Browse > Article
http://dx.doi.org/10.5713/ajas.2006.1342

Prevalence of Fimbrial Antigen (K88 variants, K99 and 987P) of Enterotoxigenic Escherichia Coli from Neonatal and Post-weaning Piglets with Diarrhea in Central China  

Wang, J. (Key Laboratory of Swine Breeding and Genetics of Agricultural Ministry, College of Animal Scince Huazhong Agricultural University)
Jiang, S.W. (Key Laboratory of Swine Breeding and Genetics of Agricultural Ministry, College of Animal Scince Huazhong Agricultural University)
Chen, X.H. (Key Laboratory of Swine Breeding and Genetics of Agricultural Ministry, College of Animal Scince Huazhong Agricultural University)
Liu, Z.L. (Key Laboratory of Swine Breeding and Genetics of Agricultural Ministry, College of Animal Scince Huazhong Agricultural University)
Peng, J. (Key Laboratory of Swine Breeding and Genetics of Agricultural Ministry, College of Animal Scince Huazhong Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.19, no.9, 2006 , pp. 1342-1346 More about this Journal
Abstract
Enterotoxigenic Escherichia coli is a major cause of diarrhea in neonatal and post-weaning piglets. To determine the most common fimbrial antigens of ETEC in piglets with diarrhea, two investigations were carried out on intensive pig farms in Hubei province, central China. In 2002-2003, 227 fecal samples from neonatal and post-weaning piglets with diarrhea were tested for the presence of the fimbrial antigen K88 and K99 of ETEC by the polymerase chain reaction (PCR). Twenty-three (10.1%) of 227 fecal samples were found to contain fimbrial antigen K88, which was identified as K88ac variant; and 13 (5.7%) samples containing K99. In 2004, another 179 fecal samples from diarrheic piglets, 1 day to 6 weeks of age, were tested for prevalence of fimbrial antigen K88, K99 and 987P. Forty-seven (26.3%) of the 179 samples carried at least one of the ETEC fimbrial antigens. K88 antigen was detected in 20.1%. In the 36 samples known to carry fimbrial antigen K88, 32 (88.9%) contained K88ad; and 4 (11.1%) contained K88ac; none of them carried K88ab. Fimbrial antigens K99 and 987P were detected in 1.1% and 6.1%, respectively. Our data indicate that K88 is the most common fimbrial antigen of ETEC associated with diarrhea in piglets in Central China.
Keywords
Enterotoxigenic Escherichia Coil; Fimbrial Antigen; PCR; Piglets; Diarrhea;
Citations & Related Records

Times Cited By Web Of Science : 4  (Related Records In Web of Science)
Times Cited By SCOPUS : 4
연도 인용수 순위
1 Evelyn, A. D., C. W. Shannon and H. W. Moon. 1989. Agespecific colonization of porcine intestinal epithelium by 987Ppiliated enterotoxigenic Escherchia coli. Infect. Immun. 57:82-87
2 Nagy, B., T. A. Casey and H. W. Moon. 1990. Phenotype and Genotype of Escherichia coli isolated from pigs with postweaning diarrhea in hungry. J. Clin. Microbiol. 28:651-653
3 Natro, J. P. and J. B. Kaper. 1998. Diarrheagenic Escherchia coli. Clin. Microbiol. Rev. 11:142-201
4 Ojeniyi, B., P. Ahrens and A. Meyling. 1994. Detection of fimbrial and toxin genes in and their prevalence in piglets with diarrhea. The applfication of colony hybridization assay, polymerase chain reaction and phenotype assays. J. Vet. Med. B 41:49-59   DOI   ScienceOn
5 Spreeuwenberg, M. A. M., J. M. A. J. Verdonk, H. R. Gaskins and M. W. A. Verstegen. 2001. Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning. J. Nutr. 131:1520-1527
6 Hampson, D. J. 1994. Post-weaning Escherichia coli diarrhea in pigs. In: (Ed. C. L. Gyles) in domestic animals and humans. CAB International, Wallingfird, UK. pp. 629-647
7 Broeck, W. V. D., E. Cox, B. Oudega and B. M. Goddeeris. 2000. The F4 fimbrial antigen of Escherichia coli and its receptors. Vet. Microbiol. 71:223-244   DOI   ScienceOn
8 Garabal, J. I., F. Vazquez, J. Blanco, M. Blanco and E. A. Gonzalez. 1997. Colonization antigens of enterotoxigenic Escherichia coli strains isolated from piglets in Spain. Vet. Microbiol. 54:321-328   DOI   ScienceOn
9 Alexander, T. J. L. 1994. Neonatal diarrhea in pigs. In: (C. L. Gyles) in domestic animals and humans. CAB International, Wallingfird, UK, pp. 151-170
10 Evans, D. G., D. J. Evans, Jr. and W. Tjoa. 1977. Hemaggluation of human group A erythrocyte by enterotoxigenic Escherichia coli isolated from adults with diarrhea: Correlation with colonization factor. Infect. Immun. 18:330-337
11 Harel, J., H. Lapinte, A. Fallara, L. A. Lortie, M. Bigras-Poulin, S. Lariviere and J. M. Fairbrother. 1991. Detection of genes for fimbrial antigens and enterotoxins associated with Escherichia coli serogroups isolated from pigs with diarrhea. J. Clin. Microbiol. 29:745-752
12 Moon, H. W., A. Robert, B. S. Schneider and L. Steve. 1986. Comparative prevalence of four enterotoxins genes among Escherichia coli isolated from swine. Am. J. Vet. Res. 47:210-212
13 Hua, R. H., S. X. Zhang, K. W. He, Y. X. Ni and Z. Z. Yang. 2002. Detection of 987P positive enterotoxigenic Escherichia coli by polymerase chain reaction. Chi. J. Vet. Sci. Tech. 32:3-5
14 Jin, L. Z. and X. Zhao. 2000. Intestinal receptors for adhesive fimbriae of enterotoxigenic Escherichia coli (ETEC) K88 in swine - a review. Appl. Microbiol. Biotchnol. 54:311-318   DOI
15 Wilson, R. A. and D. H. Francis. 1986. Fimbriae and enterotoxins associated with Escherichia coli serogroups isolated from pigs with colibacillosis. Am. J. Vet. Res. 2:213-217
16 Franklin, M. A., D. H. Francies and A. G. Mathew. 1996. A PCRbased method of detection and differentiation of $K88^+$ adhesive Escherichia coli. J. Vet. Dian. Invest. 8:460-463   DOI   ScienceOn
17 Osek, J. 1999. Prevalence of virulence factors of Escherichia coli strains isolated from diarrheic and healthy piglets after weaning. Vet. Microbiol. 68:209-217   DOI   ScienceOn
18 Kwon, D., O. Kim and C. Chae. 1999. Prevalence of genotypes for fimbriae and enterotoxins, and of O serogroups in Escherichia coli isolated from diarrheic piglets in Korea. J. Vet. Diagn. Invest. 11:146-151   DOI   ScienceOn
19 Evelyn, A. D. and E. S. James. 1994. Age-related resistance to 987P fimbria mediated colonization correlates with specific glycolipid receptors in intestinal mucus in swine. Infect. Immun. 62:4789-4794
20 Choi, C. S. and C. H Chae, 1999. Genotypic prevalence of F4 variants (ab, ac, and ad) in Escherichia coli isolated from diarrheic piglets in Korea. Vet. Microbiol. 67:307-310   DOI   ScienceOn
21 Soderlind, O., B. Thafvelin and R. Mollby. 1988. Virulence factors in Escherichia coli strains isolate from Swedish piglets with diarrhea. J. Clin. Microbiol. 26:879-884
22 Hong, J. W., O. S. Kwon, B. J. Min, W. B. Lee, K. S. Shon, I. H. Kim and J. W. Kim. 2004. Evaluation effects of spray-dried egg protein containing specific egg yolk antibodies as a substitute for spray-dried plasma protein or antibiotics in weaned pigs. Asian-Aust. J. Anim. Sci. 17:1139-1144   DOI
23 Guinee, P. A. M. and W. H. Jansen. 1979. Behavior of Escherichia coli K antigens K88ab, K8ac, and K88ad in immunoelectrophoresis, double diffusion, and hemagglutination. Infect. Immun. 3:700-705