• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.2
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• pp.123-129
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• 2000

A high cell density culture system for the anchorage dependent CHO cells was developed based on the combination of in removal of ammonium ion and microcarrier culture system, and semi-fed-batch feeding of glucose and glutamine was employed to the developed culture system. The glass bead was selected as an optimum microcarrier in terms of cell growth. An ammonium ion selective zeolite, Phillipsite-Gismondine, was packed in a dialysis menium ion. The semi-fed-batch operation was employer to the novel culture system for the high density cell culture, and the results showed the cell growth was improved by 32% and tPA productivity by 250%.

• Proceedings of the Korean Society of Computer Information Conference
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• 2017.07a
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• pp.66-68
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• 2017

본 논문에서는 한화 109조 원을 돌파하는 전 세계 게임 시장에서 매출의 중요성에 대해 제시한다. 게임을 홍보할 수 있는 수단인 인터넷 방송과 게임 구매를 결합시킨 ESD 방식에 대한 조사 내용 및 분석한 결과도 제시한다. 이전에 존재하지 않았던 구매방식으로 사용자가 더욱 편리한 방법으로 게임을 구매하여 게임의 판매 수익을 증대시키고 인터넷 방송과 스트리머의 효율성을 높여 게임 개발사, 유통사, 스트리머, 사용자 모두 상부상조하여 부가가치까지 얻을 수 있는 방법도 함께 제시한다.

• Proceedings of the Korean Society of Computer Information Conference
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• 2015.07a
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• pp.238-239
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• 2015

본 논문에서는 국내 게임 시장 규모에서 다른 부분에 비해 떨어지는 콘솔 게임 시장의 발전방향으로 클라우드 기반의 콘솔게임기술에 대한 내용 제안한다. 콘솔게임의 새로운 패러다임의 방향은 사람들이 콘솔의 가장 큰 단점으로 꼽은 기기의 가격으로 인한 경제적인 부담을 덜어내며 부피의 간소화의 효과 또한 기대한다. 이러한 기대로 단점을 보완하여 콘솔 게임을 대중화 시킨다면 자연스레 시장의 확장으로 이어지며 이러한 시장 확장 상황에서 기업들의 정책 방향을 연구할 수 있을 것으로 기대한다.

• Korean Journal of Plant Resources
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• v.22 no.6
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• pp.518-521
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• 2009

High productivity of ginger (Zingiber officinale Roscoe) was obtained from the rhizome produced by shoot-tip culture with Korean native variety, Seosanjong. Seed rhizomes induced by shoot-tip culture were successfully established in the field. The rhizomes induced by both plant or rhizome were higher in emergence rate and faster in days to emergence than those of home seed production. The seed rhizome production induced by shoot-tip culture was two times heavier than that of home seed production. These results suggest that shoot-tip culture might be one of mass propagation methods in seed rhizome of ginger plant.

• The International Journal of Costume Culture
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• v.6 no.1
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• pp.52-60
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• 2003

The purpose of this study was to examine the cases of the head family's costume culture and to apply them to field experience education of adolescent's home economics subject. To examine costume culture, women in the two head families in the Hampyeong area, Jeollanam-do were interviewed. The findings had unique shroud making and custom in the Mo's head family of Hampyeong. Also it was found that the eldest daughters-in-law of the Lee's of Hampyeong have horizontally exchanged the information of the head family's costume with village women of the same family. They have initiated their costume culture directly through their daughters and it had the great influence on after-ages. The Spot experience program for adolescent to experience the head family's costume culture and life culture consisted of four subjects:(l)Educating the head family's traditional living culture including etiquette training, (2)head family village walk, (3)experiencing the head family's clothes life including natural dyeing and sewing, and (4)a field trip of Hampyeong Local Life Culture Museum. For a model experience of the head family's costume and life culture, three families with middle and high schoolers participated in 8- hour experience learning program.

• Preventive Nutrition and Food Science
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• v.2 no.1
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• pp.66-70
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• 1997

A mixed-culture of Gluconobacter suboxydans IFO 3172 and Saccharomyces uvarum IFO 0751 was per-formed in a synthetic medium. the optimal inculum ratio of G. suboydans and S. uvarum for mixed-culture fermentation was 150:1. The optimum pH, incubation temperature and aeration rate for mixed-culture fer- mentation were 5.0, 3$0^{\circ}C$ and 2.25vvm, reapectively. As a result of batch pure-and mixed-culture fer-mentation, specific growth rate in pure-culture of both strain was lower than that in mixed-culture. The yield of cell mass from S. uvarum exclusively decreased. The growth rate of the mixed-culture was very similar to the pure-culture in the begining of culture, but it has been decreased after 16hrs. In the mean time, S. uvarum in mixed-culture fermentation could grow due to fructose converted, but it could not row in pure-culture fermentation. Thus, the relationship was a sort of commensalism. The kinetic parameters cal-culated through steady-state results during continuous fermentations are as follows :{TEX}$$\mu$_{max1}${/TEX}=0.118({TEX}$h^{-1}${/TEX}), {TEX}$Ks_{1}${/TEX}=0.330(g/L),:{TEX}$$\mu$_{max2}${/TEX}=0.162({TEX}$h^{-1}${/TEX}), {TEX}$Ks_{2}${/TEX}=0.038(g/L). The yield of bacterial cell mass relatively constant, but yield of yest cell mass was gradually decreased.

• Journal of Fashion Business
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• v.14 no.5
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• pp.158-175
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• 2010

Culture means a lifestyle realizing a definite object or ideal. Each local special culture is enormous in value as a local culture inheritance. If it is developed a local culture products representing local culture, it can perform an important role on one of the strategies for revitalizing local economy. One of the typical cultures in Kyung-Nam is the Gaya culture. The most characteristic of the Gaya culture is powerful iron culture and lots of cultural properties have been founding as relics. Judging from a lot of iron relics, we can figure out a high level of iron manufacturing technology. I studied focussing on the plasticity of Gaya relics and collected base materials for developing local cultural goods, using the motif of Gaya culture with excellent aesthetic consciousness. I classfied Gaya relics into a crown style, jewelry, harnessry, weapons, armor, earthenware, and considered its characteristic of the plastic arts, based on the preceding studies and document data. There exists natural, moderate, polished, indigenous, simple, rhythmical, delicate, florid, technical, symbolical, strong, diverse, naive beauty in the plastic characteristic of Gaya relics. Gaya culture with the special excellence of aesthetic resources, is worthy enough to be recreated as local cultural goods. Variable and special cultural fashion-products with the distinctive feature of Gaya culture need to be developed without delay.

• Journal of Korean Foot and Ankle Society
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• v.18 no.2
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• pp.68-71
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• 2014

Purpose: The purpose of this study is to report the results of culture test at the time of removal of metal devices used for management of ankle fractures and for analysis of contributing factors. Materials and Methods: We reviewed medical records of 132 patients with lower tibia and ankle fracture who had their metal devices removed during the period from January 2010 to February 2014. Patients with clinical signs of infection were excluded. Culture test was performed by taking the granulation tissue around the metal device at the time of removal. We divided the subjects into two groups, culture positive and negative. We then performed a retrospective review of each medical record of multiple factors that might contribute to the culture results, including laboratory results, medical history, material and size of metal device, indwelling period, and whether or not it was open injury. Results: Among 132 cases, six were culture positive. Enterococcus was detected in two cases and the others were Staphylococcus. No significant difference in medical history of patients and laboratory results, including C-reactive protein level, was observed between the culture positive and negative group. Culture positive rate was 5.4% in titanium and 3.9% in stainless steel. In terms of metal size, culture positive rate was 5.1% in small plates, 6.7% in large plates, and culture negative in intramedullary nails. The average indwelling period of metal device was 61.5 weeks in the culture positive group, and 68.6 weeks in the negative group. Nine cases were open fractures and all were in the culture negative group. Conclusion: Whether or not the culture result was positive, there were no meaningful contributing factors. Presence of bacterium on the metal device could not be screened by any laboratory results or other factors.

• Journal of Microbiology and Biotechnology
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• v.11 no.2
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• pp.211-216
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• 2001

The growth of Selenastrum capricornutum in culture was investigated as a function of ionic copper concentrations to verify the toxicity and physiological variation. In a $Cu^{++}$ excess culture (E: $Cu^{++}$ of $130{\mu}g/l$), the growth rate (K)(0.32) was lower than that of the control culture (C: $Cu^{++}$ of $0.065{\mu}g/l$) (0.61) after 8 days and the exponential growth rate ($R_E$) was also found to be lower in culture E (1.1) than culture C (2.9). On the contrary, the K of S. capricornutum in trace $Cu^{++}$ culture ($T_1$ and $T_2$, 0.72) after 6 days tended to be more increased than culture C (0.68). From 8 to 14 days of culture, the amounts of chlorophyllls a and b were increased in culture C (chlorophyll a, $106->126{\mu}g/g$ dry wt; chlorophyll b, $158->208{\mu}g/g$ dry wt; chlorophyll b were decreased in culture E (chlorophyll a, $309->235{\mu}g/g$ dry wt; chlorophyll b, $405->352{\mu}g/g$ dry wt). The amounts of chlorophylls in ionic copper trace culture ($T_1$ and $T_2$) [(chl a/b) of $T_1$: $384/620\;{\mu}g/g$ dry wt; (chl a/b) of $T^2$:$320/467{\mu}g/g$ dry wt] were increased more than the culture C($260/387{\mu}g/g$ dry wt). Howeveer, when photosynthetic rates were normalized to the dry weight of algae, the control culture continued to showed higher values than the treated culture ($T_1$). An appropriate amount of ionic copper ($T_1:\;26\;{\mu}g/l$) stimulated the grwoth of S. capricornutum than the ionic copper content of $13\;{\mu}g/l$ ($T_2$), while the excess amount of ionic copper ($130\;{\mu}g/l$) resulted in the highest toxicity to the growth of S. capricornutum.

• Journal of Aquaculture
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• v.11 no.1
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• pp.91-97
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• 1998

This paper introduces to a simple culture method and growth of axenic (bacteria-free) rotifer Brachionus rotundiformis for seed stock of rotifer mass culture. This rotifer axenic culture method is based on the washing and transferring with sterilized sea water and modified antibiotic mixture AM9 solution. Population growth (final density on day 16) of axenic cultured rotifer were maintained with a high density and stable growth compared with the control of non-axenic culture (general culture style) through the 3 times-rerunning experiments (trial 1, 2 and 3). But the egg carrying rates of amictic females were not different between the axenic-and non-axenic culture condition. Although, rotifer density was higher in axenic culture, the food (Nannochloropsis oculata) was still remained unutilized than that of the non-axenic culture in third trial culture. These results suggest the possible existence of harmful bacteria for the rotifer population growth in the trial 1 and 3 of non-axenic culture compared to the trial 2. This axenic rotifer culture method is valuable for seed stock of the stable rotifer mass cultures.