• Journal of the Society of Naval Architects of Korea
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• v.46 no.6
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• pp.562-568
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• 2009

Hull form generation and variation methods to be mainly discussed in this study are based on the fairness optimized B-Spline form parameter curves (FOBFC). These curves can be used both as indirect modification function for variation and as geometric entities for hull form generation. The flexibility and functionality of geometric control technique play the most important role for the success of hull form optimization. This study shows the hydrodynamic optimization process and the characteristics of optimum design hull forms of a 14,000TEU containership and 60K LPG carrier. SHIPFLOW has been used as a CFD solver and FS-Framework as a geometric modeler and optimizer.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.9
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• pp.4267-4273
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• 2016

Previous studies suggested that dithio-carbamates are potent apoptosis and anti-apoptosis inducing agents in various cancer cells. Here, the anti-proliferative and apoptosis inducing effects of a new derivative (2-NDC) from the dithio-carbamate family was examined in human leukemia K562 cells. We use thiazolyl blue tetrazolium bromide (MTT) to measure viability and cell growth inhibition. The 2-NDC showed effects on viability in a dose and time-dependent manner, inhibiting proliferation at concentrations of $10-30{\mu}M$ after 24-48 hours of treatment and increasing values after 72 hours at $40-120{\mu}M$. The cytotoxic effect of the compound was calculated with an $IC_{50}$ of $30{\mu}M$ after 24-hour. Apoptosis induction was confirmed by acridine orange-ethidium bromide (AO/EtBr) staining, DNA fragmentation assay, flow cytometric assessment and also caspase-3 activation assay. Furthermore, enzymes level such as superoxide dismutase (SOD) and catalase (CAT) involved in oxidative stress were evaluated. The results of this study demonstrated insignificant increase of intracellular ROS levels for 24 hours and reduction after 48-72 hours. In addition to reduction of intracellular thiol, caspase-3 like activity was also decreased in a time-dependent manner in cells treated with 2-NDC. Thus 2-NDC can be considered as a good candidate for further pharmaceutical evaluations.

• BMB Reports
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• v.38 no.4
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• pp.391-398
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• 2005

3-hydrogenwadaphnin (3-HK) is a new daphnane-type diterpene ester isolated from Dendrostellera lessertii with strong anti-tumoral activity in animal models and in cultures. Here, prolonged effects of this new agent on proliferation and viability of several different cancerous cell lines were evaluated. Using [$^3H$]thymidine incorporation, it was found that the drug inhibited cell proliferation and induced G1/S cell cycle arrest in leukemic cells 24 h after a single dose treatment. The cell viability of Jurkat cells was also decreased by almost 10%, 31% and 40% after a single dose treatment (7.5 nM) at 24, 48 and 72 h, respectively. The drug-treated cells were stained with acridine orange/ethidium bromide to document the chromatin condensation and DNA fragmentation. These observations were further confirmed by detection of DNA laddering pattern in the agarose gel electrophoresis of the extracted DNA from the treated cells. Treatment of K562 cells with the drug at 7.5, 15 and 30 nM caused apoptosis in 25%, 45% and 65% of the cells, respectively. Exogenous addition of $25-50\;{\mu}M$ guanosine and/or deoxyguanosine to the cell culture of the drug-treated cells restored DNA synthesis, released cell arrest at G1/S checkpoint and decreased the apoptotic cell death caused by the drug. These observations were not made using adenosine. However, the drug effects on K562 cells were potentiated by hypoxanthine. Based on these observations, perturbation of GTP metabolism is considered as one of the main reasons for apoptotic cell death by 3-HK.

• Korean Journal of Food Science and Technology
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• v.36 no.5
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• pp.812-817
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• 2004

Cytotoxic effects of Korean rice-wine (Yakju) made with different processes and ingredients (Korean rice-wines I, II), red wine, white wine, beer, and Japanese rice-wine (Sake) were examined against human cancer lines (DLD-1, HepG2, K562) and mouse cancer lines (EMT6, LLC1). Red wine showed cytotoxic effect on all cancer lines, while Korean rice-wines I, and II showed cytotoxcity on all cancer cells except DLD-1. White wine, beer, and Japanese rice-wine had no or little cytotoxic effect against all cancer cell lines. Concentrate of Korean rice-wine only showed cytotoxic effect against DLD-1. These results suggest Korean rice-wine has strong anti-cancer effects, which are induced by certain rice-wine components.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.22 no.3
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• pp.562-568
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• 1998

During the past three decades, several techniques have been suggested for robust performance design of multivariable systems within the framework of Quantitative Feedback Theory. They are all characterized and limited by the use of loop transmission inversion. A new approach within framework is described which leads to a design tehnique without loop transmission inversion. Complete sequential design algorithms are derived for performance specifications in terms of plant input disturbance, sensitivity, complimentary sensitivity and control effort.

• Journal of the Korean Society of Clothing and Textiles
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• v.36 no.5
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• pp.562-572
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• 2012

Market mavens are known as powerful consumers for the diffusion of market information in general fields. This study investigates the market mavens in fashion consumers. Data were gathered by surveying university students in Seoul, using convenience sampling; 312 questionnaires were used in the statistical analysis. This study proved that consumers recognized the existence of market mavens and the importance of market mavens' information and their evaluations. In regards to the demographic factors, the more clothing expenditures and family income then the higher the tendencies of the market maven. Market mavens were consumers who were aware of marketer's persuasion but showed no differences in marketplace interfaces. Market mavens were particularly interested in dressing styles and were highly convinced in their abilities to make fashion decisions.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.246-246
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• 1994

연구목적: 생약으로 부터 항암제 개발연구의 일환으로 민간에서 조경. 지혈 등에 사용되고있는 노루발(Pyrola japonica)의 세포독성 물질과 그의 화학적구조를 규명하기 위해서 본 연구를 실시하였다. 실험 방법 : 옴건한 노루발의 지하부를 methanol로 환류 추출하여 얻은 methanol extract를 n-hexane, ethylacetate, n-butanol 순으로 분획하였다. 그 후 각각의 분획을 L1210 세포에 대하여 in vitro에서 실험한 결과 n-heane, ethylacetate 분획에서 강한 활성이 나타남을 알 수 있었다. 따라서 n-hexane, ethylacetate 분획을 반복하여 silica gel column chromatography 하여 1종의 활성물질과 4종의 비활성물질을 단리하였다. 실험결과 : 단리된 활성물질은 각종 spectral data를 검토하여 chimaphilin (L1210의 ED$_{50}$ : 0.6 $\mu\textrm{g}$/ml, K562의 ED$_{50}$ :< 0.6 $\mu\textrm{g}$/ml) 임을 확인하였고 이 물질은 5,8-dihydro-2.7-dimethyl-4-naphtoquione의 변성물질 임을 알 수 있었다.있었다.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.207-207
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• 1994

I) 지금까지 만성골수성백혈병의 치료법으로서 골수이식이나 인터페론의 투여등이 시행되어왔으며 백혈병세포에서 Interleukin-1$\beta$(이하 IL-1$\beta$)이 자율적으로 생성됨이 보고 되어 이러한 질병의 진행에 IL-1$\beta$의 활성증가가 관련될것이라는 견해가 대두되어 왔다. 최근 단핵구성백혈병 환자의 소변에서 분리된 IL-1수용체 길항제(1L-1 receptor antagonist: IL-lRA)가 clonig되었고 인터페론치료에 저항하는 환자들의 치료에 IL-1RA 가 이용될수 있을것이라는 견해가 있다. 따라서 본 연구는 유전공학연구소가 분리 정제한 IL-1RA가 만성골수백혈병 환자로부터 유래한 K562세포주의 증식에 미치는 영향을 평가하고 알파인터페론과의 병용투여시의 상호작용에 관해 검토하였다.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.21 no.4
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• pp.562-569
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• 1997

The axial position of an impeller is misaligned in the process of manufacturing and assembling. For a single suction centrifugal pump with balancing holes, the effect of axial displacement of impeller on the performance, leakage loss and axial thrust acting on the impeller is experimentally investigated. The axial displacement decreases the pump efficiency, increases the leakage through the clearance between wearing ring and impeller, and affects the characteristics of axial thrust.

• Proceedings of the Korea Information Processing Society Conference
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• 2016.10a
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• pp.562-565
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• 2016

첨단 과학 장비를 이용한 시뮬레이션의 결과로 데이터의 정확도 및 정밀도가 향상되어 대용량의 이차원 배열 데이터가 생성되고 있다. 대용량의 이차원 배열 데이터에서 유사 구역 (similar region)을 찾아내는 것은 매우 의미 있는 일이다. 따라서 본 논문에서는 대용량의 이차원 배열데이터에서 유사 구역을 찾는 단순 방법(naïve method)과 효율적으로 탐색할 수 있는 알고리즘을 제안한다. 또한 단순 방법과 제안 알고리즘의 시간 복잡도(time complexity)를 분석하고 실험을 통해 제안 방법이 단순 방법보다 더 빠르게 처리함을 보인다.