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An Empirical Study of the Analytical Measurement Range in Clinical Chemistry (분석측정범위의 실증적 평가)

  • Chang, Sang-Wu;Lee, Sang-Gon;Kim, Young-Hwan;Song, Eun-Young;Park, Yong-Won;Park, Byong-Ok;Lyu, Jae-Gi
    • Korean Journal of Clinical Laboratory Science
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    • v.38 no.2
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    • pp.117-124
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    • 2006
  • The analytical measurement range (AMR) is the range of analyte values that a method can directly measure on a specimen without any dilution, concentration, or other pretreatment not part of the usual assay process. The linearity of the AMR is its ability to obtain test results which are directly proportional to the concentration of analyte in the sample from the upper and lower limit of the AMR. The AMR validation is the process of confirming that the assay system will correctly recover the concentration or activity of the analyte over the AMR. The test specimen must have analyte values which, at a minimum, are near the low, midpoint, and high values of the AMR. The AMR must be revalidated at least every six months, at changes in major system components, and when a complete change in reagents for a procesure is introduced; unless the laboratory can demonstrate that changing the reagent lot number does not affect the range used to report patient test results. The AMR linearity was total protein (0-16.6), albumin (0-8.1), total bilirubin (0-18.1), alkaline phosphatase (0-1244.3), aspartate aminotransferase (0-1527.9), alanine aminotransferase (0-1107.9), gamma glutamyl transpeptidase (0-1527.7), creatine kinase (0-1666.6), lactate dehydrogenase (0-1342), high density lipoprotein cholesterol (0.3-154.3), sodium (35.4-309), creatinine (0-19.2), blood urea nitrogen (0.5-206.2), uric acid (0-23.9), total cholesterol (-0.3-510), triglycerides (0.7-539.6), glucose (0-672.7), amylase (0-1595.3), calcium (0-23.9), inorganic phosphorus (0.03-17.0), potassium (0.1-116.5), chloride (3.3-278.7). We are sure that materials for the AMR affect the evaluation of the upper limit of the AMR in the process system.

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THE HISTOLOGICAL STUDY ON INTERFACE BETWEEN BONE AND CHITOSAN COATED DENTAL IMPLANT (키토산피복 임프란트 계면에 관한 조직학적연구)

  • Park, Soo-Ran;Park, Yang-Ho;Park, Young-Ju;Park, Jun-Woo;Lee, Yong-Chan;Ahn, Byoung-Keun
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.28 no.4
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    • pp.302-309
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    • 2002
  • In order to improve the success rate of implants, various implant designs have been developed. Although there have been enough efforts to handle the surface of the implant with careful choice of material and mechanics so that the bone and the implant can be tightly joined together, they have still failed to play the role of periodontal ligaments of the natural teeth in the past. The role of periodontal ligaments is very important since it can improve the initial stability of implant by absorbing the impacts. The purpose of this study is, thus, to test the possibility of alleviating the impact when the surface of the implant was coated with chitosan, a natural polymer, and making sure that the coated material stayed on. Then, the condition of newly developed bone formation and the degree of inflammation in response was closely observed in the surface level. In the main experiment, Chitosan coated implant ($3.3mm{\times}7mm$) was implanted on both the right and the left side of rabbit's femur. The animals were each sacrificed on the $1^{st}$, $2^{nd}$, $3^{rd}$, $7^{th}$, $14^{th}$, $21^{st}$ and 28th day. The process was observed under an light microscope after the Toluidin Blue staining. From the experiment, it was found that the chitosan was evenly distributed on the surface of the screws, and the implant was adjoined with adjacent bone. There was a sign of inflammation on the $3^{rd}$ day, but on the $14^{th}$ day, the formation of woven bone and newly formed bones were noticed. Also, chitosan filled the gap was formed between the implant and the newly formed bone. The implant, the chitosan and the newly formed bone were forming one unit as a result. Therefore, it was found that chitosan coated implant could absorbe the impact in the initial stage of implant.

Clinical Characteristics of Korean Daegu . Kyungpook PCOS Women (대구.경북 지역 한국인 다낭성 난소 증후군 환자의 임상 양상)

  • Bae, Jin-Young;Kim, Mi-Yeon;Sung, Su-Kyung;Oh, Jeong-Eun;Chun, Sang-Sik;Lee, Taek-Hoo
    • Clinical and Experimental Reproductive Medicine
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    • v.36 no.1
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    • pp.71-80
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    • 2009
  • Objective: PCOS is one of the most common endocrine disorder affecting reproductive age women. It shows wide range of reproductive and metabolic manifestations. This study was to determine the differences of clinical and laboratory characteristics between adolescent and adult Korean Daegu Kyungpook PCOS patients. We also compared clinical and laboratory values of Korean PCOS patients to Turkish and American PCOS patients. Methods: 88 cases were diagnosed and enrolled as PCOS patients, based on 2003 Rotterdam criteria, who visited KNUH Reproductive Endocrinology division between Jan. 2000 and Apr. 2008. We retrospectively reviewed clinical characteristics, serum hormone levels, ultrasono-graphic findings. And we divided into 2 groups, adolescent and adult. We analyzed them with Chi-square test and Student's t-test statistically. Results: There were no statistically significant differences between adult and adolescent Korean PCOS patients. The prevalence of hyperandrogenism such as facial acne or hirsutism, obesity, and insulin resistance of Korean PCOS women was significantly lower than Turkish and American PCOS women. Conclusion: From the results of this study, we can suggest that it is sure that there are differences of clinical and laboratory characteristics of PCOS patients between races. Genetic background, dietary habits and life style affect the expression of clinical symptoms of PCOS. But we could not find out the difference between adolescent and adult PCOS patients of same race. It might be due to the limitation of small patient number and narrow range of age distribution. So, there must be large scale and multi-center and multi-regional study.

Exploration of suitable rice cultivars for close mixed-planting with upland-adapted cereal crop

  • Shinohara, Nodoka;Shimamoto, Hitoshi;Kawato, Yoshimasa;Wanga, Maliata A.;Hirooka, Yoshihiro;Yamane, Koji;Iijima, Morio
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.304-304
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    • 2017
  • In semi-arid countries such as Namibia, the flooding unexpectedly happens in a rainy season, causing losses in the yield of upland-adapted cereal crop. In flooding conditions, rice roots sequentially form aerenchyma and a barrier to radial oxygen loss (ROL), and oxygen is released into the rhizosphere near the root tips. Iijima et al. (2016) and Awala et al. (2016) reported that close mixed-planting with rice can mitigate the flood stress of co-growing upland-adapted cereal crop by modifying their rhizosphere microenvironments via the oxygen released from the rice roots. Moreover, by using the model system of hydroponic culture, it was confirmed that oxygen from rice roots was transferred to co-growing upland-adapted cereal crop in close mixed planting system (Kawato et al., 2016). However, it is not sure whether the ability of oxygen release varies among rice cultivars, because Kawato et al. (2016) used only one japonica cultivar, Nipponbare (Oryza sativa). The objective of this study was to compare the ability of oxygen release in rhizosphere among rice cultivars. The experiment was conducted in a climate chamber in Kindai University. We used 10 rice cultivars from three different rice species (O. sativa (var. japonica (2), var. indica (3)), Oryza glaberrima Steud. (2) and their interspecific progenies (3)) to compare the ability of oxygen release from the roots. According to the method by Kawato et al. (2016), the dissolved oxygen concentration of phase I (with shoot) and phase II (without shoot) were measured by a fiber optic oxygen-sensing probe. The oxygen released from rice roots was calculated from the difference of the measurements between phase I and phase II. The result in this study indicated that all of the rice cultivars released oxygen from their roots, and the amount of released oxygen was significantly correlated with the above-ground biomass (r = 0.710). The ability of oxygen release (the amount of the oxygen release per fresh root weight) of indica cultivars (O. sativa) tended to be higher as compared with the other cultivars. On the other hand, that of African rice (O. glaberrima) and the interspecific progenies tended to be lower. These results suggested that the ability of oxygen release widely varies among rice cultivars, and some of indica cultivars (O. sativa) may be suitable for close mixed-planting to mitigate flood stress of upland-adapted cereal crop.

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THE POLYMERIZATION RATE AND THE DEGREE OF CONVERSION OF COMPOSITE RESINS BY DIFFERENT LIGHT SOURCES (광원의 종류에 따른 복합레진의 중합거동 및 중합률에 관한 연구)

  • Ryoo, Joo-Hee;Lee, In-Bog;Yoo, Hyun-Mee;Kim, Mi-Ja;Seok, Chang-In;Kwon, Hyuk-Choon
    • Restorative Dentistry and Endodontics
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    • v.29 no.4
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    • pp.386-398
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    • 2004
  • Objectives: The purpose of this study was to observe the reaction kinetics and the degree of polymerization of composite resins when cured by different light sources and to evaluate the effectiveness of the blue Light Emitting Diode Light Curing Units (LED LCUs) compared with conventional halogen LCUs. Materials and Methods: First, thermal analysis was performed by a differential scanning calorimeter (DSC). The LED LCU (Elipar Freelight, $320{\;}mW/\textrm{cm}^2$) and the conventional halogen LCU (XL3000, $400{\;}mV/\textrm{cm}^2$) were used in this study for curing three composite resins (SureFil, Z-250 and AEliteFLO). Second. the degree of conversion was obtained in the composite resins cured according to the above curing mode with a FTIR. Third, the measurements of depth of cure were carried out in accordance with ISO 4049 standards. Statistical analysis was performed by two-way ANOVA test at 95% levels of confidence and Duncan's procedure for multiple comparisons. Results: The heat of cure was not statistically different among the LCUs (p > 0.05). The composites cured by the LED (Exp) LCUs were statistically more slowly polymerized than by the halogen LCU and the LED (Std) LCU (p < 0.05). The composite resin groups cured by the LED (Exp) LCUs had significantly greater degree of conversion value than by the halogen LCU and the LED (Std) LCU (p =0.0002). The composite resin groups cured by the LED (Std) LCUs showed significantly greater depth of cure value than by the halogen LCU and the LED (Exp) LCU (p < 0.05).

EFFECT OF LIGHT INTENSITY ON THE POLYMERIZATION RATE OF COMPOSITE RESIN USING REAL-TIME MEASUREMENT OF VOLUMETRIC CHANCE (광조사 강도가 복합레진의 중합반응속도에 미치는 영향에 관한 실시간 체적측정법을 이용한 연구)

  • La, Sung-Ho;Lee, In-Bog;Kim, Chang-Keun;Cho, Byeong-Hoon;Lee, Kwang-Won;Son, Ho-Hyun
    • Restorative Dentistry and Endodontics
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    • v.27 no.2
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    • pp.135-141
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    • 2002
  • Objectives : The aim of this study is to evaluate the effect of light intensity variation on the polymerization rate of composite resin using IB system (the experimental equipment designed by Dr. IB Lee) by which real-time volumetric change of composite can be measured. Methods : Three commercial composite resins [Z100(Z1), AeliteFil(AF), SureFil(SF)] were photopolymerized with Variable Intensity Polymerizer unit (Bisco, U.S.A.) under the variable light intensity (75/150/225/300/375/450mW$^2$) during 20 sec. Polymerization shrinkage of samples was detected continuously by IB system during 110 sec and the rate of polymerization shrinkage was obtained by its shrinkage data. Peak time(P.T.) showing the maximum rate of polymerization shrinkage was used to compare the polymerization rate. Results : Peak time decreased with increasing light intensity(p<0.05). Maximum rate of polymerization shrinkage increased with increasing light intensity(p<0.05). Statistical analysis revealed a significant positive correlation between peak time and inverse square root of the light intensity (AF:R=0.965, Zl:R=0.974, SF:R=0.927). Statistical analysis revealed a significant negative correlation between the maximum rate of polymerization shrinkage and peak time(AF:R=-0.933, Zl:R=-0.892, SF:R=-0.883), and a significant positive correlation between the maximum rate of polymerization shrinkage and square root of the light intensity (AF:R=0.988, Zl:R=0.974, SF:R=0.946). Discussion and Conclusions : The polymerization rate of composite resins used in this study was proportional to the square root of light intensity Maximum rate of polymerization shrinkage as well as peak time can be used to compare the polymerization rate. Real-time volume method using IB system can be a simple alternative method to obtain the polymerization rate of composite resins.

An Experimental Study on the Drying Shrinkage of Concrete Using High-Quality Recycled Sand (고품질 순환잔골재를 사용한 콘크리트의 건조수축 특성에 관한 실험적 연구)

  • Song, Ha-Young;Lee, Sang-Soo;Lee, Do-Heun;Lee, Jong-Gou;Kim, Jae-Hwan;Lim, Hyon-Ung
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.2 no.1
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    • pp.136-143
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    • 2006
  • In this study, recently it is urgently required that demolition waste concrete has to be recycled on the construction because urban development is accelerated and redevelopment project is rapidly expanded, production quantity of construction and demolition waste concrete is being increased. As a results of drying shrinkage test under restrained and unrestrained condition, although workability and mechanical properites of concrete using HQRS were similar to that of concrete using natural sand, there were a great difference in deformation characteristic of dry shrinkage according to replacement ratio of HQRS. And, it makes sure that use of HQRS instead of partial nature sand was effective because drying shrinkage of concrete using 30 volume percentage of HQRS was smaller than that using only natural sand. Therefore, it is the objective of this study to provide the fundamental data about the re-application as an analysis of the drying shrinkage characteristics of concrete using HQRS and it is able to creta a high value-added by using HQRS.

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Studies of the possibility of external threats of the automotive ECU through simulation test environment (자동차용 ECU의 CAN 메시지를 통한 자동차 공격 방법 연구)

  • Lee, Hye-Ryun;Kim, Kyoung-Jin;Jung, Gi-Hyun;Choi, Kyung-Hee;Park, Seung-Kyu;Kwon, Do-Keun
    • Journal of the Korea Society of Computer and Information
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    • v.18 no.11
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    • pp.39-49
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    • 2013
  • In this paper, security mechanism of internal network(CAN) of vehicle is a very incomplete state and the possibility of external threats as a way to build a test environment that you can easily buy from the market by the vehicle's ECU(Electric Control Unit) to verify and obtain a CAN message. Then, by applying it to ECU of the real car to try to attack is proposed. A recent study, Anyone can see plain-text status of the CAN message in the vehicle. so that in order to verify the information is vulnerable to attack from outside, analyze the data in a vehicle has had a successful attack, but attack to reverse engineering in the stationary state and buying a car should attempt has disadvantages that spatial, financial, and time costs occurs. Found through the car's ECU CAN message is applied to a real car for Potential threats outside of the car to perform an experiment to verify and equipped with a wireless network environment, the experimental results, proposed method through in the car to make sure the attack is possible. As a result, reduce the costs incurred in previous studies and in the information absence state of the car, potential of vehicle's ECU attack looks.

THE EFFICACY OF PROPHYLACTIC ANTIBIOTICS FOR MANDIBULAR THIRD MOLAR EXTRACTION (하악 제3대구치 발치를 위한 예방적 항생제의 효용성)

  • Kang, Sang-Hoon;Kim, Jung-In;Park, Won-Se;Yi, Choong-Kook;Lee, Sang-Hwy
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.34 no.3
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    • pp.365-369
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    • 2008
  • This study analyzed the incidence of wound infection after the operation of mandibular third molar extraction in relation with antibiotic prophylaxis with the object of young and healthy patients. The study object was 1,177 mandibular third molars of 850 men of 20 to 25 years old without any specific systemic disease. Three methods of preventive antibiotic medication were selected according to the preventive antibiotic medication previously reported; three experimental groups were selected based on them, and the antibiotic used was amoxicillin($^{(R)}Kymoxin$, Yuhanyanghaeng, Seoul). The group 1 includes the patients that took the antibiotic orally before the operation(one hour earlier, 500mg) and for three days after the operation(250mg per time, three times/day), the group 2 is the ones that took the same antibiotic orally only once about one hour before the operation(500mg), and the group 3 did not take any antibiotics before and after the operation. And to compare the difficulties and the degrees of extraction during operations which can be possibly related to the wound infection after the operations, the mandibular third molars' impacted depths and extraction methods were investigated as well. To check if the wound was infected, observations with an internal of one week were performed twice after the operation, and the meaningfulness of the infection incidence was verified through Chi-square test using SPSS program(SPSS Inc., IL, USA). There was no statistically significant difference between the antibiotic medication methods and the wound infection incidence after the operation among the experimental groups. As examining the relations between the mandibular third molar operation methods and the wound infection incidence after the operation, there existed a statistically meaningful difference in the infection incidence according to the operation methods(p=0.020). And there was no statistically significant difference in the wound infection incidence according to the impacted depth of the mandibular third molar. Therefore, it is thought that there exists little necessity of prophylatic antibiotics medication when extracting the mandibular third molar of young and healthy men without any systemic disease in general; however, in case when it is expected that the possibility of infection will be high or the wound on the tissue will be severe, it is sure that the prophylactic antibiotics medication will be necessary.

Usefulness of CT based SPECT Fusion Image in the lung Disease : Preliminary Study (폐질환의 SPECT와 CT 융합영상의 유용성: 초기연구)

  • Park, Hoon-Hee;Kim, Tae-Hyung;Shin, Ji-Yun;Lee, Tae-Soo;Lyu, Kwang-Yeul
    • Journal of radiological science and technology
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    • v.35 no.1
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    • pp.59-64
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    • 2012
  • Recently, SPECT/CT system has been applied to many diseases, however, the application is not extensively applied at pulmonary disease. Especially, in case that, the pulmonary embolisms suspect at the CT images, SPECT is performed. For the accurate diagnosis, SPECT/CT tests are subsequently undergoing.However, without SPECT/CT, there are some limitations to apply these procedures. With SPECT/CT, although, most of the examination performed after CT. Moreover, such a test procedures generate unnecessary dual irradiation problem to the patient. In this study, we evaluated the amount of unnecessary irradiation, and the usefulness of fusion images of pulmonary disease, which independently acquired from SPECT and CT. Using NEMA PhantomTM (NU2-2001), SPECT and CT scan were performed for fusion images. From June 2011 to September 2010, 10 patients who didn't have other personal history, except lung disease were selected (male: 7, female: 3, mean age: $65.3{\pm}12.7$). In both clinical patient and phantom data, the fusion images scored higher than SPECT and CT images. The fusion images, which is combined with pulmonary vessel images from CT and functional images from SPECT, can increase the detection possibility in detecting pulmonary embolism in the resin of lung parenchyma. It is sure that performing SPECT and CT in integral SPECT/CT system were better. However, we believe this protocol can give more informative data to have more accurate diagnosis in the hospital without integral SPECT/CT system.