• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.12
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• pp.1647-1653
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• 2008

This study used response surface methodology (RSM) in an effort to optimize the water extraction conditions of Hypsizigus marmoreus in order to increase cytotoxicity activity of the extract. A central composite design was applied to investigate the effects of independent variables, which included the extraction temperature ($X_1$), extraction time ($X_2$), the ratio of solvent to sample ($X_3$) on dependent variables of the extracts, including extraction yield ($Y_1$) and protein content ($Y_2$). The estimated optimal conditions were as follows: $51.3^{\circ}C$ extraction temperature, 8.2 hrs extraction time, and 46.7 mL/g of solvent per sample. The extract (CE) was extracted at optimal condition and crude polysaccharides (CPS) were obtained from CE by ethanol precipitation, dialysis, and freeze drying. Neutral (NPS) and acidic (APS) fraction of polysaccharides were seperated from CPS by ion chromatography. The growth inhibitory effects of the APS (0.5 mg/mL) on AGS human cancer cells were 73.97%. CPS showed the highest growth inhibitory effects on HepG2 human cancer cell at 0.5 mg/mL. However all fraction polysaccharides from Hypsizigus marmoreus showed lower than 20% growth inhibition on SW480 human cancer cell.

• Korean Journal of Soil Science and Fertilizer
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• v.20 no.1
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• pp.35-41
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• 1987

Nitrate nitrogen was absorbed dominantly among the inorganic nitrogen nutrients by tobacco plant. Transport and reduction of $NO_3-N$ in plant tissue were the important metabolism for supplying synthetic N compounds to developing tissues during growth period. Under field and environment-controlled condition tobacco plants were grown and seperated to leaf tissues at stalk positions for investigation of nitrogen transport and assimilation ability during period of rapid vegative growth. The results of studies were summarized as follows: 1. $NO_3-N$ absorbed from roots was transported as inorganic nitrogen through the vascular tissue of leaf veins as resulting from the high $NO_3-N$ ratio of the nitrogen content in leaf veins, but these ratios in mesophyll tissue of the same leaf laminae decreased remarkably in disregard of higher accumulation of nitrogen being compared to midvien. 2. Mesophyll tissue of mature leaves appeared higher value of nitrate reductase activity (NRA) comparing with other tissues, stem, leaf vien, and meristmatic tissue at emergence point with young leaves. 3. Matured leaves at lower position being reducing nitrate nitrogen vigorously observed thick laminae and kept high amount of water in them. 4. Mature leaves of young plant reduced $NO_3-N$ vigorously for supply synthetic N compounds to meristmatic tissues at growing point by the reason of narrow and few leaves at young stage, but in advancing growth period NRA of mature leaves along upper position reached to lower value. This appearence attributed to distribution of organic-N compound demanding for growth to increasing numbers of wide leaves.

• Journal of muscle and joint health
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• v.1 no.1
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• pp.97-115
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• 1994

The purpose of this study was to examine the reliability and validity of the observed behaviors of subjects who suffered from low back pain with spinal diseases, Thirty two low back pain patients admitted on the neurosurgical unit in an army hospital were compared with 30 normal controls belonged to an army unit, by means of matching the age, hight and weight. Observed pain behaviors were developed by the researcher on the bases of literature and patient observation. This tool consists of 18 behaviors seperated into two major groups : mutually exclusive and concomittent behaviors. The mutually exclusive behaviors included coding cathegories for 6. body motions assumed by the subjects during the observation session. These 6 standardized motions consisted of sitting from standing first, and serially tying down, reclining, sitting again, and then standing, 6 steps walking. Concomittent behaviors consisted of 12 observable patterns that can be observed systematically from the face, grimacing, bracing, rubbing, walking with arms fixed, support with hands on sitting or standing, guarded movement, limping, unbalaced weightbearing, stopped movement from tying position to sitting, sighing and graoning. Subjects were videotrecorded as they performed a 6-standardized sequence of motions, simultanously researcher measured the time spent performing each motion and step length. Patients were asked torate their subjective pain score on the 10 mm graphic rating scale ranging from 'no pain' to 'sever pain'. For scoring of the pain behaviors, two trained nursing officiers independently and simutanously viewwd each videorecording and checked subject 'pain behaviors at the observational item checklist. The result of the study are summarized as follows : 1. Reability of the observational tool was a=.845. 2. Spearman's rho and percentage agreement were p=.97 and 81.7 persent respectively, that indicate adequate interrater reability of this tool. 3. The sensitivity rate of the tool was .875 while specificity rate .866 for differentiating patient from the normal. 4. When difference in the objective pain indices between patient group and control were compared, there was significant difference of all indices, such as pain behavior(t=7.71, p=.0001). spent time performing motion(t=14.2, p=.0001), step length (t=-10.72, p=.0001). 5. There were differences in the objective indices the subjective pain subgroups (low, medium, high). Differences in the mean score of objective pain behavior (F=6.376. p=.005) and spent time for moyion(F=4.631, p=.018). But there were no significant differences in the step length among the subgroups(F=.667, p=.521). 6. Highly correlated pain behavior items wiyh subjective pain score were 'stopped movement from lying position to sitting', 'limping', 'support with hands on sitting or standing', 'bracing', 'guarding' and 'walking with arms fixed'. In summary, although some of rho behavior items such as sighing and groaning in this study could not be observed because of videotaped datd, the reliability and validity of the over all observation method were satifactory. Thus, the results of the present study demonstrate rye potetional utility of the tool in assessing objective pain complementing self-reported pain in low back pain patients.

• Journal of Food Hygiene and Safety
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• v.35 no.5
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• pp.513-520
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• 2020

Here, we evaluated the functional properties of histidine-containing low-molecular-weight (LMW) peptides obtained from tuna waste meats. As with histidine-related components composed of histidine, 1-methyl histidine and anserine, histidine-containing LMW peptides exhibited high α,α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging effect in a dose-dependent manner. Among the histidine-related dipeptides, anserine exhibited the highest reducing power followed by carnosine. By comparison with dipeptides, tuna extracts also showed similar reducing power and the activity was in a dose-dependent manner. In addition, the antioxidant activities of tuna extracts such as DPPH radical scavenging effect, reducing power, superoxide dismutase activities, and peroxide value of linoleic acid were affected by the various extraction methods.

• Journal of Radiation Industry
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• v.4 no.3
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• pp.221-226
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• 2010

Physical detection methods, photostimulated luminescence (PSL), thermoluminescence (TL) and electron spin resonance (ESR) were applied to detect electron beam-irradiated agricultural products, such as red pepper, black pepper, raisin, walnut, beef seasoning and pistachio. The absorbed irradiation doses for representative samples were controled at 0, 1, 3, 5 and 10 kGy. PSL values for non-irradiated samples were <700 counts/60s (lower threshold, $T_1$) except beef seasoning, whereas those of irradiated samples were more than 5,000 photon counts, upper threshold ($T_2$) in black pepper, raisin, and beef seasoning and intermediates values of $T_1-T_2$ in red pepper, walnut, and pistachio. Minerals seperated from the samples for TL measurement showed that non-irradiated samples except pistachio (TL ratio, 0.12) were characterized by no glow curves situated at temperature range of $50{\sim}400^{\circ}C$ with TL ratio (0.01~0.08), while irradiated samples except pistachio at only 1 kGy (TL ratio, 0.08) indicated glow curve at about $150{\sim}250^{\circ}C$ with TL ratio (0.28~3.10). ESR measurements of irradiated samples showed any specific signals to irradiation. The samples of both red pepper and pistachio were produced specific signals derived from cellulose radicals as well as single line signals for black pepper and walnut, and multiple signals derived from crystalline sugar radicals for raisin and beef seasoning. In conclusion, The ESR methods can apply for detection of pistachio exposed to electron beam but PSL and TL are not suitable methods. Furthermore, TL and ESR suggeted that both techniques were more useful detection method than PSL to confirm whether red pepper, walnut and beef seasoning samples have been exposed to electron beam.

• Applied Microscopy
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• v.35 no.4
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• pp.24-31
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• 2005

The preservative condition and ultrastructure on the mummified hair collected from newly found female mummy in Paju, were investigated by using scanning and transmission electron microscopes. The female mummy was found in september, 2002 during the traditional reburial process for the buried ones. The hair of 16th century mummy showed very intact appearances during observation with electron microscope. And the structures of the cortex, medulla and cuticle were well preserved. The cuticle layer was easily discernable, which are composed of six to seven cuticular cells. Each cuticular cells surrounded and thus seperated from its neighbors by intercellular membrane complex. In the cortex, many macrofibrils and some melanin granules between them were observed. We observed well preserved rod form macrofibrils running parallel along the direction of hair shaft. Especially, melanin granules were aggregated in the cortex which was adjacent to the cuticlu layer. As to the cause for the well-preservation of 440 year old hair sample, the presence of surface coat on the hair, which are composed of various materials. As calcium was included in the surface coat in Electron Dispersive X-ray Spectroscopy (EDS), the hardening process of the surface coat by calcium might inhibit the water or microorganism infiltration into the hair.

• Journal of architectural history
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• v.9 no.1 s.22
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• pp.7-25
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• 2000

The purpose of this study is focused to the historic changes of Yungnam-Ru(嶺南樓), which are based on the site layout, and architectural forms in architectural building history and historic periods analyzed with the historic reference, paintings, and photos. This study is to search the alteration of the types of axis and the formation of spaces in Yungnam-Ru which is the Ru-Gak(樓閣) The conclusion of the architectural changes from the historic period and architectural form which is the belows. The first Yungnam-Ru by name had been used to be called, before it was re-called Yungnam-Ru by Kim Ju in 1365. Therefore, the hypothesis in naming Yungnam-Ru form assumption that the building under the name of Yungnam-Ru was re-named by Kim Ju from the Old Budist Temple called Yungnam-Sa, should re-considered in history. The second, it is considered that Milyang-Sibyi-Kyungdo(密陽十二景圖) as the painting can only be seen the site layout in 1542. It could be compared the differences of the site layouts from in 1542 to the present time. At that time Nyungpadang(凌派堂) was connected the main buildings called Yungnam-Ru Chimrudang(沈流堂),building was seperated with the Yungnam-Ru at that period. In 1542, the main Building(Yungnam-Ru) was consist of 5 spans of columns(from the front) and 2 spans of columns(from the side). Now, the main Building(Yungnam-Ru) has the 5 spans of columns from the front, and two spans of columns short from the side, compared to the present facade. At the past, Chimrudang(building) has the two spans of columns and one span of columns short, compared to the present facade. The third, It supposed that main building, Nyungpadang and Chimrudang in the composite of facade was connected with Wolrang(月廊) and Hunrang(軒廊) after invatioin from japan in 1592. 1844, (Chosun dynasty, Hunjong 10) the Yungnam-Ru was re-builted by maintaining the same concept in site layout of the past, and finally the three buildings was put together with Wolrang and Hunrang. As a result, the plan of the Yungnam-Ru was expanded with many aspects. From 1542 to 1844, the present site-layout gradually completed with three buildings which was spatially connected. The forth, in the middle age of Chosun dynasty, after added Gaeksa(客舍), the building is for the government officer staying temporally from outside province) in the site, the site layout was greatly changed with volume of building. In 1844, the Yungnam-Ru as the Nugak belongs to Miljukwan(密州館) was expanded spacially and formally. After that time, the burned buildings could not have been re-built because of aspects in government ability and economical ability.

• Food Science and Preservation
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• v.11 no.4
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• pp.496-502
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• 2004

Purified acorn starch was obtained from alkali precipitation method. Acorn amylose and acorn amylopectin were fractionated from purified acorn starch by butanol improvement method. Gel permeation chromatography (GPC) was used to measure molecular weight distribution of acorn starch, acorn amylose, acorn amylopectin and corn starch, corn amylose, corn amylopectin. GPC measurement diagrams were obtained by each retention time. And then, we used DMSO and DMF as solvent, pullulan as standard material. We calculated the Number-average molar mass (Mn), Weight-average molar mass (Mw) and polydispersity from molecular weight distribution of each sample. As a result of estimating molecular weight using GPC, Mw of amylose has small value than Mw of amylopectin. From this fact, the molecular structural aspects of amylose and amylopectin were predicted and it was in good agrement with the tendency of polydispersity by GPC. The polydispersity of starch had big value than amylose and amylopectin, from this result, it might be known that the range of molecular weight appeared broad by heterogeneous properties of two components. The viscosity of purified acorn starch, amylose, amylopectin seperated from acorn starch, was decreased by increasing the shear rate and raising the temperature exponentially. Acorn starch solutions exhibited pseudoplastic power law fluid behavior.

• The Korean Journal of Pharmacology
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• v.18 no.2
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• pp.33-44
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• 1982

The binding in vitro of an opiate agonist, $(^3H)-morphine$, was studied using rat brain slices which were incubated in the modified Krebs-Henseleit bicarbonate buffer solution containing various concentrations of electrolytes with or without morphine, naloxone or morphine+naloxone at $4^{\circ}C$ for 24 hours. The binding of $(^3H)-morphine$ may be seperated into two component; one a saturable binding and the other nonsaturable. The saturable binding may be calculated from the differences in binding observed in the absence and presence of high concentration of morphine. The maximal saturable binding and $K_D$ value in the naive preparations were $0.32{\pm}0.02\;pmole/mg$ protein and $0.75{\pm}0.07\;nM$ respectively. The saturable binding of $(^3H)-morphine$ was significantly increased by low temperature-treatment, while $K_D$ value was not changed. Morphine in the incubation media significantly increased the saturable binding of $(^3H)-morphine$ and $K_D$ value. Naloxone also increased the maximal saturable binding of $(^3H)-morphine$ and $K_D$ value of the drug. Decrease of $K^+\;and\;Mg^{++}$, and addition of $Mn^{++}$ in the incubation media significantly increased the saturable binding of $(^3H)-morphine$, but decrease of $Na^+$and increase of $Ca^{++}$ in the incubation media did not influence the binding. The increment of the saturable binding of $(^3H)-morphine$ by nonlabeled morphine in the incubation media was notaffected by decrease of $Na^+,\;K^+\;or\;Mg^{++}$, or addition of $Mn^{++}$ into the incubation media, but was inhibited by increase of $Ca^{++}$ in the incubation media, while the increment of the saturable binding of $(^3H)-morphine$ was net observed by decrease of $Na^+,\;K^+\;or\;Mg^{++}$, or increase of $Ca^{++}$ in the incubation media. The above results indicate that change of opiate binding sites in quality, i.e. affinity, and quantity, i.e. number of binding sites, may occur by low temperature-treatment in the absence and presence of morphine or naloxone and that electrolytes play role of the changes of opiate binding sites.

• The Korean Journal of Nuclear Medicine Technology
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• v.18 no.1
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• pp.3-9
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• 2014

Purpose: Our goals were to evaluate the effect of high dose radioiodine treatment for thyroid cancer by taking in laxatives. Materials and Methods: Twenty patients(M:F=13:7, age $46.3{\pm}8.1\;yrs$) who underwent high dose radioiodine treatment were seperated into Group 1 taking $^{131}I$ 5,500 MBq and Group 2 with the use of laxatives after taking $^{131}I$ 5,500 MBq. The whole body was scanned 16 hours and 40 hours after taking radioactive iodines by using gamma camera, the ROIs were drawn on the gastro-intestinal tract and thigh for calculation of reduction ratio. At particular time during hospitalization, the radioactivity remaining in the body was measured in 1 meter from patient by using survey meter (RadEye-G10, Thermo Fisher Scientific, USA). Schematic presentation of an Origin 8.5.1 software was used for spatial dose rate. Statistical comparison between groups were done using independent samples t-test. P value less than 0.05 was regarded as statistically significant. Results: The reduction ratio in gastro-intestinal 16 hours and 40 hours after taking laxatives is $42.1{\pm}6.3%$ in Group 1 and $72.1{\pm}6.4%$ in Group 2. The spatial dose rate measured when discharging from hospital was $23.8{\pm}6.7{\mu}Sv/h$ in Group 1 and $8.2{\pm}2.4{\mu}Sv/h$ in Group 2. The radioactivity remaining in the body is much decreased at the patient with laxatives(P<0.05). Conclusion: The use in combination with laxatives is helpful for decreasing radioactivity remaining in the body. The radioactive contamination could be decreased at marginal individuals from patients.