• Title/Summary/Keyword: K

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Prevalence of Fimbrial Antigen (K88 variants, K99 and 987P) of Enterotoxigenic Escherichia Coli from Neonatal and Post-weaning Piglets with Diarrhea in Central China

  • Wang, J.;Jiang, S.W.;Chen, X.H.;Liu, Z.L.;Peng, J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.9
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    • pp.1342-1346
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    • 2006
  • Enterotoxigenic Escherichia coli is a major cause of diarrhea in neonatal and post-weaning piglets. To determine the most common fimbrial antigens of ETEC in piglets with diarrhea, two investigations were carried out on intensive pig farms in Hubei province, central China. In 2002-2003, 227 fecal samples from neonatal and post-weaning piglets with diarrhea were tested for the presence of the fimbrial antigen K88 and K99 of ETEC by the polymerase chain reaction (PCR). Twenty-three (10.1%) of 227 fecal samples were found to contain fimbrial antigen K88, which was identified as K88ac variant; and 13 (5.7%) samples containing K99. In 2004, another 179 fecal samples from diarrheic piglets, 1 day to 6 weeks of age, were tested for prevalence of fimbrial antigen K88, K99 and 987P. Forty-seven (26.3%) of the 179 samples carried at least one of the ETEC fimbrial antigens. K88 antigen was detected in 20.1%. In the 36 samples known to carry fimbrial antigen K88, 32 (88.9%) contained K88ad; and 4 (11.1%) contained K88ac; none of them carried K88ab. Fimbrial antigens K99 and 987P were detected in 1.1% and 6.1%, respectively. Our data indicate that K88 is the most common fimbrial antigen of ETEC associated with diarrhea in piglets in Central China.

Two-dimensionally Integrated Fluorescent Lamp for 40 inch LCD-TV Application

  • Kim, Joong-Hyun;Hwang, In-Sun;Byun, Jin-Seob;Park, Hae-Il;Kim, Hyoung-Joo;Jang, Hyeon-Yong;Kang, Seock-Hwan;Kim, Min-Gyu;Kwon, Nam-Ok;Lee, Sang-Yu;Souk, Jun-Hyung;Ko, Jae-Hyeon;Lee, Ki-Yeon;Jung, Kyeong-Taek;Kim, Dong-Woo;Ha, Hae-Soo;Heon, Min;Kim, Nam-Hun;Kim, Hyun-Sook;Kim, Geun-Young;Cho, Seog-Hyun
    • 한국정보디스플레이학회:학술대회논문집
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    • 2004.08a
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    • pp.795-798
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    • 2004
  • After showing 32 inch two-dimensionally integrated fluorescent lamp (TIFL) and its module at SID '04, 40 inch TIFL and its module of prototype have been developed at the first time. It is the biggest size in the world as well as has a backlight unit without BEF optical film. The luminance of TIFL is 14000 nit at 190 watt power consumption and its luminous efficacy is 51 lumen/watt. The use of TIFL simplifies backlight assembly process and removes high price optical sheets. As a result, LCD TV, used by TIFL, is rapidly going to expand its market share in the large size TV area.

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E1B-19k does not Localize in Mitochondria nor Dimerize Bax even with the Staurosporine (E1B-19k의 세포내 위치와 Bax와의 Dimerization에 관한 연구)

  • Yoon, Soo Han;Kim, Jin Young;Park, Seung Woo;Ahn, Young Hwan;Ahn, Young Min;Cho, Ki Hong;Cho, Kyung Gi
    • Journal of Korean Neurosurgical Society
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    • v.29 no.6
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    • pp.725-730
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    • 2000
  • Purpose : The subcellular localization of E1B-19k has been known cytosol or nuclear membrane by immunohistochemical staining and could dimerize with Bax to regulate cell death also known by the in-vitro immunoprecipitation. We planed to confirm this dimerization of E1B-19k with Bax in vivo in Cos-7 cells by using green fluorescent protein. Material and Method : We cloned E1B-19k and Bax into C3-EGFP. C3-EGFP-E1B-19k, C3-EGFP-Bax, and C3-EGFP-E1B-19k and pcDNA3-Bax were transfected into Cos-7 cells. We explored location of E1B-19k and Bax, and confirmed its dimerization with Bax in transfected living healthy Cos-7 cells by following green fluorescent protein of E1B-19k on the confocal microscope. Results : E1B-19k was located diffusely in cytoplasm and in nucleus but not in mitochondria. It prevented cell death from the apoptosis by staurosporine but its location was not changed. GFP-E1B-19k is not changed its intracellular location with Bax even with staurosporine. Conclusion : These results support that E1B-19k does not localize in mitochondria nor dimerize with Bax even with staurosporine. We could anticipate E1B-19k prevent cell death via the other dimerizing partner or pathways.

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Introduction on the Contamination Design Standard(Revision Proposal) of 345kV/154kV Transmission System in KEPCO (345kV/154kV 직접접지계 송전설비의 내오손 설계기준(안) 해설)

  • Shim, E.B.;Woo, J.W.;Kwak, J.S.;Min, B.W.;Lee, O.B.;Shin, T.W.;Park, Y.S.
    • Proceedings of the KIEE Conference
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    • 2002.07a
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    • pp.262-264
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    • 2002
  • This paper shows the design standard of KEPCO on the contamination design for 345 kV and 154 kV transmission tower. Up to now, because the design standard of KEPCO on the insulation design contains 154 kV transmission system only, we had investigated the 343 kV system for the revision of design standard, with respect to the contamination design, we have used the same design philosophy which were adopted to the 765 kV transmission tower. In order to determine the number of insulator discs, we had investigated the withstand voltage of discs according to the level of ESDD(Equivalent Salt Deposit Density) and kinds of disc types. The TOV(Power Frequency Temporary Overvoltage) were estimated by EMTP(Electromagnetic Transient Program) for both 154 kV and 345 kV transmission system. The overvoltage level was appeared 1.35 p.u. between phase to ground for both 154 kV and 345 kV transmission system, but finally this factor was not applied to the design standard considering current design standard and economic point of view. With regard to classification of contamination area of 345 kV transmission system, we added the clean area which was not specified in the current design practise.

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A fluorogenic method for measuring enteropeptidase activity: spectral shift in the emission of GD4K-conjugated 7-amino-4-methylcoumarin

  • Choi, Mal-Gi;Lee, Eung-Yeong;Chung, Hye-Shin;Jang, Sei-Heon;Lee, Chang-Woo
    • BMB Reports
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    • v.44 no.7
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    • pp.458-461
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    • 2011
  • Enteropeptidase is a serine protease secreted by the pancreas and converts inactive trypsinogen to active trypsin. Enteropeptidase cleaves the C-terminal end of the substrate recognition sequence Asp-Asp-Asp-Asp-Lys ($D_4K$). The assay for enteropeptidase has utilized $GD_4K$-conjugated 2-naphthylamine ($GD_4K$-NA) as a fluorogenic probe over the last 30 years. However, no other $D_4K$-conjugated fluorogenic substrates of enteropeptidase have been reported. Furthermore, naphthalene is known as carcinogenic to humans. In this study, we used shift in the emission spectrum of $GD_4K$-conjugated 7-amino-4-methylcoumarin ($GD_4K$-AMC) as a fluorogenic method to measure enteropeptidase activity. The kinetic analysis revealed that enteropeptidase has a $K_M$ of 0.025 mM and a $k_{cat}$ of 65 $sec^{-1}$ for $GD_4K$-AMC, whereas it has a $K_M$ of 0.5 to 0.6 mM and a $k_{cat}$ of 25 $sec^{-1}$ for $GD_4K$-NA. The optimum pH of $GD_4K$-AMC hydrolysis was pH 8.0. Our data indicate that $GD_4K$-AMC is more suitable as a substrate for enteropeptidase than $GD_4K$-NA.

Thermoluminescence from X-Ray Irradiated Beta-Eucryptite (X-선 조사된 Beta-eucryptite의 열자극 발광)

  • 김태규;이병용;최범식;강현식;추성실;황정남
    • Progress in Medical Physics
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    • v.3 no.1
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    • pp.9-18
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    • 1992
  • In this study, beta-eucryptite is fabricated and the thermal parameters of this material have been investigated. The thermoluminescence from 4MeV X-ray irradiated beta-eucryptite have been measured over the temperature range of 300K-600K. Thermoluminescence curve from X-ray irradiated beta-eucryptite shows five peaks located at 342K, 392K, 438K, 474K, and 527K. $\tau$, $\delta$ and $\omega$ of peak at 527K are 35K, 39K and 74K, respectively and this peak is found to be 2nd order kinetics. The activation energy of peak shape method is calculated to be 1.03eV and the frequency factor for 527K curve is calculated to be 3.9x10$\^$8/sec$\^$-1/. Based on the various heating rates methods, the activation energy of the peak is computed to be 1.02${\pm}$0.05eV that is similar to 1.19${\pm}$0.03eV of initial rise method. The linearity of thermoluminescence intensity and radiation flux is valid up to 50Gy and beyond higher dose the supralinearity and saturation come out.

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