The second leaves from 30 days old seedlings of two rice cultivars which were selected as tolerant (cv. Chokoto) and susceptible (cv. Weld pally) cultivar were soaked in the concentration of $10^{-6}$, $10^{-5}$, $10^{-4}$ and $10^{-3}M$ of oxyfluorfen for 10, 15 and 22hrs and anatomical characteristics were abserved. Dipping to the solutions were carried out either directly to the attached leaves or to the seperated leaves. Development of any symptoms in epidermis, bundle sheath, mesophyll cells and bulliform cells were microscopically inspected. Both cultivars showed reductionin leaf thickness, but the susceptible ones was more sensitive than the tolerant. The degradation and disappearance of epidermal cell layer, breakage of bundle sheath cells, shrinkage of mesophyll cells and disappearance of bulliform cells were general response as affected by oxyfluorfen treatment. The susceptible cultivars showed such responses at the concentration of $10^{-5}M$ for 10 hrs while tolerant ones $10^{-3}M$, for 10 hrs. Those treatments were more effective in seperated leaves than in attached ones. The epicuticular wax layer of leaves treated as above for 20 hrs was inspected by SEM. Weld pally, the susceptible cultivar (Weld pally) showed rapid cleavage of wax layer under $10^{-5}M$ concentration while the tolerant (Chokoto) showed only minor damage on wax layer at the concentration of $10^{-3}M$.
The author has conducted survey on the socio-medical status of the 157 entertainers (male:25, female:132) in Jeonju area, during 1 to 15 July 1974. As results of this survey, following conclusions were obtained. 1. The age distribution was revealed the peak in the age group of 20 to 24 years with 44.5 per cent. Average age of male entertainers was 28.0 years and female entertainer with 23.9 years. 2. By the educational background of the entertainers, the greatest proportion or 45.9 per cent was middle school, 28.7 per cent in primary school. 3. Most of the entertainers had no their parents, father or mother while the ones who had parents were 21.0 per cent. 4. The average age of menerache of the female entertainers was $14.2{\pm}0.87$ years. 5. Regarding the marital status of the entertainers, the unmarried were 81.5 per cent and present status of marriage, 79.3 per cent were seperated. 6. The average of the first marriage was 24.4 years in male, and 22.6 years in female. 7. Regarding the working duration of the entertainers, 79.1 per cent worked less than 1 year. 8. Regarding the sex experiences before marriage, 76.0 per cent of the male and 91.7 per cent of the female reported had such experiences. 9. Induced abortions were found to have been experienced 88.6 per cent of the female entertainers. 10. Point prevalence rate for gonorrhea including non-specific urethritis of the entertainers were found 16.0 per cent of the male and 17.4 per cent of the female.
Ye, Qing;Ding, Shao-Feng;Wang, Zhi-An;Feng, Jie;Tan, Wen-Bin
Asian Pacific Journal of Cancer Prevention
/
v.15
no.12
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pp.4995-5000
/
2014
Background: Cancer constitutes a key pressure on public health regardless of the economy state in different countries. As a kind of highly malignant epithelial tumor, lacrimal gland adenoid cystic carcinoma can occur in any part of the body, such as salivary gland, submandibular gland, trachea, lung, breast, skin and lacrimal gland. Chemotherapy is one of the key treatment techniques, but drug resistance, especially MDR, seriously blunts its effects. As an element of the 60S large ribosomal subunit, the ribosomal protein L39-L gene appears to be documented specifically in the human testis and many human cancer samples of different origins. Materials and Methods: Total RNA of cultured drug-resistant and susceptible lacrimal gland adenoid cystic carcinoma cells was seperated, and real time quantitative RT-PCR were used to reveal transcription differences between amycin resistant and susceptible strains of lacrimal gland adenoid cystic carcinoma cells. Viability assays were used to present the amycin resistance difference in a RPL39-L transfected lacrimal gland adenoid cystic carcinoma cell line as compared to control vector and null-transfected lacrimal gland adenoid cystic carcinoma cell lines. Results: The ribosomal protein L39-L transcription level was 6.5-fold higher in the drug-resistant human lacrimal gland adenoid cystic carcinoma cell line than in the susceptible cell line by quantitative RT-PCR analysis. The ribosomal protein L39-L transfected cells revealed enhanced drug resistance compared to plasmid vector-transfected or null-transfected cells as determined by methyl tritiated thymidine (3H-TdR) incorporation. Conclusions: The ribosomal protein L39-L gene could possibly have influence on the drug resistance mechanism of lacrimal gland adenoid cystic carcinoma cells.
Objective : There have not been studies on the oriental medicine research trends and qualitative analysis of Korean oriental medicine articles in Korea, particularly in the oriental medicine area. This study's purpose is to appreciate Korean oriental medicine's research trends and assess the quality of the Korean oriental medical journals' level. This study will help upgrade Korean oriental medical journals. Method : We found 93 stroke-related articles in the Journal of Korean Oriental Medicine, published between 1980(Volume 1) and 2001(Volume 45). We surveyed the institutes involved in each study, and the number of people in each study. We seperated by epidemiological study design, the studies into categories. Campbell and Stanley's study design into 6 categories, statistical methods into 13 categories. Next, we assessed the 41 articles by a checklist, which had 10 categories. The quality score was analyzed by survey variables over the period, which were divided by the date of publication: the 1980s, the first half of the 1990s, second half of the 1990s and 2000s. Results : 46.3% od the studies didn't use statistical methods. 56.1%, were case series studies by classification of epidemiological study design, and 53.6% were one-shot case studies designed by Campbell and Stanley's study design. The quality score was 58.33 point 60 is average. In quality level by Browman GP etc.'s, 83.0% of the studies were included in V levels. But, the quality scores were significant by presence of reviewer system, statistical method's usage, and over the period. Conclusion : The Journal of Korean Oriental Medicine needs to improve the quality its articles. To improve the quality of oriental medicine related articles, the following are necessary: working together, joint work of inter-educational system, strengthening of reviewer system, use of statistical methods, and consulting to the expert of research at the stage of study design.
Objective : This study was carried out to investigate the effects of DDT on the brain damage and hypertension. Methods : We observed the effect of Dodamtang(DDT) extract on KCN-induced coma, focal brain ischemia by MCA occlusion, cytotoxicity and protection of PC12 cells and B103 cells induced by amyloid ${\beta}$ protein(25-35). To prove the effect of DDT as a blood pressure depressant, we measured aldosterone, renin activity, catecholamine, sodium and NO density using the seperated blood plasma. Results : DDT showed a protective effect on cytotoxicity of PC12 cells and B103 cells induced by amyloid ${\beta}$ protein(25-35) in a dose dependent manner and proved the significant abridgement of brain ischemic area and edema induced by MCA occlusion, a critical decrease of neurologic deficitic grade in the fore-limbs. DDT didn't reduce the duration of KCN(1.87mg/kg iv.)-induced coma and prolonged the survival rate in the case of KCN(3.0mg/kg iv.)-induced coma by the ratio of 20%. While DDT increased the value of NO in SHR, it significantly decreased the blood pressure of SHR and the value of aldosterone& epinephrine in SHR. Conclusions : These results suggested that DDT might be usefully applied for treatment of hypertension, cerebral infarction, and brain damage.
This study was conducted to obtain the basic data for using the Artemisia genetic resources as a medicinal crop. 24 taxa including Artemisia capillaris Thunb. were analyzed by principal component analysis of 25 characters and cluster analysis for classification. In Principal components analysis of individuals of taxa using 25 morphological characters of reproductive organ, the first, the second, the third and the fourth components contributed 44.73%, 16.86%, 8.88%, and 7.07% of the variations, respectively. The cumulative contribution from the first to the fourth principal components was 77.56%. In cluster analysis, taxa of Artemisia L. was seperated 3 group by 25 morphological characters of reproductive organ, but it didn't completely coincident with Kitamura classification.
The purpose of this study is to observe activation of limbic system during performing visuospatial tasks by 21% and 30% oxygen administration. Eight right handed male college students were selected as the subjects for this study. A visuospatial task was presented while brain images were scanned by a 3T fMRI system. The experiment consisted of two runs: one was a visuospatial task under normal air(21% oxygen) condition and the other under hyperoxic air(30% oxygen) condition. The neural activations were observed at the limbic system which is seperated 8 regions such as cingulate gyrus, thalamus, limbic lobe, hypothalamus, hippocampus, parahippocampa gyrus, amygdala, and mammiilary body. By two oxygen levels, activation areas of limbic system are almost identical. Increased neural activations were observed in the cingulate gyrus and thalamus with 30% oxygen administration compared to 21% oxygen. During 30% oxygen administration, improvement of visuospatial task performance has a relation to increase of neural activation of subcortical structures such as thalamus and cingulate gyrus as well as cerebral cortex.
Seo, Hyun-Soo;Lee, Young-Joo;Byeon, Kwang-Seob;Hong, Soon-Min;Park, Jun-Woo;Hong, Ji-Sook;Park, Yang-Ho
Maxillofacial Plastic and Reconstructive Surgery
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v.33
no.1
/
pp.55-61
/
2011
Conventional slinding genioplsty has the risk of mental nerve injury after operation and difficult to correct vertical asymmetry of chin. So, authors propose a new genioplasty to correct asymmetry of chin. Switching genioplasty is a modification method of conventional genioplasty. Between mandibular right and left canine, osteotomy line of triangular shape make until mandibular lower border. In large side, osteotome line of wedge shape is added to reduction. After osteotomy, segment of wedge shape was separated from chin. Distal segment was rotated to reduction side. Because of rotation of distal segment, space is made in opposite side. Seperated segement of wedge shape from large side is switched this space to fill. So, stability of distal segment is achieved. Authors applied to swiching genioplasty the patients who was remained the chin asymmetry after both sagittal split ramus osteotome was done because mandible asymmetry. After operation, patient and operator were satisfied with excellent esthetic results without any other complication. The switching genioplasty is effective surgical technique for chin asymmetry because it has more advantages than conventional sliding genioplasty. First, other donor side does not need for bone graft. Second, the switching genioplasty can reduce infection, bone resroption, dehiscence, capsular contraction after allograft. Third, have little mental nerve damage. Forth, anteroposterior correction is possible. Fifth, operation time is less than other genioplasty for chin asymmetry.
This study was designed to investigate the appearence and the characteristics of the apoptotic cells and the process of the joint cavity formation in mouse knee joint. Fetal mouse knee joints from 15 to 19 days of gestation were used. Paraffin-embedded serial sections, stained with H & E for light microscopic observation, Epon 812 embedded thin sections for electron microscopic observation and Lowicryl HM 20 embedded thin sections for immune-electron microscopic observation were prepared. Monoclonal antibodies to $\beta-tubulin$ and polyclonal antibodies to tissue transglutaminase were used for immune-electron microscopic study. The results obtained were as follows. 1. At 15 days of gestation, blood vessels, which have invaded in the mesenchymal cells, were present in the synovium, to form the joint cavity in the future. 2. At 16 days of gestation, the joint cleft was first appeared and several RBCs were present in the joint cleft. The invasion of blood vessels into the joint cleft was continuing, and apoptotic cells were present in the inner cell layer, adjacent to the joint cleft. Necrotic cells were also present in the outer cell layer; they were present 18 days of gestation, but apoptotic cells did not appear after 17 days of gestation. 3. In the apoptotic cells, transglutaminase were localized around vacuoles and the marginal site of the cytoplasm. 4. In the apoptotic cells, tubulin was around the endoplasmic reticulum and the marginal site of the cytoplasm. In the late stage of apoptotic cells, tubulin was localized diffusely in the cytoplasm. Tubulin was also strongly labeled around in the cytoplasm of the neighboring cell at which the apoptotic body was phagocytosed. Tubulin labeled particles were apparently increased in the seperated apoptotic bodies. On the basis of the above findings, it is proposed that during the development of the mouse knee joint, blood vessel invasion first occurs and then apoptosis and cell necrosis follow it. In the apoptotic cell, present in the synovium of the developing knee joint of the mouse. it is suggested that the redistribution of tubulin is associated with apoptotic process. And transglutaminase overexpressed in the apoptotic cell.
Porphyromonas endodontalis is a black-pigmented anaerobic Gram negative rod which is associated with endodontal infections. It has been isolated from infected dental root canals and submucous abscesses of endodontal origin. DNA probe is an available alternative, offering the direct detection of a specific microorganism. Nucleic-acid probes can be off different types: whole different: whole-genomic, cloned or oligonucleotide probes. Wholegenomic probes are the most sensitive because the entire genome is used for possible hybridization sites. However, as genetically similar species of bacteria are likely to be present in specimences, cross-reactions need to be considered. Cloned probes are isolated sequences of DNA that do not show cross-reactivity and are produced in quantity by cloning in a plasmid vector. Cloned probes can approach the sensitivity found with whole-genomic probes while avoiding known cross-reacting species. Porphyromonas endodontalis ATCC 35406 (serotype $O_1K_1$) was selected in this experiment to develop specific cloned DNA probes. EcoR I-digested genomic DNA fragments of P. endodontalis ATCC 35406 were cloned into pUC18 plasmid vector. From the E. coli transformed with the recombinant plasmid 4 clones were selected to be tested as specific DNA probes. Restriction-digested whole-genomic DNAs prepared from P. gingivalis 38(serotype a), W50(serotype b), A7A1-28(serotype C), P. intermedia 9336(serotype b), G8-9K-3(serotype C), P. endodontalis ATCC 35406(serotype $O_1K_1$), A. a Y4(serotype b), 75(serotype a), 67(serotype c), were each seperated on agarose gel electrophoresis, blotted on nylon membranes, and were hybridized with digoxigenin-dUTP labeled probe. The results were as follows: 1. Three clones of 1.6kb(probe e), 1.6kb(probe f), and 0.9kb(probe h) in size, were obtained. These clones were identified to be a part of the genomic DNA of P. endodontalis ATCC 35406 judging from their specific hybridization to the genomic DNA fragments of their own size on Southern blot. 2. The clones of 4.9kb(probe i) was identified to be a part of the genomic DNA of P. endodontalis ATCC 35406. but not to specific for itself. It was hybridized to P. gingivalis A7A1-28, P. intermedia G89K-3.
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