• Title/Summary/Keyword: K

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Partial Characterization and Purification of Enterocin K25 Linked to the Plasmid in Enterococcus sp. K25

  • Moon, Gi-Seong;Kim, Wang-June
    • Food Science and Biotechnology
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    • v.14 no.5
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    • pp.581-585
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    • 2005
  • The antimicrobial activity of partially purified enterocin K25, produced by Enterococcus sp. K25, was abolished by proteases such as pepsin and proteinase K. The bacteriocin was resistant to heat treatment at $75^{\circ}C$ for 15 min and lost 75% of its activity at $100^{\circ}C$ for 30 min. Enterocin K25 showed bactericidal mode of action against an indicator strain, Lactobacillus plantarum NCDO 955. Enterocin K25 was purified to 112.6-fold purity via conventional steps of ammonium sulfate precipitation, ion exchange chromatography, and reversed phase high performance liquid chromatography (RP-HPLC). The molecular mass of the purified enterocin K25 was estimated as 4.3 kDa on an electrophoresis gel. Plasmid (${\sim}6.5\;kb$) linkage of production of enterocin K25 was confirmed by plasmid curing.

Thermococcus onnurineus NA1: a Lithoheterotroph as Revealed by Genome Analysis

  • Lee, Hyun-Sook;Kang, Sung-Gyun;Bae, Seung-Seob;Lim, Jae-Kyu;Cho, Yon-A;Kim, Yun-Jae;Jeon, Jeong-Ho;Cha, Sun-Shin;Kwon, Kae-Kyoung;Kim, Hyung-Tae;Park, Cheol-Joo;Lee, Hee-Wook;Kim, Seung-Il;Chun, Jong-Sik;Colwell, Rita R.;Kim, Sang-Jin;Lee, Jung-Hyun
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2008.05a
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    • pp.119-120
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    • 2008
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The Enhancement of Immunological Activity by Mild Hypothermia

  • Hasegawa Takeo;Gu Yeunhwa;Miyata Katuyuki;Maeda Kayoko;Fukuyama Atsushi;Ando Satoshi;Amano Morikazu;Suzuki Tomoaki;Murabayashi Kousuke;Ida Kazushi;Yukiko Ohno;Kitaoka Hitomi;Kureki Michihiro
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2004.11a
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    • pp.205-209
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    • 2004
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Extending the Password-based Authentication Protocol K1P (패스워드 기반 인증 프로토콜 K1P의 확장)

  • 권태경;송주석
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.23 no.7
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    • pp.1851-1859
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    • 1998
  • We summarize the password-based authetication protocol K1P which was introduced in our easlier papers [1,2] and then propose three more extended protocols. These protocols preserve a design concept of K1P, i.e., security and efficiency, and canbe used for various purposes. They are a One-time key K1P, a Client public key K1P, and an Exponential key exchange K1P.

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16-ns 256K CMOS SRAM (16-ns 256k CMOS SRAM)

  • Kim, B.Y.;Jung, T.S.;Park, H.C.;Hwang, S.K.;Park, Y.B.;Kim, C.R.;Choi, K.H.
    • Proceedings of the KIEE Conference
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    • 1988.07a
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    • pp.311-314
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    • 1988
  • This paper describes 256k (256K ${\times}$1) CMOS SRAM utilizing 1.2um double-polysilicon and double-metal CMOS process. A typical access time of 16ns with a 30-pF load has been achieved through the use of a block architecture, a new decoder, an unique bit-line scheme and an optimized process. Operating current is 55mA at 40MHz and 15mA at 10MHz. A high-resistive polysilicon load has been used to achieve a standby current of 3uA.

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