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Isolation and Characterization of a New Fluorescent Pseudomonas Strain that Produces Both Phenazine 1-Carboxylic Acid and Pyoluteorin  

HU, HONG-BO (College of Life Science and Biotechnology, Shanghai Jiaotong University)
XU, YU-QUAN (College of Life Science and Biotechnology, Shanghai Jiaotong University)
FENG CHEN (College of Life Science and Biotechnology, Shanghai Jiaotong University)
XUE HONG ZHANG (College of Life Science and Biotechnology, Shanghai Jiaotong University)
HUR, BYUNG-KI (Department of Biological Engineering, Inha University)
Publication Information
Journal of Microbiology and Biotechnology / v.15, no.1, 2005 , pp. 86-90 More about this Journal
Abstract
Strain M-18 was isolated from the rhizosphere soil of sweet melon, using 1-aminocyclopropane-1-carboxylate (ACC) as a sole nitrogen source. Its phenotypic characteristics, metabolic tests, and 16S rDNA sequence were analyzed. The antibiotics secreted by strain M-18 were determined to be phenazine 1-carboxylic acid and pyoluteorin. These data showed that strain M-18 was a new fluorescent Pseudomonas strain that produced both phenazine 1-carboxylic acid and pyoluteorin, some features being similar to Pseudomonas aeruginosa and Pseudomonas fluorescens. Therefore, the strain M-18 appears to be the first pseudomonad described to date that is capable of producing both phenazine 1-carboxylic acid and pyoluteorin.
Keywords
Biocontrol; phenazine 1-carboxylic acid; Pseudomonas aeruginosa; Pseudomonas fluorescens; pyoluteorin;
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1 Bergey, D., G. H. John, R. K. Noel, and H. A. S. Peter. 1994. Bergey's Manual of Determinative Bacteriology. 9th Ed. Lippincott Williams & Wilkins, Philadelphia, U.S.A.
2 King, E. O., M. K. Ward, and D. E. Raney. 1954. Two simple media for the demonstration of pyocyanine and fluorescein. J. Lab. Clin. Med. 44: 301- 307   PUBMED
3 Lee, J. Y., S. S. Moon, and B. K. Hwang. 2003. Isolation and in vitro and in vivo activity against Phytophthora capsid and Colletotrichum orhiculare of phenazine-l-carboxylic acid from Pseudomonas aeruginosa strain GC-B26. Pest Manag. Sci. 59: 872- 882   DOI   ScienceOn
4 Levitch, M. E. and E. R. Stadtman. 1964. A study of the biosynthesis of phenazine-l-carboxylic acid. Arch. Biochem. Biophy. 106: 194- 199   DOI   ScienceOn
5 Nowak-Thompson, B., S. J. Gould, J. Kraus, and J. E. Loper. 1994. Production of 2,4-diacetylphloroglucinol by the biocontrol agent Pseudomonasfluorescens Pf-5. Can. J. Microbiol. 40: 1064- 1166   DOI   ScienceOn
6 Raaijmakers, J. M., M. Vlami, and J. T. Souza. 2002. Antibiotic production by bacterial biocontrol agents. Antonie van Leeuwenhoek 81: 537- 547   DOI   ScienceOn
7 Stover, C,K., X. Q. Pham, A. L. Erwin, S. D. Mizoguchi, P. Warrener, and M. J Hickey, et al. 2000. Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature 406: 959-964   DOI   ScienceOn
8 Pierson III, L. S., G. Thomas, L. Stephen, and F. Gong. 1995. Molecular analysis of genes encoding phenazine biosynthesis in the biological control bacterium Pseudomonas aureofaciens 30-84. FEMS Microbiology Lett. 134: 299- 307   DOI   PUBMED
9 Bender, C. L., V. Rangaswamy, and J. Loper. 1999. Polyketide production by plant-associated Pseudomonads. Annu. Rev. Phytopathol. 37: 175-196   DOI   ScienceOn
10 Haas, D., C. Blumer, and C. Keel. 2000. Biocontrol ability of fluorescent pseudo monads genetically dissected: Importance of positive feedback regulation. Curr. Opin. Biotechnol. 11: 290- 297   DOI   ScienceOn
11 Duffy, B. K. and G. Defago. 1999. Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas Fluorescens biocontrol strains. Appl. Environ. Microbiol. 65: 2429- 2438   PUBMED
12 Slininger, P. J. and M. A. Shea-Wilbur. 1995. Liquid-culture pH, temperature, and carbon (not nitrogen) source regulate phenazine productivity of the take-all biocontrol agent Pseudomonasfluorescens 2-79. Appl. Microbiol. Biotechnol. 43: 794- 800   DOI   ScienceOn
13 Lanyi, B. 1987. Classical and rapid identification methods for medically important bacteria. Methods Microbiol. 19: 167
14 Huang, X. Q., D. H. Zhu, Y. H. Ge, H. B. Hu, X. H. Zhang, and Y. Q. Xu. 2004. Identification and characterization of pltZ, a gene involved in the repression of pyoluteorin biosynthesis in Pseudomonas sp M18. FEMS Microbiol. Lett. 232(2): 197-202   DOI   ScienceOn
15 Nowak-Thompson, B., N. Chaney, J. S. Wing, S. J. Gould, and J. E. Loper. 1999. Characterization of the pyoluteorin biosynthetic gene cluster of Pseudomonas fluorescens Pf-5. J. Bacteriol. 181: 2166-2174   PUBMED
16 Kreig, N. R. and J. G. Holt. 1989. Gram negative rods and cocci. In: Bergey's Manual of Systematic Bacteriology, Williams & Wilkins. Baltimore, U.S.A.
17 Slininger, P. J., J. E. Van Cauwenberge, R. J. Bothast, D. M. Weller, L. S. Thomashow, and R. J. Cook. 1996. Effect of growth culture physiological state, metabolites, and formulation on the viability, phytotoxicity, and efficacy of the take-all biocontrol agent Pseudomonas fluorescens 2-79 stored encapsulated on wheat seeds. Appl. Microbiol. Biotechnol. 45: 391- 398   DOI   ScienceOn
18 Keen, N. T., S. Tarnaki, D. Kobayashi, and D. Trollinger. 1988. Improved broad-host-range plasm ids for DNA cloning in gram-negative bacteria. Gene 70: 191- 197   DOI   ScienceOn
19 Shaukat, S. S. and I. A. Siddiqui. 2003. The influence of mineral and carbon source on biological control of charcoal rot fungus, Macrophomina phaseolina by fluorescent pseudomonads in tomato. Lett. Appl. Microbiol. 36: 392-398   DOI   ScienceOn
20 Glick, S. R., D. M. Karaturovic, and P. C. Newell. 1995. A novel procedure for rapid isolation of plant growth promoting pseudomonads. Can. J. Microbiol. 41: 533- 536   DOI   ScienceOn
21 Sambrook, J., E. F. Fritsch, and A. E. Roodbeen. 1989. Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor, Cold Spring Harbor Laboratory Press, New York, U.S.A.
22 Thomashow, L. S. 1996. Biological control of plant root pathogens. Curr. Opin. Biotechnol. 7: 343- 347   DOI   PUBMED   ScienceOn
23 Mavrodi, D. V., V. N. Ksenzenko, R. F. Bonsall, R. J. Cook, A. M. Boronin, and L. S. Thomashow. 1998. A sevengene locus for synthesisof phenazine-l-carboxylic acid by Pseudomonas fluorescens 2-79. J. Bacteriol. 180: 2541-2548   PUBMED