and Hy scales of MMPI and SOM scale of SCL-90-R, which exceeded the rate expected in normal individuals(>5%, 2SD). On comparison of clinical scales of SCL-90-R, OSA group had significantly greater mean score than that of PS group in terms of O-C, DEP, PAR, GSI(p<0.05), SOM and PST(p<0.01). OSA group also showed significantly higher elevation rate in Hs scale of MMPI and SOM scale of SCL-90-R than that of PS. Among OSA group, three scales of MMPI(D, Pt, Si) and three scales of SCL-90-R(ANX, PAR, PSDI) had significant correlation with some PSG variables including total sleep time and sleep efficiency. Among PS group, two scales of MMPI(Hy and Pt), elevation rate of MMPI scales and three scales of SCL-90-R(I-S, PAR, PSDI) had significant correlation with some PSG variables including sleep efficiency, sleep latency and REM sleep percent. Conclusion : The above results suggest that SRBD show neurotic profiles in MMPI and SCL-90-R. This study also clearly indicates that PS group are suffered from clinically meaningful psychiatric symptoms, which are quantitatively lessened but qualitatively similar as compared to that of OSA group.

• JSTS:Journal of Semiconductor Technology and Science
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• v.16 no.4
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• pp.520-527
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• 2016

A new dual-loop digital delay-locked loop (DLL) using a hybrid (binary + sequential) search algorithm is presented to achieve both wide-range operation and high delay resolution. A new phase-interpolation range selector (PIRS) and a variable successive approximation register (VSAR) algorithm are adopted to resolve the boundary switching and harmonic locking problems of conventional digital DLLs. The proposed digital DLL, implemented in a $0.18-{\mu}m$ CMOS process, occupies an active area of $0.19mm^2$ and operates over a wide frequency range of 0.15-1.5 GHz. The DLL dissipates a power of 11.3 mW from a 1.8 V supply at 1 GHz. The measured peak-to-peak output clock jitter is 24 ps (effective pk-pk jitter = 16.5 ps) with an input clock jitter of 7.5 ps at 1.5 GHz. The delay resolution is only 2.2 ps.

• Proceedings of the Korea Contents Association Conference
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• 2010.05a
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• pp.393-395
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• 2010

본 논문은 PID 필터링된 MPEG-2 TS 데이터로부터 DSM-CC PS 패킷을 추출하여 원래의 섹션으로 구성한 후 이더넷 프레임으로 재구성하는 모듈에 전송하는 패킷변환모듈을 위한 하드웨어 모듈 설계를 제안한다. 제안된 모듈은 ALTERA 사의 IP를 토대로 수정하고 보완한 것으로써 하드웨어 기술언어인 Verilog를 이용하여 설계하였으며 모의실험을 통해 결과를 검증하였다.

• Applied Biological Chemistry
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• v.39 no.1
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• pp.20-24
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• 1996

Pseudomonas fluorescens ps88 was isolated from the rhizosphere soil produced the secondary metabolite called siderophore under iron Limited conditions. On iron limiting KMB medium this strain inhibited the growth of Pythium ultimum, Pyricularia oryzae, Rhizoctonia solani and Xanthomonas oryzae. Cucumber seeds were coated with the strain ps88 and were grown in green house soil. Forty days after the seed emergence, disease incidence caused by Fusarium oxysporium was reduced up to 50%. When the cucumber plants were grown in vermiculite, a significant fresh weight was increased. Root development of red pepper plants was also enhanced on MS medium supplemented with siderophore.

• Culinary science and hospitality research
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• v.18 no.1
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• pp.65-76
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• 2012

As pumpkin is gaining popularity as health functional food among people, this study purposed to prepare powder pumpkin soup of instant food style using ordinary pumpkin (Cucurbita moschata Duch.) that is an oriental pumpkin variety, and sweet pumpkin (Cucurbita maxima Duch.) that is a western pumpkin variety, and to examine its mechanical and sensory characteristics. The viscosity of powder pumpkin soup was higher when the addition ratio of ordinary pumpkin powder was higher than that of sweet pumpkin powder. On the contrary, sugar contents was higher when the addition ratio of sweet pumpkin powder was high. Color did not show any consistent tendency by the addition ratios of ordinary pumpkin powder and sweet pumpkin powder, and lightness was highest in PS2, redness in PS3, and yellowness in PS1. In the results of sensory evaluation through quantitative descriptive analysis, color, glossiness, and the intensity of pumpkin smell were highest in PS3, the intensity of sweetness in PS1, the intensity of saltiness in PS4. In the results of sensory evaluation through palatability test, PS3 showed the highest palatability in terms of appearance, flavor, color, taste, concentration, mouth feel, and overall palatability. Summing up these results, when powder pumpkin soup was prepared at different mixture ratios of ordinary pumpkin powder and sweet pumpkin powder, PS3 showed the most superior mechanical and sensory characteristics. Thus, it is considered most adequate to prepare powder pumpkin soup at the mixture ratio of ordinary pumpkin powder 75% and sweet pumpkin powder 25%.