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Induction of Boiling Stable Proteins by Cold and ABA Treatment in Radish Cotyledon and Hypocotyl (무 자엽과 하배축에서 저온과 ABA처리로 유도된 중탕에 강한 단백질 분석)

  • Cho, Bong-Heuy
    • Analytical Science and Technology
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    • v.13 no.3
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    • pp.346-350
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    • 2000
  • Boiling stable proteins of 53 kDa and 29 kDa existed natively in the cotyledons of Bak Kyoung, fall radish (Raphanus raphanistrodes L.) Boiling stable proteins of 36 kDa and 16.5 kDa were newly induced by cold stress and the proteins of 53 kDa and 29 kDa increased during the cold stress. The proteins of 53 kDa were denatured within 2 hrs after removing cotyledons from plants. Boiling stable proteins of 53 kDa existed natively in the hypocotyls as much as in the cotyledons whereas 24 kDa and 18 kDa proteins were increased by stress. Boiling stable proteins of 53 kDa were induced and those of the 25 kDa and 23 kDa were increased by cold treatment and ABA treatment in the cotyledons of Jangchundaehyung F1 spring white (Raphanus raphanistrodes L.). These results showed the differences of induced boiling stable proteins between fall radishes and spring radishes. Cycloheximide inhibited the induction of 25 kDa and 23 kDa proteins during stress. 22 kDa native protein disappeared during ABA treatment and reappeared by cycloheximide treatments. It may be explained that cycloheximide was responsible for the destruction process of proteins in the living organisms. The profile of boiling stable proteins in hypocotyls of spring radishes during stress was same as that of fall redishes.

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Spatial Optical Modulator (SOM);Samsung's Light Modulator for the Next Generation Laser Display

  • Yun, Sang-Kyeong;Song, Jong-Hyeong;Lee, Tae-Won;Yeo, In-Jae;Choi, Yoon-Joon;Lee, Yeong-Gyu;An, Seung-Do;Han, Kyu-Bum;Victor, Yurlov;Park, Heung-Woo;Park, Chang-Su;Kim, Hee-Yeoun;Yang, Jeong-Suong;Cheong, Jong-Pil;Ryu, Seung-Won;Oh, Kwan-Young;Yang, Haeng-Seok;Hong, Yoon-Shik;Hong, Seok-Kee;Yoon, Sang-Kee;Jang, Jae-Wook;Kyoung, Je-Hong;Lim, Ohk-Kun;Kim, Chun-Gi;Lapchuk, Anatoliy;Ihar, Shyshkin;Lee, Seung-Wan;Kim, Sun-Ki;Hwang, Young-Nam;Woo, Ki-Suk;Shin, Seung-Wan;Kang, Jung-Chul;Park, Dong-Hyun
    • 한국정보디스플레이학회:학술대회논문집
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    • 2006.08a
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    • pp.551-555
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    • 2006
  • A new type of diffractive spatial optical modulators, named SOM, has been developed by Samsung Electro-Mechanics for projection display and other applications. A laser display in full HD format $(1920{\times}1080)$ was successfully demonstrated by using prototype projection engines having SOM devices, signal processing circuits, and projection optics.

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Effects of Dietary Kugija Lycium chinense Supplementation on Juvenile Korean Rockfish Sebastes schlegeli (치어기 조피볼락 사료내 구기자 첨가효과)

  • Lim, Dong-Kuy;Yoo, Kwang-Yeol;Shin, Dong-Gun;Kim, Jong-Eung;Bae, Jun-Young;C. Bai, Sung-Chul;Lee, Jeong-Yeol
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.3
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    • pp.250-256
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    • 2009
  • A 8-week feeding trial was conducted to investigate the effects of dietary supplementation of Kugija (Lycium chinense) on the growth and immunological response in juvenile Korean rockfish (Sebastes schlegeli). Six experimental diets were supplemented with Kugija at 0, 0.1, 0.5 1.0, 3.0 and 5.0% ($K_0,\;K_{0.1},\;Ko_{0.5},\;K_{1.0},\;K_{3.0},\;K_{5.0}$) on a dry-matter basis. After 2 weeks, triplicate groups of 30 fish initially averaging 3.36$\pm$0.2 g (mean$\pm$SD) were randomly distributed into the aquarium and were fed one of the experimental diets for 8 weeks. By the end of the 8-week feeding trial, fish fed the $K_{0.5}$ and $K_{1.0}$ diets exhibited a higher weight gain and specific growth rate than fish fed $K_0$ and $K_{0.1}$ diets (P<0.05). Feed efficiency of fish fed the $K_{0.5}$ diet showed significant higher value than that of fish fed the $K_0$, $K_{3.0}$ and $K_{5.0}$ diets (P<0.05). Hepatosomatic index of fish fed the $K_{0.5}$ diet was significantly higher than that of fish fed the $K_{0.1}$ and $K_{5.0}$ diets (P<0.05). Hematocrit of fish fed the $K_{0.5},\;K_{1.0}$ and $K_{5.0}$ diets was significantly higher than that of fish fed the $K_0$ diet (P<0.05). Glutamic oxaloacetic transaminase of fish fed the $K_{0.5},\;K_{1.0},\;K_{3.0}$ and $K_{5.0}$ diets was significantly lower than in fish fed the other diets (P<0.05). Glutamic pyruvic transaminase activity of fish fed the $K_{3.0}$ diet was significantly lower than those of fish fed the $K_0$ and $K_{0.1}$ diets (P<0.05). However, there was no significant difference in the activity of the transaminase in fish fed the $K_{0.5}\;K_{1.0},\;K_{3.0}$ and $K_{5.0}$ diets. Lysozyme activity of fish fed the $K_{0.5}$ and $K_{1.0}$ diets was significantly higher than that in fish fed the $K_0$ and $K_{0.1}$ diets (P<0.05). Respiratory burst activity of fish fed the $K_{3.0}$ diet was significantly higher than those of fish fed the $K_0$ and $K_{0.1}$ diets (P<0.05). However, there was no significant difference in respiratory burst activity of fish fed the $K_{0.5}\;K_{1.0},\;K_{3.0}$ and $K_{5.0}$ diets. Fish fed Kugija showed lower early mortality than fish fed a Kugija-free diet upon challenge with Vibrio ordalii. The results suggest that feeding of Kugija (Lycium chinense) enhances growth, non-specific immunity and disease resistance in juvenile Korean rockfish.

Isolation and Characterization of Two Mosquitocidal Bacillus thuringien- sis Strains Belonging to subsp. kurstaki and subsp. aizawai

  • Roh, Jong-Yul;Li, Ming-Shun;Chang, Jin-Hee;Shim, Hee-Jin;Jin, Byung-Rae;Je, Yeon-Ho
    • International Journal of Industrial Entomology and Biomaterials
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    • v.3 no.1
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    • pp.19-23
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    • 2001
  • Two B. thuringiensis strains, which possess mosquitocidal activities, were isolated from Korean soil samples and named K-1205-1 and K-1381-1. Serological studies indicated that K-1205-1 and K-1381-1 belonged to B. thuringiensis subsp. kurstaki (H3a3b3c) and subsp. aizawai (H7), respectively. K-1205-1 produced typical bipyramidal parasporal inclusions, but K-1381-1 produced irregular bipyramidal shape. Total plasmid DNA patterns analysis shewed that K-1205-1 and K- 1381-1 were different from their reference strains, subsp. kurstaki and subsp. aizawai, respectively, in high molecules, whereas their crystal protein patterns showed no difference. The cry gene contents of K-1205-1 and K-1381-1 were identical with those of the reference strains. Mosquitocidal activities of crystal proteins produced by K-1205-1 and K-1381-1 were significantly high by about 40-50 folds at $LC_50$ when compared to those of subsp. kurstaki and subsp. aizawai. Finally, in southern blot analysis using cry1A-type specific probe, K-1205-1 and K-1381-1 had different bands from subsp. kurstaki and subsp. aizawai, respectively. In conclusion, our results suggest that K-1205-1 and K-1381-1 appear to be new moquitocidal B. thuringiensis strains isolated from Korean soil.

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The Effect of Soil Water Content during at Fruit Ripening Stage on Yield and Quality in Musk melon (머스크멜론의 과실성숙기 토양수분이 수량과 품질에 미치는 영향)

  • 권준국;이재한;엄영철;김회태;최영하;박동금
    • Journal of Bio-Environment Control
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    • v.7 no.4
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    • pp.330-335
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    • 1998
  • To investigate the effect of soil water control on yield and quality of musk melon in plastic film house, irrigation points were treated with -10, -20, -30, -50 and -100 kPa by 10mm dripping each time at fruit developing and ripening stage, respectively. Fresh weight of stem and leaves was not significant among irrigation points, but percentage of dry matter was highest at -100kPa and lowest at -10kPa. Marketable yield was not different among -50kPa, -100kPa, -30kPa and -20kPa and lowest at -10kPa. Sugar content of the flesh fruit at ripening stage was 15.1 $^。/Brix at -50kPa and 14.4 $^。/Brix at -10kPa Therefore, optimum irrigation point at ripening stage of fruit is -50kPa by 10mm dripping each time. time.

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COMPARATIVE STUDY ON PERCENTAGE OF CANAL ENLARGEMENT AND CANAL ANGULATION CHANGE BY PRECURVED ENDOSONIC K-FILE (Precurved Endosonic K-file에 의한 근관확대율 및 만곡도 변화에 대한 연구)

  • Hur, Heuy-Keung;Oh, Won-Mann;Yang, Kyu-Ho
    • Restorative Dentistry and Endodontics
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    • v.20 no.1
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    • pp.316-327
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    • 1995
  • The purpose of this study was to evaluate canal shaping ability and canal angulation change of K-file, straight endosonic K-file and pre curved endosonic K-file. Twenty staight canals and thirty curved canals were selected and divided into five groups according to canals curvature and canal instrumentation method. VI group was prepared by straight endosonic K-file and H1 group by K-file in straigt canals. V2 group was instrumented by straight endosonic K-file, V3 group by pre curved endosonic K-file and H2 group by precurved K-file in curved canals. Radiographs of canals were obtained before and after canal shaping. And postoperative radiographs were compared with preoperative radiographs using superimposition method. The results obtained were as follows ; l. In straight canals, K-file group demonstrated lager percentage of canal enlargement than endosonic K-file group on facial view, but reverse results exhibit on mesial view. 2. In curved canals, precurved K-file group showed largest percentage of canal enlargement, followed by precurved endosonic K-fine group and straight endosonic K-file group was smallest. 3. Percantage of canal enlargement at convex side was greater than at concave side in apical portion of each group. Especially in straight endosonic K-file group, percantage of canal enlargement at convex side and concave side showed sharply difference. 4. In angulation change, the straight endosonic K-file group exhibited the greatest its change, followed by precurved endosonic K-file group and precurved K-file group was the least. Above results suggest that K-file is more effective endodontic instrument than endosonic K-file, and that precurved file is effective for canal shaping in curved canal.

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Histone H3K27 Modifications and Gene Transcription (히스톤 H3K27 변형과 유전자 전사)

  • Kim, Ae-Ri
    • Journal of Life Science
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    • v.21 no.4
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    • pp.616-620
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    • 2011
  • Lysine residues of histone H3 and H4 are covalently modified in the chromatin of eukaryotic cells. Lysine 27 in histone H3 was acetylated (H3K27ac) or methylated at three levels; mono-, di-, and trimethylation (H3K27me1, H3K27me2, and H3K27me3). These modifications at H3K27 were related with gene transcription and/or chromatin structure in distinct patterns. Generally, H3K27ac and H3K27me1 were enriched in active chromatin, such as the locus control region or transcriptionally active genes, while transcriptionally inactive genes were highly marked by H3K27me2 and H3K27me3. These modifications appear to have been catalyzed by distinct histone-modifying enzymes. Recent studies suggest that the four kinds of modifications at H3K27 have inter-correlation in gene transcription or chromatin structure formation.