• Title/Summary/Keyword: K^+$ currents

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Inwardly Rectifying $K^+$ Currents in Gastric Myocytes of Guinea-pig

  • Jun, Jae-Yeoul;Yeum, Cheol-Ho;Yoon, Pyung-Jin;Jang, In-Youb;Cho, Nam-Soo;Cho, Soo-Hyeong;Kong, In-Deok;Kim, Tae-Wan;So, In-Suk;Kim, Ki-Whan
    • The Korean Journal of Physiology and Pharmacology
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    • v.6 no.1
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    • pp.47-55
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    • 2002
  • To identify the presence of inwardly rectifying $K^+$ channels and its characteristics, membrane currents were measured using a whole-cell patch clamp from isolated gastric myocytes of guinea-pig. Change of external $K^+$ concentration from 5 to 90 mM induced an inward current at a holding potential of -80 mV. The high $K^+-induced$ inward current was blocked by $Ba^{2+}$ and $Cs^+,$ but not by glibenclamide. With 90 mM $K^+$ in bath, the $Ba^{2+}-$ and $Cs^+-sensitive$ currents showed strong inward rectification. Ten mM TEA weakly blocked the inward current only at potentials more negative than -50 mV. With 90 mM $K^+$ in bath, hyperpolarizing step pulses from -10 mV induced inward currents, which were inactivated at potentials more negative than -70 mV. Reduction of external $K^+$ to 60 mM decreased the amplitudes of the currents and shifted the reversal potential to more negative potential. The inactivation of inward $K^+$ current at negative clamp voltage was not affected by removing external $Na^+.$ These results suggest that the inwardly rectifying $K^+$ channels may exist in gastric smooth muscle.

Calculation of induced currents and analysis of shielding effectiveness in finite conductive shield (도전성 유한 차폐판의 유도전류 계산 및 차폐효과 분석)

  • Kang, Dael-Ha;Son, Jung-Dae;Lee, Yung-Sik;Jo, Yeong-Ho;Choi, Phil-Soo;Park, Sang-Ho;Kim, Won-Hi
    • Proceedings of the KIEE Conference
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    • 2011.07a
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    • pp.551-552
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    • 2011
  • In this study new evaluation method of eddy currents in conductive shielding sheets are introduced. This method is based on the magnetic flux linkage in rectangular cells. According to this method the allocated amount of memory and the calculation time can be reduced. In this method calculation of magnetic field in any space can be performed by summing contributions of source currents and eddy currents in conductive shielding sheets. This method is applicable to the design of magnetic field-shield.

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Analysis of Harmonic Currents Propagation on the Self-Excited Induction Generator with Nonlinear Loads

  • Nazir, Refdinal
    • Journal of Electrical Engineering and Technology
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    • v.9 no.6
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    • pp.1935-1943
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    • 2014
  • In recent years, the induction machines are increasingly being used as self-excited induction generators (SEIG). This generator is especially widely employed for small-scale power plants driven by renewable energy sources. The application of power electronic components in the induction generator control (IGC) and the loading of SEIG using nonlinear loads will generate harmonic currents. This paper analyzes the propogation of harmonic currents on the SEIG with nonlinear loads. Transfer function method in the frequency domain is used to calculate the gain and phase angle of each harmonic current component which are generated by a nonlinear loads. Through the superposition approach, this method has also been used to analyze the propagation of harmonic currents from nonlinear load to the stator windings. The simulation for the propagation of harmonic currents for a 4 pole, 1.5 kW, 50Hz, 3.5A, Y-connected, rotor-cage SEIG with energy-saving lamps, have provided results almost the same with the experiment. It can prove that the validity of the proposed models and methods. The study results showed that the propagation of harmonic currents on the stator windings rejects high order harmonics and attenuates low order harmonics, consequently THDI diminish significantly on the stator windings.

Remote Sensing of Nearshore Currents using Coastal Optical Imagery (해안 광학영상 자료를 이용한 쇄파지역 연안류 측정기술)

  • Yoo, Jeseon;Kim, Sun-Sin
    • Ocean and Polar Research
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    • v.37 no.1
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    • pp.11-22
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    • 2015
  • In-situ measurements are labor-intensive, time-consuming, and limited in their ability to observe currents with spatial variations in the surf zone. This paper proposes an optical image-based method of measurement of currents in the surf zone. This method measures nearshore currents by tracking in time wave breaking-induced foam patches from sequential images. Foam patches in images tend to be arrayed with irregular pixel intensity values, which are likely to remain consistent for a short period of time. This irregular intensity feature of a foam patch is characterized and represented as a keypoint using an image-based object recognition method, i.e., Scale Invariant Feature Transform (SIFT). The keypoints identified by the SIFT method are traced from time sequential images to produce instantaneous velocity fields. In order to remove erroneous velocities, the instantaneous velocity fields are filtered by binding them within upper and lower limits, and averaging the velocity data in time and space with a certain interval. The measurements that are obtained by this method are comparable to the results estimated by an existing image-based method of observing currents, named the Optical Current Meter (OCM).

A Safety Analysis of Electric Detonator for Stray Currents by Domestic Transmission Network System (국내 송전계통별 미주전류에 의한 전기뇌관의 안전성 분석)

  • Park Hyun-Sik;Kim Young-Seok;Kang Choo-Won
    • Tunnel and Underground Space
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    • v.15 no.4 s.57
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    • pp.296-304
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    • 2005
  • This study is to observe stray currents generated around the steel tower by domestic transmission network system and analysis stability of electric detonator. It is measured the stay current of each ten place at steel tower of 765 kV, 345 tV, 154 tV transmission line among domestic transmission network system. Stay currents measured a total of 40m at intervals of 4m toward a line direction and a line vertical direction centering around steel tower. Temperature of the surface, EC, water content also are measured. Although stay currents show the highest values, that is 12 percent of at 4m and less than 1 percent of 40m with Institute of Makers of Explosives(IME) regulations. It is shown correlation between stay currents and water content$\cdot$EC$\cdot$temperature of the surface. Stay currents measured at line direction and line vertical direction were little different and the shape of diminution was also shown a similar aspect.

Electrophysiological Properties of the Neurons Dissociated from the Nucleus Raphe Magnus in Postnatal Rats (흰쥐의 Nucleus Raphe Magnus로부터 분리된 신경세포의 전기생리학적 성질)

  • Nam Sang-Chae;Lim Won-Il;Cho Sa-Sun;Kim Jun
    • The Korean Journal of Physiology and Pharmacology
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    • v.1 no.3
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    • pp.233-240
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    • 1997
  • Neurons in the nucleus raphe magnus are involved in descending modulation of nociceptive transmission. In this study, we attempted to investigate electrophysiological properties of the NRM neurons dissociated from the postnatal rat medulla. The NRM neurons in the coronal slices of and the dissociated neurons from the postnatal rat medullae were immunohistochemically identified using antibody against serotonin. Relatively small number of neurons were positively stained in both preparations. The positively stained neurons displayed large cell body with double or multiple neurites. Using whole-cell patch clamp configuration ionic currents were recorded from the dissociated NRM-like neurons selected by criteria such as size and shape of cell body and cell population. Two types, high- and low-threshold, of voltage-dependent calcium currents were recorded from the dissociated NRM-like neurons. Some neurons displayed both types of calcium currents, whereas others displayed only high-threshold calcium current. Voltage-dependent potassium currents were also recorded from the dissociated NRM neurons. Some neurons displayed both transient outward and delayed rectifier currents but others showed only delayed rectifier current. These results suggest that there are at least two types of calcium currents and two types of potassium currents in the dissociated NRM neurons.

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Wave-induced Currents using XBEACH Model after Beach Nourishment at Haeundae Beach (XBEACH 모형에 의한 해운대 해수욕장 양빈후의 해빈류 특성 변화)

  • Kang, Tae-Soon;Park, Myeong-Won;Kim, Jin-Seok;Lee, Jong-Sup
    • Journal of Ocean Engineering and Technology
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    • v.30 no.6
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    • pp.498-504
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    • 2016
  • In this study, to predict the effect of beach nourishment at Haeundae Beach, the waves and wave-induced currents were compared before and after beach nourishment using the XBEACH model. Representative wave conditions were determined for the data observed during 2014 (KHOA). Then, the Hs,max and Hs,1/10 values, and their prevalent directions, were used in the numerical modeling input data. A variable grid system was used for the $5km{\times}2.5km$ model areas, and irregular waves based on the JONSWAP spectrum were given as incident wave conditions. In the summer season, eastward wave-induced currents were developed along the beach by the incident wave direction. Before the beach nourishment, the maximum speed around the surf zone was 1.2-1.5 m/s in the central zone of the beach, whereas the maximum speed increased to 1.4-1.6 m/s at the same areas when the currents toward Mipo Harbor were blocked as an effect of the groins after the beach nourishment. In the winter season, westward wave-induced currents were developed along the beach by the incident wave direction. After the beach nourishment, the maximum current speed increased slightly around the surf zone in the central area of the beach, and the littoral current speed decreased at the submerged breakwaters located at Dongbaek Island. As a result, after the beach nourishment, the maximum wave-induced currents increased about 10% in the surf zone of the central area of the beach.

External pH Effects on Delayed Rectifier $K^+$ Currents of Small Dorsal Root Ganglion Neuron of Rat

  • Kim, Young-Ho;Hahn, Jung-Hyun;Lim, In-Ja;Chung, Sung-Kwon;Bang, Hyo-Weon
    • The Korean Journal of Physiology and Pharmacology
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    • v.2 no.2
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    • pp.165-172
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    • 1998
  • Under certain pathophysiological conditions, such as inflammation and ischemia, the concentration of H^+$ ion in the tissue surrounding neurons is changed. Variations in H^+$ concentration are known to alter the conduction and/of the gating properties of several types of ion channels. Several types of K^+$ channels are modulated by pH. In this study, the whole cell configuration of the patch clamp technique has been applied to the recording of the responses of change of external pH on the delayed rectifier K^+$ current of cultured DRG neurons of rat. Outward K^+$ currents were examined in DRG cells, and the Charybdotoxin and Mn^{2+}$ could eliminate Ca^{2+}-dependent$ K^+$ currents from outward K^+$ currents. This outward K^+$ current was activated around -60 mV by step depolarizing pulses from holding potential -70 mV. Outward K^+$ currents were decreased by low external pH. Activation and steady-state inactivation curve were shifted to the right by acidification, while there was small change by alkalization. These results suggest that H^+$ could be alter the sensory modality by changing and modifying voltage-dependent K^+$ currents, which participated in repolarization.

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Characteristic Changes of ZnO Arrester Blocks by Multiple-lightning Impuse Currents (다중 뇌충격전류에 의한 산화아연형 피뢰기 소자의 특성 변화)

  • Gil, Gyeong-Seok;Han, Ju-Seop
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.49 no.12
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    • pp.685-690
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    • 2000
  • Multiple-lightning impulse currents are a general feature of the lightning ground f=flash. It is therefore necessary for lightning arresters used in power systems to be estimated by applying not only a single-lightning impulse current but also a multiple-lightning impulse currents. This paper presents the effects of multiple-lightning impulse currents on deterioration of ZnO arrester blocks. The multiple-lightning impulse generator which can produce quadruple 8/20$[\mus]$ 5[kA] with separation time of 30~120[ms] is designed and fabricated. The total energy applied to the arrester block at each impulse is about 1,200[J]. In experiment, various parameters such as leakage current component, reference voltage, and temperature are measured with the number of applied impulse current. Also, micro-structure changes of the ZnO blocks after applying the single and the multiple-lightning impulse currents of 200 times are compared. The experimental results indicate that the types of arrester blocks are more vulnerable to deterioration or damage by multiple-lightning impulse currents.

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Mechanism of Membrane Hyperpolarization by Extracellular $K^+$ in Resistance-sized Cerebral Arterial Muscle Cell of Rabbit

  • Kim, Se-Hoon;Choi, Kun-Moo;Kim, Hoe-Suk;Jeon, Byeong-Hwa;Chang, Seok-Jong
    • The Korean Journal of Physiology and Pharmacology
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    • v.3 no.1
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    • pp.1-10
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    • 1999
  • We sought to find out the mechanism of vascular relaxation by extracellular $K^+$ concentration $([K^+]_o)$ in the cerebral resistant arteriole from rabbit. Single cells were isolated from the cerebral resistant arteriole, and using voltage-clamp technique barium-sensitive $K^+$ currents were recorded, and their characteristics were observed. Afterwards, the changes in membrane potential and currents through the membrane caused by the change in $[K^+]_o$ was observed. In the smooth muscle cells of cerebral resistant arteriole, ion currents that are blocked by barium, 4-aminopyridine (4-AP), and tetraethylammonium (TEA) exist. Currents that were blocked by barium showed inward rectification. When the $[K^+]_o$ were 6, 20, 60, and 140 mM, the reversal potentials were $-82.7{\pm}1.0,\;-49.5{\pm}1.86,\;-26{\pm}1.14,\;-5.18{\pm}1.17$ mV, respectively, and these values were almost identical to the calculated $K^+$ equilibrium potential. The inhibition of barium-sensitive inward currents by barium depended on the membrane potential. At the membrane potentials of -140, -100, and -60 mV, $K_d$ values were 0.44, 1.19, and 4.82 ${\mu}M,$ respectively. When $[K^+]_o$ was elevatedfrom 6 mM to 15 mM, membrane potential hyperpolarized to -50 mV from -40 mV. Hyperpolarization by $K^+$ was inhibited by barium but not by ouabain. When the membrane potential was held at resting membrane potential and the $[K^+]_o$ was elevated from 6 mM to 15 mM, outward currents increased; when elevated to 25 mM, inward currents increased. Fixing the membrane potential at resting membrane potential and comparing the barium-sensitive outward currents at $[K^+]_o$ of 6 and 15 mM showed that the barium- sensitive outward current increased at 15 mM $K^+.$ From the above results the following were concluded. Barium-sensitive $K^+$?channel activity increased when $[K^+]_o$ is elevated and this leads to an increase in $K^+-outward$ current. Consequently, the membrane potential hyperpolarizes, leading to the relaxation of resistant arteries, and this is thought to contribute to an increase in the local blood flow of brain.

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