• Title/Summary/Keyword: K strain

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Isolation and Characterization of a New Fluorescent Pseudomonas Strain that Produces Both Phenazine 1-Carboxylic Acid and Pyoluteorin

  • HU, HONG-BO;XU, YU-QUAN;FENG CHEN;XUE HONG ZHANG;HUR, BYUNG-KI
    • Journal of Microbiology and Biotechnology
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    • v.15 no.1
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    • pp.86-90
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    • 2005
  • Strain M-18 was isolated from the rhizosphere soil of sweet melon, using 1-aminocyclopropane-1-carboxylate (ACC) as a sole nitrogen source. Its phenotypic characteristics, metabolic tests, and 16S rDNA sequence were analyzed. The antibiotics secreted by strain M-18 were determined to be phenazine 1-carboxylic acid and pyoluteorin. These data showed that strain M-18 was a new fluorescent Pseudomonas strain that produced both phenazine 1-carboxylic acid and pyoluteorin, some features being similar to Pseudomonas aeruginosa and Pseudomonas fluorescens. Therefore, the strain M-18 appears to be the first pseudomonad described to date that is capable of producing both phenazine 1-carboxylic acid and pyoluteorin.

High Speed Tensile Tests of Steel Sheets for an Auto-body at the Intermediate Strain Rate (중변형률 속도에서의 차체용 강판의 고속 인장실험)

  • Lim, Ji-Ho;Kim, Seok-Bong;Kim, Jin-Sung;Huh, Hoon;Lim, Jong-Dae;Park, Sung-Ho
    • Transactions of the Korean Society of Automotive Engineers
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    • v.13 no.2
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    • pp.127-134
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    • 2005
  • This paper introduces a newly developed high speed material testing apparatus for tensile tests at the strain rate up to 500/sec. The tensile properties of sheet metals are indispensable for the accurate crashworthiness analysis of auto-bodies since the local strain rate reaches to 500/sec in the car crash. An appropriate experimental method has to be developed to acquire the tensile properties at the intermediate strain rate ranged from 0.003/sec to 200/sec. Tensile tests of various different steel sheets for an auto-body were perform ed to obtain the dynamic properties with respect to the strain rate. The dimensions of specimens that can provide the reasonable results were determined by the finite element analysis. A special jig fixture of a load cell is designed to reduce the load ringing phenomenon induced by unstable stress propagation at the high strain rate. Stress-strain curves were acquired for each steel sheet from the dynamic tensile test and utilized to obtain the relationship of the stress to the strain rate.

Identification of a Strain of Babesia Isolated from Korean Cattle (한우(韓牛)로부터 분리(分離)한 바베시아 원충(原蟲)의 동정(同定))

  • Jeon, Yeong
    • Korean Journal of Veterinary Research
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    • v.18 no.1
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    • pp.27-31
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    • 1978
  • In order to identify unknown Babesia spp. which was isolated from Korean cattle, the morphology of Korean strain was compared with that of Babesia bigemina (Kochinda strain) and Babesia spp. (Miyake strain). Immunofluorescent technique was used to identify the serological character of the parasites. The results obtained were summarized as follows: 1. Korean strain was morphologically very similar to Babesia spp.(Miyake strain) which mostly showed parallel-bigeminate forms, while B. bigemina (Kochinda strain) was mostly round and oval forms. 2. By the indirect fluorescent antibody technique: a) Anti-Babesia spp. and Korean Babesia spp. sera showed a higher antibody titers with Babesia spp. (Miyake strain) antigen (1:500) than with B. bigemina (Kochinda strain) antigen (1:50). b) Anti-Babesia bigemina sera showed a lower titer with Babesia spp. antigen (1:50) than with B. bigemina antigen (1:250). 3. On the basis of morphological and serological confirmantions, a Babesia strain isolated from a Korean cattle was very similar, if not identical, to Miyake strain of Babesia spp.

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A Study on the Evaluation of the Automatic Measurement Method of Plastic Strain Ratio by Two Extensometers (신장계에 의한 소성변형비 자동측정법의 평가에 관한 연구)

  • 김인수
    • Transactions of Materials Processing
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    • v.12 no.5
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    • pp.504-512
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    • 2003
  • The plastic strain ratios(R-values) of low carbon steel sheets were determined by the automatic strain measurement method using two extensometers, the indirect photo method for the same tensile specimen during tensile test and the indirect method for the specimen after tensile test. The experimental results showed that the measured plastic strain ratios from the automatic strain measurement method using two extensometers coincided with those from the indirect photo method and the indirect method for all tensile specimens. In addition, the strain dependence of plastic strain ratios could be continuously recorded and the anisotropy of the strength coefficient, K, and strain hardening exponent, n, could be automatically calculated in three directions by computer through the use of two extensometers. The experimental results showed that the strain dependence of R-value was related to the anisotropy of strain hardening exponent in low carbon steel sheets.

Characterization of Bacillus thuringiensis StrainBT-14 having Insecticidal Activity against Plutella xylostella

  • Bok, Song-Hae-Jung, Yong-Chul;Kim, Sung-Uk;Son, Kwang-Hee;Lee, Hyung-Hoan
    • Journal of Microbiology and Biotechnology
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    • v.4 no.4
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    • pp.322-326
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    • 1994
  • Bacillus thuringiensis strain BT-14 was isolated from alfalfa dust in Korea. The strain BT-14 produced one bipyramidal crystal and one spore in the cell. The biochemical characteristics of the strain BT-14 were similar to those of Bacillus thuringiensis subsp. kurstaki HD-l. Examination of its antibiotic resistance revealed that while the strain BT-14 was less resistant than BTK HD-l to ampicillin, gentamycin, neomycin and tobramycin, it was more resistant to amikacin than BTK HD-l. The $\delta$-endotoxin crystal of strain BT-14 consisted of a single protein with a high molecular weight of ca 135 KD on a 10% SDS-PACE. The strain BT-14 contained at least nine different plasmids with sizes of 2.9, 5.3, 5.8, 6.2, 9.4, 15.1, 18.1, 23.1 and 79 Kb. In insect bioassay, the isolated strain BT-14 showed lethality of 67% against Plutella xylostella larvae at dilution of 5$\times$$l0^{-4}$ (5$\times$l0 to 3$\times$$l0^2$ spores/ml), which is, almost equivalent to that of BTK HD-l.

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Restrained Effect of End Plate on Plane Strain Test Evaluated by Digital Image Correlation Method (디지털 이미지 코릴레이션 기법으로 평가한 평면변형률 시험의 단부 구속 효과)

  • Jang, Eui-Ryong;Choo, Yoon-Sik;Lee, Won-Taeg;Chung, Choong-Ki
    • Proceedings of the Korean Geotechical Society Conference
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    • 2008.03a
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    • pp.22-33
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    • 2008
  • The plane strain test has been used widely in order to examine the stress-strain relation and failure behavior. Its advantages are more realistic simulation of deformation and failure behaviors of soils. Most plane strain tests have been carried out with restrained end plates due to difficulties in manufacturing the equipment with free end condition and also performing it. In this study, plane strain tests with/without bottom plate restraint were performed on Jumunjin-sand. The measurement of overall and local deformation was accomplished by digital image correlation technique as well as external LVDT. By applying digital image correlation method using two consecutive images captured through the transparent wall, local deformation behavior of various parts inside the specimen was estimated. From digital image analysis result, the restrained effect of end plate was examined about formation and development of shear band, and deformation mechanism of sand under plane strain condition.

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Interpretation of Limit Creep Strain of Geogrids by Sherby-Dorm Plots (Sherby-Dorm Plots에 의한 지오그리드의 한계크리프변형률 해석)

  • Jeon, Han-Yong;Mok, Mun-Sung;Jin, Yong-Bum;Lim, Ji-Hye
    • Proceedings of the Korean Geotechical Society Conference
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    • 2005.03a
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    • pp.1572-1579
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    • 2005
  • New procedure for evaluation of creep reduction factor using performance limit strain concept was introduced and confirmed through the creep test results. To determine the performance limit strain of the textile geogrid used in this study, the Sherby-Dorm Plots were applied and the results were compared with the results that applied existed limit strain criteria (GRI test method GG-4). The limit creep strain of the geogrid samples that determined by using the Sherby-Dorm Plots were all 11%. This value is more higher than the existed criteria as 10%. From this 11% limit strain the creep reduction factors were calculated at 100,000 hours design. It was resulted in 1.45 for all of the geogrid samples(8t/m, 10t/m). Finally, when it was compared with the creep reduction factors that using 10% criteria, there were some decrease of reduction factor values about $0.06{\sim}0.14$.

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In situ reduction of gold nanoparticles in PDMS matrices and applications for large strain sensing

  • Ryu, Donghyeon;Loh, Kenneth J.;Ireland, Robert;Karimzada, Mohammad;Yaghmaie, Frank;Gusman, Andrea M.
    • Smart Structures and Systems
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    • v.8 no.5
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    • pp.471-486
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    • 2011
  • Various types of strain sensors have been developed and widely used in the field for monitoring the mechanical deformation of structures. However, conventional strain sensors are not suited for measuring large strains associated with impact damage and local crack propagation. In addition, strain sensors are resistive-type transducers, which mean that the sensors require an external electrical or power source. In this study, a gold nanoparticle (GNP)-based polymer composite is proposed for large strain sensing. Fabrication of the composites relies on a novel and simple in situ GNP reduction technique that is performed directly within the elastomeric poly(dimethyl siloxane) (PDMS) matrix. First, the reducing and stabilizing capacities of PDMS constituents and mixtures are evaluated via visual observation, ultraviolet-visible (UV-Vis) spectroscopy, and transmission electron microscopy. The large strain sensing capacity of the GNP-PDMS thin film is then validated by correlating changes in thin film optical properties (e.g., maximum UV-Vis light absorption) with applied tensile strains. Also, the composite's strain sensing performance (e.g., sensitivity and sensing range) is also characterized with respect to gold chloride concentrations within the PDMS mixture.

An efficient method for the compressive behavior of FRP-confined concrete cylinders

  • Fan, Xinglang;Wu, Zhimin;Wu, Yufei;Zheng, Jianjun
    • Computers and Concrete
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    • v.12 no.4
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    • pp.499-518
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    • 2013
  • Fiber reinforced polymer (FRP) jackets have been widely used as an effective tool for the strengthening and rehabilitation of concrete structures, especially damaged concrete columns. Therefore, a clear understanding of the compressive behavior of FRP-confined concrete is essential. The objective of this paper is to develop a simple efficient method for predicting the compressive strength, the axial strain at the peak stress, and the stress-strain relationship of FRP-confined concrete. In this method, a compressive strength model is established based on Jefferson's failure surface. With the proposed strength model, the strength of FRP-confined concrete can be estimated more precisely. The axial strain at the peak stress is then evaluated using a damage-based formula. Finally, a modified stress-strain relationship is derived based on Lam and Teng's model. The validity of the proposed compressive strength and strain models and the modified stress-strain relationship is verified with a wide range of experimental results collected from the research literature and obtained from the self-conducted test. It can be concluded that, as a competitive alternative, the proposed method can be used to predict the compressive behavior of FRP-confined concrete with reasonable accuracy.

Fatigue Life Evaluation of Spot Weldments of SPC Sheet Including Strain Rate Effect (변형률속도효과를 고려한 일반냉연강판 점용접부의 피로수명평가)

  • Song, Joon-Hyuk;Nah, Seok-Chan;Yu, Hyo-Sun;Kang, Hee-Yong;Yang, Sung-Mo
    • Transactions of the Korean Society of Automotive Engineers
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    • v.14 no.1
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    • pp.48-53
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    • 2006
  • A methodology is described for predicting the fatigue life of the resistance spot weldment including strain rate effect. Because it is difficult to perform a physical failure test with high strain rate, an analytical method is necessary to get the mechanical properties of various strain rate, To this end, quasi-static tensile-shear tests at several strain rate were performed on spot weldments of SPC. These test provided the empirical data with the strain rate. With these results, we formulated the function of fatigue life prediction using the lethargy coefficient which is the global material property from tensile test. And, we predicted the fatigue life of spot weldment at dynamic strain rate. To confirm this method for fatigue life prediction, analytical results were compared with the experimental fatigue data.